Efeitos do herbicida clomazone sobre parâmetros metabólicos e antioxidantes em jundiá (Rhamdia quelen)
Ano de defesa: | 2004 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
|
Departamento: |
Bioquímica
|
País: |
BR
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/11171 |
Resumo: | The objective of this study was to evaluate the effect of clomazone herbicide on metabolism of silver catfish (Rhamdia quelen). It was measured levels of lactate, protein and glucose (plasma), glycogen, protein and lactate (liver and muscle), acetylcholinesterase (AChE) activity (brain and muscle), catalase activity (liver), TBARS formation (liver and brain), hematocrit and hemoglobin values, aminotransferases (AST and ALT) activities (liver and plasma) and histological analysis of hepatic tissue. Fishes were exposed to 0.5 and 1.0 mg/L of clomazone for 12, 24, 48, 96 and 192 h. These concentrations are used in rice fields. Some parameters were also assayed after 96 h and 192 h in water without herbicide (recovery periods) after 96 and 192 h of exposure. Levels of muscle glycogen and liver lactate were reduced as well as hematocrit value after experimental period. Hemoglobin content did not changed significantly. Lactate levels diminished in the muscle tissue. Liver glycogen, glucose and lactate in the plasma were enhanced after exposure to herbicide. Protein levels were enhanced in the liver and white muscle, except at last periods of exposure, while plasma protein increased from 48 to 96 h of exposure to the herbicide. Specific AChE activity decreased in brain and muscle after treatments. Catalase activity was reduced in all periods of exposure. Fishes exposed to clomazone increased TBARS production in the liver, while in the brain the fishes showed different responses with exposure period increasing. Aminotransferases activities were elevated in the plasma, while in the liver, ALT increased in the early periods only. Liver AST activity was enhanced after 12 h of exposure, and after 48 h its activity reduced significantly. Hepatic histological analysis presented hepatocyte vacuolation after 192 h of exposure to herbicide concentration of 1.0 mg/L. However, in recovery period of 192 h, hepatocyte vacuolation was observed in both clomazone concentration tested. Most of the metabolic disorders did not persist after recovery period, except to liver AST and ALT activities. The present work shows that clomazone concentrations used in rice fields can cause changes in metabolic state of the fish, which are of moderate intensity and generally reversible. |
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2017-04-242017-04-242004-09-10BIN, Márcia Crestani. Effects of clomazone herbicide on metabolic and antioxidant parameters in silver catfish(Rhamdia quelen). 2004. 109 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2004.http://repositorio.ufsm.br/handle/1/11171The objective of this study was to evaluate the effect of clomazone herbicide on metabolism of silver catfish (Rhamdia quelen). It was measured levels of lactate, protein and glucose (plasma), glycogen, protein and lactate (liver and muscle), acetylcholinesterase (AChE) activity (brain and muscle), catalase activity (liver), TBARS formation (liver and brain), hematocrit and hemoglobin values, aminotransferases (AST and ALT) activities (liver and plasma) and histological analysis of hepatic tissue. Fishes were exposed to 0.5 and 1.0 mg/L of clomazone for 12, 24, 48, 96 and 192 h. These concentrations are used in rice fields. Some parameters were also assayed after 96 h and 192 h in water without herbicide (recovery periods) after 96 and 192 h of exposure. Levels of muscle glycogen and liver lactate were reduced as well as hematocrit value after experimental period. Hemoglobin content did not changed significantly. Lactate levels diminished in the muscle tissue. Liver glycogen, glucose and lactate in the plasma were enhanced after exposure to herbicide. Protein levels were enhanced in the liver and white muscle, except at last periods of exposure, while plasma protein increased from 48 to 96 h of exposure to the herbicide. Specific AChE activity decreased in brain and muscle after treatments. Catalase activity was reduced in all periods of exposure. Fishes exposed to clomazone increased TBARS production in the liver, while in the brain the fishes showed different responses with exposure period increasing. Aminotransferases activities were elevated in the plasma, while in the liver, ALT increased in the early periods only. Liver AST activity was enhanced after 12 h of exposure, and after 48 h its activity reduced significantly. Hepatic histological analysis presented hepatocyte vacuolation after 192 h of exposure to herbicide concentration of 1.0 mg/L. However, in recovery period of 192 h, hepatocyte vacuolation was observed in both clomazone concentration tested. Most of the metabolic disorders did not persist after recovery period, except to liver AST and ALT activities. The present work shows that clomazone concentrations used in rice fields can cause changes in metabolic state of the fish, which are of moderate intensity and generally reversible.O objetivo deste estudo foi avaliar o efeito do herbicida clomazone sobre o metabolismo do jundiá (Rhamdia quelen). Foram medidos os níveis de lactato, proteína e glicose (plasma), glicogênio, proteína e lactato (fígado e músculo), atividade da enzima AChE (cérebro e músculo), atividade da enzima catalase (fígado), formação de TBARS (fígado e cérebro), valores de hematócrito e hemoglobina, atividade das enzimas aminotransferases (AST e ALT) (fígado e plasma) e análise histológica do tecido hepático. Os peixes foram expostos a 0.5 e 1.0 mg/L de clomazone por 12, 24, 48, 96 e 192 horas. Estas concentrações são utilizadas nas lavouras de arroz. Alguns parâmetros foram também medidos após 96 e 192 horas em água sem o herbicida (períodos de recuperação) após 96 e 192 horas de exposição. Os níveis de glicogênio muscular e lactato hepático estavam reduzidos, assim como o valor do hematócrito, após o período experimental. O conteúdo de hemoglobina não se alterou significativamente. Os níveis de lactato diminuíram no tecido muscular. O glicogênio hepático, glicose e lactato no plasma estavam aumentados após exposição ao herbicida. Os níveis de proteína estavam aumentados no fígado e músculo, exceto nos últimos tempos de exposição, enquanto a proteína no plasma aumentou a partir de 48 até 96 horas de exposição ao herbicida. A atividade específica da AChE diminuiu no cérebro e músculo após os tratamentos. A atividade da catalase foi reduzida em todos os períodos de exposição. Os peixes expostos ao clomazone aumentaram a produção de TBARS no fígado, enquanto no cérebro os peixes apresentaram diferentes respostas com o aumento do tempo de exposição. A atividade das aminotransferases estava elevada no plasma, enquanto no fígado, a ALT aumentou somente nos períodos iniciais. A atividade da AST no fígado estava aumentada após 12 horas de exposição, e após 48 horas, sua atividade reduziu significativamente. A análise histológica demonstrou vacuolização nos hepatócitos após 192 horas de exposição ao herbicida na concentração de 1,0 mg/L. Entretanto, no período de recuperação de 192 horas, a vacuolização foi observada em ambas concentrações testadas do clomazone. A maioria das desordens metabólicas não persistiu após o período de recuperação, exceto as atividades da AST e ALT hepáticas. O presente trabalho mostra que as concentrações de clomazone utilizadas nas lavouras de arroz podem causar mudanças no estado metabólico do peixe, às quais são de moderada intensidade e geralmente reversíveis.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaBioquímicaBioquímica toxicológicaHerbicidasJundiáMetabolismoCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEfeitos do herbicida clomazone sobre parâmetros metabólicos e antioxidantes em jundiá (Rhamdia quelen)Effects of clomazone herbicide on metabolic and antioxidant parameters in silver catfish(Rhamdia quelen)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisLoro, Vania Luciahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796333D7Schetinger, Maria Rosa ChitolinaBarcellos, Leonardo José Gilhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763175J2http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705255Y5Bin, Márcia Crestani200800000002400500300500500b0918185-c6b7-49df-b8d6-d245d73535b8a11848e7-962d-403b-be78-837faa066e56cba4a833-b1c5-4608-8453-7a46f671faf1de1fbfb1-090e-4afb-99c2-1e8307678fbcinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALMARCIACRESTANI.pdfapplication/pdf1047145http://repositorio.ufsm.br/bitstream/1/11171/1/MARCIACRESTANI.pdfc88d99e705a29db0e773ea21c79c41c3MD51TEXTMARCIACRESTANI.pdf.txtMARCIACRESTANI.pdf.txtExtracted texttext/plain136608http://repositorio.ufsm.br/bitstream/1/11171/2/MARCIACRESTANI.pdf.txt5c2d3bc54a55c3d27332ace558348495MD52THUMBNAILMARCIACRESTANI.pdf.jpgMARCIACRESTANI.pdf.jpgIM Thumbnailimage/jpeg5354http://repositorio.ufsm.br/bitstream/1/11171/3/MARCIACRESTANI.pdf.jpg9a004badaa4ea6d391699cf8d727086bMD531/111712017-07-25 12:09:55.252oai:repositorio.ufsm.br:1/11171Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2017-07-25T15:09:55Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Efeitos do herbicida clomazone sobre parâmetros metabólicos e antioxidantes em jundiá (Rhamdia quelen) |
dc.title.alternative.eng.fl_str_mv |
Effects of clomazone herbicide on metabolic and antioxidant parameters in silver catfish(Rhamdia quelen) |
title |
Efeitos do herbicida clomazone sobre parâmetros metabólicos e antioxidantes em jundiá (Rhamdia quelen) |
spellingShingle |
Efeitos do herbicida clomazone sobre parâmetros metabólicos e antioxidantes em jundiá (Rhamdia quelen) Bin, Márcia Crestani Bioquímica Bioquímica toxicológica Herbicidas Jundiá Metabolismo CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Efeitos do herbicida clomazone sobre parâmetros metabólicos e antioxidantes em jundiá (Rhamdia quelen) |
title_full |
Efeitos do herbicida clomazone sobre parâmetros metabólicos e antioxidantes em jundiá (Rhamdia quelen) |
title_fullStr |
Efeitos do herbicida clomazone sobre parâmetros metabólicos e antioxidantes em jundiá (Rhamdia quelen) |
title_full_unstemmed |
Efeitos do herbicida clomazone sobre parâmetros metabólicos e antioxidantes em jundiá (Rhamdia quelen) |
title_sort |
Efeitos do herbicida clomazone sobre parâmetros metabólicos e antioxidantes em jundiá (Rhamdia quelen) |
author |
Bin, Márcia Crestani |
author_facet |
Bin, Márcia Crestani |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Loro, Vania Lucia |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796333D7 |
dc.contributor.referee1.fl_str_mv |
Schetinger, Maria Rosa Chitolina |
dc.contributor.referee2.fl_str_mv |
Barcellos, Leonardo José Gil |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763175J2 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705255Y5 |
dc.contributor.author.fl_str_mv |
Bin, Márcia Crestani |
contributor_str_mv |
Loro, Vania Lucia Schetinger, Maria Rosa Chitolina Barcellos, Leonardo José Gil |
dc.subject.por.fl_str_mv |
Bioquímica Bioquímica toxicológica Herbicidas Jundiá Metabolismo |
topic |
Bioquímica Bioquímica toxicológica Herbicidas Jundiá Metabolismo CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
The objective of this study was to evaluate the effect of clomazone herbicide on metabolism of silver catfish (Rhamdia quelen). It was measured levels of lactate, protein and glucose (plasma), glycogen, protein and lactate (liver and muscle), acetylcholinesterase (AChE) activity (brain and muscle), catalase activity (liver), TBARS formation (liver and brain), hematocrit and hemoglobin values, aminotransferases (AST and ALT) activities (liver and plasma) and histological analysis of hepatic tissue. Fishes were exposed to 0.5 and 1.0 mg/L of clomazone for 12, 24, 48, 96 and 192 h. These concentrations are used in rice fields. Some parameters were also assayed after 96 h and 192 h in water without herbicide (recovery periods) after 96 and 192 h of exposure. Levels of muscle glycogen and liver lactate were reduced as well as hematocrit value after experimental period. Hemoglobin content did not changed significantly. Lactate levels diminished in the muscle tissue. Liver glycogen, glucose and lactate in the plasma were enhanced after exposure to herbicide. Protein levels were enhanced in the liver and white muscle, except at last periods of exposure, while plasma protein increased from 48 to 96 h of exposure to the herbicide. Specific AChE activity decreased in brain and muscle after treatments. Catalase activity was reduced in all periods of exposure. Fishes exposed to clomazone increased TBARS production in the liver, while in the brain the fishes showed different responses with exposure period increasing. Aminotransferases activities were elevated in the plasma, while in the liver, ALT increased in the early periods only. Liver AST activity was enhanced after 12 h of exposure, and after 48 h its activity reduced significantly. Hepatic histological analysis presented hepatocyte vacuolation after 192 h of exposure to herbicide concentration of 1.0 mg/L. However, in recovery period of 192 h, hepatocyte vacuolation was observed in both clomazone concentration tested. Most of the metabolic disorders did not persist after recovery period, except to liver AST and ALT activities. The present work shows that clomazone concentrations used in rice fields can cause changes in metabolic state of the fish, which are of moderate intensity and generally reversible. |
publishDate |
2004 |
dc.date.issued.fl_str_mv |
2004-09-10 |
dc.date.accessioned.fl_str_mv |
2017-04-24 |
dc.date.available.fl_str_mv |
2017-04-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BIN, Márcia Crestani. Effects of clomazone herbicide on metabolic and antioxidant parameters in silver catfish(Rhamdia quelen). 2004. 109 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2004. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/11171 |
identifier_str_mv |
BIN, Márcia Crestani. Effects of clomazone herbicide on metabolic and antioxidant parameters in silver catfish(Rhamdia quelen). 2004. 109 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2004. |
url |
http://repositorio.ufsm.br/handle/1/11171 |
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por |
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400 500 300 500 500 |
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Universidade Federal de Santa Maria |
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UFSM |
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Bioquímica |
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Universidade Federal de Santa Maria |
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