Efeito das poliaminas sobre a lipoperoxidação induzida por neurotoxinas em encéfalo de ratos

Detalhes bibliográficos
Ano de defesa: 2004
Autor(a) principal: Bellé, Nádia Aléssio Velloso lattes
Orientador(a): Rubin, Maribel Antonello lattes
Banca de defesa: Dutra Filho, Carlos Severo, Siqueira, Ionara Rodrigues
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
Departamento: Bioquímica
País: Brasil
Palavras-chave em Português:
Bioquímica
Poliaminas
Espermina
Espermidina
Antioxidantes
Sistema nervoso central
Cérebro
Área do conhecimento CNPq:
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
Link de acesso: http://repositorio.ufsm.br/handle/1/26801
Resumo: Polyamines, among other functions, are considered to act as a free radical scavenger and antioxidant. The quinolinic acid, sodium nitroprusside and iron stimulate production of free radicals and consequent lipid peroxidation, by diverses mechanisms. In the present study we investigated the effects of polyamines, spermine and spermidine, on thiobarbituric reactive species (TBARS) production – an index of lipid peroxidation – induced by quinolinic acid, sodium nitroprusside, iron/EDTA system and free iron in rat brain. Spermine and spermidine, in the minor concentrations tested (nM), did not had a significant effect on basal lipid peroxidation or on quinolinic acid-induced lipid peroxidation. Nevertheless both polyamines, in micromolar concentrations, inhibited quinolinic acid-induced TBARS production; being spermine more efficient than spermidine. Spermine and spermidine also inhibited sodium nitroprusside-, iron/EDTA- and free iron-induced lipid peroxidation, but had a modest effect and only in high concentrations (mM). In addiction, in the presence of MK-801, quinolinic acid-induced lipid peroxidation was considerably more inhibited by polyamines. Arcaine did not affect the reducer effect of polyamines. The present findings suggest that the observed effects of polyamines are not related to the activation of NMDA receptor but with their free radical scavenger properties.
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spelling 2022-11-08T17:54:05Z2022-11-08T17:54:05Z2004-01-16http://repositorio.ufsm.br/handle/1/26801Polyamines, among other functions, are considered to act as a free radical scavenger and antioxidant. The quinolinic acid, sodium nitroprusside and iron stimulate production of free radicals and consequent lipid peroxidation, by diverses mechanisms. In the present study we investigated the effects of polyamines, spermine and spermidine, on thiobarbituric reactive species (TBARS) production – an index of lipid peroxidation – induced by quinolinic acid, sodium nitroprusside, iron/EDTA system and free iron in rat brain. Spermine and spermidine, in the minor concentrations tested (nM), did not had a significant effect on basal lipid peroxidation or on quinolinic acid-induced lipid peroxidation. Nevertheless both polyamines, in micromolar concentrations, inhibited quinolinic acid-induced TBARS production; being spermine more efficient than spermidine. Spermine and spermidine also inhibited sodium nitroprusside-, iron/EDTA- and free iron-induced lipid peroxidation, but had a modest effect and only in high concentrations (mM). In addiction, in the presence of MK-801, quinolinic acid-induced lipid peroxidation was considerably more inhibited by polyamines. Arcaine did not affect the reducer effect of polyamines. The present findings suggest that the observed effects of polyamines are not related to the activation of NMDA receptor but with their free radical scavenger properties.As poliaminas, entre suas variadas funções, podem atuar também como antioxidantes bem como removedoras de radicais livres. O ácido quinolínico, nitroprussiato de sódio e ferro estimulam a produção de radicais livres e conseqüente lipoperoxidação, através de mecanismos variados. No presente estudo nós investigamos os efeitos das poliaminas, espermina e espermidina, sobre a produção de espécies reativas ao ácido tiobarbitúrico (TBARS) – um indicativo de lipoperoxidação – induzida por ácido quinolínico, nitroprussiato de sódio, sistema ferro/EDTA e ferro livre em encéfalos de ratos. Espermina e espermidina, nas menores concentrações testadas (nM), não tiveram efeito significativo sobre a lipoperoxidação basal ou sobre a lipoperoxidação induzida por ácido quinolínico. No entanto ambas poliaminas, a concentrações micromolares, inibiram a produção de TBARS induzida por ácido quinolínico; sendo que a espermina foi mais eficaz que a espermidina. Espermina e espermidina também inibiram a lipoperoxidação induzida por nitroprussiato de sódio, ferro/EDTA e ferro livre; contudo obtiveram um modesto efeito e somente em altas concentrações (mM). Em adição, ambas poliaminas tiveram seu efeito inibitório acentuado pelo MK-801 quando a lipoperoxidação foi induzida pelo ácido quinolínico. Já a arcaína não alterou o efeito inibitório das poliaminas. Os presentes achados sugerem que os efeitos observados para as poliaminas não estão relacionados à sua atividade sobre o receptor NMDA mas com suas propriedades removedoras de radicais livres.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessBioquímicaPoliaminasEsperminaEspermidinaAntioxidantesSistema nervoso centralCérebroCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEfeito das poliaminas sobre a lipoperoxidação induzida por neurotoxinas em encéfalo de ratosEffect of polyamines on lipid peroxidation induced by neurotoxins in rat brain.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisRubin, Maribel Antonellohttp://lattes.cnpq.br/7237734243628134Rocha, Joao Batista Teixeira daDutra Filho, Carlos SeveroSiqueira, Ionara Rodrigueshttp://lattes.cnpq.br/7692247567521461Bellé, Nádia Aléssio Velloso20080000000260060060060060060017f72a93-232f-40b3-9c14-1b54b978789b291333fa-7fb5-475f-b4e5-3c96c8a548e114d04cad-6929-4697-a8d6-d1303391d2183cccc308-bad2-4388-bafc-3020582105987957cf8a-198b-41b4-984b-a20a1800e9c6reponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/26801/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52ORIGINALDIS_PPGCBBT_2004_BELLÉ_NADIA.pdfDIS_PPGCBBT_2004_BELLÉ_NADIA.pdfDissertação de Mestradoapplication/pdf393894http://repositorio.ufsm.br/bitstream/1/26801/1/DIS_PPGCBBT_2004_BELL%c3%89_NADIA.pdf3b9768af636d0eed4904900a269d9c1aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/26801/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD531/268012022-11-08 14:54:05.908oai:repositorio.ufsm.br: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ório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-11-08T17:54:05Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.por.fl_str_mv Efeito das poliaminas sobre a lipoperoxidação induzida por neurotoxinas em encéfalo de ratos
dc.title.alternative.eng.fl_str_mv Effect of polyamines on lipid peroxidation induced by neurotoxins in rat brain.
title Efeito das poliaminas sobre a lipoperoxidação induzida por neurotoxinas em encéfalo de ratos
spellingShingle Efeito das poliaminas sobre a lipoperoxidação induzida por neurotoxinas em encéfalo de ratos
Bellé, Nádia Aléssio Velloso
Bioquímica
Poliaminas
Espermina
Espermidina
Antioxidantes
Sistema nervoso central
Cérebro
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short Efeito das poliaminas sobre a lipoperoxidação induzida por neurotoxinas em encéfalo de ratos
title_full Efeito das poliaminas sobre a lipoperoxidação induzida por neurotoxinas em encéfalo de ratos
title_fullStr Efeito das poliaminas sobre a lipoperoxidação induzida por neurotoxinas em encéfalo de ratos
title_full_unstemmed Efeito das poliaminas sobre a lipoperoxidação induzida por neurotoxinas em encéfalo de ratos
title_sort Efeito das poliaminas sobre a lipoperoxidação induzida por neurotoxinas em encéfalo de ratos
author Bellé, Nádia Aléssio Velloso
author_facet Bellé, Nádia Aléssio Velloso
author_role author
dc.contributor.advisor1.fl_str_mv Rubin, Maribel Antonello
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/7237734243628134
dc.contributor.advisor-co1.fl_str_mv Rocha, Joao Batista Teixeira da
dc.contributor.referee1.fl_str_mv Dutra Filho, Carlos Severo
dc.contributor.referee2.fl_str_mv Siqueira, Ionara Rodrigues
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7692247567521461
dc.contributor.author.fl_str_mv Bellé, Nádia Aléssio Velloso
contributor_str_mv Rubin, Maribel Antonello
Rocha, Joao Batista Teixeira da
Dutra Filho, Carlos Severo
Siqueira, Ionara Rodrigues
dc.subject.por.fl_str_mv Bioquímica
Poliaminas
Espermina
Espermidina
Antioxidantes
Sistema nervoso central
Cérebro
topic Bioquímica
Poliaminas
Espermina
Espermidina
Antioxidantes
Sistema nervoso central
Cérebro
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
description Polyamines, among other functions, are considered to act as a free radical scavenger and antioxidant. The quinolinic acid, sodium nitroprusside and iron stimulate production of free radicals and consequent lipid peroxidation, by diverses mechanisms. In the present study we investigated the effects of polyamines, spermine and spermidine, on thiobarbituric reactive species (TBARS) production – an index of lipid peroxidation – induced by quinolinic acid, sodium nitroprusside, iron/EDTA system and free iron in rat brain. Spermine and spermidine, in the minor concentrations tested (nM), did not had a significant effect on basal lipid peroxidation or on quinolinic acid-induced lipid peroxidation. Nevertheless both polyamines, in micromolar concentrations, inhibited quinolinic acid-induced TBARS production; being spermine more efficient than spermidine. Spermine and spermidine also inhibited sodium nitroprusside-, iron/EDTA- and free iron-induced lipid peroxidation, but had a modest effect and only in high concentrations (mM). In addiction, in the presence of MK-801, quinolinic acid-induced lipid peroxidation was considerably more inhibited by polyamines. Arcaine did not affect the reducer effect of polyamines. The present findings suggest that the observed effects of polyamines are not related to the activation of NMDA receptor but with their free radical scavenger properties.
publishDate 2004
dc.date.issued.fl_str_mv 2004-01-16
dc.date.accessioned.fl_str_mv 2022-11-08T17:54:05Z
dc.date.available.fl_str_mv 2022-11-08T17:54:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/26801
url http://repositorio.ufsm.br/handle/1/26801
dc.language.iso.fl_str_mv por
language por
dc.relation.cnpq.fl_str_mv 200800000002
dc.relation.confidence.fl_str_mv 600
600
600
600
600
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dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
dc.publisher.initials.fl_str_mv UFSM
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Bioquímica
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
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