Análise fitoquímica e atividade farmacológica in vitro e in vivo de Schinus molle L.

Detalhes bibliográficos
Ano de defesa: 2019
Autor(a) principal: Lovatto, Marina Ortiz lattes
Orientador(a): Manfron, Melânia Palermo lattes
Banca de defesa: Machado, Michel Mansur lattes, Vaucher, Lauren Rosa Crossetti lattes
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Santa Maria
Centro de Ciências da Saúde
Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Farmacêuticas
Departamento: Farmacologia
País: Brasil
Palavras-chave em Português:
Planta medicinal
Caracterização química
Potencial terapêutico
Pleurisia
Palavras-chave em Inglês:
Medicinal plant
Chemical characterization
Therapeutic potential
Pleurisy
Área do conhecimento CNPq:
CNPQ::CIENCIAS DA SAUDE::FARMACIA
Link de acesso: http://repositorio.ufsm.br/handle/1/19571
Resumo: The species S. molle L. is widely distributed in Rio Grande do Sul. The aerial parts of S. molle are popularly used for different therapeutic purposes as antiinflammatory, anti-haemorrhagic, antiseptic, mild laxative, astringent, anti-asthmatic, digestive stimulant, menstrual, diuretic and tonic disorder. The need to seek new therapeutic alternatives for respiratory diseases, preferably with minimal side effects, justifies this work, both from the point of view of scientific and social interest. The present work aimed to obtain the crude extract of S. molle leaves by the maceration process, perform phytochemical analysis, identify and quantify compounds present in the extract, evaluate biological activities, such as anti-inflammatory and antimicrobial activity, as well as evaluate cyto and genotoxicity and antioxidant potential against reactive oxygen and nitrogen species. S. molle extract after analysis showed concentrations of 79,14; 231,67; 52,65 mg equivalents/g of extract that were determined by phytochemical dosages in S. molle extract for total phenols, tannins and flavonoids, respectively. S. molle was able to capture reactive oxygen and nitrogen species with antioxidant capacity with IC50 = 30.54 ± 0.41 μg/mL. It did not present cytotoxicity and genotoxicity, being a safety indicator. Antimicrobial activity was not significant in relation to the microorganisms Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Mycobaterium tuberculosis, M. abcessus, M. massiliense, M. fortuitum and M. smegmatis. The extract showed no hematological alterations, even in the group induced pleurisy with 2% carrageenan. The flavonoids rutin (58,63 mg/g) and quercetin (0,665 mg/g) were identified and quantified.
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spelling 2020-02-13T14:07:24Z2020-02-13T14:07:24Z2019-08-27http://repositorio.ufsm.br/handle/1/19571The species S. molle L. is widely distributed in Rio Grande do Sul. The aerial parts of S. molle are popularly used for different therapeutic purposes as antiinflammatory, anti-haemorrhagic, antiseptic, mild laxative, astringent, anti-asthmatic, digestive stimulant, menstrual, diuretic and tonic disorder. The need to seek new therapeutic alternatives for respiratory diseases, preferably with minimal side effects, justifies this work, both from the point of view of scientific and social interest. The present work aimed to obtain the crude extract of S. molle leaves by the maceration process, perform phytochemical analysis, identify and quantify compounds present in the extract, evaluate biological activities, such as anti-inflammatory and antimicrobial activity, as well as evaluate cyto and genotoxicity and antioxidant potential against reactive oxygen and nitrogen species. S. molle extract after analysis showed concentrations of 79,14; 231,67; 52,65 mg equivalents/g of extract that were determined by phytochemical dosages in S. molle extract for total phenols, tannins and flavonoids, respectively. S. molle was able to capture reactive oxygen and nitrogen species with antioxidant capacity with IC50 = 30.54 ± 0.41 μg/mL. It did not present cytotoxicity and genotoxicity, being a safety indicator. Antimicrobial activity was not significant in relation to the microorganisms Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Mycobaterium tuberculosis, M. abcessus, M. massiliense, M. fortuitum and M. smegmatis. The extract showed no hematological alterations, even in the group induced pleurisy with 2% carrageenan. The flavonoids rutin (58,63 mg/g) and quercetin (0,665 mg/g) were identified and quantified.A espécie S. molle L. é amplamente distribuída no Rio Grande do Sul. As partes aéreas da S. molle são utilizadas popularmente para diferentes finalidades terapêuticas como anti-inflamatória, anti-hemorrágica, antisséptico, laxativo leve, adstringente, antiasmática, estimulante digestivo, distúrbio menstrual, diurético e tônico. A necessidade de buscar novas alternativas terapêuticas para doenças respiratórias, de preferência, com o mínimo de efeitos colaterais, justifica este trabalho, tanto pelo ponto de vista do interesse cientifico, quanto pelo social. O presente trabalho teve por objetivo obter o extrato bruto das folhas de S. molle pelo processo de maceração, realizar análise fitoquímica, identificar e quantificar compostos presentes no extrato, avaliar atividades biológicas, como atividade antiinflamatória e antimicrobiana, bem como, avaliar cito e genotoxicidade e potencial antioxidante frente as espécies reativas de oxigênio e nitrogênio. O extrato de S. molle após as análises apresentou concentrações de 79,14; 231,67; 52,65 mg de equivalentes/g de extrato que foram determinadas por dosagens fitoquímicas no extrato de S. molle para fenóis totais, taninos e flavonoides, respectivamente. A S. molle mostrou-se capaz de capturar espécies reativas de oxigênio e nitrogênio com capacidade antioxidante com IC50=30,54±0,41 μg/mL. Não apresentou citotoxicidade e genotoxicidade, sendo um indicativo de segurança. A atividade antimicrobiana foi não significativa frente aos microrganismos Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa e Mycobaterium tuberculosis, M. abcessus, M. massiliense, M. fortuitum e M. smegmatis. O extrato não demostrou alterações hematológicas, mesmo no grupo induzido pleurisia com carragenina 2%. Os flavonoides rutina (58,63 mg/g) e quercetina (0,665 mg/g) foram identificados e quantificados.porUniversidade Federal de Santa MariaCentro de Ciências da SaúdePrograma de Pós-Graduação em Ciências FarmacêuticasUFSMBrasilFarmacologiaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessPlanta medicinalCaracterização químicaPotencial terapêuticoPleurisiaMedicinal plantChemical characterizationTherapeutic potentialPleurisyCNPQ::CIENCIAS DA SAUDE::FARMACIAAnálise fitoquímica e atividade farmacológica in vitro e in vivo de Schinus molle L.Phytochemical analysis and pharmacological activity in vitro and in vivo of Schinus molle L.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisManfron, Melânia Palermohttp://lattes.cnpq.br/6737713729653264Machado, Michel Mansurhttp://lattes.cnpq.br/7651341120825287Vaucher, Lauren Rosa Crossettihttp://lattes.cnpq.br/7468913314581754http://lattes.cnpq.br/4830593822574848Lovatto, Marina Ortiz4003000000056000e590c6d-726c-423c-8af6-55070bd29d4a28daaaf8-2c43-49f3-b1b1-30e5293904150fde5880-3df4-4149-b64f-3ad796638807e65be09c-73ac-40fa-89ac-b5d8766255c7reponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGCF_2019_LOVATTO_MARINA.pdfDIS_PPGCF_2019_LOVATTO_MARINA.pdfDissertação de Mestradoapplication/pdf1201167http://repositorio.ufsm.br/bitstream/1/19571/1/DIS_PPGCF_2019_LOVATTO_MARINA.pdfe9d57366090fa646adf6e2dfe3610b12MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv Análise fitoquímica e atividade farmacológica in vitro e in vivo de Schinus molle L.
dc.title.alternative.eng.fl_str_mv Phytochemical analysis and pharmacological activity in vitro and in vivo of Schinus molle L.
title Análise fitoquímica e atividade farmacológica in vitro e in vivo de Schinus molle L.
spellingShingle Análise fitoquímica e atividade farmacológica in vitro e in vivo de Schinus molle L.
Lovatto, Marina Ortiz
Planta medicinal
Caracterização química
Potencial terapêutico
Pleurisia
Medicinal plant
Chemical characterization
Therapeutic potential
Pleurisy
CNPQ::CIENCIAS DA SAUDE::FARMACIA
title_short Análise fitoquímica e atividade farmacológica in vitro e in vivo de Schinus molle L.
title_full Análise fitoquímica e atividade farmacológica in vitro e in vivo de Schinus molle L.
title_fullStr Análise fitoquímica e atividade farmacológica in vitro e in vivo de Schinus molle L.
title_full_unstemmed Análise fitoquímica e atividade farmacológica in vitro e in vivo de Schinus molle L.
title_sort Análise fitoquímica e atividade farmacológica in vitro e in vivo de Schinus molle L.
author Lovatto, Marina Ortiz
author_facet Lovatto, Marina Ortiz
author_role author
dc.contributor.advisor1.fl_str_mv Manfron, Melânia Palermo
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/6737713729653264
dc.contributor.referee1.fl_str_mv Machado, Michel Mansur
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/7651341120825287
dc.contributor.referee2.fl_str_mv Vaucher, Lauren Rosa Crossetti
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/7468913314581754
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/4830593822574848
dc.contributor.author.fl_str_mv Lovatto, Marina Ortiz
contributor_str_mv Manfron, Melânia Palermo
Machado, Michel Mansur
Vaucher, Lauren Rosa Crossetti
dc.subject.por.fl_str_mv Planta medicinal
Caracterização química
Potencial terapêutico
Pleurisia
topic Planta medicinal
Caracterização química
Potencial terapêutico
Pleurisia
Medicinal plant
Chemical characterization
Therapeutic potential
Pleurisy
CNPQ::CIENCIAS DA SAUDE::FARMACIA
dc.subject.eng.fl_str_mv Medicinal plant
Chemical characterization
Therapeutic potential
Pleurisy
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS DA SAUDE::FARMACIA
description The species S. molle L. is widely distributed in Rio Grande do Sul. The aerial parts of S. molle are popularly used for different therapeutic purposes as antiinflammatory, anti-haemorrhagic, antiseptic, mild laxative, astringent, anti-asthmatic, digestive stimulant, menstrual, diuretic and tonic disorder. The need to seek new therapeutic alternatives for respiratory diseases, preferably with minimal side effects, justifies this work, both from the point of view of scientific and social interest. The present work aimed to obtain the crude extract of S. molle leaves by the maceration process, perform phytochemical analysis, identify and quantify compounds present in the extract, evaluate biological activities, such as anti-inflammatory and antimicrobial activity, as well as evaluate cyto and genotoxicity and antioxidant potential against reactive oxygen and nitrogen species. S. molle extract after analysis showed concentrations of 79,14; 231,67; 52,65 mg equivalents/g of extract that were determined by phytochemical dosages in S. molle extract for total phenols, tannins and flavonoids, respectively. S. molle was able to capture reactive oxygen and nitrogen species with antioxidant capacity with IC50 = 30.54 ± 0.41 μg/mL. It did not present cytotoxicity and genotoxicity, being a safety indicator. Antimicrobial activity was not significant in relation to the microorganisms Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Mycobaterium tuberculosis, M. abcessus, M. massiliense, M. fortuitum and M. smegmatis. The extract showed no hematological alterations, even in the group induced pleurisy with 2% carrageenan. The flavonoids rutin (58,63 mg/g) and quercetin (0,665 mg/g) were identified and quantified.
publishDate 2019
dc.date.issued.fl_str_mv 2019-08-27
dc.date.accessioned.fl_str_mv 2020-02-13T14:07:24Z
dc.date.available.fl_str_mv 2020-02-13T14:07:24Z
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url http://repositorio.ufsm.br/handle/1/19571
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http://creativecommons.org/licenses/by-nc-nd/4.0/
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dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências da Saúde
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências Farmacêuticas
dc.publisher.initials.fl_str_mv UFSM
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Farmacologia
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências da Saúde
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
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