Produção biotecnólogica de ácido succínico a partir de casca de arroz
Ano de defesa: | 2013 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Química
|
Departamento: |
Química
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/4260 |
Resumo: | Rice husk is a subproduct of the food industry, rich in carbohydrates, which can be partially fractionated and converted into fermentable sugars. In this work, it was investigated the best conditions for the conversion of the residual biomass, rice husks, into succinic acid, an important start molecule for the synthesis of the chemo-pharmaceutical industry. With the goal of the separation of lignin and transformation of cellulose and hemicellulose into sugars, the rice husks were submitted, initially, to acid hydrolysis, in autoclave and in pressurized polytetrafluoroethylene reactor. The hydrolysis conditions were optimized by factorial design for the pressurized acid hydrolysis; temperature, time and acid catalyst concentration (HCl or H2SO4) were evaluated. For the acid hydrolysis in autoclave, it was optimized the ratio rice husks mass: acid volume, time and concentration of HCl or H2SO4. It was observed that the sugar production by using autoclave was lower than by the pressurized hydrolysis system, needing further concentration of the hydrolysate for the subsequent fermentation step. The best results were obtained with the polytetrafluoroethylene reactor, by 59 bar, with HCl 0,26 mol L-1, at 175°C and reaction time of 46 min, yielding 19.0 g L- 1 of glucose and 3.01 g L- 1 of xylose. The efficiency of different detoxification methods of the hydrolyzed rice husk were evaluated; the combined method of pH adjustment plus adsorption on active carbon was the most effective by eliminating inhibitors, without appreciable reduction of the sugar concentration. The detoxified hydrolysate was sterilized and adjusted at pH 7 and fermented with A. succinogenes at 37 ° C, in anaerobic medium, occurring the conversion of the two main monosaccharides, glucose and xylose, into succinic acid. The nutrient concentration and the agitation rate of the medium were also optimized by factorial design. As a result, after 54 h of static fermentation, the hydrolysate was supplemented with 8.40 g L-1 yeast extract and 1.40 g L -1 of NaHCO3, to yield 59.9% succinic acid. Almost all of the sugar at this time was consumed and converted to succinic acid; at the same time, acetic and formic acid are formed, but, in low concentrations related to the production of succinic acid, not compromising the yield of the process. For the succinic acid extraction and purification, the fermentate was submitted to the solid phase extraction procedure; cartridges with different extraction phases were tested, and among them, the ion exchange one was the only effective, with recoveries up to 96%. After solid phase extraction, the eluted solution, containing 12.05 g L- 1 succcinic acid, was lyophilized, and crystals of succinic acid with 80.7% (m m- 1) were obtained. The raw material used in the bioprocess has no commercial value, representing a zero cost carbon source, which reveals itself adequate to the succinic acid production by fermentation with A. succinogenes, after hydrolysis. The use of the residual rice husk can contribute to the mitigation of the environmental impact resulting from the illegal discharge in the environment. |
id |
UFSM_51fa6f9ec70d05e2273dae2c0eccb87c |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/4260 |
network_acronym_str |
UFSM |
network_name_str |
Biblioteca Digital de Teses e Dissertações do UFSM |
repository_id_str |
|
spelling |
2017-05-122017-05-122013-12-16BEVILAQUA, Daiane Balconi. Biotecnological production of succinic acid from rice husks. 2013. 124 f. Tese (Doutorado em Química) - Universidade Federal de Santa Maria, Santa Maria, 2013.http://repositorio.ufsm.br/handle/1/4260Rice husk is a subproduct of the food industry, rich in carbohydrates, which can be partially fractionated and converted into fermentable sugars. In this work, it was investigated the best conditions for the conversion of the residual biomass, rice husks, into succinic acid, an important start molecule for the synthesis of the chemo-pharmaceutical industry. With the goal of the separation of lignin and transformation of cellulose and hemicellulose into sugars, the rice husks were submitted, initially, to acid hydrolysis, in autoclave and in pressurized polytetrafluoroethylene reactor. The hydrolysis conditions were optimized by factorial design for the pressurized acid hydrolysis; temperature, time and acid catalyst concentration (HCl or H2SO4) were evaluated. For the acid hydrolysis in autoclave, it was optimized the ratio rice husks mass: acid volume, time and concentration of HCl or H2SO4. It was observed that the sugar production by using autoclave was lower than by the pressurized hydrolysis system, needing further concentration of the hydrolysate for the subsequent fermentation step. The best results were obtained with the polytetrafluoroethylene reactor, by 59 bar, with HCl 0,26 mol L-1, at 175°C and reaction time of 46 min, yielding 19.0 g L- 1 of glucose and 3.01 g L- 1 of xylose. The efficiency of different detoxification methods of the hydrolyzed rice husk were evaluated; the combined method of pH adjustment plus adsorption on active carbon was the most effective by eliminating inhibitors, without appreciable reduction of the sugar concentration. The detoxified hydrolysate was sterilized and adjusted at pH 7 and fermented with A. succinogenes at 37 ° C, in anaerobic medium, occurring the conversion of the two main monosaccharides, glucose and xylose, into succinic acid. The nutrient concentration and the agitation rate of the medium were also optimized by factorial design. As a result, after 54 h of static fermentation, the hydrolysate was supplemented with 8.40 g L-1 yeast extract and 1.40 g L -1 of NaHCO3, to yield 59.9% succinic acid. Almost all of the sugar at this time was consumed and converted to succinic acid; at the same time, acetic and formic acid are formed, but, in low concentrations related to the production of succinic acid, not compromising the yield of the process. For the succinic acid extraction and purification, the fermentate was submitted to the solid phase extraction procedure; cartridges with different extraction phases were tested, and among them, the ion exchange one was the only effective, with recoveries up to 96%. After solid phase extraction, the eluted solution, containing 12.05 g L- 1 succcinic acid, was lyophilized, and crystals of succinic acid with 80.7% (m m- 1) were obtained. The raw material used in the bioprocess has no commercial value, representing a zero cost carbon source, which reveals itself adequate to the succinic acid production by fermentation with A. succinogenes, after hydrolysis. The use of the residual rice husk can contribute to the mitigation of the environmental impact resulting from the illegal discharge in the environment.A casca de arroz é um subproduto da indústria de alimentos, rico em carboidratos, que pode ser fracionada e, parcialmente, convertida em açúcares fermentescíveis. Neste trabalho, investigou-se as melhores condições para a conversão da biomassa residual, casca de arroz, em ácido succínico, importante insumo para a síntese industrial farmoquímica. Com o objetivo de separação da lignina e transformação da celulose e da hemicelulose em açúcares, a casca de arroz foi submetida, inicialmente, à hidrólise ácida em autoclave e em reator de politetrafluoretileno, à pressão. As condições de hidrólise foram otimizadas através de planejamento fatorial, sendo avaliado na hidrólise ácida pressurizada, a influência da temperatura, do tempo e da concentração do catalisador ácido (HCl ou H2SO4); já, na hidrólise em autoclave, otimizou-se a relação massa de casca de arroz : volume de solução ácida, tempo e concentração de HCl ou H2SO4. Observou-se que a produção de açúcares em autoclave é inferior à do sistema de hidrólise à pressão, necessitando, inclusive, concentração do hidrolisado para utilização na etapa fermentativa. Os melhores resultados foram obtidos com o reator de politetrafluoretileno, à pressão de 59 bar, com 0,26 mol L-1 de HCl, temperatura de 175 °C e tempo de reação de 46 min, produzindo-se 19 g L-1 de glicose e 3,01 g L-1 de xilose. Avaliou-se a eficiência de diferentes métodos de destoxificação do hidrolisado de casca de arroz, sendo o método combinado, de ajuste de pH seguido de adsorção em carvão ativado, o mais eficaz na eliminação de inibidores, sem redução apreciável da concentração de açúcares. O hidrolisado destoxificado foi esterilizado, ajustado a pH 7 e fermentado com A. succinogenes, à 37 ºC, em meio anaeróbio, ocorrendo a conversão dos monossacarídeos predominantes, glicose e xilose, em ácido succínico. A concentração dos nutrientes e a velocidade de agitação do meio também foram otimizadas por meio de planejamento fatorial. Após 54 h de fermentação estática do hidrolisado, suplementado com 8,40 g L-1 de extrato de levedura e 1,40 g L-1 de NaHCO3, o rendimento em ácido succínico foi de 59,9%. Praticamente, toda a concentração de açúcar é consumida neste tempo e convertida em ácido succínico; simultaneamente, formam-se ácido acético e fórmico, porém, em baixas concentrações em relação à produção de ácido succínico, não comprometendo o rendimento do processo. Para a extração e purificação do ácido succínico, o fermentado foi submetido ao procedimento de extração em fase sólida; cartuchos com diferentes fases extratoras foram testados, e, dentre eles, somente o de troca iônica se mostrou efetivo, com recuperação de até 96,0%. Após a extração, o eluido da extração em fase sólida, contendo 12,0 g L-1 de ácido succínico foi liofilizado, obtendo-se cristais com pureza de 80,7% (m m-1). A matéria-prima utilizada no bioprocesso, casca de arroz, não tem valor comercial, representando fonte de carbono de custo zero, que se revelou adequada à produção de ácido succínico por meio de fermentação com A. succinogenes, após hidrólise. O aproveitamento da casca de arroz residual pode contribuir para a mitigação do impacto ambiental resultante da disposição ilegal no ambiente.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em QuímicaUFSMBRQuímicaCasca de arrozHidrólise ácida pressurizadaA. succinogenesÁcido succínicoOtimização do processoRice husksPressurized acid hydrolysisA. succinogenesSuccinic acidOptimization processCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICAProdução biotecnólogica de ácido succínico a partir de casca de arrozBiotecnological production of succinic acid from rice husksinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisMartins, Ayrton Figueiredohttp://lattes.cnpq.br/2113532494494821Oliveira, Elizangela Gonçalves dehttp://lattes.cnpq.br/1578951812092017Carissimi, Elvishttp://lattes.cnpq.br/4642912749433316Dallago, Rogério Marcoshttp://lattes.cnpq.br/3006526142468362Richards, Neila Silvia Pereira dos Santoshttp://lattes.cnpq.br/0618653776990780http://lattes.cnpq.br/3787674861849653Bevilaqua, Daiane Balconi100600000000400300300300300300500ade6000d-a348-4d49-a531-6500a65a153a486bc091-1e85-4d81-bbae-50f40b6604ad3778f147-7940-482c-b415-b28faf78b23ce17fa918-e6fc-4cc7-95ff-b1b504fb16a424ded0f7-6d99-4c39-997d-0e6db3f4407e17a21f77-88b4-4d77-8396-049941753c69info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALBEVILAQUA, DAIANE BALCONI.pdfapplication/pdf4426288http://repositorio.ufsm.br/bitstream/1/4260/1/BEVILAQUA%2c%20DAIANE%20BALCONI.pdf46dd0dd78299f450df24bd7f0585d357MD51TEXTBEVILAQUA, DAIANE BALCONI.pdf.txtBEVILAQUA, DAIANE BALCONI.pdf.txtExtracted texttext/plain195118http://repositorio.ufsm.br/bitstream/1/4260/2/BEVILAQUA%2c%20DAIANE%20BALCONI.pdf.txt03f061d09b5170a9922d67cfcd3fea91MD52THUMBNAILBEVILAQUA, DAIANE BALCONI.pdf.jpgBEVILAQUA, DAIANE BALCONI.pdf.jpgIM Thumbnailimage/jpeg4535http://repositorio.ufsm.br/bitstream/1/4260/3/BEVILAQUA%2c%20DAIANE%20BALCONI.pdf.jpg316b2723cc4bf7f8d79f9fb5c14e4242MD531/42602023-05-11 14:34:31.194oai:repositorio.ufsm.br:1/4260Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-05-11T17:34:31Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Produção biotecnólogica de ácido succínico a partir de casca de arroz |
dc.title.alternative.eng.fl_str_mv |
Biotecnological production of succinic acid from rice husks |
title |
Produção biotecnólogica de ácido succínico a partir de casca de arroz |
spellingShingle |
Produção biotecnólogica de ácido succínico a partir de casca de arroz Bevilaqua, Daiane Balconi Casca de arroz Hidrólise ácida pressurizada A. succinogenes Ácido succínico Otimização do processo Rice husks Pressurized acid hydrolysis A. succinogenes Succinic acid Optimization process CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Produção biotecnólogica de ácido succínico a partir de casca de arroz |
title_full |
Produção biotecnólogica de ácido succínico a partir de casca de arroz |
title_fullStr |
Produção biotecnólogica de ácido succínico a partir de casca de arroz |
title_full_unstemmed |
Produção biotecnólogica de ácido succínico a partir de casca de arroz |
title_sort |
Produção biotecnólogica de ácido succínico a partir de casca de arroz |
author |
Bevilaqua, Daiane Balconi |
author_facet |
Bevilaqua, Daiane Balconi |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Martins, Ayrton Figueiredo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2113532494494821 |
dc.contributor.referee1.fl_str_mv |
Oliveira, Elizangela Gonçalves de |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1578951812092017 |
dc.contributor.referee2.fl_str_mv |
Carissimi, Elvis |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/4642912749433316 |
dc.contributor.referee3.fl_str_mv |
Dallago, Rogério Marcos |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/3006526142468362 |
dc.contributor.referee4.fl_str_mv |
Richards, Neila Silvia Pereira dos Santos |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/0618653776990780 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3787674861849653 |
dc.contributor.author.fl_str_mv |
Bevilaqua, Daiane Balconi |
contributor_str_mv |
Martins, Ayrton Figueiredo Oliveira, Elizangela Gonçalves de Carissimi, Elvis Dallago, Rogério Marcos Richards, Neila Silvia Pereira dos Santos |
dc.subject.por.fl_str_mv |
Casca de arroz Hidrólise ácida pressurizada A. succinogenes Ácido succínico Otimização do processo |
topic |
Casca de arroz Hidrólise ácida pressurizada A. succinogenes Ácido succínico Otimização do processo Rice husks Pressurized acid hydrolysis A. succinogenes Succinic acid Optimization process CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Rice husks Pressurized acid hydrolysis A. succinogenes Succinic acid Optimization process |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
Rice husk is a subproduct of the food industry, rich in carbohydrates, which can be partially fractionated and converted into fermentable sugars. In this work, it was investigated the best conditions for the conversion of the residual biomass, rice husks, into succinic acid, an important start molecule for the synthesis of the chemo-pharmaceutical industry. With the goal of the separation of lignin and transformation of cellulose and hemicellulose into sugars, the rice husks were submitted, initially, to acid hydrolysis, in autoclave and in pressurized polytetrafluoroethylene reactor. The hydrolysis conditions were optimized by factorial design for the pressurized acid hydrolysis; temperature, time and acid catalyst concentration (HCl or H2SO4) were evaluated. For the acid hydrolysis in autoclave, it was optimized the ratio rice husks mass: acid volume, time and concentration of HCl or H2SO4. It was observed that the sugar production by using autoclave was lower than by the pressurized hydrolysis system, needing further concentration of the hydrolysate for the subsequent fermentation step. The best results were obtained with the polytetrafluoroethylene reactor, by 59 bar, with HCl 0,26 mol L-1, at 175°C and reaction time of 46 min, yielding 19.0 g L- 1 of glucose and 3.01 g L- 1 of xylose. The efficiency of different detoxification methods of the hydrolyzed rice husk were evaluated; the combined method of pH adjustment plus adsorption on active carbon was the most effective by eliminating inhibitors, without appreciable reduction of the sugar concentration. The detoxified hydrolysate was sterilized and adjusted at pH 7 and fermented with A. succinogenes at 37 ° C, in anaerobic medium, occurring the conversion of the two main monosaccharides, glucose and xylose, into succinic acid. The nutrient concentration and the agitation rate of the medium were also optimized by factorial design. As a result, after 54 h of static fermentation, the hydrolysate was supplemented with 8.40 g L-1 yeast extract and 1.40 g L -1 of NaHCO3, to yield 59.9% succinic acid. Almost all of the sugar at this time was consumed and converted to succinic acid; at the same time, acetic and formic acid are formed, but, in low concentrations related to the production of succinic acid, not compromising the yield of the process. For the succinic acid extraction and purification, the fermentate was submitted to the solid phase extraction procedure; cartridges with different extraction phases were tested, and among them, the ion exchange one was the only effective, with recoveries up to 96%. After solid phase extraction, the eluted solution, containing 12.05 g L- 1 succcinic acid, was lyophilized, and crystals of succinic acid with 80.7% (m m- 1) were obtained. The raw material used in the bioprocess has no commercial value, representing a zero cost carbon source, which reveals itself adequate to the succinic acid production by fermentation with A. succinogenes, after hydrolysis. The use of the residual rice husk can contribute to the mitigation of the environmental impact resulting from the illegal discharge in the environment. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-12-16 |
dc.date.accessioned.fl_str_mv |
2017-05-12 |
dc.date.available.fl_str_mv |
2017-05-12 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BEVILAQUA, Daiane Balconi. Biotecnological production of succinic acid from rice husks. 2013. 124 f. Tese (Doutorado em Química) - Universidade Federal de Santa Maria, Santa Maria, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/4260 |
identifier_str_mv |
BEVILAQUA, Daiane Balconi. Biotecnological production of succinic acid from rice husks. 2013. 124 f. Tese (Doutorado em Química) - Universidade Federal de Santa Maria, Santa Maria, 2013. |
url |
http://repositorio.ufsm.br/handle/1/4260 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
100600000000 |
dc.relation.confidence.fl_str_mv |
400 300 300 300 300 300 500 |
dc.relation.authority.fl_str_mv |
ade6000d-a348-4d49-a531-6500a65a153a 486bc091-1e85-4d81-bbae-50f40b6604ad 3778f147-7940-482c-b415-b28faf78b23c e17fa918-e6fc-4cc7-95ff-b1b504fb16a4 24ded0f7-6d99-4c39-997d-0e6db3f4407e 17a21f77-88b4-4d77-8396-049941753c69 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Química |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Química |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações do UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Biblioteca Digital de Teses e Dissertações do UFSM |
collection |
Biblioteca Digital de Teses e Dissertações do UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/4260/1/BEVILAQUA%2c%20DAIANE%20BALCONI.pdf http://repositorio.ufsm.br/bitstream/1/4260/2/BEVILAQUA%2c%20DAIANE%20BALCONI.pdf.txt http://repositorio.ufsm.br/bitstream/1/4260/3/BEVILAQUA%2c%20DAIANE%20BALCONI.pdf.jpg |
bitstream.checksum.fl_str_mv |
46dd0dd78299f450df24bd7f0585d357 03f061d09b5170a9922d67cfcd3fea91 316b2723cc4bf7f8d79f9fb5c14e4242 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1793239991061577728 |