Preparo de padr?es anal?ticos, estudo de estabilidade e par?metros de valida??o para ensaio de caroten?ides por cromatografia l?quida
Ano de defesa: | 2009 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal Rural do Rio de Janeiro
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos
|
Departamento: |
Instituto de Tecnologia
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://tede.ufrrj.br/jspui/handle/tede/414 |
Resumo: | Vitamin A is essential to the human beings, being ingested through animal sources as meat and eggs, or gotten for the conversion of pro vitamin carotenoids found mainly in fruits and vegetables. The vitamin A deficiency is the major nutritional problem that affects underdeveloped populations. As a consequence of this deficiency, xerophthalmia, blindness and premature death can occur, mainly in children. Vitamin A is also important for growth maintenance, for reproductive efficiency, maintenance of the epithelial tissues and keratinization prevention, beyond presenting an important role into the immunological response. Nowadays, the deficient intake of vitamin A is the major nutritional problem found into developing countries populations or poor population of the developed countries. Around of 4.4 million of children, within preschool age, are presenting vision damages due to the deficiency of this vitamin, every year about 250-500 thousand children become blind. Carotenoids are the most important source of retinol for populations in which the consumption of this vitamin is low. For this reason, quantitative and qualitative analysis of carotenoids in foods is very important. In this work, a specific chromatographic methodology was developed to separate the carotenoids and their isomers. Chromatographic separation of the main food carotenoids was optimized and validated. The chromatographic standards were isolated with appropriated amount and purity, and their stability was evaluated to determine the better conditions of storage, as well as shelf life. The isolation of all chromatographic standards was carried out with open column, quantification was done using spectrophotometry and purity evaluation was measured by HPLC. Many matrices have been evaluated as a source of standards, some new sources were found and showed better characteristics, as purity higher than 90%, if compared with those, frequently, used for this purpose. The study of stability showed that mixtures of carotenoids used as external standard for calibration can be storage more than 370 days. Validation study showed that this methodology of analysis has good selectivity, sensitivity and linearity in a concentration range from 0.5 to 8.0 ?g/mL for each carotenoid. |
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Godoy, Ronoel Luiz de Oliveira507.802.047-00http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786632H6820.443.339-53http://lattes.cnpq.br/1148865776679739Pacheco, Sidney2016-04-28T14:54:40Z2010-12-062009-03-12PACHECO, Sidney. Preparo de padr?es anal?ticos, estudo de estabilidade e par?metros de valida??o para ensaio de caroten?ides por cromatografia l?quida. 2009. 109 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2009.https://tede.ufrrj.br/jspui/handle/tede/414Vitamin A is essential to the human beings, being ingested through animal sources as meat and eggs, or gotten for the conversion of pro vitamin carotenoids found mainly in fruits and vegetables. The vitamin A deficiency is the major nutritional problem that affects underdeveloped populations. As a consequence of this deficiency, xerophthalmia, blindness and premature death can occur, mainly in children. Vitamin A is also important for growth maintenance, for reproductive efficiency, maintenance of the epithelial tissues and keratinization prevention, beyond presenting an important role into the immunological response. Nowadays, the deficient intake of vitamin A is the major nutritional problem found into developing countries populations or poor population of the developed countries. Around of 4.4 million of children, within preschool age, are presenting vision damages due to the deficiency of this vitamin, every year about 250-500 thousand children become blind. Carotenoids are the most important source of retinol for populations in which the consumption of this vitamin is low. For this reason, quantitative and qualitative analysis of carotenoids in foods is very important. In this work, a specific chromatographic methodology was developed to separate the carotenoids and their isomers. Chromatographic separation of the main food carotenoids was optimized and validated. The chromatographic standards were isolated with appropriated amount and purity, and their stability was evaluated to determine the better conditions of storage, as well as shelf life. The isolation of all chromatographic standards was carried out with open column, quantification was done using spectrophotometry and purity evaluation was measured by HPLC. Many matrices have been evaluated as a source of standards, some new sources were found and showed better characteristics, as purity higher than 90%, if compared with those, frequently, used for this purpose. The study of stability showed that mixtures of carotenoids used as external standard for calibration can be storage more than 370 days. Validation study showed that this methodology of analysis has good selectivity, sensitivity and linearity in a concentration range from 0.5 to 8.0 ?g/mL for each carotenoid.A vitamina A ? essencial aos seres humanos sendo ingerida atrav?s de alimentos de origem animal como carnes e ovos, ou obtida atrav?s da convers?o dos caroten?ides pr?-vitam?nicos presentes principalmente em frutas e vegetais. A defici?ncia de vitamina A (retinol) ? o maior problema nutricional em popula??es subdesenvolvidas e suas conseq??ncias s?o a xeroftalmia, cegueira e morte prematura, especialmente em crian?as. A vitamina A ? tamb?m importante na manuten??o do crescimento, na efici?ncia reprodutiva, na manuten??o dos tecidos epiteliais e na preven??o de queratiniza??o, al?m de apresentar uma importante a??o na resposta imunol?gica. A ingest?o deficiente de vitamina A, especialmente em popula??es de pa?ses subdesenvolvidos ou popula??es pobres de pa?ses desenvolvidos, ? o maior problema nutricional da atualidade. Cerca de 4,4 milh?es de crian?as em idade pr?-escolar apresentam danos ? vis?o devido ? defici?ncia desta vitamina e anualmente 250 a 500 mil dessas crian?as acabam cegas. Os caroten?ides s?o a fonte de retinol mais importante em popula??es onde a ingest?o dessa vitamina ? baixa. Desta maneira, a sua correta an?lise, tanto qualitativa quanto quantitativa, em alimentos ? muito importante. Neste trabalho, desenvolveu-se uma metodologia cromatogr?fica espec?fica para a separa??o dos caroten?ides e seus is?meros. Este estudo prop?s a otimiza??o da separa??o cromatogr?fica dos principais caroten?ides encontrados em alimentos e a posterior valida??o do m?todo implantado. Para isto foi necess?rio o isolamento dos padr?es desses caroten?ides em quantidades e purezas suficientes. Uma avalia??o da estabilidade dos padr?es preparados foi realizada para determinar as melhores condi??es de armazenamento, assim como o per?odo m?ximo de armazenamento. O isolamento dos padr?es deu-se por cromatografia aberta com posterior quantifica??o espectrofotom?trica e determina??o da pureza atrav?s de CLAE. Avaliaram-se diversas matrizes naturais como fonte dos caroten?ides de interesse e foram descobertas novas matrizes com caracter?sticas melhores que as usuais para o isolamento desses padr?es, produzindo padr?es com purezas cromatogr?ficas superiores ? exig?ncia m?nima de 90%. O estudo de estabilidade mostrou que ? poss?vel o armazenamento dos padr?es, em misturas multi padr?es prontas para o uso para calibra??o cromatogr?fica externa, por mais de 370 dias. A valida??o mostrou que o m?todo implantado e otimizado ? seletivo, sens?vel, linear e a faixa de trabalho para a an?lise dos caroten?ides em amostras de alimentos foi estabelecida em 0,5 a 8,0?g/mL para cada caroten?ide.Made available in DSpace on 2016-04-28T14:54:40Z (GMT). No. of bitstreams: 1 2009 - Sidney Pacheco.pdf: 2505724 bytes, checksum: b8aa2986c85643d86695c3e55f0a6528 (MD5) Previous issue date: 2009-03-12application/pdfhttps://tede.ufrrj.br/retrieve/56645/2009%20-%20Sidney%20Pacheco.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncia e Tecnologia de AlimentosUFRRJBrasilInstituto de Tecnologiacaroten?idesvitamina Avalida??o de m?todos anal?ticoscarotenoidsvitamin Avalidation of analytical methodsCi?ncia e Tecnologia de AlimentosPreparo de padr?es anal?ticos, estudo de estabilidade e par?metros de valida??o para ensaio de caroten?ides por cromatografia l?quidaPreparation of analytical standards, study of stability and validation parameters of the test of carotenoids by liquid chromatographyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJORIGINAL2009 - Sidney Pacheco.pdf2009 - Sidney Pacheco.pdfapplication/pdf3968081http://localhost:8080/tede/bitstream/tede/414/19/2009+-+Sidney+Pacheco.pdfd42dafd2d64ce869743f69775cad76c8MD519THUMBNAIL2009 - Sidney Pacheco.pdf.jpg2009 - Sidney Pacheco.pdf.jpgimage/jpeg4205http://localhost:8080/tede/bitstream/tede/414/18/2009+-+Sidney+Pacheco.pdf.jpg1e1bf0003b18b072ef6d361dd5105166MD518TEXT2009 - Sidney Pacheco.pdf.txt2009 - Sidney Pacheco.pdf.txttext/plain200552http://localhost:8080/tede/bitstream/tede/414/17/2009+-+Sidney+Pacheco.pdf.txt95459918a71e2cea7d36e44d05fafd71MD517tede/4142023-07-21 17:55:25.057oai:localhost:tede/414Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2023-07-21T20:55:25Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
dc.title.por.fl_str_mv |
Preparo de padr?es anal?ticos, estudo de estabilidade e par?metros de valida??o para ensaio de caroten?ides por cromatografia l?quida |
dc.title.alternative.eng.fl_str_mv |
Preparation of analytical standards, study of stability and validation parameters of the test of carotenoids by liquid chromatography |
title |
Preparo de padr?es anal?ticos, estudo de estabilidade e par?metros de valida??o para ensaio de caroten?ides por cromatografia l?quida |
spellingShingle |
Preparo de padr?es anal?ticos, estudo de estabilidade e par?metros de valida??o para ensaio de caroten?ides por cromatografia l?quida Pacheco, Sidney caroten?ides vitamina A valida??o de m?todos anal?ticos carotenoids vitamin A validation of analytical methods Ci?ncia e Tecnologia de Alimentos |
title_short |
Preparo de padr?es anal?ticos, estudo de estabilidade e par?metros de valida??o para ensaio de caroten?ides por cromatografia l?quida |
title_full |
Preparo de padr?es anal?ticos, estudo de estabilidade e par?metros de valida??o para ensaio de caroten?ides por cromatografia l?quida |
title_fullStr |
Preparo de padr?es anal?ticos, estudo de estabilidade e par?metros de valida??o para ensaio de caroten?ides por cromatografia l?quida |
title_full_unstemmed |
Preparo de padr?es anal?ticos, estudo de estabilidade e par?metros de valida??o para ensaio de caroten?ides por cromatografia l?quida |
title_sort |
Preparo de padr?es anal?ticos, estudo de estabilidade e par?metros de valida??o para ensaio de caroten?ides por cromatografia l?quida |
author |
Pacheco, Sidney |
author_facet |
Pacheco, Sidney |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Godoy, Ronoel Luiz de Oliveira |
dc.contributor.advisor1ID.fl_str_mv |
507.802.047-00 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786632H6 |
dc.contributor.authorID.fl_str_mv |
820.443.339-53 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1148865776679739 |
dc.contributor.author.fl_str_mv |
Pacheco, Sidney |
contributor_str_mv |
Godoy, Ronoel Luiz de Oliveira |
dc.subject.por.fl_str_mv |
caroten?ides vitamina A valida??o de m?todos anal?ticos |
topic |
caroten?ides vitamina A valida??o de m?todos anal?ticos carotenoids vitamin A validation of analytical methods Ci?ncia e Tecnologia de Alimentos |
dc.subject.eng.fl_str_mv |
carotenoids vitamin A validation of analytical methods |
dc.subject.cnpq.fl_str_mv |
Ci?ncia e Tecnologia de Alimentos |
description |
Vitamin A is essential to the human beings, being ingested through animal sources as meat and eggs, or gotten for the conversion of pro vitamin carotenoids found mainly in fruits and vegetables. The vitamin A deficiency is the major nutritional problem that affects underdeveloped populations. As a consequence of this deficiency, xerophthalmia, blindness and premature death can occur, mainly in children. Vitamin A is also important for growth maintenance, for reproductive efficiency, maintenance of the epithelial tissues and keratinization prevention, beyond presenting an important role into the immunological response. Nowadays, the deficient intake of vitamin A is the major nutritional problem found into developing countries populations or poor population of the developed countries. Around of 4.4 million of children, within preschool age, are presenting vision damages due to the deficiency of this vitamin, every year about 250-500 thousand children become blind. Carotenoids are the most important source of retinol for populations in which the consumption of this vitamin is low. For this reason, quantitative and qualitative analysis of carotenoids in foods is very important. In this work, a specific chromatographic methodology was developed to separate the carotenoids and their isomers. Chromatographic separation of the main food carotenoids was optimized and validated. The chromatographic standards were isolated with appropriated amount and purity, and their stability was evaluated to determine the better conditions of storage, as well as shelf life. The isolation of all chromatographic standards was carried out with open column, quantification was done using spectrophotometry and purity evaluation was measured by HPLC. Many matrices have been evaluated as a source of standards, some new sources were found and showed better characteristics, as purity higher than 90%, if compared with those, frequently, used for this purpose. The study of stability showed that mixtures of carotenoids used as external standard for calibration can be storage more than 370 days. Validation study showed that this methodology of analysis has good selectivity, sensitivity and linearity in a concentration range from 0.5 to 8.0 ?g/mL for each carotenoid. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-03-12 |
dc.date.available.fl_str_mv |
2010-12-06 |
dc.date.accessioned.fl_str_mv |
2016-04-28T14:54:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PACHECO, Sidney. Preparo de padr?es anal?ticos, estudo de estabilidade e par?metros de valida??o para ensaio de caroten?ides por cromatografia l?quida. 2009. 109 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2009. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/tede/414 |
identifier_str_mv |
PACHECO, Sidney. Preparo de padr?es anal?ticos, estudo de estabilidade e par?metros de valida??o para ensaio de caroten?ides por cromatografia l?quida. 2009. 109 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2009. |
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https://tede.ufrrj.br/jspui/handle/tede/414 |
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Universidade Federal Rural do Rio de Janeiro |
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Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos |
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UFRRJ |
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Brasil |
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Instituto de Tecnologia |
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Universidade Federal Rural do Rio de Janeiro |
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