Desenvolvimento de oócitos bovinos mantidos em líquido folicular
Ano de defesa: | 2001 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Rurais |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Medicina Veterinária
|
Departamento: |
Medicina Veterinária
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/26723 |
Resumo: | To evaluate the effect of maintenance of bovine oocytes in follicular fluid (LF) for in vitro production of embryos, 1322 Complex Cumulus Oophorus (CCO's) obtained from abattoir bovine ovaries were distributed in two experiments. In the experiment I, maturation performed in TCM-199 for 24 hours (Control), was compared with the maintenance in follicular fluid at 30ºC for two (LF2), four (LF4) and six hours (LF6), followed by complementary TCM-199 maturation for 22, 20 and 18 hours, respectively. In the experiment II, oocyte maturation in TCM-199 for 24 hours (Control) and 18 hours (M18), was compared with the maintenance in LF for 6 hours followed by 18 hours TCM-199 maturation (LF6+M18). In both experiments, after maturation, the oocytes were transferred to Fert-Talp medium added of heparin and fertilization performed with semen selected by swim-up procedure. After that, zygotes were denuded and cultured in SOFaa medium. In the experiment I, the cleavage rate observed was 81.6% in Control group, 80.0% in LF2 group, 80.7% in LF4 group, and 81.7% in LF6 group. The blastocyst rate in D9 was 22.3%, 19.4%, 24.4% and 26.7% for Control group, LF2, LF4 and LF6 groups, respectively. No differences were observed (p>0.05). In experiment II, the cleavage rates (79.8%, 76.2% and 84.9%) and day 9 blastocyst rates (26.4%, 27.7% and 35.4%) in Control group, M18 and LF6+M18 groups, respectively, were not significantly different (p>0.05). The results obtained suggest that it is possible to maintain bovine oocytes in fluid obtained from follicles with 2 to 8mm of diameter, for periods until 6 hours at 30ºC, resulting in a simple, practical, and efficient alternative for maintenance and transport of bovine oocytes, for in vitro embryo production. |
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2022-11-01T12:46:51Z2022-11-01T12:46:51Z2001-06-22http://repositorio.ufsm.br/handle/1/26723To evaluate the effect of maintenance of bovine oocytes in follicular fluid (LF) for in vitro production of embryos, 1322 Complex Cumulus Oophorus (CCO's) obtained from abattoir bovine ovaries were distributed in two experiments. In the experiment I, maturation performed in TCM-199 for 24 hours (Control), was compared with the maintenance in follicular fluid at 30ºC for two (LF2), four (LF4) and six hours (LF6), followed by complementary TCM-199 maturation for 22, 20 and 18 hours, respectively. In the experiment II, oocyte maturation in TCM-199 for 24 hours (Control) and 18 hours (M18), was compared with the maintenance in LF for 6 hours followed by 18 hours TCM-199 maturation (LF6+M18). In both experiments, after maturation, the oocytes were transferred to Fert-Talp medium added of heparin and fertilization performed with semen selected by swim-up procedure. After that, zygotes were denuded and cultured in SOFaa medium. In the experiment I, the cleavage rate observed was 81.6% in Control group, 80.0% in LF2 group, 80.7% in LF4 group, and 81.7% in LF6 group. The blastocyst rate in D9 was 22.3%, 19.4%, 24.4% and 26.7% for Control group, LF2, LF4 and LF6 groups, respectively. No differences were observed (p>0.05). In experiment II, the cleavage rates (79.8%, 76.2% and 84.9%) and day 9 blastocyst rates (26.4%, 27.7% and 35.4%) in Control group, M18 and LF6+M18 groups, respectively, were not significantly different (p>0.05). The results obtained suggest that it is possible to maintain bovine oocytes in fluid obtained from follicles with 2 to 8mm of diameter, for periods until 6 hours at 30ºC, resulting in a simple, practical, and efficient alternative for maintenance and transport of bovine oocytes, for in vitro embryo production.Com o objetivo de avaliar o efeito da manutenção de oócitos bovinos em líquido folicular (LF) para a produção in vitro de embriões, 1322 Complexos Cumulus oophorus (CCO’s) obtidos de ovários de abatedouro foram distribuídos em dois experimentos. No experimento I, comparou-se a maturação de oócitos em TCM-199 por 24 horas a 39C (Controle), com a manutenção em líquido folicular a 30oC por, respectivamente, duas (LF2), quatro (LF4) e seis horas (LF6), seguida da maturação complementar por 22, 20 e 18 horas em TCM-199. No experimento II, avaliou-se o efeito da maturação de oócitos em TCM-199 por 24 (Controle) e 18horas (M18). Comparou-se esses resultados com a manutenção de oócitos em LF por 6 horas seguida da maturação por 18 horas em TCM-199 (LF6+M18). Nos dois experimentos, após a maturação, os oócitos foram transferidos para o meio Fert-Talp adicionado de heparina e realizada a fecundação com sêmen selecionado pelo processo de migração ascendente. A seguir procedeu-se o desnudamento e o cultivo dos zigotos em meio SOFaa. No experimento I não se verificou diferença na clivagem entre o Grupo controle (81,6%), LF2 (80,0%), LF4 (80,7%) e LF6 (81,7%). A taxa de blastocistos em D9 foi de 22,3%, 19,4%, 24,4% e 26,7% para os grupos Controle, LF2, LF4 e LF6, respectivamente. No experimento II obteve-se uma taxa de clivagem de 79,8%, 76,2%, e 84,9% e uma taxa de blastocistos em D9 de 26,4%, 27,7% e 35,4% para os grupos Controle, M18 e LF6+M18, respectivamente. Não foram observadas diferenças significativas entre as taxas de clivagem e de blastocistos em ambos os experimentos. Os resultados obtidos sugerem que é possível manter oócitos bovinos em líquido folicular obtido de folículos com 2 a 8mm de diâmetro, por períodos de até 6 horas a 30oC, gerando uma alternativa prática, eficiente e simples para a manutenção dos oócitos para a produção in vitro de embriões.porUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em Medicina VeterináriaUFSMBrasilMedicina VeterináriaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessLíquido folicularMaturaçãoTransporteOócitosBovinosFollicular fluidMaturationTransportOocytesBovineCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIADesenvolvimento de oócitos bovinos mantidos em líquido folicularDevelopment of bovine oocytes maintained in follicular fluidinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSilva, Carlos Antonio Mondinohttp://lattes.cnpq.br/8811861305924545Bernardi, Mari LourdesPegoraro, Ligia Margareth Cantarellihttp://lattes.cnpq.br/8062191602972072Lehmkuhl, Ricardo Coelho500500000007600600600600600903ff6ef-b847-4354-89fa-8bcae7add9e08110e55c-ba17-4239-8e30-c41ad66a3dc1497251df-40c2-4223-85cc-4e42265b41ee6c537ea4-8297-40b4-97a3-1d6c762d4785reponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGMV_2001_LEHMKUHL_RICARDO.pdfDIS_PPGMV_2001_LEHMKUHL_RICARDO.pdfDissertação de mestradoapplication/pdf610174http://repositorio.ufsm.br/bitstream/1/26723/1/DIS_PPGMV_2001_LEHMKUHL_RICARDO.pdf463992a75bf1f7065e7a69aa5c09721bMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/26723/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/26723/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD531/267232022-11-01 09:46:52.074oai:repositorio.ufsm.br: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ório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-11-01T12:46:52Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Desenvolvimento de oócitos bovinos mantidos em líquido folicular |
dc.title.alternative.eng.fl_str_mv |
Development of bovine oocytes maintained in follicular fluid |
title |
Desenvolvimento de oócitos bovinos mantidos em líquido folicular |
spellingShingle |
Desenvolvimento de oócitos bovinos mantidos em líquido folicular Lehmkuhl, Ricardo Coelho Líquido folicular Maturação Transporte Oócitos Bovinos Follicular fluid Maturation Transport Oocytes Bovine CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Desenvolvimento de oócitos bovinos mantidos em líquido folicular |
title_full |
Desenvolvimento de oócitos bovinos mantidos em líquido folicular |
title_fullStr |
Desenvolvimento de oócitos bovinos mantidos em líquido folicular |
title_full_unstemmed |
Desenvolvimento de oócitos bovinos mantidos em líquido folicular |
title_sort |
Desenvolvimento de oócitos bovinos mantidos em líquido folicular |
author |
Lehmkuhl, Ricardo Coelho |
author_facet |
Lehmkuhl, Ricardo Coelho |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Carlos Antonio Mondino |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8811861305924545 |
dc.contributor.referee1.fl_str_mv |
Bernardi, Mari Lourdes |
dc.contributor.referee2.fl_str_mv |
Pegoraro, Ligia Margareth Cantarelli |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8062191602972072 |
dc.contributor.author.fl_str_mv |
Lehmkuhl, Ricardo Coelho |
contributor_str_mv |
Silva, Carlos Antonio Mondino Bernardi, Mari Lourdes Pegoraro, Ligia Margareth Cantarelli |
dc.subject.por.fl_str_mv |
Líquido folicular Maturação Transporte Oócitos Bovinos |
topic |
Líquido folicular Maturação Transporte Oócitos Bovinos Follicular fluid Maturation Transport Oocytes Bovine CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Follicular fluid Maturation Transport Oocytes Bovine |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
To evaluate the effect of maintenance of bovine oocytes in follicular fluid (LF) for in vitro production of embryos, 1322 Complex Cumulus Oophorus (CCO's) obtained from abattoir bovine ovaries were distributed in two experiments. In the experiment I, maturation performed in TCM-199 for 24 hours (Control), was compared with the maintenance in follicular fluid at 30ºC for two (LF2), four (LF4) and six hours (LF6), followed by complementary TCM-199 maturation for 22, 20 and 18 hours, respectively. In the experiment II, oocyte maturation in TCM-199 for 24 hours (Control) and 18 hours (M18), was compared with the maintenance in LF for 6 hours followed by 18 hours TCM-199 maturation (LF6+M18). In both experiments, after maturation, the oocytes were transferred to Fert-Talp medium added of heparin and fertilization performed with semen selected by swim-up procedure. After that, zygotes were denuded and cultured in SOFaa medium. In the experiment I, the cleavage rate observed was 81.6% in Control group, 80.0% in LF2 group, 80.7% in LF4 group, and 81.7% in LF6 group. The blastocyst rate in D9 was 22.3%, 19.4%, 24.4% and 26.7% for Control group, LF2, LF4 and LF6 groups, respectively. No differences were observed (p>0.05). In experiment II, the cleavage rates (79.8%, 76.2% and 84.9%) and day 9 blastocyst rates (26.4%, 27.7% and 35.4%) in Control group, M18 and LF6+M18 groups, respectively, were not significantly different (p>0.05). The results obtained suggest that it is possible to maintain bovine oocytes in fluid obtained from follicles with 2 to 8mm of diameter, for periods until 6 hours at 30ºC, resulting in a simple, practical, and efficient alternative for maintenance and transport of bovine oocytes, for in vitro embryo production. |
publishDate |
2001 |
dc.date.issued.fl_str_mv |
2001-06-22 |
dc.date.accessioned.fl_str_mv |
2022-11-01T12:46:51Z |
dc.date.available.fl_str_mv |
2022-11-01T12:46:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/26723 |
url |
http://repositorio.ufsm.br/handle/1/26723 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
500500000007 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Medicina Veterinária |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
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