Determinação de HPAs presentes na nutrição parenteral e avaliação da exposição de neonatos através da presença do 1-hidroxipireno na urina

• Ano de defesa: 2016
• Autor(a) principal: Barichello, Márcia Meister
• Orientador(a): Nascimento, Denise Bohrer do
• Banca de defesa: Thiesen, Flavia Valladão , Barros, Helena Maria Tannhauser , Noremberg, Simone Moraes da Silva , Cardoso, Carmem Dickow
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Santa Maria
• Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Farmacêuticas
• Departamento: Farmacologia
• País: BR
• Palavras-chave em Português: HPAs; Nutrição parenteral; 1-hidroxipireno; Recém-nascido; Cromatografia líquida de alta eficiência; Validação
• Palavras-chave em Inglês: PAHs; Parenteral nutrition; 1-hydroxypyrene; Newborn; HPLC; Validation
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
• Link de acesso: http://repositorio.ufsm.br/handle/1/3424

Carbon black, which has polycyclic aromatic hydrocarbons (PAHs) adsorbed on its surface, is used in the composition of rubber for pharmaceutical used in injectable drugs and parenteral formulations such as amino acid solutions and lipid emulsions. In this study, the contamination of parenteral preparations administered to newborn infants (NB) by PAHs was evaluated. Commercial solutions used in the composition of parenteral nutrition bags (NP) and the content of the bags administered to a group of ten RN during their period in the neonatal intensive care unit was analyzed. It was also analyzed the metabolite 1-hydroxypyrene in the urine of these infants, as well as in the urine of a group of infants who were not receiving NP (control group). Methods for determination of PAHs and 1-hydroxypyrene by high performance liquid chromatography using detection with DAD and fluorecence, respectively, were developed and validated for the samples of the study. PAHs were extracted from the samples using a polyethylene column. In the urine samples, extraction of metabolite was performed on a C-18 cartridge after its enzymatic hydrolysis. The results showed PAHs contamination in 56 out of 59 samples. Total PAHs concentration ranged from 0.013 mg/L to 10.562 mg/L. Pyrene was present in 50 out of 59 samples. The analysis revealed the presence of the metabolite 1- hydroxypyrene in the urine of babies who received NP, with concentration ranging from 0.039 mg/L to 0.544 mg/L (0.03 and 0.877 mmol/mol creatinine). In the urine of the control group the metabolite was not detected. Considering the toxicity of PAHs and the large volume usually administered in the NP, the results found in this study are worrying. The presence of PAHs in NP administered to newborns is even more important because they are the most vulnerable to adverse effects.