Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos

Detalhes bibliográficos
Ano de defesa: 2015
Autor(a) principal: Pereira, Joana de Novais lattes
Orientador(a): Godoy, Ronoel Luiz de Oliveira lattes
Banca de defesa: Galdeano, Melicia Cintia, Carvalho, Carlos Wanderlei Piler de
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal Rural do Rio de Janeiro
Programa de Pós-Graduação: Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos
Departamento: Instituto de Tecnologia
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: https://tede.ufrrj.br/jspui/handle/jspui/4191
Resumo: The soybean with black coat (Glycine max (L.) Merrill) is attracting great interest to consumers and researchers because its consumption is associated with benefits to human health, in part associated with the presence of secondary metabolites, mainly isoflavones and anthocyanins. Embrapa has different strains of black soybeans that need to be assessed not only in relation to their agronomic characteristics, but also suitable for human consumption. Therefore, this study aimed to evaluate and compare four different strains of black soybean developed by Embrapa as the chemical composition, antinutritional factors, content and profile of isoflavones, anthocyanins, amino acids, carotenoids and antioxidant activity measured by DPPH method and ABTS. In respect to composition, there was no statistical difference (p> 0.05) between the protein (41.15 to 41.69 g/100) and moisture (from 10.41 to 10.61 g/100 g) in four soybean lines, while the ether extract values (19.12 to 19.79 g/100 g), carbohydrates (33.38 to 34.22 g / 100g) and ash (5.13 to 5.81 g / 100g ) varied statistically. The BRM11-51400 was the lineage with the most significant result for mineral as it showed the highest potassium content (2372.89 mg / 100g), most abundant mineral in all samples, phosphorus (753.56 mg / 100g ), magnesium (255.32 mg / 100 g) and calcium (257.82 mg / 100). It was also determined the concentration of manganese, zinc, copper, cobalt, selenium, iron and aluminum in significant amounts (9.04 to 11.52 mg / 100g). The higher sucrose content (6.46 g / 100 g) and raffinose (1.56g / 100g) was observed in BRM90-50901 while BRM11-51400 was the strain with the highest concentration of stachyose (5.24g /100g). Phytate varied from 11.91 to 14.88 mg / 100g, respectively. In relation to bioactive compounds, the BRM09-50995 lineage showed the highest levels of total monomeric anthocyanins, total isoflavones and carotenoids. In all three soybean linages monomeric anthocyanins were identified: cyanidin-3-O-glucoside, majority profile in all samples (262.27 to 720.45 mg / 100g), delphinidin-3-O-glucoside (184.41 to 316.72 mg / 100g) and petunidin-3-O-glucoside (6.34 to 153.06 mg / 100g). The isoflavones daidzein, glycitin, genistein were also identified at higher levels, and their aglycone forms, daidzein, glycitein and genistein. It was not found statistically significant difference between the total isoflavone content in BRM09-50995 linage (138.65 mg / 100 g) and BRM11-51400 (140.75 mg / 100 g), which had the highest content of this compound. The greater the DPPH antioxidant activity was observed in BRM09-50995 (1072.92 g / g DPPH), while BRM11-51400 showed the most significant results when used in the method of ABTS radical. Based on the results obtained in this study, it was found that the four soybean lineages are suitable for human consumption.
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spelling Godoy, Ronoel Luiz de Oliveira507.802.047-00http://lattes.cnpq.br/3671854931659782Felberg, IlanaGaldeano, Melicia CintiaCarvalho, Carlos Wanderlei Piler de121.994.117-45http://lattes.cnpq.br/0282669959921778Pereira, Joana de Novais2020-11-24T17:24:02Z2015-02-02PEREIRA, Joana de Novais. Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos. 2015. 73 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2015.https://tede.ufrrj.br/jspui/handle/jspui/4191The soybean with black coat (Glycine max (L.) Merrill) is attracting great interest to consumers and researchers because its consumption is associated with benefits to human health, in part associated with the presence of secondary metabolites, mainly isoflavones and anthocyanins. Embrapa has different strains of black soybeans that need to be assessed not only in relation to their agronomic characteristics, but also suitable for human consumption. Therefore, this study aimed to evaluate and compare four different strains of black soybean developed by Embrapa as the chemical composition, antinutritional factors, content and profile of isoflavones, anthocyanins, amino acids, carotenoids and antioxidant activity measured by DPPH method and ABTS. In respect to composition, there was no statistical difference (p> 0.05) between the protein (41.15 to 41.69 g/100) and moisture (from 10.41 to 10.61 g/100 g) in four soybean lines, while the ether extract values (19.12 to 19.79 g/100 g), carbohydrates (33.38 to 34.22 g / 100g) and ash (5.13 to 5.81 g / 100g ) varied statistically. The BRM11-51400 was the lineage with the most significant result for mineral as it showed the highest potassium content (2372.89 mg / 100g), most abundant mineral in all samples, phosphorus (753.56 mg / 100g ), magnesium (255.32 mg / 100 g) and calcium (257.82 mg / 100). It was also determined the concentration of manganese, zinc, copper, cobalt, selenium, iron and aluminum in significant amounts (9.04 to 11.52 mg / 100g). The higher sucrose content (6.46 g / 100 g) and raffinose (1.56g / 100g) was observed in BRM90-50901 while BRM11-51400 was the strain with the highest concentration of stachyose (5.24g /100g). Phytate varied from 11.91 to 14.88 mg / 100g, respectively. In relation to bioactive compounds, the BRM09-50995 lineage showed the highest levels of total monomeric anthocyanins, total isoflavones and carotenoids. In all three soybean linages monomeric anthocyanins were identified: cyanidin-3-O-glucoside, majority profile in all samples (262.27 to 720.45 mg / 100g), delphinidin-3-O-glucoside (184.41 to 316.72 mg / 100g) and petunidin-3-O-glucoside (6.34 to 153.06 mg / 100g). The isoflavones daidzein, glycitin, genistein were also identified at higher levels, and their aglycone forms, daidzein, glycitein and genistein. It was not found statistically significant difference between the total isoflavone content in BRM09-50995 linage (138.65 mg / 100 g) and BRM11-51400 (140.75 mg / 100 g), which had the highest content of this compound. The greater the DPPH antioxidant activity was observed in BRM09-50995 (1072.92 g / g DPPH), while BRM11-51400 showed the most significant results when used in the method of ABTS radical. Based on the results obtained in this study, it was found that the four soybean lineages are suitable for human consumption.O gr?o de soja com tegumento preto (Glycine max (L.) Merrill) vem despertando interesse de consumidores e pesquisadores devido o seu consumo estar associado com benef?cios ? sa?de humana, relacionados ? presen?a de metab?litos secund?rios, isoflavonas e principalmente das antocianinas. A Embrapa disp?e de diferentes linhagens de soja preta que precisam ser avaliadas n?o somente em rela??o ?s suas caracter?sticas agron?micas, mas se s?o adequadas ao consumo humano. O presente trabalho teve como objetivo geral avaliar e comparar quatro diferentes linhagens de soja preta desenvolvidas pela Embrapa quanto ? composi??o centesimal, fatores antinutricionais, teores de a??cares, perfil e teores de isoflavonas, antocianinas, amino?cidos, carotenoides, al?m da atividade antioxidante medida pelo m?todo DPPH e ABTS. Em rela??o ? composi??o centesimal, n?o foi observada diferen?a estat?stica entre os teores de prote?na (41,15 a 41,69 g/100) e umidade (10,41 a 10,61 g/100g) nas quatro linhagens de soja, enquanto os valores de extrato et?reo (19,12 a 19,79 g/100g), cinzas (5,13 a 5,81 g/100g) e carboidratos (33,38 a 34,22 g/100g) variaram estatisticamente (p?0,05). A BRM11-51400 foi a linhagem com o resultado mais expressivo para minerais, uma vez que apresentou o maior teor de pot?ssio (2372,89 mg/100g), mineral mais abundante em todas as amostras avaliadas, f?sforo (753,56 mg/100g), magn?sio (255,32 mg/100g) e c?lcio (257,82 mg/100). Foi tamb?m determinada a concentra??o de mangan?s, zinco, cobre, cobalto, sel?nio, alum?nio e ferro em teores significativos (9,04 a 11,52 mg/100g). O maior teor de sacarose (6,46 g/100g) e de rafinose (1,56 g/100g) foi observado na BRM09-50901, enquanto a BRM11-51400 foi a linhagem com a maior concentra??o de estaquiose (5,24 g/100g). O fitato ? um fator antinutricional presente em gr?o de soja, e foi detectado em teores que variaram de 11,91 a 14,88 mg/100g. Em rela??o aos compostos bioativos, a linhagem BRM09-50995, apresentou os teores mais elevados de antocianinas monom?ricas totais, isoflavonas totais e carotenoides totais. Foram identificadas nas linhagens de soja tr?s antocianinas monom?ricas: a cianidina-3-O-glicos?deo, perfil majorit?rio em todas as amostras (262,27 a 720,45 mg/100g), a delfinidina-3-O-glicos?deo (184,41 a 316,72 mg/100g) e a petunidina-3-O-glicos?deo (6,34 a 153,06 mg/100g). Foram tamb?m identificadas as isoflavonas: daidzina, glicitina e a genistina em maiores concentra??es, e suas respectivas formas agliconas, daidze?na, glicite?na e geniste?na. N?o foi observada diferen?a estat?stica entre os teores de isoflavonas totais nas linhagens BRM09-50995 (138,65 mg/100g) e BRM11-51400 (140,75 mg/100g), que apresentaram o maior teor deste composto. A maior capacidade antioxidante pelo m?todo DPPH foi observada na linhagem BRM09-50995 (1072,92 g/g DPPH), enquanto a BRM11-51400 apresentou o resultado mais expressivo quando utilizado o m?todo do radical ABTS. Embora a linhagem BRM09-50995 tenha apresentando os maiores teores de compostos bioativos, com base nos resultados obtidos nesse estudo, verificou-se que as quatro linhagens de soja s?o adequadas ao consumo humano.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-11-24T17:24:02Z No. of bitstreams: 1 2015 - Joana de Novais Pereira.pdf: 2767080 bytes, checksum: 04a4881c3f47e48b043ba5d458e72c67 (MD5)Made available in DSpace on 2020-11-24T17:24:02Z (GMT). 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dc.title.por.fl_str_mv Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos
dc.title.alternative.eng.fl_str_mv Evaluation of different lines of black soybeans as theirs physical and chemical characteristics, nutritional and bioactive compounds
title Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos
spellingShingle Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos
Pereira, Joana de Novais
Glycine max
composi??o centesimal
compostos fen?licos
atividade antioxidante
Glycine max
chemical composition
phenolic compounds
antioxidant activity
Ci?ncia e Tecnologia de Alimentos
title_short Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos
title_full Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos
title_fullStr Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos
title_full_unstemmed Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos
title_sort Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos
author Pereira, Joana de Novais
author_facet Pereira, Joana de Novais
author_role author
dc.contributor.advisor1.fl_str_mv Godoy, Ronoel Luiz de Oliveira
dc.contributor.advisor1ID.fl_str_mv 507.802.047-00
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3671854931659782
dc.contributor.advisor-co1.fl_str_mv Felberg, Ilana
dc.contributor.referee1.fl_str_mv Galdeano, Melicia Cintia
dc.contributor.referee2.fl_str_mv Carvalho, Carlos Wanderlei Piler de
dc.contributor.authorID.fl_str_mv 121.994.117-45
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0282669959921778
dc.contributor.author.fl_str_mv Pereira, Joana de Novais
contributor_str_mv Godoy, Ronoel Luiz de Oliveira
Felberg, Ilana
Galdeano, Melicia Cintia
Carvalho, Carlos Wanderlei Piler de
dc.subject.por.fl_str_mv Glycine max
composi??o centesimal
compostos fen?licos
atividade antioxidante
topic Glycine max
composi??o centesimal
compostos fen?licos
atividade antioxidante
Glycine max
chemical composition
phenolic compounds
antioxidant activity
Ci?ncia e Tecnologia de Alimentos
dc.subject.eng.fl_str_mv Glycine max
chemical composition
phenolic compounds
antioxidant activity
dc.subject.cnpq.fl_str_mv Ci?ncia e Tecnologia de Alimentos
description The soybean with black coat (Glycine max (L.) Merrill) is attracting great interest to consumers and researchers because its consumption is associated with benefits to human health, in part associated with the presence of secondary metabolites, mainly isoflavones and anthocyanins. Embrapa has different strains of black soybeans that need to be assessed not only in relation to their agronomic characteristics, but also suitable for human consumption. Therefore, this study aimed to evaluate and compare four different strains of black soybean developed by Embrapa as the chemical composition, antinutritional factors, content and profile of isoflavones, anthocyanins, amino acids, carotenoids and antioxidant activity measured by DPPH method and ABTS. In respect to composition, there was no statistical difference (p> 0.05) between the protein (41.15 to 41.69 g/100) and moisture (from 10.41 to 10.61 g/100 g) in four soybean lines, while the ether extract values (19.12 to 19.79 g/100 g), carbohydrates (33.38 to 34.22 g / 100g) and ash (5.13 to 5.81 g / 100g ) varied statistically. The BRM11-51400 was the lineage with the most significant result for mineral as it showed the highest potassium content (2372.89 mg / 100g), most abundant mineral in all samples, phosphorus (753.56 mg / 100g ), magnesium (255.32 mg / 100 g) and calcium (257.82 mg / 100). It was also determined the concentration of manganese, zinc, copper, cobalt, selenium, iron and aluminum in significant amounts (9.04 to 11.52 mg / 100g). The higher sucrose content (6.46 g / 100 g) and raffinose (1.56g / 100g) was observed in BRM90-50901 while BRM11-51400 was the strain with the highest concentration of stachyose (5.24g /100g). Phytate varied from 11.91 to 14.88 mg / 100g, respectively. In relation to bioactive compounds, the BRM09-50995 lineage showed the highest levels of total monomeric anthocyanins, total isoflavones and carotenoids. In all three soybean linages monomeric anthocyanins were identified: cyanidin-3-O-glucoside, majority profile in all samples (262.27 to 720.45 mg / 100g), delphinidin-3-O-glucoside (184.41 to 316.72 mg / 100g) and petunidin-3-O-glucoside (6.34 to 153.06 mg / 100g). The isoflavones daidzein, glycitin, genistein were also identified at higher levels, and their aglycone forms, daidzein, glycitein and genistein. It was not found statistically significant difference between the total isoflavone content in BRM09-50995 linage (138.65 mg / 100 g) and BRM11-51400 (140.75 mg / 100 g), which had the highest content of this compound. The greater the DPPH antioxidant activity was observed in BRM09-50995 (1072.92 g / g DPPH), while BRM11-51400 showed the most significant results when used in the method of ABTS radical. Based on the results obtained in this study, it was found that the four soybean lineages are suitable for human consumption.
publishDate 2015
dc.date.issued.fl_str_mv 2015-02-02
dc.date.accessioned.fl_str_mv 2020-11-24T17:24:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv PEREIRA, Joana de Novais. Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos. 2015. 73 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2015.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/4191
identifier_str_mv PEREIRA, Joana de Novais. Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos. 2015. 73 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2015.
url https://tede.ufrrj.br/jspui/handle/jspui/4191
dc.language.iso.fl_str_mv por
language por
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