Determinação multirresíduo de agrotóxicos em banana empregando método QuEChERS modificado e UHPLC-MS/MS

Detalhes bibliográficos
Ano de defesa: 2022
Autor(a) principal: Prochnow, Ana Paula Fernandes lattes
Orientador(a): Zanella, Renato lattes
Banca de defesa: Kemmerich, Magali, Dallago, Rogério Marcos
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
Programa de Pós-Graduação: Programa de Pós-Graduação em Química
Departamento: Química
País: Brasil
Palavras-chave em Português:
Banana
Agrotóxicos
QuEChERS
UHPLC-MS/MS
Palavras-chave em Inglês:
Pesticides
Área do conhecimento CNPq:
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
Link de acesso: http://repositorio.ufsm.br/handle/1/25999
Resumo: In recent years, it has been possible to observe that the use of pesticides in various cultures has increased alarmingly and, in most cases, is added to an inappropriate practice of use, as well as impr oper disposal. Banana, one of the most produced and consumed fruits in the world, is no exception, making the fruit a target for the presence of these residues. Therefore, the present work aimed to develop a fast and efficient sample preparation method for the determination of pesticide residues in bananas using ultrahigh performance liquid chromatography coupled to serial mass spectrometry (UHPLCMS/MS). Initially, the three variations of the QuEChERS method for extracting/partitioning the analytes were t ested. After choosing the QuEChERS citrate method, several sorbents were tested to clean the extract by extraction in a dispersive solid phase. The Oasis HLB ® sorbent presented the highest efficiency of results, the established amount of the sorbent and ma gnesium sulfate for the cleaning step was chosen through a test of proportion comparison between the two. The proposed method was validated for the determination of 82 pesticides for the banana matrix, which showed recoveries of 70 to 118%, RSD ≤20% and of quantification between 5 and 2 5 µg kg1 . The UHPLCme thod limits MS/MS technique provided high selectivity and sensitivity, and sample preparation using modified QuEChERS is fast and efficient. The method was successfully applied to the analysis of banana samples and four pesticides were found, including pesticides not allowed in Brazil for bananas.
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spelling 2022-08-26T12:45:27Z2022-08-26T12:45:27Z2022-04-29http://repositorio.ufsm.br/handle/1/25999In recent years, it has been possible to observe that the use of pesticides in various cultures has increased alarmingly and, in most cases, is added to an inappropriate practice of use, as well as impr oper disposal. Banana, one of the most produced and consumed fruits in the world, is no exception, making the fruit a target for the presence of these residues. Therefore, the present work aimed to develop a fast and efficient sample preparation method for the determination of pesticide residues in bananas using ultrahigh performance liquid chromatography coupled to serial mass spectrometry (UHPLCMS/MS). Initially, the three variations of the QuEChERS method for extracting/partitioning the analytes were t ested. After choosing the QuEChERS citrate method, several sorbents were tested to clean the extract by extraction in a dispersive solid phase. The Oasis HLB ® sorbent presented the highest efficiency of results, the established amount of the sorbent and ma gnesium sulfate for the cleaning step was chosen through a test of proportion comparison between the two. The proposed method was validated for the determination of 82 pesticides for the banana matrix, which showed recoveries of 70 to 118%, RSD ≤20% and of quantification between 5 and 2 5 µg kg1 . The UHPLCme thod limits MS/MS technique provided high selectivity and sensitivity, and sample preparation using modified QuEChERS is fast and efficient. The method was successfully applied to the analysis of banana samples and four pesticides were found, including pesticides not allowed in Brazil for bananas.Nos últimos anos foi possível observar que o uso de agrotóxicos em diversas culturas aumentou de forma alarmante e, na maioria das vezes, vem somado a uma prática inadequada de uso, bem como descartes indevidos. A banana, uma das frutas mais produzidas e consumidas no mundo, não é exceção, tornando a fruta um alvo para a presença desses resíduos. Sendo assim, o presente trabalho teve como objetivo desenvolver um método de preparo de amostra rápido e eficiente para a determinação de resíduos de agrotóxicos em banana empregando cromatografia líquida de ultraalta eficiência acoplada à espectrometria de massas em série (UHPLC-MS/MS). Inicialmente foram testadas as três variações do método QuEChERS para extração/partição dos analitos. Após a escolha do método QuEChERS citrato foram testados vários sorventes para limpeza do extrato por extração em fase sólida dispersiva. O sorvente Oasis HLB® apresentou a maior eficiência de resultados, a quantidade estabelecida do sorvente e sulfato de magnésio para a etapa de limpeza foi escolhida através de experimento de comparação de proporção entre os dois. O método proposto foi validado para a determinação de 82 agrotóxicos para a matriz banana, os quais apresentaram recuperações de 70 a 118%, RSD ≤20% e limites de quantificação do método entre 5 e 25 µg kg-1. A técnica de UHPLC-MS/MS proporcionou alta seletividade e sensibilidade, e o preparo de amostra empregando QuEChERS modificado é rápido e eficiente. O método foi aplicado com sucesso para a análise de amostras de banana e quatro pesticidas foram encontrados, incluindo agrotóxicos não permitidos no Brasil para banana.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em QuímicaUFSMBrasilQuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessBananaAgrotóxicosQuEChERSUHPLC-MS/MSPesticidesCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICADeterminação multirresíduo de agrotóxicos em banana empregando método QuEChERS modificado e UHPLC-MS/MSMultireside determination of pesticides in banana using modified QuEChERS method and UHPLCMS/MSinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisZanella, Renatohttp://lattes.cnpq.br/2541865299438479Adaime , Martha BohrerKemmerich, MagaliDallago, Rogério Marcoshttp://lattes.cnpq.br/3015806996764116Prochnow, Ana Paula Fernandes1006000000006006006006006006000aa2baab-94e8-4d3d-bbb2-85495ba77db546ab594f-88f8-4c95-a1d5-8263a3142ac8e3f47b1c-bced-4ba5-a0c9-4d0204119f8527c1a555-cd54-4899-9c4c-b409ab942ea89050c1fd-3eda-454b-bdf7-f23a3ce1cd7freponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMLICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/25999/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53ORIGINALDIS_PPGQUÍMICA_2022_PROCHNOW_ANA_PAULA.pdfDIS_PPGQUÍMICA_2022_PROCHNOW_ANA_PAULA.pdfDissertação de mestradoapplication/pdf1908340http://repositorio.ufsm.br/bitstream/1/25999/1/DIS_PPGQU%c3%8dMICA_2022_PROCHNOW_ANA_PAULA.pdfe4c6c9b71899e05e293bc07a30a5f4a6MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/25999/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD521/259992022-08-26 09:45:27.65oai:repositorio.ufsm.br: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 Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-08-26T12:45:27Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.por.fl_str_mv Determinação multirresíduo de agrotóxicos em banana empregando método QuEChERS modificado e UHPLC-MS/MS
dc.title.alternative.eng.fl_str_mv Multireside determination of pesticides in banana using modified QuEChERS method and UHPLCMS/MS
title Determinação multirresíduo de agrotóxicos em banana empregando método QuEChERS modificado e UHPLC-MS/MS
spellingShingle Determinação multirresíduo de agrotóxicos em banana empregando método QuEChERS modificado e UHPLC-MS/MS
Prochnow, Ana Paula Fernandes
Banana
Agrotóxicos
QuEChERS
UHPLC-MS/MS
Pesticides
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
title_short Determinação multirresíduo de agrotóxicos em banana empregando método QuEChERS modificado e UHPLC-MS/MS
title_full Determinação multirresíduo de agrotóxicos em banana empregando método QuEChERS modificado e UHPLC-MS/MS
title_fullStr Determinação multirresíduo de agrotóxicos em banana empregando método QuEChERS modificado e UHPLC-MS/MS
title_full_unstemmed Determinação multirresíduo de agrotóxicos em banana empregando método QuEChERS modificado e UHPLC-MS/MS
title_sort Determinação multirresíduo de agrotóxicos em banana empregando método QuEChERS modificado e UHPLC-MS/MS
author Prochnow, Ana Paula Fernandes
author_facet Prochnow, Ana Paula Fernandes
author_role author
dc.contributor.advisor1.fl_str_mv Zanella, Renato
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/2541865299438479
dc.contributor.advisor-co1.fl_str_mv Adaime , Martha Bohrer
dc.contributor.referee1.fl_str_mv Kemmerich, Magali
dc.contributor.referee2.fl_str_mv Dallago, Rogério Marcos
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3015806996764116
dc.contributor.author.fl_str_mv Prochnow, Ana Paula Fernandes
contributor_str_mv Zanella, Renato
Adaime , Martha Bohrer
Kemmerich, Magali
Dallago, Rogério Marcos
dc.subject.por.fl_str_mv Banana
Agrotóxicos
QuEChERS
UHPLC-MS/MS
topic Banana
Agrotóxicos
QuEChERS
UHPLC-MS/MS
Pesticides
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
dc.subject.eng.fl_str_mv Pesticides
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
description In recent years, it has been possible to observe that the use of pesticides in various cultures has increased alarmingly and, in most cases, is added to an inappropriate practice of use, as well as impr oper disposal. Banana, one of the most produced and consumed fruits in the world, is no exception, making the fruit a target for the presence of these residues. Therefore, the present work aimed to develop a fast and efficient sample preparation method for the determination of pesticide residues in bananas using ultrahigh performance liquid chromatography coupled to serial mass spectrometry (UHPLCMS/MS). Initially, the three variations of the QuEChERS method for extracting/partitioning the analytes were t ested. After choosing the QuEChERS citrate method, several sorbents were tested to clean the extract by extraction in a dispersive solid phase. The Oasis HLB ® sorbent presented the highest efficiency of results, the established amount of the sorbent and ma gnesium sulfate for the cleaning step was chosen through a test of proportion comparison between the two. The proposed method was validated for the determination of 82 pesticides for the banana matrix, which showed recoveries of 70 to 118%, RSD ≤20% and of quantification between 5 and 2 5 µg kg1 . The UHPLCme thod limits MS/MS technique provided high selectivity and sensitivity, and sample preparation using modified QuEChERS is fast and efficient. The method was successfully applied to the analysis of banana samples and four pesticides were found, including pesticides not allowed in Brazil for bananas.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-08-26T12:45:27Z
dc.date.available.fl_str_mv 2022-08-26T12:45:27Z
dc.date.issued.fl_str_mv 2022-04-29
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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url http://repositorio.ufsm.br/handle/1/25999
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dc.relation.cnpq.fl_str_mv 100600000000
dc.relation.confidence.fl_str_mv 600
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dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
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rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Química
dc.publisher.initials.fl_str_mv UFSM
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Química
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
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