Efeitos da associação da aflatoxina B1 com aspartame em parâmetros bioquímicos e comportamentais em ratos
Ano de defesa: | 2020 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Rurais |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos
|
Departamento: |
Ciência e Tecnologia dos Alimentos
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/22936 |
Resumo: | Mycotoxins are substances produced by fungi, natural contaminants of various foods. Aflatoxins are mycotoxins produced mainly by fungi of the Aspergillus genus, with aflatoxin B1 (AFB1) being the most frequent and the most toxic. AFB1 has carcinogenic, mutagenic and teratogenic effects, is converted in the liver to 8,9-epoxide, a metabolite that reacts with cellular macromolecules, including proteins, RNA and DNA. In this way, AFB1 alters hematological parameters and promotes an imbalance in the oxidative system, especially in antioxidant enzymes. Sweeteners are substances used as substitutes for sucrose, among them aspartame (ASP), which is widely used in food formulations and in the pharmaceutical industry. The toxicity mechanism of ASP is mainly based on the induction of oxidative stress and can act on different tissues. Considering the presence of both AFB1 and ASP in a meal, as well as their mechanisms of toxicity, they can promote the development of oxidative stress, imbalance in enzyme defenses and facilitate the development of behavioral changes. In this study, we investigated the exposure to AFB1 (250 μg / kg, ig) and / or ASP (75mg / kg, ig) in the animals' behavior through the Open Field, Marble Burying test, Nesting test and Splash test, and biochemical parameters after 7 and 14 days. The results showed changes in behavioral parameters, evidenced by the increase in time spent in the center in the open field test, increase in the number of balls buried and decrease in the time of cleaning the face in the sucrose spray test in the animals treated with AFB1 + ASP after 7 and 14 days. There was a change in oxidative stress markers, represented by a decrease in the activity of the enzyme catalase (CAT) in the liver and kidney after 7 days of exposure and an increase in activity after 14 days in all groups. Glutathione-S-transferase (GST) increased in the liver and kidney after 7 and 14 days, the ascorbic acid content decreased in the liver, cortex and hippocampus only in the longest treatment. Likewise, non-protein thiols (NPSH) and antioxidant power using reduced iron (FRAP) decreased in the liver, kidney, cortex and hippocampus only after 14 days of exposure. The lipid peroxidation determined by thiobarbituric acid reactive substances (TBARS) increased in the 7-day treatment only in the hippocampus, and after 14 days of exposure to AFB1 + ASP there was an increase in this marker in the liver, kidney, cortex and hippocampus. We observed a reduction in SOD-2 immunoreactivity in the hippocampus after treatment with AFB1 or ASP. It can be concluded that the administration of AFB1 and ASP alter oxidative parameters in the liver, kidney, cerebral cortex and hippocampus, both isolated and associated, in addition to promoting changes in the parameters related to depression and anxiety analyzed in the open field, Marble Burying test and Splash test . Thus, we emphasize the importance of knowing the toxic effects and the synergism that can occur between these food components, which may be able to promote an imbalance and thus affect and/or facilitate the development of diseases. |
id |
UFSM-20_009fcbfe40dd6e8a28431f15e2d4360a |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/22936 |
network_acronym_str |
UFSM-20 |
network_name_str |
Manancial - Repositório Digital da UFSM |
repository_id_str |
|
spelling |
2021-11-23T18:34:15Z2021-11-23T18:34:15Z2020-03-11http://repositorio.ufsm.br/handle/1/22936Mycotoxins are substances produced by fungi, natural contaminants of various foods. Aflatoxins are mycotoxins produced mainly by fungi of the Aspergillus genus, with aflatoxin B1 (AFB1) being the most frequent and the most toxic. AFB1 has carcinogenic, mutagenic and teratogenic effects, is converted in the liver to 8,9-epoxide, a metabolite that reacts with cellular macromolecules, including proteins, RNA and DNA. In this way, AFB1 alters hematological parameters and promotes an imbalance in the oxidative system, especially in antioxidant enzymes. Sweeteners are substances used as substitutes for sucrose, among them aspartame (ASP), which is widely used in food formulations and in the pharmaceutical industry. The toxicity mechanism of ASP is mainly based on the induction of oxidative stress and can act on different tissues. Considering the presence of both AFB1 and ASP in a meal, as well as their mechanisms of toxicity, they can promote the development of oxidative stress, imbalance in enzyme defenses and facilitate the development of behavioral changes. In this study, we investigated the exposure to AFB1 (250 μg / kg, ig) and / or ASP (75mg / kg, ig) in the animals' behavior through the Open Field, Marble Burying test, Nesting test and Splash test, and biochemical parameters after 7 and 14 days. The results showed changes in behavioral parameters, evidenced by the increase in time spent in the center in the open field test, increase in the number of balls buried and decrease in the time of cleaning the face in the sucrose spray test in the animals treated with AFB1 + ASP after 7 and 14 days. There was a change in oxidative stress markers, represented by a decrease in the activity of the enzyme catalase (CAT) in the liver and kidney after 7 days of exposure and an increase in activity after 14 days in all groups. Glutathione-S-transferase (GST) increased in the liver and kidney after 7 and 14 days, the ascorbic acid content decreased in the liver, cortex and hippocampus only in the longest treatment. Likewise, non-protein thiols (NPSH) and antioxidant power using reduced iron (FRAP) decreased in the liver, kidney, cortex and hippocampus only after 14 days of exposure. The lipid peroxidation determined by thiobarbituric acid reactive substances (TBARS) increased in the 7-day treatment only in the hippocampus, and after 14 days of exposure to AFB1 + ASP there was an increase in this marker in the liver, kidney, cortex and hippocampus. We observed a reduction in SOD-2 immunoreactivity in the hippocampus after treatment with AFB1 or ASP. It can be concluded that the administration of AFB1 and ASP alter oxidative parameters in the liver, kidney, cerebral cortex and hippocampus, both isolated and associated, in addition to promoting changes in the parameters related to depression and anxiety analyzed in the open field, Marble Burying test and Splash test . Thus, we emphasize the importance of knowing the toxic effects and the synergism that can occur between these food components, which may be able to promote an imbalance and thus affect and/or facilitate the development of diseases.Micotoxinas são substâncias produzidas por fungos, contaminantes naturais de diversos alimentos. As aflatoxinas são micotoxinas produzidas principalmente pelos fungos do gênero Aspergillus, sendo a aflatoxina B1 (AFB1) a mais frequente e a mais tóxica. A AFB1 apresenta efeitos carcinogênicos, mutagênicos e teratogênicos, é convertida no fígado em 8,9-epóxido, um metabólito que reage com macromoléculas celulares, incluindo proteínas, RNA e DNA. Desta forma, a AFB1 altera parâmetros hematológicos e promove um desequilíbrio no sistema oxidativo, especialmente nas enzimas antioxidantes. Os adoçantes são substâncias utilizadas como substitutos da sacarose, dentre eles destaca-se o aspartame (ASP), o qual é amplamente utilizado em formulações alimentares e na indústria farmacêutica. O mecanismo de toxicidade do ASP baseia-se principalmente na indução de estresse oxidativo e pode atuar em diferentes tecidos. Considerando a presença tanto de AFB1 como de ASP numa refeição, bem como seus mecanismos de toxicidade, eles podem promover o desenvolvimento de quadros de estresse oxidativo, desequilíbrio nas defesas enzimáticas e facilitar o desenvolvimento de alterações comportamentais. Neste estudo, investigamos a exposição a AFB1 (250 μg/kg, i.g.) e/ou ASP (75mg/kg, i.g.) no comportamento dos animais através dos testes de Campo Aberto, Bolinhas enterradas, Construção de Ninho e Borrifagem de Sacarose, bem como em parâmetros bioquímicos após 7 e 14 dias. Os resultados mostraram alterações nos parâmetros comportamentais, evidenciados pelo aumento no tempo gasto no centro no teste de campo aberto, aumento no número de bolinhas enterradas e diminuição no tempo de limpeza da face no teste de borrifagem de sacarose nos animais tratados com AFB1+ASP após 7 e 14 dias. Houve alteração nos marcadores de estrese oxidativo, representados pela diminuição da atividade da enzima catalase (CAT) no fígado e rim após 7 dias de exposição e aumento na atividade após 14 dias em todos os grupos. A glutationa-S-transferase (GST) aumentou no fígado e rim após 7 e 14 dias, o conteúdo de ácido ascórbico diminuiu no fígado, córtex e hipocampo somente no tratamento mais longo. Da mesma forma, os tióis não-proteicos (NPSH) e poder antioxidante usando ferro reduzido (FRAP) diminuíram no fígado, rim, córtex e hipocampo somente após 14 dias de exposição. A peroxidação lipídica determinada através das substâncias reativas ao ácido tiobarbitúrico (TBARS) aumentou no tratamento de 7 dias somente no hipocampo, e após 14 dias de exposição à AFB1+ASP ocorreu aumento deste marcador no fígado, rim, córtex e hipocampo. Observamos uma redução na imunorreatividade da SOD-2 no hipocampo após o tratamento com AFB1 ou ASP. Pode-se concluir que a administração de AFB1 e ASP alteram parâmetros oxidativos no fígado, rim, córtex cerebral e hipocampo, tanto isolados como associados, além de promover mudanças nos parâmetros relacionados à depressão e ansiedade analisados nos estes de campo aberto, bolinhas e borrifagem de sacarose. Desta forma, salientamos a importância do conhecimento dos efeitos tóxicos e do sinergismo que pode ocorrer entre estes componentes alimentares, os quais podem ser capazes de promover um desequilíbrio e assim acometer e/ou facilitar o desenvolvimento de doenças.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESConselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqFundação de Amparo à Pesquisa do Estado do Rio Grande do Sul - FAPERGSporUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em Ciência e Tecnologia dos AlimentosUFSMBrasilCiência e Tecnologia dos AlimentosAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAdoçante artificialMicotoxinaEstresse oxidativoComportamentoArtificial sweetenerMycotoxinOxidative stressBehaviourCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOSEfeitos da associação da aflatoxina B1 com aspartame em parâmetros bioquímicos e comportamentais em ratosEffects of the association of aflatoxin B1 with aspartame in biochemical and behavioral parameters in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisFurian, Ana Fláviahttp://lattes.cnpq.br/0865191340133424Freitas, Catiuscia Molz deLima, Frederico DinizGuerra, Gustavo PetriBochi, Guilherme Vargashttp://lattes.cnpq.br/2027406169919954Souto, Naieli Schiefelbein500700000006600600600600600600600f74743c0-5489-4227-8a03-2bf9fa9bfeb2e4c44fe4-5c3e-4540-83a0-e1979eb40d16e7124024-298e-4136-9878-a5aa1be51a7d09597606-19d6-4a21-a6bd-cbbcd63b511818d4abbf-ab76-4e90-a7cd-8b0d2fe1e83d8942cbc4-805f-4793-b343-70e6ecb45178reponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMLICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/22936/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53ORIGINALTES_PPGCTA_2020_SOUTO_NAIELI.pdfTES_PPGCTA_2020_SOUTO_NAIELI.pdfTese de doutoradoapplication/pdf8748571http://repositorio.ufsm.br/bitstream/1/22936/1/TES_PPGCTA_2020_SOUTO_NAIELI.pdfae7514f61b018db928d0da4efe7dd654MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/22936/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52TEXTTES_PPGCTA_2020_SOUTO_NAIELI.pdf.txtTES_PPGCTA_2020_SOUTO_NAIELI.pdf.txtExtracted texttext/plain211969http://repositorio.ufsm.br/bitstream/1/22936/4/TES_PPGCTA_2020_SOUTO_NAIELI.pdf.txt97c2f9e64782d7337d7cd84c63ef8c17MD54THUMBNAILTES_PPGCTA_2020_SOUTO_NAIELI.pdf.jpgTES_PPGCTA_2020_SOUTO_NAIELI.pdf.jpgIM Thumbnailimage/jpeg4236http://repositorio.ufsm.br/bitstream/1/22936/5/TES_PPGCTA_2020_SOUTO_NAIELI.pdf.jpg7cce09ae3ce09942ef6ea948086d3ee2MD551/229362021-11-24 03:03:12.677oai:repositorio.ufsm.br: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ório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132021-11-24T06:03:12Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Efeitos da associação da aflatoxina B1 com aspartame em parâmetros bioquímicos e comportamentais em ratos |
dc.title.alternative.eng.fl_str_mv |
Effects of the association of aflatoxin B1 with aspartame in biochemical and behavioral parameters in rats |
title |
Efeitos da associação da aflatoxina B1 com aspartame em parâmetros bioquímicos e comportamentais em ratos |
spellingShingle |
Efeitos da associação da aflatoxina B1 com aspartame em parâmetros bioquímicos e comportamentais em ratos Souto, Naieli Schiefelbein Adoçante artificial Micotoxina Estresse oxidativo Comportamento Artificial sweetener Mycotoxin Oxidative stress Behaviour CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
title_short |
Efeitos da associação da aflatoxina B1 com aspartame em parâmetros bioquímicos e comportamentais em ratos |
title_full |
Efeitos da associação da aflatoxina B1 com aspartame em parâmetros bioquímicos e comportamentais em ratos |
title_fullStr |
Efeitos da associação da aflatoxina B1 com aspartame em parâmetros bioquímicos e comportamentais em ratos |
title_full_unstemmed |
Efeitos da associação da aflatoxina B1 com aspartame em parâmetros bioquímicos e comportamentais em ratos |
title_sort |
Efeitos da associação da aflatoxina B1 com aspartame em parâmetros bioquímicos e comportamentais em ratos |
author |
Souto, Naieli Schiefelbein |
author_facet |
Souto, Naieli Schiefelbein |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Furian, Ana Flávia |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0865191340133424 |
dc.contributor.referee1.fl_str_mv |
Freitas, Catiuscia Molz de |
dc.contributor.referee2.fl_str_mv |
Lima, Frederico Diniz |
dc.contributor.referee3.fl_str_mv |
Guerra, Gustavo Petri |
dc.contributor.referee4.fl_str_mv |
Bochi, Guilherme Vargas |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2027406169919954 |
dc.contributor.author.fl_str_mv |
Souto, Naieli Schiefelbein |
contributor_str_mv |
Furian, Ana Flávia Freitas, Catiuscia Molz de Lima, Frederico Diniz Guerra, Gustavo Petri Bochi, Guilherme Vargas |
dc.subject.por.fl_str_mv |
Adoçante artificial Micotoxina Estresse oxidativo Comportamento |
topic |
Adoçante artificial Micotoxina Estresse oxidativo Comportamento Artificial sweetener Mycotoxin Oxidative stress Behaviour CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
dc.subject.eng.fl_str_mv |
Artificial sweetener Mycotoxin Oxidative stress Behaviour |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
description |
Mycotoxins are substances produced by fungi, natural contaminants of various foods. Aflatoxins are mycotoxins produced mainly by fungi of the Aspergillus genus, with aflatoxin B1 (AFB1) being the most frequent and the most toxic. AFB1 has carcinogenic, mutagenic and teratogenic effects, is converted in the liver to 8,9-epoxide, a metabolite that reacts with cellular macromolecules, including proteins, RNA and DNA. In this way, AFB1 alters hematological parameters and promotes an imbalance in the oxidative system, especially in antioxidant enzymes. Sweeteners are substances used as substitutes for sucrose, among them aspartame (ASP), which is widely used in food formulations and in the pharmaceutical industry. The toxicity mechanism of ASP is mainly based on the induction of oxidative stress and can act on different tissues. Considering the presence of both AFB1 and ASP in a meal, as well as their mechanisms of toxicity, they can promote the development of oxidative stress, imbalance in enzyme defenses and facilitate the development of behavioral changes. In this study, we investigated the exposure to AFB1 (250 μg / kg, ig) and / or ASP (75mg / kg, ig) in the animals' behavior through the Open Field, Marble Burying test, Nesting test and Splash test, and biochemical parameters after 7 and 14 days. The results showed changes in behavioral parameters, evidenced by the increase in time spent in the center in the open field test, increase in the number of balls buried and decrease in the time of cleaning the face in the sucrose spray test in the animals treated with AFB1 + ASP after 7 and 14 days. There was a change in oxidative stress markers, represented by a decrease in the activity of the enzyme catalase (CAT) in the liver and kidney after 7 days of exposure and an increase in activity after 14 days in all groups. Glutathione-S-transferase (GST) increased in the liver and kidney after 7 and 14 days, the ascorbic acid content decreased in the liver, cortex and hippocampus only in the longest treatment. Likewise, non-protein thiols (NPSH) and antioxidant power using reduced iron (FRAP) decreased in the liver, kidney, cortex and hippocampus only after 14 days of exposure. The lipid peroxidation determined by thiobarbituric acid reactive substances (TBARS) increased in the 7-day treatment only in the hippocampus, and after 14 days of exposure to AFB1 + ASP there was an increase in this marker in the liver, kidney, cortex and hippocampus. We observed a reduction in SOD-2 immunoreactivity in the hippocampus after treatment with AFB1 or ASP. It can be concluded that the administration of AFB1 and ASP alter oxidative parameters in the liver, kidney, cerebral cortex and hippocampus, both isolated and associated, in addition to promoting changes in the parameters related to depression and anxiety analyzed in the open field, Marble Burying test and Splash test . Thus, we emphasize the importance of knowing the toxic effects and the synergism that can occur between these food components, which may be able to promote an imbalance and thus affect and/or facilitate the development of diseases. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-03-11 |
dc.date.accessioned.fl_str_mv |
2021-11-23T18:34:15Z |
dc.date.available.fl_str_mv |
2021-11-23T18:34:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/22936 |
url |
http://repositorio.ufsm.br/handle/1/22936 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
500700000006 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 600 600 |
dc.relation.authority.fl_str_mv |
f74743c0-5489-4227-8a03-2bf9fa9bfeb2 e4c44fe4-5c3e-4540-83a0-e1979eb40d16 e7124024-298e-4136-9878-a5aa1be51a7d 09597606-19d6-4a21-a6bd-cbbcd63b5118 18d4abbf-ab76-4e90-a7cd-8b0d2fe1e83d 8942cbc4-805f-4793-b343-70e6ecb45178 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Ciência e Tecnologia dos Alimentos |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/22936/3/license.txt http://repositorio.ufsm.br/bitstream/1/22936/1/TES_PPGCTA_2020_SOUTO_NAIELI.pdf http://repositorio.ufsm.br/bitstream/1/22936/2/license_rdf http://repositorio.ufsm.br/bitstream/1/22936/4/TES_PPGCTA_2020_SOUTO_NAIELI.pdf.txt http://repositorio.ufsm.br/bitstream/1/22936/5/TES_PPGCTA_2020_SOUTO_NAIELI.pdf.jpg |
bitstream.checksum.fl_str_mv |
2f0571ecee68693bd5cd3f17c1e075df ae7514f61b018db928d0da4efe7dd654 4460e5956bc1d1639be9ae6146a50347 97c2f9e64782d7337d7cd84c63ef8c17 7cce09ae3ce09942ef6ea948086d3ee2 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
|
_version_ |
1794524391203143680 |