O potencial farmacológico e antimicrobiano do fruto Tucumã (Astrocaryum aculeatum)

• Ano de defesa: 2013
• Autor(a) principal: Jobim, Micheli Lamberti
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• dARK ID: ark:/26339/001300000cm46
• Idioma: por
• Instituição de defesa: Universidade Federal de Santa Maria; BR; Farmacologia; UFSM; Programa de Pós-Graduação em Farmacologia
• Palavras-chave em Português: Astrocaryum aculeatum; Gram- positivas; Candida albicans; Estresse oxidativo; Polifenóis; Gram-positive; Oxidative stress; Polyphenols; CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
• Link de acesso: http://repositorio.ufsm.br/handle/1/8994

Several compound present in fruits as polyphenols are able to kill or inhibit the growth of microorganisms. These proprieties are relevant mainly in tropical areas, as Amazonian region where infectious are highly prevalent. Therefore, this study investigated the tucumã Amazonian fruit antimicrobial activity against 37 microorganisms. The potential role of oxidative metabolism imbalance was also studied as causal mechanism of antimicrobial activity. The results showed antibacterial effect of pulp and peel tucumã hydro-alcoholic extracts on three gram-positive bacteria (Enteroccocus faecalis, Bacillus cereus, Listeria monocytogenes) and antifungical effect against Candida albicans. The antimicrobial contribution of main chemical compounds (quercetin, rutin, β-carotene and gallic, caffeic and chlorogenic acids) found in tucumã extracts was also investigated showing an inhibitory effect depending of the organism mainly by quercetin in bacteria and rutin in C.albicans. Analysis of kinetic of DNA releasing in extracellular medium by fluorescence using DNA Pico Green assay® and reactive oxygen species production (ROS) showed potential oxidative imbalance contribution on tucumã inhibitory effect. In B.cereus and C.albicans this effect was clear since after 24 hours the ROS levels were higher when compared to negative control group. In conclusion, tucumã extracts present antimicrobial activity to four microorganisms that have large problems of drug resistance, and the possible mechanism of action of this Amazon fruit is related to REDOX imbalance.