Isolamento de constituintes químicos, estudo biológico e caracterização de extratos de Croton grewioides Baill. através de fingerprint cromatográfico e PCA

Detalhes bibliográficos
Ano de defesa: 2019
Autor(a) principal: Prado, Vilma Menezes de Jesus
Orientador(a): Moraes, Valéria Regina de Souza
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Não Informado pela instituição
Programa de Pós-Graduação: Pós-Graduação em Química
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Plantas medicinais
Plantas da caatinga
Euphorbiaceae
Cromatogramas
Agentes antineoplásticos
Croton grewioides Baill.
Cromatogramas fingerprint
Flavonoides
Atividade antitumoral
Atividade anticolinesterásica
Inibidores de colinesterase
Palavras-chave em Inglês:
Fingerprint chromatograms
Flavonoids
Antitumoral activity
Anticholinesterasic activity
Área do conhecimento CNPq:
CIENCIAS EXATAS E DA TERRA::QUIMICA
Link de acesso: http://ri.ufs.br/jspui/handle/riufs/10994
Resumo: The species Croton grewioides Baill., popularly known as "canelinha" or "canelinha-de-odor", has been used for the treatment of influenza, cough, fever and headache, however the study of its phytochemical composition is limited. A method by liquid chromatography for the differentiation of leaf extracts (hydroalcoholic, aqueous and methanolic) of four accessions of C. grewioides from two collections (winter and summer) has been developed. Some chemical constituents from aqueous extract of accession 107 (summer collection) were isolated by semi-preparative LC, in addition to the evaluation of in vitro activities against the tumor cell lines HepG2 (human hepatocellular carcinoma) and HL-60 (human leukemia) and anticholinesterase of the extracts and the partitions from methanolic extract.Thus, the fingerprint chromatograms of all extracts were obtained for the first time, which could be compared with the aid of chemometric tools. The results showed that only the chemical profile of the methanolic extracts of accessions 101 and 113 were influenced by the seasonality. The structures of three isolated metabolites were identified by 1H and 13C NMR (uni and bidimensional), namely: quercetin-3-O- [α-Rhamnopyranosyl- (1-2) -α- Rhamnopyranosyl- (1-6) -β-glucopyranoside], quercetin-3-O-glucoside and quercetin-3-O-methyl. Four hexanic partitions were considered active against the HL-60 cell, exhibiting inhibition values of 83.75; 84.43; 84.66 and 92.93%.
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spelling Prado, Vilma Menezes de JesusMoraes, Valéria Regina de Souza2019-04-17T17:14:54Z2019-04-17T17:14:54Z2019-02-28PRADO, Vilma Menezes de Jesus. Isolamento de constituintes químicos, estudo biológico e caracterização de extratos de Croton grewioides Baill. através de fingerprint cromatográfico e PCA. 2019. 159 f. Dissertação (Mestrado em Química) - Universidade Federal de Sergipe, São Cristóvão, SE, 2019.http://ri.ufs.br/jspui/handle/riufs/10994The species Croton grewioides Baill., popularly known as "canelinha" or "canelinha-de-odor", has been used for the treatment of influenza, cough, fever and headache, however the study of its phytochemical composition is limited. A method by liquid chromatography for the differentiation of leaf extracts (hydroalcoholic, aqueous and methanolic) of four accessions of C. grewioides from two collections (winter and summer) has been developed. Some chemical constituents from aqueous extract of accession 107 (summer collection) were isolated by semi-preparative LC, in addition to the evaluation of in vitro activities against the tumor cell lines HepG2 (human hepatocellular carcinoma) and HL-60 (human leukemia) and anticholinesterase of the extracts and the partitions from methanolic extract.Thus, the fingerprint chromatograms of all extracts were obtained for the first time, which could be compared with the aid of chemometric tools. The results showed that only the chemical profile of the methanolic extracts of accessions 101 and 113 were influenced by the seasonality. The structures of three isolated metabolites were identified by 1H and 13C NMR (uni and bidimensional), namely: quercetin-3-O- [α-Rhamnopyranosyl- (1-2) -α- Rhamnopyranosyl- (1-6) -β-glucopyranoside], quercetin-3-O-glucoside and quercetin-3-O-methyl. Four hexanic partitions were considered active against the HL-60 cell, exhibiting inhibition values of 83.75; 84.43; 84.66 and 92.93%.A espécie Croton grewioides Baill., popularmente conhecida como “canelinha” ou “canelinha-de-cheiro”, tem sido utilizada para o tratamento da gripe, no combate à tosse, febre e cefaléia, porém o estudo de sua composição fitoquímica é limitado. Um método por cromatografia líquida para diferenciação dos extratos (hidroalcoólico, aquoso e metanólico) de folhas de quatro acessos de C. grewioides a partir de duas coletas (inverno e verão) foi desenvolvido. Alguns constituintes químicos do extrato aquoso do acesso 107 (coleta de verão) foram isolados por CLAE semipreparativa, além da avaliação das atividades antitumoral in vitro frente as linhagens de células HepG2 (carcinoma hepatocelular humano) e HL-60 (leucemia humana) e anticolinesterásica dos extratos e das partições do extrato metanólico. Assim, foram obtidos pela primeira vez os cromatogramas fingerprint de todos os extratos, os quais puderam ser comparados com o auxílio de ferramentas quimiométricas. Os resultados mostraram que apenas o perfil químico dos extratos metanólicos dos acessos 101 e 113 foram influenciados pela sazonalidade. As estruturas de três metabólitos isolados foram identificadas por RMN 1H e 13C (uni e bidimensionais), sendo eles a quercetina - 3 - O - [ α- ramnopiranosil- (1-2) - α- ramnopiranosil- (1-6) – β -glicopiranosídeo], quercetina-3-O-glicosídeo e quercetina-3-O-metil. Quatro partições hexânicas foram consideradas ativas frente a célula HL-60, exibindo valores de 83,75; 84,43; 84,66 e 92,93% de inibição.São Cristóvão, SEporPlantas medicinaisPlantas da caatingaEuphorbiaceaeCromatogramasAgentes antineoplásticosCroton grewioides Baill.Cromatogramas fingerprintFlavonoidesAtividade antitumoralAtividade anticolinesterásicaInibidores de colinesteraseFingerprint chromatogramsFlavonoidsAntitumoral activityAnticholinesterasic activityCIENCIAS EXATAS E DA TERRA::QUIMICAIsolamento de constituintes químicos, estudo biológico e caracterização de extratos de Croton grewioides Baill. através de fingerprint cromatográfico e PCAIsolation of chemical constituents, biological study and characterization of extracts from Croton grewioides Baill. through chromatographic fingerprint and PCAinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPós-Graduação em QuímicaUFSreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTEXTVILMA_MENEZES_JESUS_PRADO.pdf.txtVILMA_MENEZES_JESUS_PRADO.pdf.txtExtracted texttext/plain236456https://ri.ufs.br/jspui/bitstream/riufs/10994/3/VILMA_MENEZES_JESUS_PRADO.pdf.txt2767a3a1df4429f386a73f809f8bef0aMD53THUMBNAILVILMA_MENEZES_JESUS_PRADO.pdf.jpgVILMA_MENEZES_JESUS_PRADO.pdf.jpgGenerated Thumbnailimage/jpeg1499https://ri.ufs.br/jspui/bitstream/riufs/10994/4/VILMA_MENEZES_JESUS_PRADO.pdf.jpg269ad396ac53247bce6baf73c93c4e68MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/10994/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALVILMA_MENEZES_JESUS_PRADO.pdfVILMA_MENEZES_JESUS_PRADO.pdfapplication/pdf7087962https://ri.ufs.br/jspui/bitstream/riufs/10994/2/VILMA_MENEZES_JESUS_PRADO.pdf6162fb8281a40db6bf12674091a49f35MD52riufs/109942019-04-17 14:14:55.289oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2019-04-17T17:14:55Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.pt_BR.fl_str_mv Isolamento de constituintes químicos, estudo biológico e caracterização de extratos de Croton grewioides Baill. através de fingerprint cromatográfico e PCA
dc.title.alternative.por.fl_str_mv Isolation of chemical constituents, biological study and characterization of extracts from Croton grewioides Baill. through chromatographic fingerprint and PCA
title Isolamento de constituintes químicos, estudo biológico e caracterização de extratos de Croton grewioides Baill. através de fingerprint cromatográfico e PCA
spellingShingle Isolamento de constituintes químicos, estudo biológico e caracterização de extratos de Croton grewioides Baill. através de fingerprint cromatográfico e PCA
Prado, Vilma Menezes de Jesus
Plantas medicinais
Plantas da caatinga
Euphorbiaceae
Cromatogramas
Agentes antineoplásticos
Croton grewioides Baill.
Cromatogramas fingerprint
Flavonoides
Atividade antitumoral
Atividade anticolinesterásica
Inibidores de colinesterase
Fingerprint chromatograms
Flavonoids
Antitumoral activity
Anticholinesterasic activity
CIENCIAS EXATAS E DA TERRA::QUIMICA
title_short Isolamento de constituintes químicos, estudo biológico e caracterização de extratos de Croton grewioides Baill. através de fingerprint cromatográfico e PCA
title_full Isolamento de constituintes químicos, estudo biológico e caracterização de extratos de Croton grewioides Baill. através de fingerprint cromatográfico e PCA
title_fullStr Isolamento de constituintes químicos, estudo biológico e caracterização de extratos de Croton grewioides Baill. através de fingerprint cromatográfico e PCA
title_full_unstemmed Isolamento de constituintes químicos, estudo biológico e caracterização de extratos de Croton grewioides Baill. através de fingerprint cromatográfico e PCA
title_sort Isolamento de constituintes químicos, estudo biológico e caracterização de extratos de Croton grewioides Baill. através de fingerprint cromatográfico e PCA
author Prado, Vilma Menezes de Jesus
author_facet Prado, Vilma Menezes de Jesus
author_role author
dc.contributor.author.fl_str_mv Prado, Vilma Menezes de Jesus
dc.contributor.advisor1.fl_str_mv Moraes, Valéria Regina de Souza
contributor_str_mv Moraes, Valéria Regina de Souza
dc.subject.por.fl_str_mv Plantas medicinais
Plantas da caatinga
Euphorbiaceae
Cromatogramas
Agentes antineoplásticos
Croton grewioides Baill.
Cromatogramas fingerprint
Flavonoides
Atividade antitumoral
Atividade anticolinesterásica
Inibidores de colinesterase
topic Plantas medicinais
Plantas da caatinga
Euphorbiaceae
Cromatogramas
Agentes antineoplásticos
Croton grewioides Baill.
Cromatogramas fingerprint
Flavonoides
Atividade antitumoral
Atividade anticolinesterásica
Inibidores de colinesterase
Fingerprint chromatograms
Flavonoids
Antitumoral activity
Anticholinesterasic activity
CIENCIAS EXATAS E DA TERRA::QUIMICA
dc.subject.eng.fl_str_mv Fingerprint chromatograms
Flavonoids
Antitumoral activity
Anticholinesterasic activity
dc.subject.cnpq.fl_str_mv CIENCIAS EXATAS E DA TERRA::QUIMICA
description The species Croton grewioides Baill., popularly known as "canelinha" or "canelinha-de-odor", has been used for the treatment of influenza, cough, fever and headache, however the study of its phytochemical composition is limited. A method by liquid chromatography for the differentiation of leaf extracts (hydroalcoholic, aqueous and methanolic) of four accessions of C. grewioides from two collections (winter and summer) has been developed. Some chemical constituents from aqueous extract of accession 107 (summer collection) were isolated by semi-preparative LC, in addition to the evaluation of in vitro activities against the tumor cell lines HepG2 (human hepatocellular carcinoma) and HL-60 (human leukemia) and anticholinesterase of the extracts and the partitions from methanolic extract.Thus, the fingerprint chromatograms of all extracts were obtained for the first time, which could be compared with the aid of chemometric tools. The results showed that only the chemical profile of the methanolic extracts of accessions 101 and 113 were influenced by the seasonality. The structures of three isolated metabolites were identified by 1H and 13C NMR (uni and bidimensional), namely: quercetin-3-O- [α-Rhamnopyranosyl- (1-2) -α- Rhamnopyranosyl- (1-6) -β-glucopyranoside], quercetin-3-O-glucoside and quercetin-3-O-methyl. Four hexanic partitions were considered active against the HL-60 cell, exhibiting inhibition values of 83.75; 84.43; 84.66 and 92.93%.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-04-17T17:14:54Z
dc.date.available.fl_str_mv 2019-04-17T17:14:54Z
dc.date.issued.fl_str_mv 2019-02-28
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv PRADO, Vilma Menezes de Jesus. Isolamento de constituintes químicos, estudo biológico e caracterização de extratos de Croton grewioides Baill. através de fingerprint cromatográfico e PCA. 2019. 159 f. Dissertação (Mestrado em Química) - Universidade Federal de Sergipe, São Cristóvão, SE, 2019.
dc.identifier.uri.fl_str_mv http://ri.ufs.br/jspui/handle/riufs/10994
identifier_str_mv PRADO, Vilma Menezes de Jesus. Isolamento de constituintes químicos, estudo biológico e caracterização de extratos de Croton grewioides Baill. através de fingerprint cromatográfico e PCA. 2019. 159 f. Dissertação (Mestrado em Química) - Universidade Federal de Sergipe, São Cristóvão, SE, 2019.
url http://ri.ufs.br/jspui/handle/riufs/10994
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dc.publisher.program.fl_str_mv Pós-Graduação em Química
dc.publisher.initials.fl_str_mv UFS
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFS
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