Fitoquímica e atividades antirradicalar, fotoprotetora e antibacteriana de Ruellia angustiflora (Nees) Lindau ex Rambo
Ano de defesa: | 2023 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências da Saúde |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Farmacêuticas
|
Departamento: |
Farmácia
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/30191 |
Resumo: | The use of plants for their medicinal properties is as old as mankind itself. Ruellia angustiflora (Acanthaceae) is a species popularly known as “fire flower” and used in traditional medicine as a wound healing agent. However, few studies are found in the literature about its chemical composition and biological activities. Thus, this work aimed to carry out a phytochemical study and determine the antiradical, photoprotective and antibacterial activities of the root (Ra-R), stem (Ra-C) and leaves (Ra-F) of this species. The extractions (0.2%; 1% and 5%) were carried out in an ultrasound bath and 95% ethanol as solvent, and the process was monitored to determine the ideal time, for 5 hours. The content of total phenolic compounds, flavonoids and tannins was determined, and the characterization of the extracts was performed by HPLC-UV. The antiradical activity was determined by the DPPH method, the in vitro photoprotective activity was evaluated by spectrophotometry and the antibacterial activity was performed by the agar perforation method. Extraction rates were more pronounced in the first minutes, but after 60 minutes they remained practically stable. Thus, 60 minutes was considered the ideal extraction time, aiming at optimizing the process. The solid/liquid ratio was also an analyzed parameter, and according to the extraction efficiency, yield and cost-effectiveness, 1% extracts (m/v) were prioritized for additional tests. In the phytochemical analysis, Ra-R showed the highest amount of phenolic compounds, and Ra-F showed the highest amount of flavonoids. Condensed tannins were not identified in the extracts. The kinetics of the DPPH reaction with the extracts showed a biphasic behavior, with a rapid decay of the radical concentration in the first minutes, followed by a slow step. Ra-R and Ra-F showed similar IC50 values, while Ra-C showed higher values, which is probably due to its lower content of total phenolic compounds. Five phenolic compounds were identified in the extracts: caffeic acid, ferulic acid (main peak), rutin, quercetin and luteolin. Through the DPPH-HPLC screening, it was observed that ferulic acid, rutin and quercetin are mainly responsible for the antiradical activity of the extracts. The extracts showed significant absorption in the UV region of the spectrum and relevant photoprotective activity, especially Ra-F (SPF = 23), possibly due to its high content of flavonoids. The antibacterial activity of the extracts was also verified against Gram-positive bacteria Staphylococcus aureus and Streptococcus pyogenes, species frequently associated with skin infections, and Ra-R showed a halo of inhibition similar to erythromycin on S. pyogenes. The extracts were inactive against Gram-negative bacteria by the method used. Together, these data reveal the potential of Ruellia angustiflora as an active ingredient for the development of multifunctional formulations. |
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2023-09-11T15:01:26Z2023-09-11T15:01:26Z2023-09-01http://repositorio.ufsm.br/handle/1/30191The use of plants for their medicinal properties is as old as mankind itself. Ruellia angustiflora (Acanthaceae) is a species popularly known as “fire flower” and used in traditional medicine as a wound healing agent. However, few studies are found in the literature about its chemical composition and biological activities. Thus, this work aimed to carry out a phytochemical study and determine the antiradical, photoprotective and antibacterial activities of the root (Ra-R), stem (Ra-C) and leaves (Ra-F) of this species. The extractions (0.2%; 1% and 5%) were carried out in an ultrasound bath and 95% ethanol as solvent, and the process was monitored to determine the ideal time, for 5 hours. The content of total phenolic compounds, flavonoids and tannins was determined, and the characterization of the extracts was performed by HPLC-UV. The antiradical activity was determined by the DPPH method, the in vitro photoprotective activity was evaluated by spectrophotometry and the antibacterial activity was performed by the agar perforation method. Extraction rates were more pronounced in the first minutes, but after 60 minutes they remained practically stable. Thus, 60 minutes was considered the ideal extraction time, aiming at optimizing the process. The solid/liquid ratio was also an analyzed parameter, and according to the extraction efficiency, yield and cost-effectiveness, 1% extracts (m/v) were prioritized for additional tests. In the phytochemical analysis, Ra-R showed the highest amount of phenolic compounds, and Ra-F showed the highest amount of flavonoids. Condensed tannins were not identified in the extracts. The kinetics of the DPPH reaction with the extracts showed a biphasic behavior, with a rapid decay of the radical concentration in the first minutes, followed by a slow step. Ra-R and Ra-F showed similar IC50 values, while Ra-C showed higher values, which is probably due to its lower content of total phenolic compounds. Five phenolic compounds were identified in the extracts: caffeic acid, ferulic acid (main peak), rutin, quercetin and luteolin. Through the DPPH-HPLC screening, it was observed that ferulic acid, rutin and quercetin are mainly responsible for the antiradical activity of the extracts. The extracts showed significant absorption in the UV region of the spectrum and relevant photoprotective activity, especially Ra-F (SPF = 23), possibly due to its high content of flavonoids. The antibacterial activity of the extracts was also verified against Gram-positive bacteria Staphylococcus aureus and Streptococcus pyogenes, species frequently associated with skin infections, and Ra-R showed a halo of inhibition similar to erythromycin on S. pyogenes. The extracts were inactive against Gram-negative bacteria by the method used. Together, these data reveal the potential of Ruellia angustiflora as an active ingredient for the development of multifunctional formulations.O uso de plantas por suas propriedades medicinais é tão antigo quanto a própria humanidade. Ruellia angustiflora (Acanthaceae) é uma espécie conhecida popularmente como “flor-defogo” e utilizada na medicina tradicional como cicatrizante de feridas. No entanto, poucos estudos são encontrados na literatura acerca de sua composição química e atividades biológicas. Assim, este trabalho teve como objetivo realizar um estudo fitoquímico e determinar as atividades antirradicalar, fotoprotetora e antibacteriana da raiz (Ra-R), caule (Ra-C) e folhas (Ra-F) desta espécie. As extrações (0,2%; 1% e 5%) foram realizadas em banho de ultrassom e etanol 95% como solvente, e o processo foi acompanhado para a determinação do tempo ideal, por 5 horas. Foi determinado o teor de compostos fenólicos totais, flavonoides e taninos, e a caracterização dos extratos foi realizada por CLAE-UV. A atividade antirradicalar foi determinada pelo método do DPPH, a atividade fotoprotetora in vitro foi avaliada por espectrofotometria e a atividade antibacteriana foi realizada pelo método de perfuração em ágar. As taxas de extração foram mais acentuadas nos primeiros minutos, mas após 60 minutos permaneceram praticamente estáveis. Assim, 60 minutos foi considerado o tempo ideal de extração, visando a otimização do processo. A razão sólido/líquido também foi um parâmetro analisado, e de acordo com a eficiência de extração, rendimento e custo-benefício, priorizaramse os extratos a 1% (m/v) para a realização dos testes adicionais. Na análise fitoquímica, Ra-R demonstrou a maior quantidade de compostos fenólicos, e Ra-F apresentou a maior quantidade de flavonoides. Não foram identificados taninos condensados nos extratos. A cinética da reação do DPPH com os extratos apresentou comportamento bifásico, com um rápido decaimento da concentração do radical nos primeiros minutos, seguidos por uma etapa lenta. Ra-R e Ra-F apresentaram valores de IC50 semelhantes, enquanto Ra-C mostrou valores maiores, o que se deve, provavelmente, ao seu menor teor de compostos fenólicos totais. Foram identificados cinco compostos fenólicos nos extratos: ácido cafeico, ácido ferúlico (pico principal), rutina, quercetina e luteolina. Por meio do screening DPPH-CLAE, foi observado que o ácido ferúlico, a rutina e a quercetina são os principais responsáveis pela atividade antiradicalar dos extratos. Os extratos apresentaram significativa absorção na região UV do espectro e atividade fotoprotetora relevante, com destaque para Ra-F (FPS = 23), possivelmente devido ao seu alto teor de flavonoides. Também foi verificada a atividade antibacteriana dos extratos frente a bactérias Gram-positivas Staphylococcus aureus e Streptococcus pyogenes, espécies frequentemente associadas a infecções cutâneas, e Ra-R mostrou halo de inibição similar à eritromicina sobre S. pyogenes. Os extratos foram inativos frente a bactérias Gram-negativas pelo método utilizado. Em conjunto, estes dados revelam o potencial de Ruellia angustiflora como ativo para o desenvolvimento de formulações multifuncionais.porUniversidade Federal de Santa MariaCentro de Ciências da SaúdePrograma de Pós-Graduação em Ciências FarmacêuticasUFSMBrasilFarmáciaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessRuellia angustifloraCompostos fenólicosAtividade antirradicalarFotoprotetores naturaisAtividade antibacterianaPhenolic compoundsAntiradical activityNatural sunscreensAntibacterial activityCNPQ::CIENCIAS DA SAUDE::FARMACIAFitoquímica e atividades antirradicalar, fotoprotetora e antibacteriana de Ruellia angustiflora (Nees) Lindau ex RamboPhytochemical and anti-radical, photoprotective and antibacterial activities of Ruellia angustiflora (Nees) Lindau ex Ramboinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisRosa, Marcelo Barcellos dahttp://lattes.cnpq.br/0308293154958870Carvalho, Camilo Amaro deCardoso, Carmem DickowSilva, Elisabete de Avila daBauermann, Liliane de Freitashttp://lattes.cnpq.br/5637430699482543Zadra, Marina4003000000056006006006006006006008b5ece49-3859-41c1-a27a-94fe03364b5a2dfbad32-dd0e-40f0-873c-23e382680098a0b9f40f-6e86-4184-a359-adda3afd5d83f89d9ee8-47bb-4fd7-af41-8939f2ee36cf3dbf1a62-6139-4e85-8a97-c5af5d229ccb2692a8bc-fe74-4278-b83e-83c3d8a4024dreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/30191/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52ORIGINALTES_PPGCF_2023_ZADRA_MARINA.pdfTES_PPGCF_2023_ZADRA_MARINA.pdfTese de doutoradoapplication/pdf2283463http://repositorio.ufsm.br/bitstream/1/30191/1/TES_PPGCF_2023_ZADRA_MARINA.pdf237c6fe52264bdc980de473383e92b96MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/30191/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD531/301912023-09-11 12:01:26.645oai:repositorio.ufsm.br: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ório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132023-09-11T15:01:26Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Fitoquímica e atividades antirradicalar, fotoprotetora e antibacteriana de Ruellia angustiflora (Nees) Lindau ex Rambo |
dc.title.alternative.eng.fl_str_mv |
Phytochemical and anti-radical, photoprotective and antibacterial activities of Ruellia angustiflora (Nees) Lindau ex Rambo |
title |
Fitoquímica e atividades antirradicalar, fotoprotetora e antibacteriana de Ruellia angustiflora (Nees) Lindau ex Rambo |
spellingShingle |
Fitoquímica e atividades antirradicalar, fotoprotetora e antibacteriana de Ruellia angustiflora (Nees) Lindau ex Rambo Zadra, Marina Ruellia angustiflora Compostos fenólicos Atividade antirradicalar Fotoprotetores naturais Atividade antibacteriana Phenolic compounds Antiradical activity Natural sunscreens Antibacterial activity CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Fitoquímica e atividades antirradicalar, fotoprotetora e antibacteriana de Ruellia angustiflora (Nees) Lindau ex Rambo |
title_full |
Fitoquímica e atividades antirradicalar, fotoprotetora e antibacteriana de Ruellia angustiflora (Nees) Lindau ex Rambo |
title_fullStr |
Fitoquímica e atividades antirradicalar, fotoprotetora e antibacteriana de Ruellia angustiflora (Nees) Lindau ex Rambo |
title_full_unstemmed |
Fitoquímica e atividades antirradicalar, fotoprotetora e antibacteriana de Ruellia angustiflora (Nees) Lindau ex Rambo |
title_sort |
Fitoquímica e atividades antirradicalar, fotoprotetora e antibacteriana de Ruellia angustiflora (Nees) Lindau ex Rambo |
author |
Zadra, Marina |
author_facet |
Zadra, Marina |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Rosa, Marcelo Barcellos da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0308293154958870 |
dc.contributor.referee1.fl_str_mv |
Carvalho, Camilo Amaro de |
dc.contributor.referee2.fl_str_mv |
Cardoso, Carmem Dickow |
dc.contributor.referee3.fl_str_mv |
Silva, Elisabete de Avila da |
dc.contributor.referee4.fl_str_mv |
Bauermann, Liliane de Freitas |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5637430699482543 |
dc.contributor.author.fl_str_mv |
Zadra, Marina |
contributor_str_mv |
Rosa, Marcelo Barcellos da Carvalho, Camilo Amaro de Cardoso, Carmem Dickow Silva, Elisabete de Avila da Bauermann, Liliane de Freitas |
dc.subject.por.fl_str_mv |
Ruellia angustiflora Compostos fenólicos Atividade antirradicalar Fotoprotetores naturais Atividade antibacteriana |
topic |
Ruellia angustiflora Compostos fenólicos Atividade antirradicalar Fotoprotetores naturais Atividade antibacteriana Phenolic compounds Antiradical activity Natural sunscreens Antibacterial activity CNPQ::CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Phenolic compounds Antiradical activity Natural sunscreens Antibacterial activity |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
The use of plants for their medicinal properties is as old as mankind itself. Ruellia angustiflora (Acanthaceae) is a species popularly known as “fire flower” and used in traditional medicine as a wound healing agent. However, few studies are found in the literature about its chemical composition and biological activities. Thus, this work aimed to carry out a phytochemical study and determine the antiradical, photoprotective and antibacterial activities of the root (Ra-R), stem (Ra-C) and leaves (Ra-F) of this species. The extractions (0.2%; 1% and 5%) were carried out in an ultrasound bath and 95% ethanol as solvent, and the process was monitored to determine the ideal time, for 5 hours. The content of total phenolic compounds, flavonoids and tannins was determined, and the characterization of the extracts was performed by HPLC-UV. The antiradical activity was determined by the DPPH method, the in vitro photoprotective activity was evaluated by spectrophotometry and the antibacterial activity was performed by the agar perforation method. Extraction rates were more pronounced in the first minutes, but after 60 minutes they remained practically stable. Thus, 60 minutes was considered the ideal extraction time, aiming at optimizing the process. The solid/liquid ratio was also an analyzed parameter, and according to the extraction efficiency, yield and cost-effectiveness, 1% extracts (m/v) were prioritized for additional tests. In the phytochemical analysis, Ra-R showed the highest amount of phenolic compounds, and Ra-F showed the highest amount of flavonoids. Condensed tannins were not identified in the extracts. The kinetics of the DPPH reaction with the extracts showed a biphasic behavior, with a rapid decay of the radical concentration in the first minutes, followed by a slow step. Ra-R and Ra-F showed similar IC50 values, while Ra-C showed higher values, which is probably due to its lower content of total phenolic compounds. Five phenolic compounds were identified in the extracts: caffeic acid, ferulic acid (main peak), rutin, quercetin and luteolin. Through the DPPH-HPLC screening, it was observed that ferulic acid, rutin and quercetin are mainly responsible for the antiradical activity of the extracts. The extracts showed significant absorption in the UV region of the spectrum and relevant photoprotective activity, especially Ra-F (SPF = 23), possibly due to its high content of flavonoids. The antibacterial activity of the extracts was also verified against Gram-positive bacteria Staphylococcus aureus and Streptococcus pyogenes, species frequently associated with skin infections, and Ra-R showed a halo of inhibition similar to erythromycin on S. pyogenes. The extracts were inactive against Gram-negative bacteria by the method used. Together, these data reveal the potential of Ruellia angustiflora as an active ingredient for the development of multifunctional formulations. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-09-11T15:01:26Z |
dc.date.available.fl_str_mv |
2023-09-11T15:01:26Z |
dc.date.issued.fl_str_mv |
2023-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/30191 |
url |
http://repositorio.ufsm.br/handle/1/30191 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
400300000005 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 600 600 |
dc.relation.authority.fl_str_mv |
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dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Farmacêuticas |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Farmácia |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/30191/2/license_rdf http://repositorio.ufsm.br/bitstream/1/30191/1/TES_PPGCF_2023_ZADRA_MARINA.pdf http://repositorio.ufsm.br/bitstream/1/30191/3/license.txt |
bitstream.checksum.fl_str_mv |
4460e5956bc1d1639be9ae6146a50347 237c6fe52264bdc980de473383e92b96 2f0571ecee68693bd5cd3f17c1e075df |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
|
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1794524284549332992 |