Influ?ncias e altera??es no perfil metab?lico de Alphitobius diaperinus (Coleoptera:Tenebrionidae) na sua utiliza??o como biodegradador de poliestireno
Ano de defesa: | 2022 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal Rural do Rio de Janeiro
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Biologia Animal
|
Departamento: |
Instituto de Ci?ncias Biol?gicas e da Sa?de
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://tede.ufrrj.br/jspui/handle/tede/589 |
Resumo: | The world production of plastics has increased very quickly in the last 50 years, with emphasis on the Chinese production, responsible for ? of the world production. To date, no environmentally friendly method is available to dispose of plastic waste, which accumulates in landfills or is scattered as microplastics. In this context, biodegradation emerges as a sustainable alternative, as it consists in the mineralization of these materials through submission to microbial metabolism. Current research has reported that some coleopteran species are able to degrade plastics with the aid of their chewing mouthparts and the metabolic activity of their intestinal microbiota. This study shows that Alphitobius diaperinus is able to feed on and apparently degrade polystyrene. The metabolic profile of larvae fed with poultry feed, subjected to absolute fasting and fed with polystyrene were characterized through a metabolic approach where the steps of the analysis of the metabolic profile involved sample preparation, extraction of substances from the tissue of the living organism, derivatization of extracted substances, injection into the chromatograph, processing and identification of substances, statistical analysis and interpretation of results. Along with this, the larval length, dry weight and integrity of the digestive tract were included. A total of 50 metabolites were found and among them the metabolites of the polyalcohol, nitrogen and amino acid classes in A. diaperinus larvae submitted to fasting and in the Polystyrene (PS) diet had a significant increase in relation to larvae fed with ration for birds. Showing that even if they were able to chew/crush polystyrene, the metabolism of larvae fed with PS is very similar to that of larvae in fasting condition. Despite this, this research supports the notion that while synthetic molecules, unlike most natural compounds, do not generally enter the natural food chain to be degraded by environmental microbiota, some species of alternative plastic degraders may be able to decompose plastics. Due to the results found, A diaperinus, although it does not decompose all the PS, can be used as its initial degrader. From this it appears that the present study will contribute in the long term to provide adequate, sustainable and profitable management of these post-consumer materials. |
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Pontes, Emerson Guedes986.393.587-53Souza, Marco Andre Alves dePontes, Emerson GuedesSantangelo, Jayme Magalh?esGrillo, Luciano Aparecido Meireles126.585.047-05http://lattes.cnpq.br/1430907238110348Hastenreiter, Luana da Silva Nascimento2022-06-20T14:59:47Z2022-02-24HASTENREITER, Luana da Silva Nascimento. Influ?ncias e altera??es no perfil metab?lico de Alphitobius diaperinus (Coleoptera:Tenebrionidae) na sua utiliza??o como biodegradador de poliestireno. 2022. 59 f. Disserta??o (Mestrado em Biologia Animal) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2022.https://tede.ufrrj.br/jspui/handle/tede/589The world production of plastics has increased very quickly in the last 50 years, with emphasis on the Chinese production, responsible for ? of the world production. To date, no environmentally friendly method is available to dispose of plastic waste, which accumulates in landfills or is scattered as microplastics. In this context, biodegradation emerges as a sustainable alternative, as it consists in the mineralization of these materials through submission to microbial metabolism. Current research has reported that some coleopteran species are able to degrade plastics with the aid of their chewing mouthparts and the metabolic activity of their intestinal microbiota. This study shows that Alphitobius diaperinus is able to feed on and apparently degrade polystyrene. The metabolic profile of larvae fed with poultry feed, subjected to absolute fasting and fed with polystyrene were characterized through a metabolic approach where the steps of the analysis of the metabolic profile involved sample preparation, extraction of substances from the tissue of the living organism, derivatization of extracted substances, injection into the chromatograph, processing and identification of substances, statistical analysis and interpretation of results. Along with this, the larval length, dry weight and integrity of the digestive tract were included. A total of 50 metabolites were found and among them the metabolites of the polyalcohol, nitrogen and amino acid classes in A. diaperinus larvae submitted to fasting and in the Polystyrene (PS) diet had a significant increase in relation to larvae fed with ration for birds. Showing that even if they were able to chew/crush polystyrene, the metabolism of larvae fed with PS is very similar to that of larvae in fasting condition. Despite this, this research supports the notion that while synthetic molecules, unlike most natural compounds, do not generally enter the natural food chain to be degraded by environmental microbiota, some species of alternative plastic degraders may be able to decompose plastics. Due to the results found, A diaperinus, although it does not decompose all the PS, can be used as its initial degrader. From this it appears that the present study will contribute in the long term to provide adequate, sustainable and profitable management of these post-consumer materials.A produ??o mundial de pl?sticos aumentou de forma bastante r?pida nos ?ltimos 50 anos, com destaque para a produ??o chinesa, respons?vel por ? da produ??o mundial. At? ao presente n?o est? dispon?vel um m?todo favor?vel ao meio ambiente para eliminar os res?duos pl?sticos, que se acumulam em aterros ou s?o espalhados como micropl?sticos. Nesse contexto, a biodegrada??o emerge como alternativa sustent?vel, pois consiste na mineraliza??o desses materiais mediante submiss?o ao metabolismo microbiano. Pesquisas atuais relataram que algumas esp?cies de cole?pteros s?o capazes de degradar pl?sticos com o aux?lio das suas pe?as bucais de mastiga??o e ? atividade metab?lica de sua microbiota intestinal. Este estudo mostra que o Alphitobius diaperinus ? capaz de se alimentar e aparentemente degradar o poliestireno. O perfil metab?lico de larvas alimentadas com ra??o para aves, submetidas a jejum absoluto e alimentas com poliestireno foram caracterizados atrav?s de uma abordagem metabolomica onde as etapas da an?lise do perfil metab?lico envolveram a prepara??o da amostra, extra??o das subst?ncias do tecido do organismo vivo, derivatiza??o das subst?ncias extra?das, inje??o no cromat?grafo, processamento e identifica??o das subst?ncias, an?lises estat?sticas e interpreta??o dos resultados. Junto a isso foi metido o comprimento da larva, peso seco e integridade do trato digestivo. Foram encontrados 57 metab?litos e dentre eles os metabolitos das classes dos poli?lcoois, nitrogenados e amino?cidos em larvas de A. diaperinus submetidas ao jejum e na dieta de Poliestireno (PS), tiveram um aumento significativo em rela??o as larvas alimentadas com a ra??o para aves. Mostrando que ainda que fossem capazes de mastigar/triturar o poliestireno, o metabolismo das larvas alimentadas com PS ? bem similar ao das larvas em condi??o de jejum. Apesar disso, esta pesquisa apoia a no??o de que, embora as mol?culas sint?ticas, ao contr?rio da maioria dos compostos naturais, geralmente n?o entrem na cadeia alimentar natural para serem degradadas pela microbiota ambiental, algumas esp?cies de degradadores de pl?sticos alternativos podem ser capazes de decompor pl?sticos. Em virtude dos resultados encontrados, o A diaperinus ainda que n?o decomponha todo o PS, pode ser utilizado como degradador inicial do mesmo. A partir disso depreende-se que o presente estudo contribuir? a longo prazo para fornecer um manejo adequado, sustent?vel e lucrativo a esses materiais p?s-consumo.Made available in DSpace on 2022-06-20T14:59:47Z (GMT). No. of bitstreams: 1 2022 - Luana da Silva Nascimento Hastenreiter.pdf:CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superiorapplication/pdfhttps://tede.ufrrj.br/retrieve/69747/2022%20-%20Luana%20da%20Silva%20Nascimento%20Hastenreiter.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Biologia AnimalUFRRJBrasilInstituto de Ci?ncias Biol?gicas e da Sa?debiodegrada??oisoporpl?sticoslarvacole?pterometabol?micabiodegradationstyrofoamplasticslarvaecoleopteranmetabolomicsBioqu?micaInflu?ncias e altera??es no perfil metab?lico de Alphitobius diaperinus (Coleoptera:Tenebrionidae) na sua utiliza??o como biodegradador de poliestirenoInfluences and changes in the metabolic profile of Alphitobius diaperinus (Coleoptera:Tenebrionidae) in its use as a polystyrene biodegraderinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2022 - Luana da Silva Nascimento Hastenreiter.pdf.jpg2022 - Luana da Silva Nascimento Hastenreiter.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/tede/589/20/2022+-+Luana+da+Silva+Nascimento+Hastenreiter.pdf.jpgcc73c4c239a4c332d642ba1e7c7a9fb2MD520TEXT2022 - Luana da Silva Nascimento Hastenreiter.pdf.txt2022 - Luana da Silva Nascimento Hastenreiter.pdf.txttext/plain135518http://localhost:8080/tede/bitstream/tede/589/19/2022+-+Luana+da+Silva+Nascimento+Hastenreiter.pdf.txtf692dda707e31b1bcb8f7d7c4b6984edMD519ORIGINAL2022 - Luana da Silva Nascimento Hastenreiter.pdf2022 - Luana da Silva Nascimento Hastenreiter.pdfapplication/pdf1614129http://localhost:8080/tede/bitstream/tede/589/18/2022+-+Luana+da+Silva+Nascimento+Hastenreiter.pdfba5c61dbbc2df20fa7a77a9b93b13a0cMD518tede/5892022-06-21 01:00:21.367oai:localhost:tede/589Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-06-21T04:00:21Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
dc.title.por.fl_str_mv |
Influ?ncias e altera??es no perfil metab?lico de Alphitobius diaperinus (Coleoptera:Tenebrionidae) na sua utiliza??o como biodegradador de poliestireno |
dc.title.alternative.eng.fl_str_mv |
Influences and changes in the metabolic profile of Alphitobius diaperinus (Coleoptera:Tenebrionidae) in its use as a polystyrene biodegrader |
title |
Influ?ncias e altera??es no perfil metab?lico de Alphitobius diaperinus (Coleoptera:Tenebrionidae) na sua utiliza??o como biodegradador de poliestireno |
spellingShingle |
Influ?ncias e altera??es no perfil metab?lico de Alphitobius diaperinus (Coleoptera:Tenebrionidae) na sua utiliza??o como biodegradador de poliestireno Hastenreiter, Luana da Silva Nascimento biodegrada??o isopor pl?sticos larva cole?ptero metabol?mica biodegradation styrofoam plastics larvae coleopteran metabolomics Bioqu?mica |
title_short |
Influ?ncias e altera??es no perfil metab?lico de Alphitobius diaperinus (Coleoptera:Tenebrionidae) na sua utiliza??o como biodegradador de poliestireno |
title_full |
Influ?ncias e altera??es no perfil metab?lico de Alphitobius diaperinus (Coleoptera:Tenebrionidae) na sua utiliza??o como biodegradador de poliestireno |
title_fullStr |
Influ?ncias e altera??es no perfil metab?lico de Alphitobius diaperinus (Coleoptera:Tenebrionidae) na sua utiliza??o como biodegradador de poliestireno |
title_full_unstemmed |
Influ?ncias e altera??es no perfil metab?lico de Alphitobius diaperinus (Coleoptera:Tenebrionidae) na sua utiliza??o como biodegradador de poliestireno |
title_sort |
Influ?ncias e altera??es no perfil metab?lico de Alphitobius diaperinus (Coleoptera:Tenebrionidae) na sua utiliza??o como biodegradador de poliestireno |
author |
Hastenreiter, Luana da Silva Nascimento |
author_facet |
Hastenreiter, Luana da Silva Nascimento |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Pontes, Emerson Guedes |
dc.contributor.advisor1ID.fl_str_mv |
986.393.587-53 |
dc.contributor.advisor-co1.fl_str_mv |
Souza, Marco Andre Alves de |
dc.contributor.referee1.fl_str_mv |
Pontes, Emerson Guedes |
dc.contributor.referee2.fl_str_mv |
Santangelo, Jayme Magalh?es |
dc.contributor.referee3.fl_str_mv |
Grillo, Luciano Aparecido Meireles |
dc.contributor.authorID.fl_str_mv |
126.585.047-05 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1430907238110348 |
dc.contributor.author.fl_str_mv |
Hastenreiter, Luana da Silva Nascimento |
contributor_str_mv |
Pontes, Emerson Guedes Souza, Marco Andre Alves de Pontes, Emerson Guedes Santangelo, Jayme Magalh?es Grillo, Luciano Aparecido Meireles |
dc.subject.por.fl_str_mv |
biodegrada??o isopor pl?sticos larva cole?ptero metabol?mica |
topic |
biodegrada??o isopor pl?sticos larva cole?ptero metabol?mica biodegradation styrofoam plastics larvae coleopteran metabolomics Bioqu?mica |
dc.subject.eng.fl_str_mv |
biodegradation styrofoam plastics larvae coleopteran metabolomics |
dc.subject.cnpq.fl_str_mv |
Bioqu?mica |
description |
The world production of plastics has increased very quickly in the last 50 years, with emphasis on the Chinese production, responsible for ? of the world production. To date, no environmentally friendly method is available to dispose of plastic waste, which accumulates in landfills or is scattered as microplastics. In this context, biodegradation emerges as a sustainable alternative, as it consists in the mineralization of these materials through submission to microbial metabolism. Current research has reported that some coleopteran species are able to degrade plastics with the aid of their chewing mouthparts and the metabolic activity of their intestinal microbiota. This study shows that Alphitobius diaperinus is able to feed on and apparently degrade polystyrene. The metabolic profile of larvae fed with poultry feed, subjected to absolute fasting and fed with polystyrene were characterized through a metabolic approach where the steps of the analysis of the metabolic profile involved sample preparation, extraction of substances from the tissue of the living organism, derivatization of extracted substances, injection into the chromatograph, processing and identification of substances, statistical analysis and interpretation of results. Along with this, the larval length, dry weight and integrity of the digestive tract were included. A total of 50 metabolites were found and among them the metabolites of the polyalcohol, nitrogen and amino acid classes in A. diaperinus larvae submitted to fasting and in the Polystyrene (PS) diet had a significant increase in relation to larvae fed with ration for birds. Showing that even if they were able to chew/crush polystyrene, the metabolism of larvae fed with PS is very similar to that of larvae in fasting condition. Despite this, this research supports the notion that while synthetic molecules, unlike most natural compounds, do not generally enter the natural food chain to be degraded by environmental microbiota, some species of alternative plastic degraders may be able to decompose plastics. Due to the results found, A diaperinus, although it does not decompose all the PS, can be used as its initial degrader. From this it appears that the present study will contribute in the long term to provide adequate, sustainable and profitable management of these post-consumer materials. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-06-20T14:59:47Z |
dc.date.issued.fl_str_mv |
2022-02-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
HASTENREITER, Luana da Silva Nascimento. Influ?ncias e altera??es no perfil metab?lico de Alphitobius diaperinus (Coleoptera:Tenebrionidae) na sua utiliza??o como biodegradador de poliestireno. 2022. 59 f. Disserta??o (Mestrado em Biologia Animal) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2022. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/tede/589 |
identifier_str_mv |
HASTENREITER, Luana da Silva Nascimento. Influ?ncias e altera??es no perfil metab?lico de Alphitobius diaperinus (Coleoptera:Tenebrionidae) na sua utiliza??o como biodegradador de poliestireno. 2022. 59 f. Disserta??o (Mestrado em Biologia Animal) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2022. |
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https://tede.ufrrj.br/jspui/handle/tede/589 |
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por |
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Universidade Federal Rural do Rio de Janeiro |
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Universidade Federal Rural do Rio de Janeiro |
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