Efeito antitumoral do extrato de carotenoides e biomassa de microalgas Scenedesmus obliquus em células de melanoma: envolvimento da sinalização purinérgica
Ano de defesa: | 2023 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
|
Departamento: |
Bioquímica
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/29637 |
Resumo: | Cutaneous Melanoma (CM) is an aggressive and metastatic tumor, resulting in low survival and high mortality rates. Despite significant advances in therapeutics, the disponible drugs are often undefective and related to resistance in the long term. Purinergic signaling has emerged as a potential pathway to cancer therapy due to its involvement in cell communication, proliferation, differentiation, and apoptosis. Actually, carotenoids from microalgae have been investigated in anti-melanoma therapy, due to safety and acceptable clinical tolerability. Thus, the objective of this work is to investigate the anti-melanogenic effect of carotenoids (CA) and total biomass (BM) from microalgae Scenedesmus obliquus on two cell lines (A375 and B16- F10) of cutaneous melanoma. Cells were cultivated under ideal conditions and treated with 10, 25, 50, and 100 μM of CA and BM separately for 24 hours. The effects of the compounds on viability, colony formation capacity, oxidant status, and purinergic signaling were verified. Our results showed that CA and BM decreased cell viability at IC50% 24,29 μM and 74,85 μM in B16-F10 and 73,93 μM and 127,80 μM in A375 lineages respectively. Also, both CA and BM isolated and associated with chemotherapy cisplatin (CIS), positively modulate ATP levels in levels in B16-F10 and A375 lineages through P2X7 manner and leading to cell apoptosis and immunoregulation through activation of A2A receptors and CD73 blockage. Together, the results reveal that the CA and BM from S. obliquus display an anti-melanogenic effect, inhibiting melanoma cells and providing the basis for therapeutic strategies for melanoma. |
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2023-07-05T15:31:09Z2023-07-05T15:31:09Z2023-05-19http://repositorio.ufsm.br/handle/1/29637Cutaneous Melanoma (CM) is an aggressive and metastatic tumor, resulting in low survival and high mortality rates. Despite significant advances in therapeutics, the disponible drugs are often undefective and related to resistance in the long term. Purinergic signaling has emerged as a potential pathway to cancer therapy due to its involvement in cell communication, proliferation, differentiation, and apoptosis. Actually, carotenoids from microalgae have been investigated in anti-melanoma therapy, due to safety and acceptable clinical tolerability. Thus, the objective of this work is to investigate the anti-melanogenic effect of carotenoids (CA) and total biomass (BM) from microalgae Scenedesmus obliquus on two cell lines (A375 and B16- F10) of cutaneous melanoma. Cells were cultivated under ideal conditions and treated with 10, 25, 50, and 100 μM of CA and BM separately for 24 hours. The effects of the compounds on viability, colony formation capacity, oxidant status, and purinergic signaling were verified. Our results showed that CA and BM decreased cell viability at IC50% 24,29 μM and 74,85 μM in B16-F10 and 73,93 μM and 127,80 μM in A375 lineages respectively. Also, both CA and BM isolated and associated with chemotherapy cisplatin (CIS), positively modulate ATP levels in levels in B16-F10 and A375 lineages through P2X7 manner and leading to cell apoptosis and immunoregulation through activation of A2A receptors and CD73 blockage. Together, the results reveal that the CA and BM from S. obliquus display an anti-melanogenic effect, inhibiting melanoma cells and providing the basis for therapeutic strategies for melanoma.O Câncer da Pele (CP) é caracterizado pelo crescimento anormal e descontrolado das células que formam a pele, dividindo-se basicamente em dois tipos: o câncer de pele melanoma e o câncer de pele não melanoma. O Melanoma Cutâneo (MC) é um câncer extremamente agressivo, com baixa sobrevida e alta mortalidade. Muitos pacientes diagnosticados tardiamente acabam apresentando metástase e resistência às drogas quimioterápicas utilizadas, o que acaba desfavorecendo os tratamentos. Devido a isso, a busca por novos agentes terapêuticos é de extrema necessidade. Atualmente, compostos de origem natural têm sido amplamente estudados devido a uma infinidade de propriedades biológicas. Entre estes compostos encontram-se os carotenoides, que possuem um amplo espectro de atividades farmacológicas e efeitos antitumorais em alguns tipos de câncer. Assim, o objetivo deste trabalho é investigar o efeito anti-melanogênico dos carotenoides (CA) e da biomassa total (BM) da microalga Scenedesmus obliquus em duas linhas celulares (A375 e B16-F10) de melanoma cutâneo. As células foram cultivadas em condições ideais e tratadas com 10, 25, 50 e 100 μM de CA e BM separadamente durante 24 horas. Foram verificados os efeitos dos compostos sobre a viabilidade, a capacidade de formação de colônias, o estado oxidante e a sinalização purinérgica. Os nossos resultados mostraram que o CA e a BM diminuíram a viabilidade celular com IC50% 24,29 μM e 74,85 μM em B16-F10 e 73,93 μM e 127,80 μM em linhagens A375, respectivamente. Observamos um efeito pró-oxidante em ambos os tratamentos nas duas linhagens celulares, medidos através da intensidade de fluorescência (DPPH) e do óxido nítrico (ON). Além disso, tanto o CA como o BM isolados e associados ao quimioterápico cisplatina (CIS), modularam positivamente os níveis de ATP nas linhagens B16-F10 e A375 através da via P2X7 e conduzem à apoptose celular e à imunorregulação através da ativação dos receptores A2A e do bloqueio do CD73. Em conjunto, os resultados revelam que o CA e BM de S. obliquus apresentam um efeito anti-melanogênico, inibindo as células de melanoma e fornecendo a base para estratégias terapêuticas para o melanoma.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessCâncer de peleCarotenoidesBiomassa totalAdenosina trifosfatoSistema purinérgicoEstresse oxidativoCarotenoidsAdenosineATPSkin cancerPurinergic systemCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEfeito antitumoral do extrato de carotenoides e biomassa de microalgas Scenedesmus obliquus em células de melanoma: envolvimento da sinalização purinérgicaAntitumor effect of carotenoid extract and Scenedesmus Obliquus microalgae biomass in melanoma cells: involvement of purinergic signalinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisChitolina, Maria Rosahttp://lattes.cnpq.br/4401319386725357Bottari, Nathieli BianchinMelazzo, CinthiaBaldissarelli, Jucimarahttp://lattes.cnpq.br/7740338714165501Jesus, Loren Stephanie Borba de2008000000026006006006006006003a9dc4db-b442-4eab-840e-5158c5a64c7c33f765a1-0a58-40bd-843e-49968fec207e1fbe08d8-99cf-4fff-95d7-75891e5b718411b6196b-b9a3-4898-ba98-d87a4f17d337516adf5e-0aa1-4efa-8993-dca44507d10freponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGBT_2023_JESUS_LOREN.pdfDIS_PPGBT_2023_JESUS_LOREN.pdfDissertaçãoapplication/pdf1864239http://repositorio.ufsm.br/bitstream/1/29637/1/DIS_PPGBT_2023_JESUS_LOREN.pdf329a2ea1b5cd385ea81afdd50f64a8f3MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Efeito antitumoral do extrato de carotenoides e biomassa de microalgas Scenedesmus obliquus em células de melanoma: envolvimento da sinalização purinérgica |
dc.title.alternative.eng.fl_str_mv |
Antitumor effect of carotenoid extract and Scenedesmus Obliquus microalgae biomass in melanoma cells: involvement of purinergic signaling |
title |
Efeito antitumoral do extrato de carotenoides e biomassa de microalgas Scenedesmus obliquus em células de melanoma: envolvimento da sinalização purinérgica |
spellingShingle |
Efeito antitumoral do extrato de carotenoides e biomassa de microalgas Scenedesmus obliquus em células de melanoma: envolvimento da sinalização purinérgica Jesus, Loren Stephanie Borba de Câncer de pele Carotenoides Biomassa total Adenosina trifosfato Sistema purinérgico Estresse oxidativo Carotenoids Adenosine ATP Skin cancer Purinergic system CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Efeito antitumoral do extrato de carotenoides e biomassa de microalgas Scenedesmus obliquus em células de melanoma: envolvimento da sinalização purinérgica |
title_full |
Efeito antitumoral do extrato de carotenoides e biomassa de microalgas Scenedesmus obliquus em células de melanoma: envolvimento da sinalização purinérgica |
title_fullStr |
Efeito antitumoral do extrato de carotenoides e biomassa de microalgas Scenedesmus obliquus em células de melanoma: envolvimento da sinalização purinérgica |
title_full_unstemmed |
Efeito antitumoral do extrato de carotenoides e biomassa de microalgas Scenedesmus obliquus em células de melanoma: envolvimento da sinalização purinérgica |
title_sort |
Efeito antitumoral do extrato de carotenoides e biomassa de microalgas Scenedesmus obliquus em células de melanoma: envolvimento da sinalização purinérgica |
author |
Jesus, Loren Stephanie Borba de |
author_facet |
Jesus, Loren Stephanie Borba de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Chitolina, Maria Rosa |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4401319386725357 |
dc.contributor.advisor-co1.fl_str_mv |
Bottari, Nathieli Bianchin |
dc.contributor.referee1.fl_str_mv |
Melazzo, Cinthia |
dc.contributor.referee2.fl_str_mv |
Baldissarelli, Jucimara |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7740338714165501 |
dc.contributor.author.fl_str_mv |
Jesus, Loren Stephanie Borba de |
contributor_str_mv |
Chitolina, Maria Rosa Bottari, Nathieli Bianchin Melazzo, Cinthia Baldissarelli, Jucimara |
dc.subject.por.fl_str_mv |
Câncer de pele Carotenoides Biomassa total Adenosina trifosfato Sistema purinérgico Estresse oxidativo |
topic |
Câncer de pele Carotenoides Biomassa total Adenosina trifosfato Sistema purinérgico Estresse oxidativo Carotenoids Adenosine ATP Skin cancer Purinergic system CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Carotenoids Adenosine ATP Skin cancer Purinergic system |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Cutaneous Melanoma (CM) is an aggressive and metastatic tumor, resulting in low survival and high mortality rates. Despite significant advances in therapeutics, the disponible drugs are often undefective and related to resistance in the long term. Purinergic signaling has emerged as a potential pathway to cancer therapy due to its involvement in cell communication, proliferation, differentiation, and apoptosis. Actually, carotenoids from microalgae have been investigated in anti-melanoma therapy, due to safety and acceptable clinical tolerability. Thus, the objective of this work is to investigate the anti-melanogenic effect of carotenoids (CA) and total biomass (BM) from microalgae Scenedesmus obliquus on two cell lines (A375 and B16- F10) of cutaneous melanoma. Cells were cultivated under ideal conditions and treated with 10, 25, 50, and 100 μM of CA and BM separately for 24 hours. The effects of the compounds on viability, colony formation capacity, oxidant status, and purinergic signaling were verified. Our results showed that CA and BM decreased cell viability at IC50% 24,29 μM and 74,85 μM in B16-F10 and 73,93 μM and 127,80 μM in A375 lineages respectively. Also, both CA and BM isolated and associated with chemotherapy cisplatin (CIS), positively modulate ATP levels in levels in B16-F10 and A375 lineages through P2X7 manner and leading to cell apoptosis and immunoregulation through activation of A2A receptors and CD73 blockage. Together, the results reveal that the CA and BM from S. obliquus display an anti-melanogenic effect, inhibiting melanoma cells and providing the basis for therapeutic strategies for melanoma. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-07-05T15:31:09Z |
dc.date.available.fl_str_mv |
2023-07-05T15:31:09Z |
dc.date.issued.fl_str_mv |
2023-05-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://repositorio.ufsm.br/handle/1/29637 |
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http://repositorio.ufsm.br/handle/1/29637 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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200800000002 |
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600 600 600 600 600 600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Bioquímica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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