Presen?a de dinoflagelados bent?nicos potencialmente t?xicos: identifica??o do perigo em ?reas destinadas ? maricultura na Ba?a de Sepetiba, RJ
Ano de defesa: | 2009 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal Rural do Rio de Janeiro
|
Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos
|
Departamento: |
Instituto de Tecnologia
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://tede.ufrrj.br/jspui/handle/tede/431 |
Resumo: | The malacoculture (cultivation of shellfish) is the second most significant activity of the Brazilian mariculture, where the cultivation of bivalve molluscs (mussels, oysters and scallops) stands out nationally. The bivalves show an excellent nutritional value when they are used as food by humans, containing a great biological value of protein, low in fat, vitamins, minerals and carbohydrates. However, their contamination by toxic algae and its phycotoxins represents a danger to consumer s health, particularly in the production areas without monitoring, considered by the Intergovernmental Oceanographic Commission of high risk to public health. The effective monitoring is the only way to ensure the hygienic-sanitary quality of commercialized shellfish and consumer s safety. The diarrheic phycotoxin okadaic acid (OA) was detected in mussels from natural beds and from shellfish farms located in the bays of Sepetiba and Ilha Grande (RJ). Its production was associated with planktonic dinoflagellates of the Dinophysis genus. However, the role of benthic dinoflagellates in the production of OA and other benthic phycotoxins is still poorly studied. In this context, this study aimed to investigate the occurrence of potentially toxic benthic dinoflagellates associated with macroalgae collected in Guaiba and Marambaia islands, areas of cultivation and extraction of mussels (Perna perna). Macroalgae were collected monthly (january to december 2008) by snorkeling (1 meter), using a polyethylene bag, then were transferred to polyethylene bottles and agitated (1 minute) to separate the epiphytic dinoflagellates. The material was filtered on differents meshes (150, 115 and 20 ?m) and the final volume of 250ml was concentrated and set. Only the potentially toxic and harmful dinoflagellates were identified and quantified (Method of Utherm?l) under inverted microscope. The macroalgae were fixed and identified. The cell density of dinoflagellates was expressed on the dry weight of macroalgae (c?lulas.g-1). Were identified 32 species of macroalgae (8 Phaeophytes, 6 chlorophytes and 18 rhodophytes). From 97 samples of dinoflagellates was identified 11 morphotypes of dinoflagellates: Coolia sp., Prorocentrum cf. arenarium, P. cf. balticum, P. cf. concavum, P. cf. emarginatum, P. gracilis, P. cf. lima, P. cf. mexicanum, P. micans, Ostreopsis sp1 and sp2 occurring in Padina, Sargassum, Codium, Caulerpa and Laurence, substrates frequently during the study period. The analysis of variance bifactorial showed differences between spatial and temporal concentrations of Ostreopsis and Prorocentrum. Ostreopsis occurred throughout the year on both sites associated mainly with Padina gymnospora. The Guaiba island constantly showed higher cell densities and richness of Prorocentrum. Summer was the season of year when Prorocentrum was more abundant. Coolia showed punctual and sporadic occurrence. The knowledge of the occurrence of potentially toxic dinoflagellates is important to better understand the real implications of these species in the production and transference of toxins in coastal trophic web, and can help generating information to the development of mariculture in the southern coast of Rio de Janeiro from the perspective of safe food. |
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Silva, Pedro Paulo de Oliveira709.955.917-68http://lattes.cnpq.br/4059934620802246025.777.537-48http://lattes.cnpq.br/8045925494960939Oliveira, Gesilene Mendon?a de2016-04-28T14:55:28Z2009-08-282009-07-10OLIVEIRA, Gesilene Mendon?a de. Presen?a de dinoflagelados bent?nicos potencialmente t?xicos: identifica??o do perigo em ?reas destinadas ? maricultura na Ba?a de Sepetiba, RJ. 2009. 129 f. Tese (Doutorado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2009.https://tede.ufrrj.br/jspui/handle/tede/431The malacoculture (cultivation of shellfish) is the second most significant activity of the Brazilian mariculture, where the cultivation of bivalve molluscs (mussels, oysters and scallops) stands out nationally. The bivalves show an excellent nutritional value when they are used as food by humans, containing a great biological value of protein, low in fat, vitamins, minerals and carbohydrates. However, their contamination by toxic algae and its phycotoxins represents a danger to consumer s health, particularly in the production areas without monitoring, considered by the Intergovernmental Oceanographic Commission of high risk to public health. The effective monitoring is the only way to ensure the hygienic-sanitary quality of commercialized shellfish and consumer s safety. The diarrheic phycotoxin okadaic acid (OA) was detected in mussels from natural beds and from shellfish farms located in the bays of Sepetiba and Ilha Grande (RJ). Its production was associated with planktonic dinoflagellates of the Dinophysis genus. However, the role of benthic dinoflagellates in the production of OA and other benthic phycotoxins is still poorly studied. In this context, this study aimed to investigate the occurrence of potentially toxic benthic dinoflagellates associated with macroalgae collected in Guaiba and Marambaia islands, areas of cultivation and extraction of mussels (Perna perna). Macroalgae were collected monthly (january to december 2008) by snorkeling (1 meter), using a polyethylene bag, then were transferred to polyethylene bottles and agitated (1 minute) to separate the epiphytic dinoflagellates. The material was filtered on differents meshes (150, 115 and 20 ?m) and the final volume of 250ml was concentrated and set. Only the potentially toxic and harmful dinoflagellates were identified and quantified (Method of Utherm?l) under inverted microscope. The macroalgae were fixed and identified. The cell density of dinoflagellates was expressed on the dry weight of macroalgae (c?lulas.g-1). Were identified 32 species of macroalgae (8 Phaeophytes, 6 chlorophytes and 18 rhodophytes). From 97 samples of dinoflagellates was identified 11 morphotypes of dinoflagellates: Coolia sp., Prorocentrum cf. arenarium, P. cf. balticum, P. cf. concavum, P. cf. emarginatum, P. gracilis, P. cf. lima, P. cf. mexicanum, P. micans, Ostreopsis sp1 and sp2 occurring in Padina, Sargassum, Codium, Caulerpa and Laurence, substrates frequently during the study period. The analysis of variance bifactorial showed differences between spatial and temporal concentrations of Ostreopsis and Prorocentrum. Ostreopsis occurred throughout the year on both sites associated mainly with Padina gymnospora. The Guaiba island constantly showed higher cell densities and richness of Prorocentrum. Summer was the season of year when Prorocentrum was more abundant. Coolia showed punctual and sporadic occurrence. The knowledge of the occurrence of potentially toxic dinoflagellates is important to better understand the real implications of these species in the production and transference of toxins in coastal trophic web, and can help generating information to the development of mariculture in the southern coast of Rio de Janeiro from the perspective of safe food.A malacocultura (cultivo de moluscos) ? a segunda atividade mais expressiva da maricultura brasileira, onde o cultivo de moluscos bivalvos (mexilh?es, ostras e vieiras) destaca-se nacionalmente. Como alimento utilizado pelo homem, os bivalvos s?o considerados de excelente valor nutricional, pois cont?m prote?nas de excelente valor biol?gico, baixo teor de gorduras, vitaminas, minerais e carboidratos. No entanto, a contamina??o destes organismos por algas t?xicas e pelas ficotoxinas constitui um perigo para a sa?de dos consumidores, principalmente nas ?reas de produ??o sem monitoramento, consideradas pela Comiss?o Oceanogr?fica Intergovernamental de alto risco para a sa?de p?blica. O monitoramento efetivo ? a ?nica forma de garantir a qualidade higi?nico-sanit?ria do molusco comercializado e a seguran?a do consumidor. Nas Ba?as de Sepetiba e Ilha Grande (RJ) a ficotoxina diarr?ica ?cido okadaico (AO) foi detectada em mexilh?es de bancos naturais e cultivados. Sua produ??o foi associada a dinoflagelados planct?nicos do g?nero Dinophysis. No entanto, o papel de dinoflagelados bent?nicos na produ??o de AO e de outras ficotoxinas de origem bent?nica ainda ? pouco estudado. Neste contexto, o presente estudo objetivou investigar a ocorr?ncia de dinoflagelados bent?nicos potencialmente t?xicos associados ? macroalgas coletadas nas ilhas Gua?ba e Marambaia por serem ?reas de cultivo e de extra??o natural de mexilh?es (Perna perna). Macroalgas foram coletadas mensalmente (janeiro a dezembro de 2008) por mergulho livre (1 metro), usando bolsa de polietileno. Em seguida foram transferidas para garrafas de polietileno e agitadas (1 minuto) para separar os dinoflagelados ep?fitos. O material foi filtrado em diferentes malhas (150, 115 e 20?m) e o volume final de 250ml foi concentrado e fixado. Somente os dinoflagelados potencialmente t?xicos e nocivos foram identificados e quantificados (M?todo de Utherm?l) sob microscopia invertida. As macroalgas foram fixadas e identificadas. A densidade celular de dinoflagelados foi expressa em rela??o ao peso seco da macroalga (c?lulas.g-1). Foram identificadas 32 esp?cies de macroalgas (8 feof?ceas, 6 clorof?ceas e 18 rodof?ceas). A partir de 97 amostras de dinoflagelados identificou-se 11 morfotipos de dinoflagelados: Coolia sp., Prorocentrum cf. arenarium, P. cf. balticum, P. cf. concavum, P. cf. emarginatum, P. gracile, P. cf. lima, P. cf. mexicanum, P. micans, Ostreopsis sp1 e sp2 ocorrendo em Padina, Sargassum, Codium, Caulerpa e Laur?ncia, substratos mais freq?entes no per?odo estudado. A an?lise de vari?ncia bifatorial mostrou diferen?as espaciais e temporais entre as concentra??es de Ostreopsis e Prorocentrum. Ostreopsis ocorreu ao longo de todo o ano, em ambos locais associados principalmente ? Padina gymnospora. A ilha Gua?ba apresentou constantemente maiores densidades celulares e riqueza de Prorocentrum. O ver?o foi ? ?poca do ano onde Prorocentrum foi mais abundante. Coolia apresentou ocorr?ncia pontual e espor?dica. O conhecimento da ocorr?ncia de dinoflagelados potencialmente t?xicos ? importante para melhor compreender a real implica??o dessas esp?cies na produ??o e transfer?ncia de toxinas na teia tr?fica costeira, al?m de poder contribuir, com as informa??es geradas, para o desenvolvimento da maricultura respons?vel no litoral sul do Rio de Janeiro sob a perspectiva do alimento seguro.Made available in DSpace on 2016-04-28T14:55:28Z (GMT). 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dc.title.por.fl_str_mv |
Presen?a de dinoflagelados bent?nicos potencialmente t?xicos: identifica??o do perigo em ?reas destinadas ? maricultura na Ba?a de Sepetiba, RJ |
dc.title.alternative.eng.fl_str_mv |
Presence of potentially toxic benthic dinoflagellates: identification of hazard in shellfish growing areas in the Sepetiba Bay, RJ |
title |
Presen?a de dinoflagelados bent?nicos potencialmente t?xicos: identifica??o do perigo em ?reas destinadas ? maricultura na Ba?a de Sepetiba, RJ |
spellingShingle |
Presen?a de dinoflagelados bent?nicos potencialmente t?xicos: identifica??o do perigo em ?reas destinadas ? maricultura na Ba?a de Sepetiba, RJ Oliveira, Gesilene Mendon?a de mussels phycotoxins safe food mexilh?es ficotoxinas alimento seguro Ci?ncia e Tecnologia de Alimentos |
title_short |
Presen?a de dinoflagelados bent?nicos potencialmente t?xicos: identifica??o do perigo em ?reas destinadas ? maricultura na Ba?a de Sepetiba, RJ |
title_full |
Presen?a de dinoflagelados bent?nicos potencialmente t?xicos: identifica??o do perigo em ?reas destinadas ? maricultura na Ba?a de Sepetiba, RJ |
title_fullStr |
Presen?a de dinoflagelados bent?nicos potencialmente t?xicos: identifica??o do perigo em ?reas destinadas ? maricultura na Ba?a de Sepetiba, RJ |
title_full_unstemmed |
Presen?a de dinoflagelados bent?nicos potencialmente t?xicos: identifica??o do perigo em ?reas destinadas ? maricultura na Ba?a de Sepetiba, RJ |
title_sort |
Presen?a de dinoflagelados bent?nicos potencialmente t?xicos: identifica??o do perigo em ?reas destinadas ? maricultura na Ba?a de Sepetiba, RJ |
author |
Oliveira, Gesilene Mendon?a de |
author_facet |
Oliveira, Gesilene Mendon?a de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Pedro Paulo de Oliveira |
dc.contributor.advisor1ID.fl_str_mv |
709.955.917-68 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4059934620802246 |
dc.contributor.authorID.fl_str_mv |
025.777.537-48 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8045925494960939 |
dc.contributor.author.fl_str_mv |
Oliveira, Gesilene Mendon?a de |
contributor_str_mv |
Silva, Pedro Paulo de Oliveira |
dc.subject.eng.fl_str_mv |
mussels phycotoxins safe food |
topic |
mussels phycotoxins safe food mexilh?es ficotoxinas alimento seguro Ci?ncia e Tecnologia de Alimentos |
dc.subject.por.fl_str_mv |
mexilh?es ficotoxinas alimento seguro |
dc.subject.cnpq.fl_str_mv |
Ci?ncia e Tecnologia de Alimentos |
description |
The malacoculture (cultivation of shellfish) is the second most significant activity of the Brazilian mariculture, where the cultivation of bivalve molluscs (mussels, oysters and scallops) stands out nationally. The bivalves show an excellent nutritional value when they are used as food by humans, containing a great biological value of protein, low in fat, vitamins, minerals and carbohydrates. However, their contamination by toxic algae and its phycotoxins represents a danger to consumer s health, particularly in the production areas without monitoring, considered by the Intergovernmental Oceanographic Commission of high risk to public health. The effective monitoring is the only way to ensure the hygienic-sanitary quality of commercialized shellfish and consumer s safety. The diarrheic phycotoxin okadaic acid (OA) was detected in mussels from natural beds and from shellfish farms located in the bays of Sepetiba and Ilha Grande (RJ). Its production was associated with planktonic dinoflagellates of the Dinophysis genus. However, the role of benthic dinoflagellates in the production of OA and other benthic phycotoxins is still poorly studied. In this context, this study aimed to investigate the occurrence of potentially toxic benthic dinoflagellates associated with macroalgae collected in Guaiba and Marambaia islands, areas of cultivation and extraction of mussels (Perna perna). Macroalgae were collected monthly (january to december 2008) by snorkeling (1 meter), using a polyethylene bag, then were transferred to polyethylene bottles and agitated (1 minute) to separate the epiphytic dinoflagellates. The material was filtered on differents meshes (150, 115 and 20 ?m) and the final volume of 250ml was concentrated and set. Only the potentially toxic and harmful dinoflagellates were identified and quantified (Method of Utherm?l) under inverted microscope. The macroalgae were fixed and identified. The cell density of dinoflagellates was expressed on the dry weight of macroalgae (c?lulas.g-1). Were identified 32 species of macroalgae (8 Phaeophytes, 6 chlorophytes and 18 rhodophytes). From 97 samples of dinoflagellates was identified 11 morphotypes of dinoflagellates: Coolia sp., Prorocentrum cf. arenarium, P. cf. balticum, P. cf. concavum, P. cf. emarginatum, P. gracilis, P. cf. lima, P. cf. mexicanum, P. micans, Ostreopsis sp1 and sp2 occurring in Padina, Sargassum, Codium, Caulerpa and Laurence, substrates frequently during the study period. The analysis of variance bifactorial showed differences between spatial and temporal concentrations of Ostreopsis and Prorocentrum. Ostreopsis occurred throughout the year on both sites associated mainly with Padina gymnospora. The Guaiba island constantly showed higher cell densities and richness of Prorocentrum. Summer was the season of year when Prorocentrum was more abundant. Coolia showed punctual and sporadic occurrence. The knowledge of the occurrence of potentially toxic dinoflagellates is important to better understand the real implications of these species in the production and transference of toxins in coastal trophic web, and can help generating information to the development of mariculture in the southern coast of Rio de Janeiro from the perspective of safe food. |
publishDate |
2009 |
dc.date.available.fl_str_mv |
2009-08-28 |
dc.date.issued.fl_str_mv |
2009-07-10 |
dc.date.accessioned.fl_str_mv |
2016-04-28T14:55:28Z |
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OLIVEIRA, Gesilene Mendon?a de. Presen?a de dinoflagelados bent?nicos potencialmente t?xicos: identifica??o do perigo em ?reas destinadas ? maricultura na Ba?a de Sepetiba, RJ. 2009. 129 f. Tese (Doutorado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2009. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/tede/431 |
identifier_str_mv |
OLIVEIRA, Gesilene Mendon?a de. Presen?a de dinoflagelados bent?nicos potencialmente t?xicos: identifica??o do perigo em ?reas destinadas ? maricultura na Ba?a de Sepetiba, RJ. 2009. 129 f. Tese (Doutorado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2009. |
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https://tede.ufrrj.br/jspui/handle/tede/431 |
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Universidade Federal Rural do Rio de Janeiro |
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Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos |
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