Avalia??o da Estabilidade T?rmica de Histamina em Conservas de Pescado por CLAE

Detalhes bibliográficos
Ano de defesa: 2012
Autor(a) principal: Tavares, Rodrigo Domingos Overa lattes
Orientador(a): Furtado, Angela Aparecida Lemos lattes
Banca de defesa: Mathias, Simone Pereira, M?rsico, Eliane Teixeira
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal Rural do Rio de Janeiro
Programa de Pós-Graduação: Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos
Departamento: Instituto de Tecnologia
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: https://tede.ufrrj.br/jspui/handle/jspui/3624
Resumo: Histamine [4-(2-aminoethyl) imidazole] is a biogenic amine and nonvolatile synthesized by decarboxylation of free L-histidine. The presence of bacterial histidine decarboxylase, high temperatures and manipulation in inadequate sanitary conditions, are factors that enable the biosynthesis of histamine. Histamine has an important feature for its heat resistance, once synthesized, cannot be degraded through the process of sterilization, cooking, freezing, or any other type of treatment that may suffer from post-capture. This amine can be present in fish and fermented products (e.g. wine, beer, cheese, etc.) however, fish is the main food involved. Ingestion of high concentrations of histamine may cause the appearance of symptoms such as nausea, headache, diarrhea, itching and flushing, especially in susceptible individuals. It should be noted that fish with high levels of histamine, in most of cases do not have sensory changes but there are reports of development of spicy or metallic flavor. The main goal of this survey was to assess the effect of thermal processing on degradation of histamine in fish. High Performance Liquid Chromatography (HPLC) was used as a method of detection and quantification of histamine. The extraction methodology was performed using methanol and for the detection of analyte present in the aliquot of the vial was derivatized with the reagent 6-aminoquinolyl-hydroxysuccinimidyl-N-carbamate (AQC). The analyzed samples showed concentrations of histamine in the range 1.05 to 2.94mg kg-1, in other words below the current legislation, which corresponds to 100mg kg-1. There was a reduction in the concentration of histamine in comparison to fresh and canned fish. This fact can be explained by the solubility of histamine in covering liquid and dipping of fillets. The samples were also submitted to bacterial count of histidine decarboxylase. The values of 4.60 and 1.80 log CFU g-1 correspond to a sample of hybrid fish fillet and tuna, respectively. Monitoring the levels of histamine aims to ratify the food safety for consumers. In addition, preventive measures and corrective action recommended by the system of Hazard Analysis and Critical Control Points (HACCP) must be taken in the fishing industry to provide harmless food.
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spelling Furtado, Angela Aparecida Lemos878.390.327-53http://lattes.cnpq.br/7333993063180137Godoy, Ronoel Luiz de OliveiraMathias, Simone PereiraM?rsico, Eliane Teixeira112.447.857-41http://lattes.cnpq.br/3552053898977113Tavares, Rodrigo Domingos Overa2020-06-13T13:15:35Z2012-06-06TAVARES, Rodrigo Domingos Overa. Avalia??o da Estabilidade T?rmica de Histamina em Conservas de Pescado por CLAE. 2012. 52 f. Disserta??o (Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos) - Universidade Federal Rural do Rio de Janeiro, Serop?dica.https://tede.ufrrj.br/jspui/handle/jspui/3624Histamine [4-(2-aminoethyl) imidazole] is a biogenic amine and nonvolatile synthesized by decarboxylation of free L-histidine. The presence of bacterial histidine decarboxylase, high temperatures and manipulation in inadequate sanitary conditions, are factors that enable the biosynthesis of histamine. Histamine has an important feature for its heat resistance, once synthesized, cannot be degraded through the process of sterilization, cooking, freezing, or any other type of treatment that may suffer from post-capture. This amine can be present in fish and fermented products (e.g. wine, beer, cheese, etc.) however, fish is the main food involved. Ingestion of high concentrations of histamine may cause the appearance of symptoms such as nausea, headache, diarrhea, itching and flushing, especially in susceptible individuals. It should be noted that fish with high levels of histamine, in most of cases do not have sensory changes but there are reports of development of spicy or metallic flavor. The main goal of this survey was to assess the effect of thermal processing on degradation of histamine in fish. High Performance Liquid Chromatography (HPLC) was used as a method of detection and quantification of histamine. The extraction methodology was performed using methanol and for the detection of analyte present in the aliquot of the vial was derivatized with the reagent 6-aminoquinolyl-hydroxysuccinimidyl-N-carbamate (AQC). The analyzed samples showed concentrations of histamine in the range 1.05 to 2.94mg kg-1, in other words below the current legislation, which corresponds to 100mg kg-1. There was a reduction in the concentration of histamine in comparison to fresh and canned fish. This fact can be explained by the solubility of histamine in covering liquid and dipping of fillets. The samples were also submitted to bacterial count of histidine decarboxylase. The values of 4.60 and 1.80 log CFU g-1 correspond to a sample of hybrid fish fillet and tuna, respectively. Monitoring the levels of histamine aims to ratify the food safety for consumers. In addition, preventive measures and corrective action recommended by the system of Hazard Analysis and Critical Control Points (HACCP) must be taken in the fishing industry to provide harmless food.A histamina [4-(2-aminoetil) imidazol] ? uma amina biog?nica n?o-vol?til e sintetizada pela descarboxila??o do amino?cido L-histidina livre. A presen?a de bact?rias histidina descarboxilase, altas temperaturas e manipula??o em condi??es higi?nico-sanit?rias inadequadas, s?o fatores que viabilizam a bioss?ntese de histamina. A histamina tem como caracter?stica importante a sua termorresist?ncia pois, uma vez sintetizada, n?o se degrada atrav?s do processo de apertiza??o, cozimento, congelamento ou, qualquer outro tipo de tratamento nas etapas de p?s-captura. Essa amina pode estar presente no pescado e em produtos fermentados (e.g. vinho, cerveja, queijo, etc.) mas tem o pescado como principal alimento envolvido em epis?dios de intoxica??o. A ingest?o de altas concentra??es de histamina pode originar sintomas como n?useas, cefaleia, diarreia, prurido e rubor facial, principalmente em indiv?duos suscept?veis. Cabe destacar que os peixes com altos ?ndices de histamina, na maioria das vezes, n?o possuem altera??es sensoriais por?m, h? relatos do desenvolvimento de sabor picante ou met?lico no consumo. O objetivo deste trabalho foi avaliar o efeito do processamento t?rmico na degrada??o de histamina em pescado. A Cromatografia L?quida de Alta Efici?ncia (CLAE) foi utilizada como m?todo de detec??o e quantifica??o de histamina. A metodologia de extra??o consistiu em utilizar metanol e para a detec??o do analito, a al?quota presente em ?vial? foi derivatizada com o reagente 6-aminoquinolil-N-hydroxisuccinimidil carbamato (AQC). As amostras analisadas apresentaram concentra??es de histamina na faixa de 1,05 ? 2,94mg kg-1, ou seja, bem abaixo da legisla??o vigente, que corresponde a 100mg kg-1. Houve redu??o na concentra??o de histamina em compara??o ao fil? in natura e a conserva enlatada. Tal fato pode ser justificado pela solubilidade da histamina no l?quido de cobertura e de imers?o dos fil?s. As amostras tamb?m foram submetidas a contagem de bact?rias histidina descarboxilase. Os valores de 4,60 e 1,80 log UFC g-1 correspondem a contagem em amostra de fil? de pescado h?brido e de atum, respectivamente. O monitoramento dos teores de histamina visa ratificar a seguran?a alimentar para o consumidor. Al?m disso, medidas de a??o preventiva e corretiva preconizadas pelo sistema de An?lise de Perigos e Pontos Cr?ticos de Controle (APPCC) devem ser adotadas na ind?stria pesqueira a fim de fornecer alimentos in?cuos.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-06-13T13:15:35Z No. of bitstreams: 1 2012 - Rodrigo Domingos Overa Tavares.pdf: 1138214 bytes, checksum: a751b6b81f92a186b585ee5e006f3aae (MD5)Made available in DSpace on 2020-06-13T13:15:35Z (GMT). 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dc.title.por.fl_str_mv Avalia??o da Estabilidade T?rmica de Histamina em Conservas de Pescado por CLAE
dc.title.alternative.eng.fl_str_mv Assessment of Thermal Stability of Histamine in Canned Fish by HPLC
title Avalia??o da Estabilidade T?rmica de Histamina em Conservas de Pescado por CLAE
spellingShingle Avalia??o da Estabilidade T?rmica de Histamina em Conservas de Pescado por CLAE
Tavares, Rodrigo Domingos Overa
histamina
intoxica??o escombr?ide
pescado
histamine
scombroid poisoning
fish
Ci?ncias Agr?rias
title_short Avalia??o da Estabilidade T?rmica de Histamina em Conservas de Pescado por CLAE
title_full Avalia??o da Estabilidade T?rmica de Histamina em Conservas de Pescado por CLAE
title_fullStr Avalia??o da Estabilidade T?rmica de Histamina em Conservas de Pescado por CLAE
title_full_unstemmed Avalia??o da Estabilidade T?rmica de Histamina em Conservas de Pescado por CLAE
title_sort Avalia??o da Estabilidade T?rmica de Histamina em Conservas de Pescado por CLAE
author Tavares, Rodrigo Domingos Overa
author_facet Tavares, Rodrigo Domingos Overa
author_role author
dc.contributor.advisor1.fl_str_mv Furtado, Angela Aparecida Lemos
dc.contributor.advisor1ID.fl_str_mv 878.390.327-53
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/7333993063180137
dc.contributor.advisor-co1.fl_str_mv Godoy, Ronoel Luiz de Oliveira
dc.contributor.referee1.fl_str_mv Mathias, Simone Pereira
dc.contributor.referee2.fl_str_mv M?rsico, Eliane Teixeira
dc.contributor.authorID.fl_str_mv 112.447.857-41
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3552053898977113
dc.contributor.author.fl_str_mv Tavares, Rodrigo Domingos Overa
contributor_str_mv Furtado, Angela Aparecida Lemos
Godoy, Ronoel Luiz de Oliveira
Mathias, Simone Pereira
M?rsico, Eliane Teixeira
dc.subject.por.fl_str_mv histamina
intoxica??o escombr?ide
pescado
topic histamina
intoxica??o escombr?ide
pescado
histamine
scombroid poisoning
fish
Ci?ncias Agr?rias
dc.subject.eng.fl_str_mv histamine
scombroid poisoning
fish
dc.subject.cnpq.fl_str_mv Ci?ncias Agr?rias
description Histamine [4-(2-aminoethyl) imidazole] is a biogenic amine and nonvolatile synthesized by decarboxylation of free L-histidine. The presence of bacterial histidine decarboxylase, high temperatures and manipulation in inadequate sanitary conditions, are factors that enable the biosynthesis of histamine. Histamine has an important feature for its heat resistance, once synthesized, cannot be degraded through the process of sterilization, cooking, freezing, or any other type of treatment that may suffer from post-capture. This amine can be present in fish and fermented products (e.g. wine, beer, cheese, etc.) however, fish is the main food involved. Ingestion of high concentrations of histamine may cause the appearance of symptoms such as nausea, headache, diarrhea, itching and flushing, especially in susceptible individuals. It should be noted that fish with high levels of histamine, in most of cases do not have sensory changes but there are reports of development of spicy or metallic flavor. The main goal of this survey was to assess the effect of thermal processing on degradation of histamine in fish. High Performance Liquid Chromatography (HPLC) was used as a method of detection and quantification of histamine. The extraction methodology was performed using methanol and for the detection of analyte present in the aliquot of the vial was derivatized with the reagent 6-aminoquinolyl-hydroxysuccinimidyl-N-carbamate (AQC). The analyzed samples showed concentrations of histamine in the range 1.05 to 2.94mg kg-1, in other words below the current legislation, which corresponds to 100mg kg-1. There was a reduction in the concentration of histamine in comparison to fresh and canned fish. This fact can be explained by the solubility of histamine in covering liquid and dipping of fillets. The samples were also submitted to bacterial count of histidine decarboxylase. The values of 4.60 and 1.80 log CFU g-1 correspond to a sample of hybrid fish fillet and tuna, respectively. Monitoring the levels of histamine aims to ratify the food safety for consumers. In addition, preventive measures and corrective action recommended by the system of Hazard Analysis and Critical Control Points (HACCP) must be taken in the fishing industry to provide harmless food.
publishDate 2012
dc.date.issued.fl_str_mv 2012-06-06
dc.date.accessioned.fl_str_mv 2020-06-13T13:15:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv TAVARES, Rodrigo Domingos Overa. Avalia??o da Estabilidade T?rmica de Histamina em Conservas de Pescado por CLAE. 2012. 52 f. Disserta??o (Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos) - Universidade Federal Rural do Rio de Janeiro, Serop?dica.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/3624
identifier_str_mv TAVARES, Rodrigo Domingos Overa. Avalia??o da Estabilidade T?rmica de Histamina em Conservas de Pescado por CLAE. 2012. 52 f. Disserta??o (Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos) - Universidade Federal Rural do Rio de Janeiro, Serop?dica.
url https://tede.ufrrj.br/jspui/handle/jspui/3624
dc.language.iso.fl_str_mv por
language por
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