Tabernaemontana catharinensis A. DC: estudo dos metabólitos secundários, atividade biológica, cultivo in vitro e quantificação por RMN de alcaloides majoritarios
Ano de defesa: | 2022 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Química
|
Departamento: |
Química
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/26384 |
Resumo: | The phytochemical investigation of the fractions from the leaves of Tabernaemontana catharinensis (Apocynaceae, popularly known as cobrina), led to the isolation of seven componds, three steroids: stigmasterol, β-sitosterol acetate and glycosylated β-sitosterol, a triterpene: betulinic acid, a flavonoid: quercitin, and two phenolic compounds: chlorogenic acid and caffeic acid. Derivation reactions were performed for the alkaloids ethyl apovincamine, voachalotine and affinisine, obtaining seven derivatives. The fractions and isolated compounds showed promising antibacterial, antifungal and antibiofilm activity against the tested strains. The derivatives obtained were more active against fungi of the genus Candida. The alkaloid affinisin was strongly active against the tumor cell lines tested, and in many cases it was more active than the chemotherapeutic doxorubicin, in addition to having cytocidal and cytotoxic potential and high levels of selectivity. Protocols for asepsis and in vitro proliferation of T. catharinensis seeds were defined, where cultivation in solid and liquid culture medium was possible, the latter being reported for the first time for the genus. After in vitro cultivation and obtaining large populations of seedlings, microextractions were performed in order to obtain acidic, basic and neutral fractions that were quantified by NMR, making it possible to quantify four alkaloids in the acidic fractions and two in the basic fractions. The present work adds great results to the studies of the species T. catharinensis A.DC., since this species has great ethnopharmacological applicability, many of which still do not have scientific records, but are kept in popular culture. |
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2022-10-07T15:14:58Z2022-10-07T15:14:58Z2022-08-25http://repositorio.ufsm.br/handle/1/26384The phytochemical investigation of the fractions from the leaves of Tabernaemontana catharinensis (Apocynaceae, popularly known as cobrina), led to the isolation of seven componds, three steroids: stigmasterol, β-sitosterol acetate and glycosylated β-sitosterol, a triterpene: betulinic acid, a flavonoid: quercitin, and two phenolic compounds: chlorogenic acid and caffeic acid. Derivation reactions were performed for the alkaloids ethyl apovincamine, voachalotine and affinisine, obtaining seven derivatives. The fractions and isolated compounds showed promising antibacterial, antifungal and antibiofilm activity against the tested strains. The derivatives obtained were more active against fungi of the genus Candida. The alkaloid affinisin was strongly active against the tumor cell lines tested, and in many cases it was more active than the chemotherapeutic doxorubicin, in addition to having cytocidal and cytotoxic potential and high levels of selectivity. Protocols for asepsis and in vitro proliferation of T. catharinensis seeds were defined, where cultivation in solid and liquid culture medium was possible, the latter being reported for the first time for the genus. After in vitro cultivation and obtaining large populations of seedlings, microextractions were performed in order to obtain acidic, basic and neutral fractions that were quantified by NMR, making it possible to quantify four alkaloids in the acidic fractions and two in the basic fractions. The present work adds great results to the studies of the species T. catharinensis A.DC., since this species has great ethnopharmacological applicability, many of which still do not have scientific records, but are kept in popular culture.A investigação fitoquímica das frações, oriundas das folhas de Tabernaemontana catharinensis (Apocynaceae, conhecida popularmente como cobrina), levou ao isolamento de sete compostos, três esteroides: estigmasterol, acetato de β-sitosterol e β-sitosterol glicosilado, um triterpeno: ácido betulínico, um flavonóide: quercitina além de dois compostos fenólicos: ácido clorogênico e ácido cafeico. Foram realizadas reações de derivatização para os alcaloides etil apovincamina, voachalotina e affinisina, obtendo-se sete derivados. As frações e compostos isolados apresentaram atividade antibacteriana, antifúngica e antibiofilme promissoras, contra as cepas testadas. Os derivados obtidos mostraram-se mais ativos contra fungos do gênero Candida. O alcaloide affinisina mostrou fortemente ativo frente às linhagens celulares tumorais testadas, sendo que em muitos casos mostrou-se mais ativa que o quimioterápico doxorrubicina, além disso apresentou potencial citócido e citotóxico e altos índices de seletividade. Foram definidos protocolos de assepsia e proliferação in vitro de sementes de T. catharinensis, onde foi possível o cultivo em meio de cultura sólido e líquido, sendo este último relatado pela primeira vez para o gênero. Após o cultivo in vitro e a obtenção de grandes populações de plântulas, foram realizadas microextrações a fim de obter frações ácidas, básicas e neutras que foram quantificadas por RMN, sendo possível quantificar quatro alcaloides nas frações ácidas e dois nas frações básicas. O presente trabalho agrega grandes resultados aos estudos da espécie T. catharinensis A.DC., visto que esta espécie tem grande aplicabilidade etnofarmacológica das quais muitas ainda não possuem registros científicos, porém são mantidos na cultura popular.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em QuímicaUFSMBrasilQuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessTabernaemontana catharinensisAlcaloide indólicoAtividade antimicrobianaCultivo in vitroQuantificação por RMNIndole alkaloidAntimicrobial activityIn vitro cultureQuantification by NMRCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICATabernaemontana catharinensis A. DC: estudo dos metabólitos secundários, atividade biológica, cultivo in vitro e quantificação por RMN de alcaloides majoritariosTabernaemontana catharinensis A. DC: study of secondary metabolites, biological activity, in vitro culture and NMR quantification of major alkaloidsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisMorel, Ademir Fariashttp://lattes.cnpq.br/3554994385525333Tiburski Neto, AlexandreMostardeiro, Marco AurélioDalcol, Ionara IrionSchumacher, Ricardo Fredericohttp://lattes.cnpq.br/3296035379633029Reis, Frederico Luiz1006000000006006006006006006006008c99befa-f1b3-4afb-98b8-7fab46c3d49312d17253-66bc-4e91-a246-cade1d323cae5611c87f-4542-48ff-857c-81cda32900e1cc343f59-e39b-4e1a-859d-a2a9f0e4037f11b367fb-0eed-426e-9ab5-e42e52a8d0986c8d4d2c-6e7a-4fa7-8f85-f8e27a24863breponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMLICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/26384/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53ORIGINALTES_PPGQUÍMICA_2022_REIS_FREDERICO.pdfTES_PPGQUÍMICA_2022_REIS_FREDERICO.pdfTese de doutoradoapplication/pdf5510828http://repositorio.ufsm.br/bitstream/1/26384/1/TES_PPGQU%c3%8dMICA_2022_REIS_FREDERICO.pdf6ccfe521aa64710ae8380075424ea768MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/26384/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD521/263842022-10-07 12:14:58.222oai:repositorio.ufsm.br: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 Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-10-07T15:14:58Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Tabernaemontana catharinensis A. DC: estudo dos metabólitos secundários, atividade biológica, cultivo in vitro e quantificação por RMN de alcaloides majoritarios |
dc.title.alternative.eng.fl_str_mv |
Tabernaemontana catharinensis A. DC: study of secondary metabolites, biological activity, in vitro culture and NMR quantification of major alkaloids |
title |
Tabernaemontana catharinensis A. DC: estudo dos metabólitos secundários, atividade biológica, cultivo in vitro e quantificação por RMN de alcaloides majoritarios |
spellingShingle |
Tabernaemontana catharinensis A. DC: estudo dos metabólitos secundários, atividade biológica, cultivo in vitro e quantificação por RMN de alcaloides majoritarios Reis, Frederico Luiz Tabernaemontana catharinensis Alcaloide indólico Atividade antimicrobiana Cultivo in vitro Quantificação por RMN Indole alkaloid Antimicrobial activity In vitro culture Quantification by NMR CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Tabernaemontana catharinensis A. DC: estudo dos metabólitos secundários, atividade biológica, cultivo in vitro e quantificação por RMN de alcaloides majoritarios |
title_full |
Tabernaemontana catharinensis A. DC: estudo dos metabólitos secundários, atividade biológica, cultivo in vitro e quantificação por RMN de alcaloides majoritarios |
title_fullStr |
Tabernaemontana catharinensis A. DC: estudo dos metabólitos secundários, atividade biológica, cultivo in vitro e quantificação por RMN de alcaloides majoritarios |
title_full_unstemmed |
Tabernaemontana catharinensis A. DC: estudo dos metabólitos secundários, atividade biológica, cultivo in vitro e quantificação por RMN de alcaloides majoritarios |
title_sort |
Tabernaemontana catharinensis A. DC: estudo dos metabólitos secundários, atividade biológica, cultivo in vitro e quantificação por RMN de alcaloides majoritarios |
author |
Reis, Frederico Luiz |
author_facet |
Reis, Frederico Luiz |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Morel, Ademir Farias |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3554994385525333 |
dc.contributor.referee1.fl_str_mv |
Tiburski Neto, Alexandre |
dc.contributor.referee2.fl_str_mv |
Mostardeiro, Marco Aurélio |
dc.contributor.referee3.fl_str_mv |
Dalcol, Ionara Irion |
dc.contributor.referee4.fl_str_mv |
Schumacher, Ricardo Frederico |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3296035379633029 |
dc.contributor.author.fl_str_mv |
Reis, Frederico Luiz |
contributor_str_mv |
Morel, Ademir Farias Tiburski Neto, Alexandre Mostardeiro, Marco Aurélio Dalcol, Ionara Irion Schumacher, Ricardo Frederico |
dc.subject.por.fl_str_mv |
Tabernaemontana catharinensis Alcaloide indólico Atividade antimicrobiana Cultivo in vitro Quantificação por RMN |
topic |
Tabernaemontana catharinensis Alcaloide indólico Atividade antimicrobiana Cultivo in vitro Quantificação por RMN Indole alkaloid Antimicrobial activity In vitro culture Quantification by NMR CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Indole alkaloid Antimicrobial activity In vitro culture Quantification by NMR |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
The phytochemical investigation of the fractions from the leaves of Tabernaemontana catharinensis (Apocynaceae, popularly known as cobrina), led to the isolation of seven componds, three steroids: stigmasterol, β-sitosterol acetate and glycosylated β-sitosterol, a triterpene: betulinic acid, a flavonoid: quercitin, and two phenolic compounds: chlorogenic acid and caffeic acid. Derivation reactions were performed for the alkaloids ethyl apovincamine, voachalotine and affinisine, obtaining seven derivatives. The fractions and isolated compounds showed promising antibacterial, antifungal and antibiofilm activity against the tested strains. The derivatives obtained were more active against fungi of the genus Candida. The alkaloid affinisin was strongly active against the tumor cell lines tested, and in many cases it was more active than the chemotherapeutic doxorubicin, in addition to having cytocidal and cytotoxic potential and high levels of selectivity. Protocols for asepsis and in vitro proliferation of T. catharinensis seeds were defined, where cultivation in solid and liquid culture medium was possible, the latter being reported for the first time for the genus. After in vitro cultivation and obtaining large populations of seedlings, microextractions were performed in order to obtain acidic, basic and neutral fractions that were quantified by NMR, making it possible to quantify four alkaloids in the acidic fractions and two in the basic fractions. The present work adds great results to the studies of the species T. catharinensis A.DC., since this species has great ethnopharmacological applicability, many of which still do not have scientific records, but are kept in popular culture. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-10-07T15:14:58Z |
dc.date.available.fl_str_mv |
2022-10-07T15:14:58Z |
dc.date.issued.fl_str_mv |
2022-08-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/26384 |
url |
http://repositorio.ufsm.br/handle/1/26384 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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100600000000 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 600 600 |
dc.relation.authority.fl_str_mv |
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dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Química |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Química |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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