Desenvolvimento de método multirresíduo para determinação de agrotóxicos em suco de uva integral utilizando método QuEChERS acetato e LC-MS/MS
Ano de defesa: | 2021 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Química
|
Departamento: |
Química
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/23077 |
Resumo: | In the last century, pesticides have been widely used in agriculture due to the many benefits related to the quality and quantity of crops produced. Although they contribute to pest control and increased productivity, the indiscriminate use of pesticides is harmful to human health and the environment. Thus, it became necessary to determine a maximum amount of product that can be present in food, called Maximum Limits and Residues - MRLs. Viticulture is extremely important in Brazilian agriculture as it produces about 1.5 million tons of grapes per year and a large part of the cultivation takes place in the southern region of the country. Half of the grapes produced are sent to processing where by-products are obtained, such as wine and grape juice. Grape juice, because it is easy to prepare and has a high nutritional value, is a drink widely consumed by Brazilians. The quality of grape juice depends directly on the quality of the fruits used in its production, which depends on the use of pesticides. The use of multiresidue methods that are capable of determining different classes and compounds in a single analytical procedure is the best way to carry out the monitoring of pesticide residues in different matrices. Based on this, this work aimed to determine pesticide residues in grape juice through the optimization of the QuEChERS method and analysis by liquid chromatography with tandem mass spectrometry (LC-MS/MS). The parameters that influence the extraction efficiency were studied and, after finding the best extraction conditions, the method was validated. The analysis by LC-MS/MS allowed the determination of the analytes with high selectivity and detectability. The QuEChERS modified acetate method was validated for 153 compounds and the limit of quantification of the method (LOQ) varied from 5 to 10 μg L-1. The validated method was used for the determination of pesticides in grape juice samples. In 17 samples, pesticide residues of different classes and characteristics were found in concentrations ranging from concentrations lower than the LOQ to 41.98 μg L-1. Considering the results obtained, the use of the modified QuEChERS acetate method together with the use of LC-MS/MS proved to be efficient in multi-residue determination of pesticides in grape juice. |
id |
UFSM_1e89e076990c439eb0595534c904d42a |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/23077 |
network_acronym_str |
UFSM |
network_name_str |
Biblioteca Digital de Teses e Dissertações do UFSM |
repository_id_str |
|
spelling |
2021-11-30T17:05:07Z2021-11-30T17:05:07Z2021-02-26http://repositorio.ufsm.br/handle/1/23077In the last century, pesticides have been widely used in agriculture due to the many benefits related to the quality and quantity of crops produced. Although they contribute to pest control and increased productivity, the indiscriminate use of pesticides is harmful to human health and the environment. Thus, it became necessary to determine a maximum amount of product that can be present in food, called Maximum Limits and Residues - MRLs. Viticulture is extremely important in Brazilian agriculture as it produces about 1.5 million tons of grapes per year and a large part of the cultivation takes place in the southern region of the country. Half of the grapes produced are sent to processing where by-products are obtained, such as wine and grape juice. Grape juice, because it is easy to prepare and has a high nutritional value, is a drink widely consumed by Brazilians. The quality of grape juice depends directly on the quality of the fruits used in its production, which depends on the use of pesticides. The use of multiresidue methods that are capable of determining different classes and compounds in a single analytical procedure is the best way to carry out the monitoring of pesticide residues in different matrices. Based on this, this work aimed to determine pesticide residues in grape juice through the optimization of the QuEChERS method and analysis by liquid chromatography with tandem mass spectrometry (LC-MS/MS). The parameters that influence the extraction efficiency were studied and, after finding the best extraction conditions, the method was validated. The analysis by LC-MS/MS allowed the determination of the analytes with high selectivity and detectability. The QuEChERS modified acetate method was validated for 153 compounds and the limit of quantification of the method (LOQ) varied from 5 to 10 μg L-1. The validated method was used for the determination of pesticides in grape juice samples. In 17 samples, pesticide residues of different classes and characteristics were found in concentrations ranging from concentrations lower than the LOQ to 41.98 μg L-1. Considering the results obtained, the use of the modified QuEChERS acetate method together with the use of LC-MS/MS proved to be efficient in multi-residue determination of pesticides in grape juice.No último século, os agrotóxicos tiveram seu uso disseminado na agricultura devido aos muitos benefícios relacionados a qualidade e quantidade de culturas produzidas. Apesar de contribuírem no controle de pragas e no aumento da produtividade, o uso indiscriminado de agrotóxicos traz prejuízos a saúde humana e ao meio ambiente. Dessa forma, tornou-se necessário a determinação de uma quantidade máxima de produto que pode estar presente nos alimentos, chamada de Limites Máximos de Resíduos – LMRs. A viticultura é extremamente importante na agricultura brasileira pois produz cerca de 1,5 milhões de toneladas de uva por ano e, grande parte do cultivo ocorre na região sul do país. Metade das uvas produzidas são encaminhadas para o processamento onde são obtidos subprodutos como o vinho e o suco de uva. O suco de uva por ser de fácil elaboração e possuir um alto valor nutricional é uma bebida muito consumida pelos brasileiros. A qualidade do suco de uva depende diretamente da qualidade das frutas utilizadas em sua produção o que é dependente do uso de agrotóxicos. O emprego de métodos multirresíduos que sejam capazes de determinar diferentes classes e compostos em um único procedimento analítico é a melhor maneira de realizar o monitoramento de resíduos de agrotóxicos em diferentes matrizes. Com base nisso, esse trabalho teve como objetivo a determinação de resíduos de agrotóxicos em suco de uva através da otimização do método QuEChERS e análise por cromatografia líquida acoplada à espectrometria de massas em série (LC-MS/MS). Os parâmetros que influenciam na eficiência de extração foram estudados e, após encontrar-se as melhores condições de extração, o método foi validado. A análise por LC-MS/MS permitiu a determinação dos analitos com alta seletividade e detectabilidade. O método QuEChERS acetato modificado foi validado para 153 compostos sendo que o limite de quantificação do método (LOQ) variou de 5 a 10 μg L-1. O método validado foi utilizado para a determinação de agrotóxicos em amostras de suco de uva. Foram encontrados, em 17 amostras, resíduos de agrotóxicos de diferentes classes e características em concentrações que variam de concentrações menores que o LOQ até 41,98 μg L-1. Considerando os resultados obtidos, a utilização do método QuEChERS acetato modificado juntamente com o uso de LC-MS/MS demonstrou ser eficiente na determinação multirresíduo de agrotóxicos em suco de uva.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em QuímicaUFSMBrasilQuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessQuEChERSAgrotóxicosLC-MS/MSSuco de uvaPesticidesGrape juiceCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICADesenvolvimento de método multirresíduo para determinação de agrotóxicos em suco de uva integral utilizando método QuEChERS acetato e LC-MS/MSDevelopment of a pesticide multi-residue determination method in of in raw grape juices using quechers acetate and LC-MS/MS.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPrestes, Osmar Damianhttp://lattes.cnpq.br/9632234955509028Müller, Aline Lima HermesMaldaner, Lianehttp://lattes.cnpq.br/7196053261911696Albarnaz, Karolaine da Silva100600000000600600600f79cc0c9-c923-4244-9c1f-c80d7467441089b5692f-cf24-4145-a815-7f4e71b00eff3a5b86e4-a4fc-4124-b40b-b860a9eed50b5d5ff8f2-6993-4986-bbe9-895e14d5fbedreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGQUIMICA_2021_ALBARNAZ_KAROLAINE.pdfDIS_PPGQUIMICA_2021_ALBARNAZ_KAROLAINE.pdfDissertação de Mestradoapplication/pdf3025497http://repositorio.ufsm.br/bitstream/1/23077/1/DIS_PPGQUIMICA_2021_ALBARNAZ_KAROLAINE.pdf2b3903c22b56afaf0a263f2f72469e72MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/23077/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/23077/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52TEXTDIS_PPGQUIMICA_2021_ALBARNAZ_KAROLAINE.pdf.txtDIS_PPGQUIMICA_2021_ALBARNAZ_KAROLAINE.pdf.txtExtracted texttext/plain215723http://repositorio.ufsm.br/bitstream/1/23077/4/DIS_PPGQUIMICA_2021_ALBARNAZ_KAROLAINE.pdf.txtc3530491d2581e50c457466038556f64MD54THUMBNAILDIS_PPGQUIMICA_2021_ALBARNAZ_KAROLAINE.pdf.jpgDIS_PPGQUIMICA_2021_ALBARNAZ_KAROLAINE.pdf.jpgIM Thumbnailimage/jpeg2830http://repositorio.ufsm.br/bitstream/1/23077/5/DIS_PPGQUIMICA_2021_ALBARNAZ_KAROLAINE.pdf.jpg72408d6e3aaf75fd56164db977603d7aMD551/230772023-05-10 11:49:37.733oai:repositorio.ufsm.br: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 Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-05-10T14:49:37Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Desenvolvimento de método multirresíduo para determinação de agrotóxicos em suco de uva integral utilizando método QuEChERS acetato e LC-MS/MS |
dc.title.alternative.eng.fl_str_mv |
Development of a pesticide multi-residue determination method in of in raw grape juices using quechers acetate and LC-MS/MS. |
title |
Desenvolvimento de método multirresíduo para determinação de agrotóxicos em suco de uva integral utilizando método QuEChERS acetato e LC-MS/MS |
spellingShingle |
Desenvolvimento de método multirresíduo para determinação de agrotóxicos em suco de uva integral utilizando método QuEChERS acetato e LC-MS/MS Albarnaz, Karolaine da Silva QuEChERS Agrotóxicos LC-MS/MS Suco de uva Pesticides Grape juice CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Desenvolvimento de método multirresíduo para determinação de agrotóxicos em suco de uva integral utilizando método QuEChERS acetato e LC-MS/MS |
title_full |
Desenvolvimento de método multirresíduo para determinação de agrotóxicos em suco de uva integral utilizando método QuEChERS acetato e LC-MS/MS |
title_fullStr |
Desenvolvimento de método multirresíduo para determinação de agrotóxicos em suco de uva integral utilizando método QuEChERS acetato e LC-MS/MS |
title_full_unstemmed |
Desenvolvimento de método multirresíduo para determinação de agrotóxicos em suco de uva integral utilizando método QuEChERS acetato e LC-MS/MS |
title_sort |
Desenvolvimento de método multirresíduo para determinação de agrotóxicos em suco de uva integral utilizando método QuEChERS acetato e LC-MS/MS |
author |
Albarnaz, Karolaine da Silva |
author_facet |
Albarnaz, Karolaine da Silva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Prestes, Osmar Damian |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9632234955509028 |
dc.contributor.referee1.fl_str_mv |
Müller, Aline Lima Hermes |
dc.contributor.referee2.fl_str_mv |
Maldaner, Liane |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7196053261911696 |
dc.contributor.author.fl_str_mv |
Albarnaz, Karolaine da Silva |
contributor_str_mv |
Prestes, Osmar Damian Müller, Aline Lima Hermes Maldaner, Liane |
dc.subject.por.fl_str_mv |
QuEChERS Agrotóxicos LC-MS/MS Suco de uva |
topic |
QuEChERS Agrotóxicos LC-MS/MS Suco de uva Pesticides Grape juice CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Pesticides Grape juice |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
In the last century, pesticides have been widely used in agriculture due to the many benefits related to the quality and quantity of crops produced. Although they contribute to pest control and increased productivity, the indiscriminate use of pesticides is harmful to human health and the environment. Thus, it became necessary to determine a maximum amount of product that can be present in food, called Maximum Limits and Residues - MRLs. Viticulture is extremely important in Brazilian agriculture as it produces about 1.5 million tons of grapes per year and a large part of the cultivation takes place in the southern region of the country. Half of the grapes produced are sent to processing where by-products are obtained, such as wine and grape juice. Grape juice, because it is easy to prepare and has a high nutritional value, is a drink widely consumed by Brazilians. The quality of grape juice depends directly on the quality of the fruits used in its production, which depends on the use of pesticides. The use of multiresidue methods that are capable of determining different classes and compounds in a single analytical procedure is the best way to carry out the monitoring of pesticide residues in different matrices. Based on this, this work aimed to determine pesticide residues in grape juice through the optimization of the QuEChERS method and analysis by liquid chromatography with tandem mass spectrometry (LC-MS/MS). The parameters that influence the extraction efficiency were studied and, after finding the best extraction conditions, the method was validated. The analysis by LC-MS/MS allowed the determination of the analytes with high selectivity and detectability. The QuEChERS modified acetate method was validated for 153 compounds and the limit of quantification of the method (LOQ) varied from 5 to 10 μg L-1. The validated method was used for the determination of pesticides in grape juice samples. In 17 samples, pesticide residues of different classes and characteristics were found in concentrations ranging from concentrations lower than the LOQ to 41.98 μg L-1. Considering the results obtained, the use of the modified QuEChERS acetate method together with the use of LC-MS/MS proved to be efficient in multi-residue determination of pesticides in grape juice. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-11-30T17:05:07Z |
dc.date.available.fl_str_mv |
2021-11-30T17:05:07Z |
dc.date.issued.fl_str_mv |
2021-02-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/23077 |
url |
http://repositorio.ufsm.br/handle/1/23077 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
100600000000 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.authority.fl_str_mv |
f79cc0c9-c923-4244-9c1f-c80d74674410 89b5692f-cf24-4145-a815-7f4e71b00eff 3a5b86e4-a4fc-4124-b40b-b860a9eed50b 5d5ff8f2-6993-4986-bbe9-895e14d5fbed |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Química |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Química |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações do UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Biblioteca Digital de Teses e Dissertações do UFSM |
collection |
Biblioteca Digital de Teses e Dissertações do UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/23077/1/DIS_PPGQUIMICA_2021_ALBARNAZ_KAROLAINE.pdf http://repositorio.ufsm.br/bitstream/1/23077/3/license.txt http://repositorio.ufsm.br/bitstream/1/23077/2/license_rdf http://repositorio.ufsm.br/bitstream/1/23077/4/DIS_PPGQUIMICA_2021_ALBARNAZ_KAROLAINE.pdf.txt http://repositorio.ufsm.br/bitstream/1/23077/5/DIS_PPGQUIMICA_2021_ALBARNAZ_KAROLAINE.pdf.jpg |
bitstream.checksum.fl_str_mv |
2b3903c22b56afaf0a263f2f72469e72 2f0571ecee68693bd5cd3f17c1e075df 4460e5956bc1d1639be9ae6146a50347 c3530491d2581e50c457466038556f64 72408d6e3aaf75fd56164db977603d7a |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1793240165952520192 |