Parâmetros bioquímicos em jundiás expostos ao zinco
Ano de defesa: | 2014 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
|
Departamento: |
Bioquímica
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/11238 |
Resumo: | The contamination of the aquatic environment occurs as a consequence of industrial, agricultural and anthropogenic activities. Aquatic ecosystems are often contaminated with metals such as copper and zinc, which are essential in low concentrations but toxic at high concentrations. Thus, the aim of this study was to establish the mean lethal concentration (LC50) within 96 hours of zinc for silver catfish (Rhamdia quelen) and to evaluate the possible effects on biochemical parameters of fish exposed to 0.0 (control), 1.0 and 5.0 mg/L zinc for 96h. The parameters analyzed were reactive substances to thiobarbituric acid (TBARS), protein carbonyl, acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), non-protein thiols (NPSH) and Na+/K+-ATPase. The LC50 value found was 8.07 mg/L. The results of sublethal exposure showed that TBARS levels decreased in liver and brain at every tested Zn concentration. In gills, there was an increase on TBARS levels at 1.0 mg/L and a decrease at 5.0 mg/L, while no changes in muscle were observed. Exposure to zinc decreased protein carbonyls in liver of silver catfish exposed to 5.0 mg/L and gills in every tested concentration. In brain there were no changes in protein carbonyl and in muscle there was an increase at both concentrations. In liver, the SOD activity increased at both zinc concentrations. CAT activity in liver did not change at any concentration. The GST activity decreased in liver and brain increased at both concentrations used. In gills, GST increased in fish exposed to 1.0 mg/L and in muscle at 5.0 mg/L. NPSH levels showed no changes in liver either in gills. In brain there was an increase on NPSH levels at 5.0 mg/L, while the muscle declined at both concentrations. The AChE activity in brain decreased at both zinc concentrations, whereas there was no change in muscle activity for this enzyme. The Na+/K+-ATPase activity was inhibited in gill and intestine in every used concentration. We may conclude that zinc altered biochemical parameters of silver catfish and also the inhibition of brain AChE and Na+/K+-ATPase activity in intestine and gills may be used as biomarkers of waters contaminated by zinc. |
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2015-11-042015-11-042014-06-05LEITEMPERGER, Jossiele Wesz. Biochemical parameters in silver catfish exposed to zinc. 2014. 46 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014.http://repositorio.ufsm.br/handle/1/11238The contamination of the aquatic environment occurs as a consequence of industrial, agricultural and anthropogenic activities. Aquatic ecosystems are often contaminated with metals such as copper and zinc, which are essential in low concentrations but toxic at high concentrations. Thus, the aim of this study was to establish the mean lethal concentration (LC50) within 96 hours of zinc for silver catfish (Rhamdia quelen) and to evaluate the possible effects on biochemical parameters of fish exposed to 0.0 (control), 1.0 and 5.0 mg/L zinc for 96h. The parameters analyzed were reactive substances to thiobarbituric acid (TBARS), protein carbonyl, acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), non-protein thiols (NPSH) and Na+/K+-ATPase. The LC50 value found was 8.07 mg/L. The results of sublethal exposure showed that TBARS levels decreased in liver and brain at every tested Zn concentration. In gills, there was an increase on TBARS levels at 1.0 mg/L and a decrease at 5.0 mg/L, while no changes in muscle were observed. Exposure to zinc decreased protein carbonyls in liver of silver catfish exposed to 5.0 mg/L and gills in every tested concentration. In brain there were no changes in protein carbonyl and in muscle there was an increase at both concentrations. In liver, the SOD activity increased at both zinc concentrations. CAT activity in liver did not change at any concentration. The GST activity decreased in liver and brain increased at both concentrations used. In gills, GST increased in fish exposed to 1.0 mg/L and in muscle at 5.0 mg/L. NPSH levels showed no changes in liver either in gills. In brain there was an increase on NPSH levels at 5.0 mg/L, while the muscle declined at both concentrations. The AChE activity in brain decreased at both zinc concentrations, whereas there was no change in muscle activity for this enzyme. The Na+/K+-ATPase activity was inhibited in gill and intestine in every used concentration. We may conclude that zinc altered biochemical parameters of silver catfish and also the inhibition of brain AChE and Na+/K+-ATPase activity in intestine and gills may be used as biomarkers of waters contaminated by zinc.A contaminação do ambiente aquático ocorre como consequência das atividades industriais, agrícolas e antropogênicas. Ecossistemas aquáticos são frequentemente contaminados com metais como cobre e zinco, que são essenciais em baixas concentrações, mas tóxicos em concentrações elevadas. Sendo assim, o objetivo deste estudo foi estabelecer a concentração letal média (CL50) em 96 horas do zinco para jundiá (Rhamdia quelen) e avaliar os possíveis efeitos sobre parâmetros bioquímicos destes peixes expostos a 0,0 (controle), 1,0 e 5,0 mg/L de zinco por 96h. Os parâmetros analisados foram substâncias reativas ao ácido tiobarbitúrico (TBARS), proteína carbonil, acetilcolinesterase (AChE), superóxido dismutase (SOD), catalase (CAT), glutationa S-transferase (GST), tióis não-proteicos (NPSH) e Na+/K+-ATPase. O valor da CL50 encontrado foi de 8,07 mg/L. Os resultados da exposição subletal mostraram que os níveis de TBARS diminuíram no fígado e no cérebro em todas concentrações de Zn testadas. Nas brânquias, houve um aumento dos níveis de TBARS na concentração de 1,0 mg/L e uma diminuição na 5,0 mg/L, enquanto que no músculo não foram observadas alterações. A exposição ao zinco diminuiu a carbonilação de proteínas no fígado de jundiás expostos a 5,0 mg/L e nas brânquias nas duas concentrações testadas. No cérebro não houve alterações na proteína carbonil e no músculo, houve um aumento nas duas concentrações. No fígado, a atividade da SOD aumentou em ambas as concentrações de zinco testadas. A atividade da CAT no fígado não teve alteração em nenhuma das concentrações. A atividade da GST diminuiu no fígado e aumentou no cérebro para ambas as concentrações utilizadas. Nas brânquias, o aumento da GST ocorreu na concentração de 1,0 mg/L e no músculo dos peixes expostos a 5,0 mg/L. Os níveis de NPSH não mostraram alterações no fígado e nem nas brânquias. No cérebro houve um aumento nos níveis de NPSH na concentração de 5,0 mg/L, enquanto que no músculo diminuiu nas duas concentrações. A atividade da AChE em cérebro diminuiu nas duas concentrações de zinco, enquanto que no músculo não houve alteração na atividade desta enzima. A atividade da Na+/K+-ATPase foi inibida em brânquias e intestino em todas as concentrações usadas. Com estes resultados, podemos concluir que o zinco alterou os parâmetros bioquímicos dos jundiás e que a inibição da AChE cerebral e da Na+/K+-ATPase no intestino e brânquias podem ser utilizadas como biomarcadores de águas contaminadas por zinco.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaConcentração letal médiaEstresse oxidativoJundiásMetalNa+/K+-ATPaseMediam lethal concentrationOxidative stressSilver catfishMetalNa+/K+-ATPase activityCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAParâmetros bioquímicos em jundiás expostos ao zincoBiochemical parameters in silver catfish exposed to zincinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisLoro, Vania Luciahttp://lattes.cnpq.br/6392817606416780Rosemberg, Denis Broockhttp://lattes.cnpq.br/7713953979203056Baldisserotto, Bernardohttp://lattes.cnpq.br/1036046601275319http://lattes.cnpq.br/4571079855289275Leitemperger, Jossiele Wesz200800000002400500300500500b0918185-c6b7-49df-b8d6-d245d73535b8f21ce45b-1d30-408a-9ea8-c459cf996b3dc677f64b-5cc5-4b69-a688-583d9209b1b0a64e0440-e917-4c31-ad62-51b4ea8f37fdinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALLEITEMPERGER, JOSSIELE WESZ.pdfapplication/pdf650533http://repositorio.ufsm.br/bitstream/1/11238/1/LEITEMPERGER%2c%20JOSSIELE%20WESZ.pdf640ecfc0d76732e748dcc28fdc32b674MD51TEXTLEITEMPERGER, JOSSIELE WESZ.pdf.txtLEITEMPERGER, JOSSIELE WESZ.pdf.txtExtracted texttext/plain74779http://repositorio.ufsm.br/bitstream/1/11238/2/LEITEMPERGER%2c%20JOSSIELE%20WESZ.pdf.txt6595d467757acb6cf782b9c634631273MD52THUMBNAILLEITEMPERGER, JOSSIELE WESZ.pdf.jpgLEITEMPERGER, JOSSIELE WESZ.pdf.jpgIM Thumbnailimage/jpeg4640http://repositorio.ufsm.br/bitstream/1/11238/3/LEITEMPERGER%2c%20JOSSIELE%20WESZ.pdf.jpg3bb798ef890ed46cbd70f646b7a1f510MD531/112382022-09-06 16:47:54.842oai:repositorio.ufsm.br:1/11238Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-09-06T19:47:54Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Parâmetros bioquímicos em jundiás expostos ao zinco |
dc.title.alternative.eng.fl_str_mv |
Biochemical parameters in silver catfish exposed to zinc |
title |
Parâmetros bioquímicos em jundiás expostos ao zinco |
spellingShingle |
Parâmetros bioquímicos em jundiás expostos ao zinco Leitemperger, Jossiele Wesz Concentração letal média Estresse oxidativo Jundiás Metal Na+/K+-ATPase Mediam lethal concentration Oxidative stress Silver catfish Metal Na+/K+-ATPase activity CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Parâmetros bioquímicos em jundiás expostos ao zinco |
title_full |
Parâmetros bioquímicos em jundiás expostos ao zinco |
title_fullStr |
Parâmetros bioquímicos em jundiás expostos ao zinco |
title_full_unstemmed |
Parâmetros bioquímicos em jundiás expostos ao zinco |
title_sort |
Parâmetros bioquímicos em jundiás expostos ao zinco |
author |
Leitemperger, Jossiele Wesz |
author_facet |
Leitemperger, Jossiele Wesz |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Loro, Vania Lucia |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6392817606416780 |
dc.contributor.referee1.fl_str_mv |
Rosemberg, Denis Broock |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7713953979203056 |
dc.contributor.referee2.fl_str_mv |
Baldisserotto, Bernardo |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1036046601275319 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4571079855289275 |
dc.contributor.author.fl_str_mv |
Leitemperger, Jossiele Wesz |
contributor_str_mv |
Loro, Vania Lucia Rosemberg, Denis Broock Baldisserotto, Bernardo |
dc.subject.por.fl_str_mv |
Concentração letal média Estresse oxidativo Jundiás Metal Na+/K+-ATPase |
topic |
Concentração letal média Estresse oxidativo Jundiás Metal Na+/K+-ATPase Mediam lethal concentration Oxidative stress Silver catfish Metal Na+/K+-ATPase activity CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Mediam lethal concentration Oxidative stress Silver catfish Metal Na+/K+-ATPase activity |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
The contamination of the aquatic environment occurs as a consequence of industrial, agricultural and anthropogenic activities. Aquatic ecosystems are often contaminated with metals such as copper and zinc, which are essential in low concentrations but toxic at high concentrations. Thus, the aim of this study was to establish the mean lethal concentration (LC50) within 96 hours of zinc for silver catfish (Rhamdia quelen) and to evaluate the possible effects on biochemical parameters of fish exposed to 0.0 (control), 1.0 and 5.0 mg/L zinc for 96h. The parameters analyzed were reactive substances to thiobarbituric acid (TBARS), protein carbonyl, acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), non-protein thiols (NPSH) and Na+/K+-ATPase. The LC50 value found was 8.07 mg/L. The results of sublethal exposure showed that TBARS levels decreased in liver and brain at every tested Zn concentration. In gills, there was an increase on TBARS levels at 1.0 mg/L and a decrease at 5.0 mg/L, while no changes in muscle were observed. Exposure to zinc decreased protein carbonyls in liver of silver catfish exposed to 5.0 mg/L and gills in every tested concentration. In brain there were no changes in protein carbonyl and in muscle there was an increase at both concentrations. In liver, the SOD activity increased at both zinc concentrations. CAT activity in liver did not change at any concentration. The GST activity decreased in liver and brain increased at both concentrations used. In gills, GST increased in fish exposed to 1.0 mg/L and in muscle at 5.0 mg/L. NPSH levels showed no changes in liver either in gills. In brain there was an increase on NPSH levels at 5.0 mg/L, while the muscle declined at both concentrations. The AChE activity in brain decreased at both zinc concentrations, whereas there was no change in muscle activity for this enzyme. The Na+/K+-ATPase activity was inhibited in gill and intestine in every used concentration. We may conclude that zinc altered biochemical parameters of silver catfish and also the inhibition of brain AChE and Na+/K+-ATPase activity in intestine and gills may be used as biomarkers of waters contaminated by zinc. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-06-05 |
dc.date.accessioned.fl_str_mv |
2015-11-04 |
dc.date.available.fl_str_mv |
2015-11-04 |
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info:eu-repo/semantics/publishedVersion |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
LEITEMPERGER, Jossiele Wesz. Biochemical parameters in silver catfish exposed to zinc. 2014. 46 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/11238 |
identifier_str_mv |
LEITEMPERGER, Jossiele Wesz. Biochemical parameters in silver catfish exposed to zinc. 2014. 46 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
url |
http://repositorio.ufsm.br/handle/1/11238 |
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