Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos
Ano de defesa: | 2015 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal Rural do Rio de Janeiro
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Programa de Pós-Graduação: |
Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos
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Departamento: |
Instituto de Tecnologia
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País: |
Brasil
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Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://tede.ufrrj.br/jspui/handle/jspui/4191 |
Resumo: | The soybean with black coat (Glycine max (L.) Merrill) is attracting great interest to consumers and researchers because its consumption is associated with benefits to human health, in part associated with the presence of secondary metabolites, mainly isoflavones and anthocyanins. Embrapa has different strains of black soybeans that need to be assessed not only in relation to their agronomic characteristics, but also suitable for human consumption. Therefore, this study aimed to evaluate and compare four different strains of black soybean developed by Embrapa as the chemical composition, antinutritional factors, content and profile of isoflavones, anthocyanins, amino acids, carotenoids and antioxidant activity measured by DPPH method and ABTS. In respect to composition, there was no statistical difference (p> 0.05) between the protein (41.15 to 41.69 g/100) and moisture (from 10.41 to 10.61 g/100 g) in four soybean lines, while the ether extract values (19.12 to 19.79 g/100 g), carbohydrates (33.38 to 34.22 g / 100g) and ash (5.13 to 5.81 g / 100g ) varied statistically. The BRM11-51400 was the lineage with the most significant result for mineral as it showed the highest potassium content (2372.89 mg / 100g), most abundant mineral in all samples, phosphorus (753.56 mg / 100g ), magnesium (255.32 mg / 100 g) and calcium (257.82 mg / 100). It was also determined the concentration of manganese, zinc, copper, cobalt, selenium, iron and aluminum in significant amounts (9.04 to 11.52 mg / 100g). The higher sucrose content (6.46 g / 100 g) and raffinose (1.56g / 100g) was observed in BRM90-50901 while BRM11-51400 was the strain with the highest concentration of stachyose (5.24g /100g). Phytate varied from 11.91 to 14.88 mg / 100g, respectively. In relation to bioactive compounds, the BRM09-50995 lineage showed the highest levels of total monomeric anthocyanins, total isoflavones and carotenoids. In all three soybean linages monomeric anthocyanins were identified: cyanidin-3-O-glucoside, majority profile in all samples (262.27 to 720.45 mg / 100g), delphinidin-3-O-glucoside (184.41 to 316.72 mg / 100g) and petunidin-3-O-glucoside (6.34 to 153.06 mg / 100g). The isoflavones daidzein, glycitin, genistein were also identified at higher levels, and their aglycone forms, daidzein, glycitein and genistein. It was not found statistically significant difference between the total isoflavone content in BRM09-50995 linage (138.65 mg / 100 g) and BRM11-51400 (140.75 mg / 100 g), which had the highest content of this compound. The greater the DPPH antioxidant activity was observed in BRM09-50995 (1072.92 g / g DPPH), while BRM11-51400 showed the most significant results when used in the method of ABTS radical. Based on the results obtained in this study, it was found that the four soybean lineages are suitable for human consumption. |
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Godoy, Ronoel Luiz de Oliveira507.802.047-00http://lattes.cnpq.br/3671854931659782Felberg, IlanaGaldeano, Melicia CintiaCarvalho, Carlos Wanderlei Piler de121.994.117-45http://lattes.cnpq.br/0282669959921778Pereira, Joana de Novais2020-11-24T17:24:02Z2015-02-02PEREIRA, Joana de Novais. Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos. 2015. 73 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2015.https://tede.ufrrj.br/jspui/handle/jspui/4191The soybean with black coat (Glycine max (L.) Merrill) is attracting great interest to consumers and researchers because its consumption is associated with benefits to human health, in part associated with the presence of secondary metabolites, mainly isoflavones and anthocyanins. Embrapa has different strains of black soybeans that need to be assessed not only in relation to their agronomic characteristics, but also suitable for human consumption. Therefore, this study aimed to evaluate and compare four different strains of black soybean developed by Embrapa as the chemical composition, antinutritional factors, content and profile of isoflavones, anthocyanins, amino acids, carotenoids and antioxidant activity measured by DPPH method and ABTS. In respect to composition, there was no statistical difference (p> 0.05) between the protein (41.15 to 41.69 g/100) and moisture (from 10.41 to 10.61 g/100 g) in four soybean lines, while the ether extract values (19.12 to 19.79 g/100 g), carbohydrates (33.38 to 34.22 g / 100g) and ash (5.13 to 5.81 g / 100g ) varied statistically. The BRM11-51400 was the lineage with the most significant result for mineral as it showed the highest potassium content (2372.89 mg / 100g), most abundant mineral in all samples, phosphorus (753.56 mg / 100g ), magnesium (255.32 mg / 100 g) and calcium (257.82 mg / 100). It was also determined the concentration of manganese, zinc, copper, cobalt, selenium, iron and aluminum in significant amounts (9.04 to 11.52 mg / 100g). The higher sucrose content (6.46 g / 100 g) and raffinose (1.56g / 100g) was observed in BRM90-50901 while BRM11-51400 was the strain with the highest concentration of stachyose (5.24g /100g). Phytate varied from 11.91 to 14.88 mg / 100g, respectively. In relation to bioactive compounds, the BRM09-50995 lineage showed the highest levels of total monomeric anthocyanins, total isoflavones and carotenoids. In all three soybean linages monomeric anthocyanins were identified: cyanidin-3-O-glucoside, majority profile in all samples (262.27 to 720.45 mg / 100g), delphinidin-3-O-glucoside (184.41 to 316.72 mg / 100g) and petunidin-3-O-glucoside (6.34 to 153.06 mg / 100g). The isoflavones daidzein, glycitin, genistein were also identified at higher levels, and their aglycone forms, daidzein, glycitein and genistein. It was not found statistically significant difference between the total isoflavone content in BRM09-50995 linage (138.65 mg / 100 g) and BRM11-51400 (140.75 mg / 100 g), which had the highest content of this compound. The greater the DPPH antioxidant activity was observed in BRM09-50995 (1072.92 g / g DPPH), while BRM11-51400 showed the most significant results when used in the method of ABTS radical. Based on the results obtained in this study, it was found that the four soybean lineages are suitable for human consumption.O gr?o de soja com tegumento preto (Glycine max (L.) Merrill) vem despertando interesse de consumidores e pesquisadores devido o seu consumo estar associado com benef?cios ? sa?de humana, relacionados ? presen?a de metab?litos secund?rios, isoflavonas e principalmente das antocianinas. A Embrapa disp?e de diferentes linhagens de soja preta que precisam ser avaliadas n?o somente em rela??o ?s suas caracter?sticas agron?micas, mas se s?o adequadas ao consumo humano. O presente trabalho teve como objetivo geral avaliar e comparar quatro diferentes linhagens de soja preta desenvolvidas pela Embrapa quanto ? composi??o centesimal, fatores antinutricionais, teores de a??cares, perfil e teores de isoflavonas, antocianinas, amino?cidos, carotenoides, al?m da atividade antioxidante medida pelo m?todo DPPH e ABTS. Em rela??o ? composi??o centesimal, n?o foi observada diferen?a estat?stica entre os teores de prote?na (41,15 a 41,69 g/100) e umidade (10,41 a 10,61 g/100g) nas quatro linhagens de soja, enquanto os valores de extrato et?reo (19,12 a 19,79 g/100g), cinzas (5,13 a 5,81 g/100g) e carboidratos (33,38 a 34,22 g/100g) variaram estatisticamente (p?0,05). A BRM11-51400 foi a linhagem com o resultado mais expressivo para minerais, uma vez que apresentou o maior teor de pot?ssio (2372,89 mg/100g), mineral mais abundante em todas as amostras avaliadas, f?sforo (753,56 mg/100g), magn?sio (255,32 mg/100g) e c?lcio (257,82 mg/100). Foi tamb?m determinada a concentra??o de mangan?s, zinco, cobre, cobalto, sel?nio, alum?nio e ferro em teores significativos (9,04 a 11,52 mg/100g). O maior teor de sacarose (6,46 g/100g) e de rafinose (1,56 g/100g) foi observado na BRM09-50901, enquanto a BRM11-51400 foi a linhagem com a maior concentra??o de estaquiose (5,24 g/100g). O fitato ? um fator antinutricional presente em gr?o de soja, e foi detectado em teores que variaram de 11,91 a 14,88 mg/100g. Em rela??o aos compostos bioativos, a linhagem BRM09-50995, apresentou os teores mais elevados de antocianinas monom?ricas totais, isoflavonas totais e carotenoides totais. Foram identificadas nas linhagens de soja tr?s antocianinas monom?ricas: a cianidina-3-O-glicos?deo, perfil majorit?rio em todas as amostras (262,27 a 720,45 mg/100g), a delfinidina-3-O-glicos?deo (184,41 a 316,72 mg/100g) e a petunidina-3-O-glicos?deo (6,34 a 153,06 mg/100g). Foram tamb?m identificadas as isoflavonas: daidzina, glicitina e a genistina em maiores concentra??es, e suas respectivas formas agliconas, daidze?na, glicite?na e geniste?na. N?o foi observada diferen?a estat?stica entre os teores de isoflavonas totais nas linhagens BRM09-50995 (138,65 mg/100g) e BRM11-51400 (140,75 mg/100g), que apresentaram o maior teor deste composto. A maior capacidade antioxidante pelo m?todo DPPH foi observada na linhagem BRM09-50995 (1072,92 g/g DPPH), enquanto a BRM11-51400 apresentou o resultado mais expressivo quando utilizado o m?todo do radical ABTS. Embora a linhagem BRM09-50995 tenha apresentando os maiores teores de compostos bioativos, com base nos resultados obtidos nesse estudo, verificou-se que as quatro linhagens de soja s?o adequadas ao consumo humano.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-11-24T17:24:02Z No. of bitstreams: 1 2015 - Joana de Novais Pereira.pdf: 2767080 bytes, checksum: 04a4881c3f47e48b043ba5d458e72c67 (MD5)Made available in DSpace on 2020-11-24T17:24:02Z (GMT). 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dc.title.por.fl_str_mv |
Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos |
dc.title.alternative.eng.fl_str_mv |
Evaluation of different lines of black soybeans as theirs physical and chemical characteristics, nutritional and bioactive compounds |
title |
Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos |
spellingShingle |
Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos Pereira, Joana de Novais Glycine max composi??o centesimal compostos fen?licos atividade antioxidante Glycine max chemical composition phenolic compounds antioxidant activity Ci?ncia e Tecnologia de Alimentos |
title_short |
Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos |
title_full |
Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos |
title_fullStr |
Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos |
title_full_unstemmed |
Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos |
title_sort |
Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos |
author |
Pereira, Joana de Novais |
author_facet |
Pereira, Joana de Novais |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Godoy, Ronoel Luiz de Oliveira |
dc.contributor.advisor1ID.fl_str_mv |
507.802.047-00 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3671854931659782 |
dc.contributor.advisor-co1.fl_str_mv |
Felberg, Ilana |
dc.contributor.referee1.fl_str_mv |
Galdeano, Melicia Cintia |
dc.contributor.referee2.fl_str_mv |
Carvalho, Carlos Wanderlei Piler de |
dc.contributor.authorID.fl_str_mv |
121.994.117-45 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0282669959921778 |
dc.contributor.author.fl_str_mv |
Pereira, Joana de Novais |
contributor_str_mv |
Godoy, Ronoel Luiz de Oliveira Felberg, Ilana Galdeano, Melicia Cintia Carvalho, Carlos Wanderlei Piler de |
dc.subject.por.fl_str_mv |
Glycine max composi??o centesimal compostos fen?licos atividade antioxidante |
topic |
Glycine max composi??o centesimal compostos fen?licos atividade antioxidante Glycine max chemical composition phenolic compounds antioxidant activity Ci?ncia e Tecnologia de Alimentos |
dc.subject.eng.fl_str_mv |
Glycine max chemical composition phenolic compounds antioxidant activity |
dc.subject.cnpq.fl_str_mv |
Ci?ncia e Tecnologia de Alimentos |
description |
The soybean with black coat (Glycine max (L.) Merrill) is attracting great interest to consumers and researchers because its consumption is associated with benefits to human health, in part associated with the presence of secondary metabolites, mainly isoflavones and anthocyanins. Embrapa has different strains of black soybeans that need to be assessed not only in relation to their agronomic characteristics, but also suitable for human consumption. Therefore, this study aimed to evaluate and compare four different strains of black soybean developed by Embrapa as the chemical composition, antinutritional factors, content and profile of isoflavones, anthocyanins, amino acids, carotenoids and antioxidant activity measured by DPPH method and ABTS. In respect to composition, there was no statistical difference (p> 0.05) between the protein (41.15 to 41.69 g/100) and moisture (from 10.41 to 10.61 g/100 g) in four soybean lines, while the ether extract values (19.12 to 19.79 g/100 g), carbohydrates (33.38 to 34.22 g / 100g) and ash (5.13 to 5.81 g / 100g ) varied statistically. The BRM11-51400 was the lineage with the most significant result for mineral as it showed the highest potassium content (2372.89 mg / 100g), most abundant mineral in all samples, phosphorus (753.56 mg / 100g ), magnesium (255.32 mg / 100 g) and calcium (257.82 mg / 100). It was also determined the concentration of manganese, zinc, copper, cobalt, selenium, iron and aluminum in significant amounts (9.04 to 11.52 mg / 100g). The higher sucrose content (6.46 g / 100 g) and raffinose (1.56g / 100g) was observed in BRM90-50901 while BRM11-51400 was the strain with the highest concentration of stachyose (5.24g /100g). Phytate varied from 11.91 to 14.88 mg / 100g, respectively. In relation to bioactive compounds, the BRM09-50995 lineage showed the highest levels of total monomeric anthocyanins, total isoflavones and carotenoids. In all three soybean linages monomeric anthocyanins were identified: cyanidin-3-O-glucoside, majority profile in all samples (262.27 to 720.45 mg / 100g), delphinidin-3-O-glucoside (184.41 to 316.72 mg / 100g) and petunidin-3-O-glucoside (6.34 to 153.06 mg / 100g). The isoflavones daidzein, glycitin, genistein were also identified at higher levels, and their aglycone forms, daidzein, glycitein and genistein. It was not found statistically significant difference between the total isoflavone content in BRM09-50995 linage (138.65 mg / 100 g) and BRM11-51400 (140.75 mg / 100 g), which had the highest content of this compound. The greater the DPPH antioxidant activity was observed in BRM09-50995 (1072.92 g / g DPPH), while BRM11-51400 showed the most significant results when used in the method of ABTS radical. Based on the results obtained in this study, it was found that the four soybean lineages are suitable for human consumption. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-02-02 |
dc.date.accessioned.fl_str_mv |
2020-11-24T17:24:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PEREIRA, Joana de Novais. Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos. 2015. 73 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2015. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/jspui/4191 |
identifier_str_mv |
PEREIRA, Joana de Novais. Avalia??o de diferentes linhagens de soja preta quanto as suas caracter?sticas f?sico-qu?mica, nutricionais e compostos bioativos. 2015. 73 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2015. |
url |
https://tede.ufrrj.br/jspui/handle/jspui/4191 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.references.por.fl_str_mv |
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