Avaliação de adsorvente alternativo na extração em fase sólida de resíduos de agrotóxicos em tecido muscular de tilápia (Oreochromis niloticus)

• Ano de defesa: 2017
• Autor(a) principal: Santos, Luiza Maria Ramos dos
• Orientador(a): Navickiene, Sandro
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Sergipe
• Programa de Pós-Graduação: Pós-Graduação em Química
• País: Brasil
• Palavras-chave em Português: Química; Tilápia (peixe); Criação de peixes; Produtos químicos agrícolas; Oreochromis niloticus; Agrotóxicos; DMFS; Piscicultura
• Palavras-chave em Inglês: GCMS/MS; Tilapia; Pesticides; MSPD; Pisciculture
• Área do conhecimento CNPq: CIENCIAS EXATAS E DA TERRA::QUIMICA
• Link de acesso: https://ri.ufs.br/handle/riufs/6075

The fish products is the most commonly used animal protein in the world. Brazil stands out for pisciculture with emphasis on the production of tilapia (Oreochromis niloticus). Fish farming is subject to attack by pests and pesticide application has been used as a form of control. This work aims at the development of an effective methodology based on the matrix solid phase dispersion (MSPD) and gas chromatography/spectrometry tandem mass (GCMS/ MS) techniques for determination of residues of aldrin, ametrina, bromopropylate, bromuconazole, cypermethrin, chlorpyrifos, chlorpropham, coumaphos, esfenvalerate, etion, permethrin, pirimicarb, pirimiphos methyl, procymidone, vinclozolin pesticides in muscle tissue of tilapia. Therefore, optimization of chromatographic and evaluation of best extraction conditions proposed were performed. The best quantitative response conditions were obtained with 0.25 g of sample homogenized with 0.75 g of adsorbent, using 0.5 g of auxiliary adsorbent, eluted with 5 mL of acetonitrile. The biochar produced from coconut wastes was tested as an adsorbent for DMFS extraction too and presented satisfactory recovery results between 70 ± 1.38% and 120 ± 5.55% at concentration levels 0.02 to 0.1 μg g-1. The parameters evaluated for the validation of the method were: linearity and sensitivity, obtaining a good sensitivity and linear response with coefficients of 0.9999 in the range of 0.01 to 4 μg g-1; selectivity, through matrix effect studies; accuracy and precision with recovery values between 70 - 120% and coefficients ranging from 0 to 16.69% for the concentration levels 0.02 to 0.1 μg g-1 (n = 5) and detection limits and quantification in the range of 0.01 to 0.0009 μg g-1 respectively. The method developed was adequate for the determination of residues of pesticides in samples of tilapia.