Produção de bio-hidrogênio a partir da casca de arroz via processo de fermentação escura
Ano de defesa: | 2021 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Tecnologia |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Engenharia Ambiental
|
Departamento: |
Engenharia Ambiental
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/24905 |
Resumo: | In the contemporary world, the growing global demand for energy, the reduction of non-renewable energy resources and the concern with the quality of the environment are noticeable. It is well known that the demand for hydrocarbons, which are the most energy source used today, exceeds the reserves forecast for the next decades. Furthermore, the environmental impact that their utilization has caused is responsible for global warming, destruction of the ozone layer, and disappearance of ecological systems in what some people call the sixth massive extinction. Thus, Bio-hydrogen (Bio-H2) can facilitate the decarbonization of certain carbon-emitting activities, as it is produced via dark fermentation, which is considered more viable, ecological and sustainable since it does not require an external energy source. Several types of lignocellulosic biomass can be used as a carbon source by microorganisms producing hydrogen (H2). In this sense, the objective of this work is to use rice husk (RH) and evaluate the production of H2 via the dark fermentation process under different types of inoculums. RH was characterized, prepared and submitted to the acid hydrolysis process with different concentrations of sulfuric acid and evaluated was performed based on the content of total sugars (TS) and at various concentrations of inhibitors. The selected hydrolyzate was then used as a substrate in the dark fermentation process, using two different types of sludge, anaerobic sludge granulation (ASG) and septic tank sludge (STS). The dark fermentation tests were carried out in a reactor with a substrate/inoculum ratio of 9 in the middle-temperature range of 35.8°C and neutral initial pH (6.5 - 7) and monitored for a period of 29 ± 1h approximately. The experimental condition that showed the highest total sugar recovery was the acid pretreatment to 1% v/v H2SO4. The recovery of TS in this condition was 201g kg RH-1, and inhibitor concentrations were less than the limit of quantification of 0.005 g L-1 for Furfural and 0.0005 g L-1 for 5-HMF, and the production and maximum yield of Bio-H2 were 5.37mL g RH-1 and 0.179 mL H2 h-1 g RH-1, respectively. Finally, it is concluded that the hydrolysate (1% v/v H2SO4) as substrate and ASG as inoculum exhibited adequate properties for the generation of Bio-H2 via the dark fermentation method, yielding promising results when compared to the literature. |
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2022-06-20T18:43:30Z2022-06-20T18:43:30Z2021-08-30http://repositorio.ufsm.br/handle/1/24905In the contemporary world, the growing global demand for energy, the reduction of non-renewable energy resources and the concern with the quality of the environment are noticeable. It is well known that the demand for hydrocarbons, which are the most energy source used today, exceeds the reserves forecast for the next decades. Furthermore, the environmental impact that their utilization has caused is responsible for global warming, destruction of the ozone layer, and disappearance of ecological systems in what some people call the sixth massive extinction. Thus, Bio-hydrogen (Bio-H2) can facilitate the decarbonization of certain carbon-emitting activities, as it is produced via dark fermentation, which is considered more viable, ecological and sustainable since it does not require an external energy source. Several types of lignocellulosic biomass can be used as a carbon source by microorganisms producing hydrogen (H2). In this sense, the objective of this work is to use rice husk (RH) and evaluate the production of H2 via the dark fermentation process under different types of inoculums. RH was characterized, prepared and submitted to the acid hydrolysis process with different concentrations of sulfuric acid and evaluated was performed based on the content of total sugars (TS) and at various concentrations of inhibitors. The selected hydrolyzate was then used as a substrate in the dark fermentation process, using two different types of sludge, anaerobic sludge granulation (ASG) and septic tank sludge (STS). The dark fermentation tests were carried out in a reactor with a substrate/inoculum ratio of 9 in the middle-temperature range of 35.8°C and neutral initial pH (6.5 - 7) and monitored for a period of 29 ± 1h approximately. The experimental condition that showed the highest total sugar recovery was the acid pretreatment to 1% v/v H2SO4. The recovery of TS in this condition was 201g kg RH-1, and inhibitor concentrations were less than the limit of quantification of 0.005 g L-1 for Furfural and 0.0005 g L-1 for 5-HMF, and the production and maximum yield of Bio-H2 were 5.37mL g RH-1 and 0.179 mL H2 h-1 g RH-1, respectively. Finally, it is concluded that the hydrolysate (1% v/v H2SO4) as substrate and ASG as inoculum exhibited adequate properties for the generation of Bio-H2 via the dark fermentation method, yielding promising results when compared to the literature.No mundo contemporâneo, é perceptível a crescente demanda global por energia, a redução de recursos energéticos não renováveis e a preocupação com a qualidade do meio ambiente. É sabido que a demanda por hidrocarbonetos, que é a fonte de energia mais utilizada hoje, supera as reservas previstas para as próximas décadas. Além disso, o impacto ambiental que sua utilização causou é responsável pelo aquecimento global, destruição da camada de ozônio e desaparecimento dos sistemas ecológicos no que algumas pessoas chamam de sexta extinção em massa. Assim, o Bio-hidrogênio (Bio-H2) pode facilitar a descarbonização de certas atividades emissoras de carbono, pois produzido via processo de fermentação escura, é considerado mais viável, ecológico e sustentável, uma vez que não necessita de fonte de energia externa e diversos tipos de biomassas lignocelulósicas podem ser utilizadas como fonte de carbono pelos microrganismos produtoras de hidrogênio (H2). Neste sentido, o objetivo deste trabalho é aproveitar a casca de arroz (CA) e avaliar a produção de Bio-H2 via processo de fermentação escura sob diferentes tipos de inóculo. A CA foi caracterizada, preparada e submetida ao processo de hidrólise ácida com diferentes concentrações de H2SO4 e avaliada a partir do teor de açúcares totais (AT) e concentração dos inibidores. O hidrolisado selecionado foi, então, utilizado como substrato no processo de fermentação escura, empregando-se dois diferentes tipos de lodo, o lodo anaeróbico granulado (LAG) e lodo do tanque séptico (LTS), como inóculo. Os ensaios de fermentação escura foram conduzidos no reator com razão substrato/inóculo de 9 na de temperatura meia de 35,8 °C e do pH inicial neutro (6,5 - 7) e monitorados por um período de 29 ± 1h aproximadamente. A condição experimental que apresentou maior recuperação de açúcares totais foi o pré-tratamento ácido ao 1% v/v H2SO4. Nessa condição, a recuperação de AT foi 201g kg CA-1 e as concentrações dos inibidores foram menores do limite de quantificação de 0,005 g L-1 para o furfural e 0,0005 g L-1 para o 5-HMF. Para a produção e rendimento máximo do Bio-H2 foi de 5,37 mL g CA-1 e 0,179 mL H2 h-1 g CA-1, respetivamente. Por fim, conclui-se que o hidrolisado (1% v/v H2SO4) como substrato e LAG como inóculo apresentou características apropriadas para produção de Bio-H2 via processo de fermentação escura, levando a resultados promissores quanto comparados à literatura.porUniversidade Federal de Santa MariaCentro de TecnologiaPrograma de Pós-Graduação em Engenharia AmbientalUFSMBrasilEngenharia AmbientalAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessBiomassa lignocelulósicasHidrólise ácidaBio-HidrogênioLignocellulosic biomassAcid hydrolysisBio-HydrogenCNPQ::ENGENHARIASProdução de bio-hidrogênio a partir da casca de arroz via processo de fermentação escuraProduction of bio-hydrogen from rice husk via dark fermentation processinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisGonzález, Víctor Alcarazhttp://lattes.cnpq.br/2526909747799000Silveira, Andressa de OliveiraCarissimi, ElvisSanta, Ana Lucia PrietoxxxxxxxxxxxxRoman, Lizet Miriam Guevara300000000009600600600600492b98b0-3c96-4151-b8ab-c611cd54a057e3932aad-a9f3-4c5c-aff7-984182201e791ce0ebc6-2a3f-4e9a-9b59-35490b8212507de57ef3-403a-41e8-8638-d72ec944eac7909be645-077c-4267-bafb-0887e3976b08reponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMLICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/24905/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53ORIGINALDIS_PPGEA_2021_ROMAN_LIZET.pdfDIS_PPGEA_2021_ROMAN_LIZET.pdfDissertação de mestradoapplication/pdf1492027http://repositorio.ufsm.br/bitstream/1/24905/1/DIS_PPGEA_2021_ROMAN_LIZET.pdffa7fdf6a20f3e3779a1ca60f99fcd233MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/24905/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD521/249052022-06-20 15:43:30.568oai:repositorio.ufsm.br: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 Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-06-20T18:43:30Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Produção de bio-hidrogênio a partir da casca de arroz via processo de fermentação escura |
dc.title.alternative.eng.fl_str_mv |
Production of bio-hydrogen from rice husk via dark fermentation process |
title |
Produção de bio-hidrogênio a partir da casca de arroz via processo de fermentação escura |
spellingShingle |
Produção de bio-hidrogênio a partir da casca de arroz via processo de fermentação escura Roman, Lizet Miriam Guevara Biomassa lignocelulósicas Hidrólise ácida Bio-Hidrogênio Lignocellulosic biomass Acid hydrolysis Bio-Hydrogen CNPQ::ENGENHARIAS |
title_short |
Produção de bio-hidrogênio a partir da casca de arroz via processo de fermentação escura |
title_full |
Produção de bio-hidrogênio a partir da casca de arroz via processo de fermentação escura |
title_fullStr |
Produção de bio-hidrogênio a partir da casca de arroz via processo de fermentação escura |
title_full_unstemmed |
Produção de bio-hidrogênio a partir da casca de arroz via processo de fermentação escura |
title_sort |
Produção de bio-hidrogênio a partir da casca de arroz via processo de fermentação escura |
author |
Roman, Lizet Miriam Guevara |
author_facet |
Roman, Lizet Miriam Guevara |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
González, Víctor Alcaraz |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2526909747799000 |
dc.contributor.advisor-co1.fl_str_mv |
Silveira, Andressa de Oliveira |
dc.contributor.referee1.fl_str_mv |
Carissimi, Elvis |
dc.contributor.referee2.fl_str_mv |
Santa, Ana Lucia Prieto |
dc.contributor.authorLattes.fl_str_mv |
xxxxxxxxxxxx |
dc.contributor.author.fl_str_mv |
Roman, Lizet Miriam Guevara |
contributor_str_mv |
González, Víctor Alcaraz Silveira, Andressa de Oliveira Carissimi, Elvis Santa, Ana Lucia Prieto |
dc.subject.por.fl_str_mv |
Biomassa lignocelulósicas Hidrólise ácida Bio-Hidrogênio |
topic |
Biomassa lignocelulósicas Hidrólise ácida Bio-Hidrogênio Lignocellulosic biomass Acid hydrolysis Bio-Hydrogen CNPQ::ENGENHARIAS |
dc.subject.eng.fl_str_mv |
Lignocellulosic biomass Acid hydrolysis Bio-Hydrogen |
dc.subject.cnpq.fl_str_mv |
CNPQ::ENGENHARIAS |
description |
In the contemporary world, the growing global demand for energy, the reduction of non-renewable energy resources and the concern with the quality of the environment are noticeable. It is well known that the demand for hydrocarbons, which are the most energy source used today, exceeds the reserves forecast for the next decades. Furthermore, the environmental impact that their utilization has caused is responsible for global warming, destruction of the ozone layer, and disappearance of ecological systems in what some people call the sixth massive extinction. Thus, Bio-hydrogen (Bio-H2) can facilitate the decarbonization of certain carbon-emitting activities, as it is produced via dark fermentation, which is considered more viable, ecological and sustainable since it does not require an external energy source. Several types of lignocellulosic biomass can be used as a carbon source by microorganisms producing hydrogen (H2). In this sense, the objective of this work is to use rice husk (RH) and evaluate the production of H2 via the dark fermentation process under different types of inoculums. RH was characterized, prepared and submitted to the acid hydrolysis process with different concentrations of sulfuric acid and evaluated was performed based on the content of total sugars (TS) and at various concentrations of inhibitors. The selected hydrolyzate was then used as a substrate in the dark fermentation process, using two different types of sludge, anaerobic sludge granulation (ASG) and septic tank sludge (STS). The dark fermentation tests were carried out in a reactor with a substrate/inoculum ratio of 9 in the middle-temperature range of 35.8°C and neutral initial pH (6.5 - 7) and monitored for a period of 29 ± 1h approximately. The experimental condition that showed the highest total sugar recovery was the acid pretreatment to 1% v/v H2SO4. The recovery of TS in this condition was 201g kg RH-1, and inhibitor concentrations were less than the limit of quantification of 0.005 g L-1 for Furfural and 0.0005 g L-1 for 5-HMF, and the production and maximum yield of Bio-H2 were 5.37mL g RH-1 and 0.179 mL H2 h-1 g RH-1, respectively. Finally, it is concluded that the hydrolysate (1% v/v H2SO4) as substrate and ASG as inoculum exhibited adequate properties for the generation of Bio-H2 via the dark fermentation method, yielding promising results when compared to the literature. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021-08-30 |
dc.date.accessioned.fl_str_mv |
2022-06-20T18:43:30Z |
dc.date.available.fl_str_mv |
2022-06-20T18:43:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/24905 |
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http://repositorio.ufsm.br/handle/1/24905 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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300000000009 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
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Universidade Federal de Santa Maria Centro de Tecnologia |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Engenharia Ambiental |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Engenharia Ambiental |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Tecnologia |
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