Efeito da suplementação de carotenoides do urucum sobre a resistência da LDL-colesterol à oxidação e o status oxidativo em indivíduos saudáveis
Ano de defesa: | 2018 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências da Saúde |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Farmacologia
|
Departamento: |
Farmacologia
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/20223 |
Resumo: | Cardiovascular diseases are a group of varied diseases, including obstructive diseases, such coronary artery disease and stroke, which are the leading causes of death in the world. These obstructive events are consequence of a process, known as atherosclerosis, characterized by the accumulation of fibrous and lipid material in the arterial wall of large and medium-sized vessels. Atherosclerosis development involves several processes, among them lipids accumulation, endothelial dysfunction, inflammation and, especially, oxidative stress, which is responsible of low-density lipoprotein (LDL) oxidation. Thus, researchers have found alternatives to prevent atherosclerosis development and progression, such the consumption of antioxidant-rich food. Epidemiological studies have shown that higher serum levels of some carotenoids, such lycopene, are related to a lower cardiovascular risk. Despite this, some studies have questioned lycopene supplementation benefits. Therefore, researches have highlighted other carotenoids in the last few years. Among them, the carotenoids present in annatto seeds (Bixa orellana), bixin and norbixin, whose antioxidant and antiatherogenic potential has already been demonstrated by in vitro and animal studies. However, there are no reports in literature about its short and long-term consumption effects in humans. Therefore, the objective of this study was to evaluate the effects of bixin, norbixin and lycopene consumption for 7 days on biochemical and oxidative stress biomarkers, as well as the susceptibility of LDL to copper-induced oxidation in healthy individuals. A randomized, double blind, placebo-controlled, cross-over study were made with 16 healthy volunteers (8 men and 8 women), who received each one of the four treatments (bixin, norbixin, lycopene or placebo), at dose of 0.05 mg / kg body weight orally. The biochemical and oxidative stress parameters analysed were not affected by any treatment. However, in comparison to placebo, bixin supplementation reduced the oxidation rate of the lipid moiety of LDL and serum levels of nitric oxide metabolites (NOx). Besides, the positive correlation found between NOx levels and the susceptibility of LDL to ex-vivo oxidation, suggesting that the reduction of NOx levels could be the mechanism responsible for the protective action of bixin. These results suggest that bixin has a biological action that may help in atherosclerosis prevention. |
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2020-12-16T13:49:49Z2020-12-16T13:49:49Z2018-12-06http://repositorio.ufsm.br/handle/1/20223Cardiovascular diseases are a group of varied diseases, including obstructive diseases, such coronary artery disease and stroke, which are the leading causes of death in the world. These obstructive events are consequence of a process, known as atherosclerosis, characterized by the accumulation of fibrous and lipid material in the arterial wall of large and medium-sized vessels. Atherosclerosis development involves several processes, among them lipids accumulation, endothelial dysfunction, inflammation and, especially, oxidative stress, which is responsible of low-density lipoprotein (LDL) oxidation. Thus, researchers have found alternatives to prevent atherosclerosis development and progression, such the consumption of antioxidant-rich food. Epidemiological studies have shown that higher serum levels of some carotenoids, such lycopene, are related to a lower cardiovascular risk. Despite this, some studies have questioned lycopene supplementation benefits. Therefore, researches have highlighted other carotenoids in the last few years. Among them, the carotenoids present in annatto seeds (Bixa orellana), bixin and norbixin, whose antioxidant and antiatherogenic potential has already been demonstrated by in vitro and animal studies. However, there are no reports in literature about its short and long-term consumption effects in humans. Therefore, the objective of this study was to evaluate the effects of bixin, norbixin and lycopene consumption for 7 days on biochemical and oxidative stress biomarkers, as well as the susceptibility of LDL to copper-induced oxidation in healthy individuals. A randomized, double blind, placebo-controlled, cross-over study were made with 16 healthy volunteers (8 men and 8 women), who received each one of the four treatments (bixin, norbixin, lycopene or placebo), at dose of 0.05 mg / kg body weight orally. The biochemical and oxidative stress parameters analysed were not affected by any treatment. However, in comparison to placebo, bixin supplementation reduced the oxidation rate of the lipid moiety of LDL and serum levels of nitric oxide metabolites (NOx). Besides, the positive correlation found between NOx levels and the susceptibility of LDL to ex-vivo oxidation, suggesting that the reduction of NOx levels could be the mechanism responsible for the protective action of bixin. These results suggest that bixin has a biological action that may help in atherosclerosis prevention.As doenças cardiovasculares são um grupo de doenças variadas, que incluem doenças de natureza obstrutiva, como a doença arterial coronariana e o acidente vascular cerebral, que constituem as principais causas de morte no mundo. A ocorrência desses eventos obstrutivos é consequência de uma processo conhecido como aterosclerose, que caracteriza-se pelo acúmulo de material lipídico e fibroso na parede arterial de vasos de grande e médio calibre. O desenvolvimento da aterosclerose envolve diversos processos, dentre eles o acúmulo vascular de lipídios, a disfunção endotelial, a inflamação e, especialmente, o estresse oxidativo, que é responsável pela oxidação da lipoproteína de baixa densidade (LDL). Dessa forma, pesquisadores têm buscado alternativas para evitar o desenvolvimento e a progressão da aterosclerose, como o consumo de alimentos ricos em compostos antioxidantes. Estudos epidemiológicos mostram que maiores níveis séricos de alguns carotenoides, como o licopeno, estão relacionados a um menor risco de sofrer um evento cardiovascular. Apesar disso, alguns estudos têm questionado os benefícios associados à suplementação com o licopeno. Sendo assim, outros carotenoides têm sido foco de pesquisas nos últimos anos. Dentre eles, os carotenoides presentes nas sementes do urucum (Bixa orellana), bixina e norbixina, cujo potencial antioxidante e antiaterogênico já foi demonstrado por estudos in vitro e em modelos animais. No entanto, não há relatos na literatura sobre os efeitos do seu consumo à curto e longo prazo por humanos. Logo, o objetivo deste estudo foi avaliar os efeitos do consumo de bixina, norbixina e licopeno durante 7 dias sobre biomarcadores bioquímicos e de estresse oxidativo, além da susceptibilidade da LDL à oxidação induzida por cobre em indivíduos saudáveis. Para tal, foi realizado um ensaio clínico randomizado, duplo cego, controlado por placebo e com delineamento cruzado com retorno. Participaram do estudo 16 voluntários saudáveis (8 homens e 8 mulheres), que receberam por via oral cada um dos quatro tratamentos propostos (bixina, norbixina, licopeno ou placebo) na dose de 0,05 mg/kg de peso corporal. Os marcadores bioquímicos e de estresse oxidativo avaliados, não foram afetados por nenhum dos tratamentos analisados. No entanto, em comparação ao placebo, a suplementação com bixina reduziu a taxa de oxidação da fração lipídica da LDL e os níveis séricos dos metabolitos do óxido nítrico (NOx). Além disso, houve uma correlação positiva entre os níveis de NOx e a suscetibilidade da LDL a oxidação ex-vivo, sugerindo que a redução dos níveis de NOx parece ser o mecanismo responsável pela ação protetora da bixina. Estes resultados sugerem que a bixina possui uma ação biológica que pode auxiliar na prevenção da aterosclerose.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências da SaúdePrograma de Pós-Graduação em FarmacologiaUFSMBrasilFarmacologiaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAteroscleroseOxidação da LDLNOxCarotenoideUrucumAtherosclerosisLDL oxidationCarotenoidAnnattoCNPQ::CIENCIAS DA SAUDE::FARMACIAEfeito da suplementação de carotenoides do urucum sobre a resistência da LDL-colesterol à oxidação e o status oxidativo em indivíduos saudáveisEffect of annatto carotenoids supplementation on LDL-cholesterol resistance to oxidation and oxidative status in healthy subjectsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisEmanuelli, Tatianahttp://lattes.cnpq.br/2165391096880394Somacal, Sabrinahttp://lattes.cnpq.br/2034988951511299Bochi, Guilherme Vargashttp://lattes.cnpq.br/4191221572795869Conterato, Greicy Michelle Marafigahttp://lattes.cnpq.br/2608761450046726http://lattes.cnpq.br/1874717751481173Conte, Lisiane4003000000056007812e191-0e91-4b97-bc2b-b442502f01864944856e-6154-4cfa-9be8-fdc073a76b21db78b1f3-a3f4-4c5d-8d55-a95d19e8eaf26e2ef066-1dbd-42a0-9cf7-367e79b503545ab138d2-d6c9-41f5-be1a-bff2b1d3b3ecreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGFARMACOLOGIA_2018_CONTE_LISIANE.pdfDIS_PPGFARMACOLOGIA_2018_CONTE_LISIANE.pdfDissertação de Mestradoapplication/pdf2405201http://repositorio.ufsm.br/bitstream/1/20223/1/DIS_PPGFARMACOLOGIA_2018_CONTE_LISIANE.pdf2951a667bbd8cb52253d72c26ac09834MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Efeito da suplementação de carotenoides do urucum sobre a resistência da LDL-colesterol à oxidação e o status oxidativo em indivíduos saudáveis |
dc.title.alternative.eng.fl_str_mv |
Effect of annatto carotenoids supplementation on LDL-cholesterol resistance to oxidation and oxidative status in healthy subjects |
title |
Efeito da suplementação de carotenoides do urucum sobre a resistência da LDL-colesterol à oxidação e o status oxidativo em indivíduos saudáveis |
spellingShingle |
Efeito da suplementação de carotenoides do urucum sobre a resistência da LDL-colesterol à oxidação e o status oxidativo em indivíduos saudáveis Conte, Lisiane Aterosclerose Oxidação da LDL NOx Carotenoide Urucum Atherosclerosis LDL oxidation Carotenoid Annatto CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Efeito da suplementação de carotenoides do urucum sobre a resistência da LDL-colesterol à oxidação e o status oxidativo em indivíduos saudáveis |
title_full |
Efeito da suplementação de carotenoides do urucum sobre a resistência da LDL-colesterol à oxidação e o status oxidativo em indivíduos saudáveis |
title_fullStr |
Efeito da suplementação de carotenoides do urucum sobre a resistência da LDL-colesterol à oxidação e o status oxidativo em indivíduos saudáveis |
title_full_unstemmed |
Efeito da suplementação de carotenoides do urucum sobre a resistência da LDL-colesterol à oxidação e o status oxidativo em indivíduos saudáveis |
title_sort |
Efeito da suplementação de carotenoides do urucum sobre a resistência da LDL-colesterol à oxidação e o status oxidativo em indivíduos saudáveis |
author |
Conte, Lisiane |
author_facet |
Conte, Lisiane |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Emanuelli, Tatiana |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2165391096880394 |
dc.contributor.advisor-co1.fl_str_mv |
Somacal, Sabrina |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/2034988951511299 |
dc.contributor.referee1.fl_str_mv |
Bochi, Guilherme Vargas |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/4191221572795869 |
dc.contributor.referee2.fl_str_mv |
Conterato, Greicy Michelle Marafiga |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/2608761450046726 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1874717751481173 |
dc.contributor.author.fl_str_mv |
Conte, Lisiane |
contributor_str_mv |
Emanuelli, Tatiana Somacal, Sabrina Bochi, Guilherme Vargas Conterato, Greicy Michelle Marafiga |
dc.subject.por.fl_str_mv |
Aterosclerose Oxidação da LDL NOx Carotenoide Urucum |
topic |
Aterosclerose Oxidação da LDL NOx Carotenoide Urucum Atherosclerosis LDL oxidation Carotenoid Annatto CNPQ::CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Atherosclerosis LDL oxidation Carotenoid Annatto |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
Cardiovascular diseases are a group of varied diseases, including obstructive diseases, such coronary artery disease and stroke, which are the leading causes of death in the world. These obstructive events are consequence of a process, known as atherosclerosis, characterized by the accumulation of fibrous and lipid material in the arterial wall of large and medium-sized vessels. Atherosclerosis development involves several processes, among them lipids accumulation, endothelial dysfunction, inflammation and, especially, oxidative stress, which is responsible of low-density lipoprotein (LDL) oxidation. Thus, researchers have found alternatives to prevent atherosclerosis development and progression, such the consumption of antioxidant-rich food. Epidemiological studies have shown that higher serum levels of some carotenoids, such lycopene, are related to a lower cardiovascular risk. Despite this, some studies have questioned lycopene supplementation benefits. Therefore, researches have highlighted other carotenoids in the last few years. Among them, the carotenoids present in annatto seeds (Bixa orellana), bixin and norbixin, whose antioxidant and antiatherogenic potential has already been demonstrated by in vitro and animal studies. However, there are no reports in literature about its short and long-term consumption effects in humans. Therefore, the objective of this study was to evaluate the effects of bixin, norbixin and lycopene consumption for 7 days on biochemical and oxidative stress biomarkers, as well as the susceptibility of LDL to copper-induced oxidation in healthy individuals. A randomized, double blind, placebo-controlled, cross-over study were made with 16 healthy volunteers (8 men and 8 women), who received each one of the four treatments (bixin, norbixin, lycopene or placebo), at dose of 0.05 mg / kg body weight orally. The biochemical and oxidative stress parameters analysed were not affected by any treatment. However, in comparison to placebo, bixin supplementation reduced the oxidation rate of the lipid moiety of LDL and serum levels of nitric oxide metabolites (NOx). Besides, the positive correlation found between NOx levels and the susceptibility of LDL to ex-vivo oxidation, suggesting that the reduction of NOx levels could be the mechanism responsible for the protective action of bixin. These results suggest that bixin has a biological action that may help in atherosclerosis prevention. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-12-06 |
dc.date.accessioned.fl_str_mv |
2020-12-16T13:49:49Z |
dc.date.available.fl_str_mv |
2020-12-16T13:49:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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http://repositorio.ufsm.br/handle/1/20223 |
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http://repositorio.ufsm.br/handle/1/20223 |
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por |
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por |
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400300000005 |
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600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Farmacologia |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Farmacologia |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.source.none.fl_str_mv |
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