O disseleneto de difenila protege o modelo Caenorhabditis elegans para a doença de huntington através da ativação antioxidantes reduzindo a agregação de proteínas

Detalhes bibliográficos
Ano de defesa: 2020
Autor(a) principal: Baptista, Fabiane Bicca Obetine lattes
Orientador(a): Soares, Félix Alexandre Antunes lattes
Banca de defesa: Ávila, Daiana Silva de, Rosemberg , Denis Broock
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
Departamento: Bioquímica
País: Brasil
Palavras-chave em Português:
Proteínas
Doenças neurodegenerativas
Sequência de poliglutamina
Selênio
Área do conhecimento CNPq:
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
Link de acesso: http://repositorio.ufsm.br/handle/1/22031
Resumo: Huntington's disease (HD) is a progressive, autosomal dominant neurodegenerative disease with a distinct phenotype, including chorea and dystonia, incoordination, cognitive decline and behavioral difficulties. Characterized by the presence of the mutant huntingtin protein, which results from an expanded CAG repeat, leading to a variable length polyglutamine (Poli-Q) chain at the N-terminal. Most neurodegenerative diseases are characterized by abnormal deposition and consequent protein aggregation, which impair the dynamics of protein networks and result in the imbalance of cellular homeostasis. In this work, we used the Caenorhabditis elegans experimental model due to its easy manipulation and high homology of genes and signaling pathways in relation to mammals. The worms were exposed to diphenyl diselenide (PhSe)2 at concentrations of 25, 50 and 100 μM, from young adults and analyzed for poly-Q aggregation and neuronal and muscle viability. Based on the theory of aging and the aggregation of proteins related to HD, and taking into account that (PhSe)2 has antioxidant activity, then we will analyze some possible pathways involved related to HD), we will also analyze as to its potential in increasing the quality and life span of C. elegans.
id UFSM-20_c96c77fe90a1a243dac18a958882916e
oai_identifier_str oai:repositorio.ufsm.br:1/22031
network_acronym_str UFSM-20
network_name_str Manancial - Repositório Digital da UFSM
repository_id_str
spelling 2021-08-23T19:49:26Z2021-08-23T19:49:26Z2020-06-16http://repositorio.ufsm.br/handle/1/22031Huntington's disease (HD) is a progressive, autosomal dominant neurodegenerative disease with a distinct phenotype, including chorea and dystonia, incoordination, cognitive decline and behavioral difficulties. Characterized by the presence of the mutant huntingtin protein, which results from an expanded CAG repeat, leading to a variable length polyglutamine (Poli-Q) chain at the N-terminal. Most neurodegenerative diseases are characterized by abnormal deposition and consequent protein aggregation, which impair the dynamics of protein networks and result in the imbalance of cellular homeostasis. In this work, we used the Caenorhabditis elegans experimental model due to its easy manipulation and high homology of genes and signaling pathways in relation to mammals. The worms were exposed to diphenyl diselenide (PhSe)2 at concentrations of 25, 50 and 100 μM, from young adults and analyzed for poly-Q aggregation and neuronal and muscle viability. Based on the theory of aging and the aggregation of proteins related to HD, and taking into account that (PhSe)2 has antioxidant activity, then we will analyze some possible pathways involved related to HD), we will also analyze as to its potential in increasing the quality and life span of C. elegans.A doença de Huntington (DH) é uma doença neurodegenerativa progressiva, autossômica dominante, com um fenótipo distinto, incluindo coreia e distonia, descoordenação, declínio cognitivo e dificuldades comportamentais. Caracterizada pela presença da proteína mutante huntingtina, que resulta de uma repetição da sequência de nucleotídeos (CAG) expandida, conduzindo a uma cadeia de poliglutamina (Poli-Q) de comprimento variável no terminal N. A maioria das doenças neurodegenerativas são caracterizadas pela deposição anormal e consequente agregação de proteínas, que prejudicam a dinâmica das redes proteicas e resultam no desequilíbrio da homeostase celular. Neste trabalho, utilizamos o Caenorhabditis elegans devido a sua fácil manipulação e alta homologia de genes e vias de sinalização em relação aos mamíferos. Os vermes foram expostos ao disseleneto de difenila (PhSe)2 nas concentrações de 25, 50 e 100 μM, a partir de adultos jovens e analisados quanto à agregação da poli-Q e viabilidade neuronal e muscular. Baseando-se na teoria de envelhecimento e na agregação de proteínas relacionadas com a DH, e levando em consideração que o (PhSe)2 possui atividade antioxidante, analisamos as possíveis vias envolvidas e relacionada com DH, também analisamos quanto ao seu potencial em aumentar a qualidade e o tempo de vida de C. elegans.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessProteínasDoenças neurodegenerativasSequência de poliglutaminaSelênioCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAO disseleneto de difenila protege o modelo Caenorhabditis elegans para a doença de huntington através da ativação antioxidantes reduzindo a agregação de proteínasDiphenyl diselenide protects the model Caenorhabditis elegans for huntington's disease by activating antioxidants reducing protein aggregationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSoares, Félix Alexandre Antuneshttp://lattes.cnpq.br/8752453650114092Ávila, Daiana Silva deRosemberg , Denis Broockhttp://lattes.cnpq.br/2341781666863236Baptista, Fabiane Bicca Obetine2008000000026006006006006003aef53b7-b173-4c56-a944-bed2de5e5dda97f06430-9939-4e42-b65a-3ad639bcfc139f9969d1-7d48-45fd-9ea7-9a55acc6fc288ade1f91-8aa4-433a-8948-aa845c5ce69dreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGCBBT_2020_BAPTISTA_FABIANE.pdfDIS_PPGCBBT_2020_BAPTISTA_FABIANE.pdfDissertaçãoapplication/pdf6733922http://repositorio.ufsm.br/bitstream/1/22031/1/DIS_PPGCBBT_2020_BAPTISTA_FABIANE.pdf61b7320ffc7008c47f882b5a6a357ebeMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/22031/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/22031/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53TEXTDIS_PPGCBBT_2020_BAPTISTA_FABIANE.pdf.txtDIS_PPGCBBT_2020_BAPTISTA_FABIANE.pdf.txtExtracted texttext/plain90371http://repositorio.ufsm.br/bitstream/1/22031/4/DIS_PPGCBBT_2020_BAPTISTA_FABIANE.pdf.txt0519dbce514900873be33f9235eeb75eMD54THUMBNAILDIS_PPGCBBT_2020_BAPTISTA_FABIANE.pdf.jpgDIS_PPGCBBT_2020_BAPTISTA_FABIANE.pdf.jpgIM Thumbnailimage/jpeg2415http://repositorio.ufsm.br/bitstream/1/22031/5/DIS_PPGCBBT_2020_BAPTISTA_FABIANE.pdf.jpg9e97de9c603799b5df34916acaa1e4c4MD551/220312021-08-24 03:00:48.329oai:repositorio.ufsm.br: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ório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132021-08-24T06:00:48Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.por.fl_str_mv O disseleneto de difenila protege o modelo Caenorhabditis elegans para a doença de huntington através da ativação antioxidantes reduzindo a agregação de proteínas
dc.title.alternative.eng.fl_str_mv Diphenyl diselenide protects the model Caenorhabditis elegans for huntington's disease by activating antioxidants reducing protein aggregation
title O disseleneto de difenila protege o modelo Caenorhabditis elegans para a doença de huntington através da ativação antioxidantes reduzindo a agregação de proteínas
spellingShingle O disseleneto de difenila protege o modelo Caenorhabditis elegans para a doença de huntington através da ativação antioxidantes reduzindo a agregação de proteínas
Baptista, Fabiane Bicca Obetine
Proteínas
Doenças neurodegenerativas
Sequência de poliglutamina
Selênio
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short O disseleneto de difenila protege o modelo Caenorhabditis elegans para a doença de huntington através da ativação antioxidantes reduzindo a agregação de proteínas
title_full O disseleneto de difenila protege o modelo Caenorhabditis elegans para a doença de huntington através da ativação antioxidantes reduzindo a agregação de proteínas
title_fullStr O disseleneto de difenila protege o modelo Caenorhabditis elegans para a doença de huntington através da ativação antioxidantes reduzindo a agregação de proteínas
title_full_unstemmed O disseleneto de difenila protege o modelo Caenorhabditis elegans para a doença de huntington através da ativação antioxidantes reduzindo a agregação de proteínas
title_sort O disseleneto de difenila protege o modelo Caenorhabditis elegans para a doença de huntington através da ativação antioxidantes reduzindo a agregação de proteínas
author Baptista, Fabiane Bicca Obetine
author_facet Baptista, Fabiane Bicca Obetine
author_role author
dc.contributor.advisor1.fl_str_mv Soares, Félix Alexandre Antunes
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8752453650114092
dc.contributor.referee1.fl_str_mv Ávila, Daiana Silva de
dc.contributor.referee2.fl_str_mv Rosemberg , Denis Broock
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2341781666863236
dc.contributor.author.fl_str_mv Baptista, Fabiane Bicca Obetine
contributor_str_mv Soares, Félix Alexandre Antunes
Ávila, Daiana Silva de
Rosemberg , Denis Broock
dc.subject.por.fl_str_mv Proteínas
Doenças neurodegenerativas
Sequência de poliglutamina
Selênio
topic Proteínas
Doenças neurodegenerativas
Sequência de poliglutamina
Selênio
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
description Huntington's disease (HD) is a progressive, autosomal dominant neurodegenerative disease with a distinct phenotype, including chorea and dystonia, incoordination, cognitive decline and behavioral difficulties. Characterized by the presence of the mutant huntingtin protein, which results from an expanded CAG repeat, leading to a variable length polyglutamine (Poli-Q) chain at the N-terminal. Most neurodegenerative diseases are characterized by abnormal deposition and consequent protein aggregation, which impair the dynamics of protein networks and result in the imbalance of cellular homeostasis. In this work, we used the Caenorhabditis elegans experimental model due to its easy manipulation and high homology of genes and signaling pathways in relation to mammals. The worms were exposed to diphenyl diselenide (PhSe)2 at concentrations of 25, 50 and 100 μM, from young adults and analyzed for poly-Q aggregation and neuronal and muscle viability. Based on the theory of aging and the aggregation of proteins related to HD, and taking into account that (PhSe)2 has antioxidant activity, then we will analyze some possible pathways involved related to HD), we will also analyze as to its potential in increasing the quality and life span of C. elegans.
publishDate 2020
dc.date.issued.fl_str_mv 2020-06-16
dc.date.accessioned.fl_str_mv 2021-08-23T19:49:26Z
dc.date.available.fl_str_mv 2021-08-23T19:49:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/22031
url http://repositorio.ufsm.br/handle/1/22031
dc.language.iso.fl_str_mv por
language por
dc.relation.cnpq.fl_str_mv 200800000002
dc.relation.confidence.fl_str_mv 600
600
600
600
600
dc.relation.authority.fl_str_mv 3aef53b7-b173-4c56-a944-bed2de5e5dda
97f06430-9939-4e42-b65a-3ad639bcfc13
9f9969d1-7d48-45fd-9ea7-9a55acc6fc28
8ade1f91-8aa4-433a-8948-aa845c5ce69d
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
dc.publisher.initials.fl_str_mv UFSM
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Bioquímica
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
bitstream.url.fl_str_mv http://repositorio.ufsm.br/bitstream/1/22031/1/DIS_PPGCBBT_2020_BAPTISTA_FABIANE.pdf
http://repositorio.ufsm.br/bitstream/1/22031/2/license_rdf
http://repositorio.ufsm.br/bitstream/1/22031/3/license.txt
http://repositorio.ufsm.br/bitstream/1/22031/4/DIS_PPGCBBT_2020_BAPTISTA_FABIANE.pdf.txt
http://repositorio.ufsm.br/bitstream/1/22031/5/DIS_PPGCBBT_2020_BAPTISTA_FABIANE.pdf.jpg
bitstream.checksum.fl_str_mv 61b7320ffc7008c47f882b5a6a357ebe
4460e5956bc1d1639be9ae6146a50347
2f0571ecee68693bd5cd3f17c1e075df
0519dbce514900873be33f9235eeb75e
9e97de9c603799b5df34916acaa1e4c4
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv
_version_ 1801223967228297216

Registros relacionados