Determinação multirresíduo de agrotóxicos por GC-MS/MS em plantas medicinais empregadas na produção de fitoterápicos
Ano de defesa: | 2018 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Química
|
Departamento: |
Química
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/18412 |
Resumo: | The use of exotic and Brazilian plants as a resource for therapeutic purposes in the prevention and treatment of diseases, have been evaluated as to the possibility of extracting plant active compounds, as well as the creation of medicinal products for human use. These medicines are called phytomedicines. So, the cultivation of these plants for this purpose should be monitored for the use of pesticides. Currently, Brazilian and international legislation has been showing interest in this public health issue. According to RDC n° 26/2014, the phytomedicines appropriately registered by ANVISA must be submitted to analysis of pesticide residues. In this study, we propose the QuEChERS method (an acronym for Quick, Easy, Cheap, Effective, Rugged and Safe) for the extraction and subsequent multi-residue determination of pesticides in Maytenus ilicifolia (espinheira-santa), Ginkgo biloba L. (Ginkgo biloba) and Mikania glomerata Spreng. (guaco) and Matricaria chamomilla (chamomile). In this method, 1 g of sample was taken into a 50 mL polypropylene tube and 10 mL of ultrapure water was added, followed by vortexing for 1 min and then soaking for 15 min. After 10 mL of acetonitrile (ACN) was added, and the tube was stirred again for 1 min. 4 g of MgSO4, 1 g of NaCl, 1 g of C6H5N3O7.2H2O, 0.5 g of C6H6Na2O7.H2O, followed by vigorous stirring for 1 min and then centrifugation at 6,000 rpm for 8 min at 4 °C. Afterwards, 1 mL of the supernatant was transferred to a 2 mL polypropylene tube containing 150 mg CaCl2 and 50 mg PSA. After that, the centrifugation step was carried out at 10,000 rpm for 8 min at 4 °C. The extract was filtered with 0.2 μm nylon filter. After filtration, the extract was injected into the GC-MS/MS chromatographic system. In the validated QuEChERS method, it obtained recovery results in the range of 70 to 120% and RSD of ≤ 20%. The limit of detection and limit of quantification obtained were 1.5 to 6 μg kg-1 and 5 to 20 μg kg-1, respectively. The method was validated for Ginkgo biloba L. and was applied for the extension of scope in the other matrices. |
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2019-09-24T18:59:14Z2019-09-24T18:59:14Z2018-08-06http://repositorio.ufsm.br/handle/1/18412The use of exotic and Brazilian plants as a resource for therapeutic purposes in the prevention and treatment of diseases, have been evaluated as to the possibility of extracting plant active compounds, as well as the creation of medicinal products for human use. These medicines are called phytomedicines. So, the cultivation of these plants for this purpose should be monitored for the use of pesticides. Currently, Brazilian and international legislation has been showing interest in this public health issue. According to RDC n° 26/2014, the phytomedicines appropriately registered by ANVISA must be submitted to analysis of pesticide residues. In this study, we propose the QuEChERS method (an acronym for Quick, Easy, Cheap, Effective, Rugged and Safe) for the extraction and subsequent multi-residue determination of pesticides in Maytenus ilicifolia (espinheira-santa), Ginkgo biloba L. (Ginkgo biloba) and Mikania glomerata Spreng. (guaco) and Matricaria chamomilla (chamomile). In this method, 1 g of sample was taken into a 50 mL polypropylene tube and 10 mL of ultrapure water was added, followed by vortexing for 1 min and then soaking for 15 min. After 10 mL of acetonitrile (ACN) was added, and the tube was stirred again for 1 min. 4 g of MgSO4, 1 g of NaCl, 1 g of C6H5N3O7.2H2O, 0.5 g of C6H6Na2O7.H2O, followed by vigorous stirring for 1 min and then centrifugation at 6,000 rpm for 8 min at 4 °C. Afterwards, 1 mL of the supernatant was transferred to a 2 mL polypropylene tube containing 150 mg CaCl2 and 50 mg PSA. After that, the centrifugation step was carried out at 10,000 rpm for 8 min at 4 °C. The extract was filtered with 0.2 μm nylon filter. After filtration, the extract was injected into the GC-MS/MS chromatographic system. In the validated QuEChERS method, it obtained recovery results in the range of 70 to 120% and RSD of ≤ 20%. The limit of detection and limit of quantification obtained were 1.5 to 6 μg kg-1 and 5 to 20 μg kg-1, respectively. The method was validated for Ginkgo biloba L. and was applied for the extension of scope in the other matrices.A utilização de plantas exóticas e brasileiras como recurso para fins terapêuticos na prevenção e tratamento de doenças têm sido avaliadas quanto à possibilidade de extração de compostos ativos vegetais, bem como a criação de medicamentos para uso humano. Estes medicamentos são denominados fitoterápicos. Dessa forma, o cultivo destas plantas para este fim, deve ser monitorado quanto ao uso de agrotóxicos. Atualmente, legislações brasileiras e internacionais vêm demonstrando interesse a esta questão de saúde pública. Conforme a RDC n° 26/2014, os medicamentos fitoterápicos devidamente registrados na ANVISA, devem ser submetidos à análise de resíduos de agrotóxicos. Neste estudo, propõe-se o método QuEChERS (do acrônimo inglês, Quick, Easy, Cheap, Effective, Rugged and Safe) para extração e posterior determinação multirresíduo de agrotóxicos em Maytenus ilicifolia (espinheira-santa), Ginkgo biloba L. (ginkgo biloba), Mikania glomerata Spreng. (guaco) e Matricaria chamomilla (camomila). Neste método utilizou-se, 1 g de amostra em tubo de polipropileno de 50 mL e adicionou-se 10 mL de água ultrapura, seguido de agitação em vórtex por 1 min e posterior repouso por 15 min. Após, adicionou-se 10 mL de acetonitrila (ACN), e o tubo é agitado por 1 min. Adicionou-se 4 g de MgSO4, 1 g de NaCl, 1 g de C6H5Na3O7.2H2O, 0,5 g de C6H6Na2O7.1,5H2O seguido de agitação vigorosa durante 1 min e depois centrifugação a 6.000 rpm, por 8 min a 4°C. Após, transferiu-se 1 mL do sobrenadante, para um tubo de polipropileno de 2 mL, contendo 150 mg CaCl2 e 50 mg PSA. Após, realizou-se etapa de centrifugação a 10.000 rpm, por 8 min e 4°C. O extrato foi filtrado com filtro de nylon de 0,2 μm. Após a filtração, o extrato foi injetado no sistema cromatográfico GC-MS/MS. No método QuEChERS validado obteve resultados de recuperação na faixa de 70% a 120% e RSD de ≤ 20%. O limite de detecção e o limite de quantificação obtidos foram de 1,5 a 6 μg kg-1 e 5 a 20 μg kg-1. O método foi validado para Ginkgo biloba L. e foi aplicado para a extensão de escopo nas outras matrizes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em QuímicaUFSMBrasilQuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessGC-MS/MSFitoterápicosPlantas medicinaisAgrotóxicosPhytomedicineMedicinal plantsPesticidesCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICADeterminação multirresíduo de agrotóxicos por GC-MS/MS em plantas medicinais empregadas na produção de fitoterápicosDetermination multiresidue of pesticides by GC-MS/MS in medicinal plants employed in the production of phytomedicinesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPrestes, Osmar Damianhttp://lattes.cnpq.br/9632234955509028Müller, Aline Lima Hermeshttp://lattes.cnpq.br/6859286861380431Gonçalves, Fabio Ferreirahttp://lattes.cnpq.br/1090108357021843http://lattes.cnpq.br/3758533308592430Santos, Pimpernelli Jonco dos100600000000600f79cc0c9-c923-4244-9c1f-c80d74674410dc3bf253-eb23-4c64-afa3-27f23dd38e023a5b86e4-a4fc-4124-b40b-b860a9eed50b3bd4dd64-ee3b-4e08-9102-1bfffb1ba94areponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGQUIMICA_2018_SANTOS_PIMPERNELLI.pdfDIS_PPGQUIMICA_2018_SANTOS_PIMPERNELLI.pdfDissertação de Mestradoapplication/pdf2548338http://repositorio.ufsm.br/bitstream/1/18412/1/DIS_PPGQUIMICA_2018_SANTOS_PIMPERNELLI.pdf999f71c80eb37561ab1108358beb68deMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Determinação multirresíduo de agrotóxicos por GC-MS/MS em plantas medicinais empregadas na produção de fitoterápicos |
dc.title.alternative.eng.fl_str_mv |
Determination multiresidue of pesticides by GC-MS/MS in medicinal plants employed in the production of phytomedicines |
title |
Determinação multirresíduo de agrotóxicos por GC-MS/MS em plantas medicinais empregadas na produção de fitoterápicos |
spellingShingle |
Determinação multirresíduo de agrotóxicos por GC-MS/MS em plantas medicinais empregadas na produção de fitoterápicos Santos, Pimpernelli Jonco dos GC-MS/MS Fitoterápicos Plantas medicinais Agrotóxicos Phytomedicine Medicinal plants Pesticides CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Determinação multirresíduo de agrotóxicos por GC-MS/MS em plantas medicinais empregadas na produção de fitoterápicos |
title_full |
Determinação multirresíduo de agrotóxicos por GC-MS/MS em plantas medicinais empregadas na produção de fitoterápicos |
title_fullStr |
Determinação multirresíduo de agrotóxicos por GC-MS/MS em plantas medicinais empregadas na produção de fitoterápicos |
title_full_unstemmed |
Determinação multirresíduo de agrotóxicos por GC-MS/MS em plantas medicinais empregadas na produção de fitoterápicos |
title_sort |
Determinação multirresíduo de agrotóxicos por GC-MS/MS em plantas medicinais empregadas na produção de fitoterápicos |
author |
Santos, Pimpernelli Jonco dos |
author_facet |
Santos, Pimpernelli Jonco dos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Prestes, Osmar Damian |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9632234955509028 |
dc.contributor.referee1.fl_str_mv |
Müller, Aline Lima Hermes |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6859286861380431 |
dc.contributor.referee2.fl_str_mv |
Gonçalves, Fabio Ferreira |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1090108357021843 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3758533308592430 |
dc.contributor.author.fl_str_mv |
Santos, Pimpernelli Jonco dos |
contributor_str_mv |
Prestes, Osmar Damian Müller, Aline Lima Hermes Gonçalves, Fabio Ferreira |
dc.subject.por.fl_str_mv |
GC-MS/MS Fitoterápicos Plantas medicinais Agrotóxicos |
topic |
GC-MS/MS Fitoterápicos Plantas medicinais Agrotóxicos Phytomedicine Medicinal plants Pesticides CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Phytomedicine Medicinal plants Pesticides |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
The use of exotic and Brazilian plants as a resource for therapeutic purposes in the prevention and treatment of diseases, have been evaluated as to the possibility of extracting plant active compounds, as well as the creation of medicinal products for human use. These medicines are called phytomedicines. So, the cultivation of these plants for this purpose should be monitored for the use of pesticides. Currently, Brazilian and international legislation has been showing interest in this public health issue. According to RDC n° 26/2014, the phytomedicines appropriately registered by ANVISA must be submitted to analysis of pesticide residues. In this study, we propose the QuEChERS method (an acronym for Quick, Easy, Cheap, Effective, Rugged and Safe) for the extraction and subsequent multi-residue determination of pesticides in Maytenus ilicifolia (espinheira-santa), Ginkgo biloba L. (Ginkgo biloba) and Mikania glomerata Spreng. (guaco) and Matricaria chamomilla (chamomile). In this method, 1 g of sample was taken into a 50 mL polypropylene tube and 10 mL of ultrapure water was added, followed by vortexing for 1 min and then soaking for 15 min. After 10 mL of acetonitrile (ACN) was added, and the tube was stirred again for 1 min. 4 g of MgSO4, 1 g of NaCl, 1 g of C6H5N3O7.2H2O, 0.5 g of C6H6Na2O7.H2O, followed by vigorous stirring for 1 min and then centrifugation at 6,000 rpm for 8 min at 4 °C. Afterwards, 1 mL of the supernatant was transferred to a 2 mL polypropylene tube containing 150 mg CaCl2 and 50 mg PSA. After that, the centrifugation step was carried out at 10,000 rpm for 8 min at 4 °C. The extract was filtered with 0.2 μm nylon filter. After filtration, the extract was injected into the GC-MS/MS chromatographic system. In the validated QuEChERS method, it obtained recovery results in the range of 70 to 120% and RSD of ≤ 20%. The limit of detection and limit of quantification obtained were 1.5 to 6 μg kg-1 and 5 to 20 μg kg-1, respectively. The method was validated for Ginkgo biloba L. and was applied for the extension of scope in the other matrices. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-08-06 |
dc.date.accessioned.fl_str_mv |
2019-09-24T18:59:14Z |
dc.date.available.fl_str_mv |
2019-09-24T18:59:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/18412 |
url |
http://repositorio.ufsm.br/handle/1/18412 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
100600000000 |
dc.relation.confidence.fl_str_mv |
600 |
dc.relation.authority.fl_str_mv |
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dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Química |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Química |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
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Universidade Federal de Santa Maria (UFSM) |
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UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
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Manancial - Repositório Digital da UFSM |
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