Determinação de farmacos em efluentes hospitalares utilizando UHPLC-QTOF MS, UHPLC-MS/MS e avaliação dos quocientes de risco ambiental
Ano de defesa: | 2023 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Química
|
Departamento: |
Química
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/30518 |
Resumo: | One of the main sources of pollution of aquatic systems by drugs is the incorrect disposal of hospital effluents (HWW). The HWW matrix is quite complex and therefore its analysis is a challenge, in this context, sample preparation plays an important role in the determination of these substances. The combination of DSPE and SPE techniques was evaluated in the preparation stage of HWW samples, from two hospitals (HA and HB) in Porto Alegre (RS, Brazil), and the use of a method of direct injection of samples from a hospital (HC) in Santa Maria (RS, Brazil), for the determination of pharmaceuticals and metabolites and subsequent study of environmental risk. The combined method presented in this study had was successfully employed for the screening and quantification of pharmacological compounds and metabolites present in hospital effluents, with heterogeneous complexity characteristics. The screening method enabled the identification of five metabolites and 45 active principles, from which 24 were classified as suspect and 22 confirmed with standards. The quantification method had a linear response for 35 compounds and it was possible to quantify 22 analytes present in the samples, in a range from 1.17 µg L-1 to 239.64 µg L-1 . Furthermore, the direct injection analysis method presented in this study was successfully employed for the quantification of 35 compounds, of which up to 7 presented values above the LOQ of the method in hospital effluent. The HC samples showed concentrations in a range of 3.02 µg L-1 to 235.18 µg L-1 . There are indications of climatic conditions influence regarding to the concentration of drugs present in the effluent. The environmental risk assessment provided an RQ value > 1 for several compounds quantified in the HA and HB samples, with RQmix in the order of 30x106 for HA and 20x106 for HB. The RQmix for the samples from HC were in the order of 8x104 and 2x105 , respectively on winter and summer. The effluents from the three evaluated hospitals present an environmental risk for aquatic species and small rodents, based on the results of RQ and RQmix, and contribute to the fate and persistence of drugs in the environment. |
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2023-11-16T19:03:57Z2023-11-16T19:03:57Z2023-10-02http://repositorio.ufsm.br/handle/1/30518One of the main sources of pollution of aquatic systems by drugs is the incorrect disposal of hospital effluents (HWW). The HWW matrix is quite complex and therefore its analysis is a challenge, in this context, sample preparation plays an important role in the determination of these substances. The combination of DSPE and SPE techniques was evaluated in the preparation stage of HWW samples, from two hospitals (HA and HB) in Porto Alegre (RS, Brazil), and the use of a method of direct injection of samples from a hospital (HC) in Santa Maria (RS, Brazil), for the determination of pharmaceuticals and metabolites and subsequent study of environmental risk. The combined method presented in this study had was successfully employed for the screening and quantification of pharmacological compounds and metabolites present in hospital effluents, with heterogeneous complexity characteristics. The screening method enabled the identification of five metabolites and 45 active principles, from which 24 were classified as suspect and 22 confirmed with standards. The quantification method had a linear response for 35 compounds and it was possible to quantify 22 analytes present in the samples, in a range from 1.17 µg L-1 to 239.64 µg L-1 . Furthermore, the direct injection analysis method presented in this study was successfully employed for the quantification of 35 compounds, of which up to 7 presented values above the LOQ of the method in hospital effluent. The HC samples showed concentrations in a range of 3.02 µg L-1 to 235.18 µg L-1 . There are indications of climatic conditions influence regarding to the concentration of drugs present in the effluent. The environmental risk assessment provided an RQ value > 1 for several compounds quantified in the HA and HB samples, with RQmix in the order of 30x106 for HA and 20x106 for HB. The RQmix for the samples from HC were in the order of 8x104 and 2x105 , respectively on winter and summer. The effluents from the three evaluated hospitals present an environmental risk for aquatic species and small rodents, based on the results of RQ and RQmix, and contribute to the fate and persistence of drugs in the environment.Uma das principais fontes de poluição dos sistemas aquáticos por fármacos é a disposição incorreta de efluentes hospitalares (HWW). A matriz HWW é bastante complexa e desse modo a sua análise é um desafio e, neste contexto, o preparo de amostra tem um papel importante na determinação destas substâncias. Avaliou-se a combinação das técnicas de DSPE e SPE na etapa de preparo de amostra de HWW, provenientes de dois hospitais (HA e HB) de Porto Alegre (RS, Brasil). Além disso, utilizou-se um método de injeção direta de amostra de um hospital (HC) de Santa Maria (RS, Brasil), para a determinação de fármacos e metabólitos e posterior estudo de risco ambiental. O método de preparo de amostra combinado apresentado neste estudo teve bons resultados para o screening e a quantificação de fármacos e metabólitos presentes em efluentes hospitalares, com características de complexidade bastante heterogênea. O método de screening, empregando UHPLC-QTOF, possibilitou a identificação de cinco metabólitos e 46 princípios ativos, dos quais 24 foram classificados como suspeitos e 22 confirmados com padrões. O método de quantificação por UHPLCMS/MS teve resposta linear para 35 compostos e foi possível quantificar 22 analitos presentes nas amostras, em uma faixa de 1,17 µgL-1 a 239,64 µgL-1 . Ademais, o método de análise com injeção direta apresentado neste estudo teve bons resultados para a quantificação de 35 compostos, dos quais 7 apresentaram valores a cima do LOQ do método em efluente hospitalar. As amostras do HC apresentaram concentrações em uma faixa de 3,02 µgL-1 a 235,18 µgL-1 . Há indícios da influência das condições climáticas no que diz respeito à concentração dos fármacos presentes no efluente. A avaliação de risco ambiental aprestou valor de RQ>1 para vários compostos quantificados nas amostras de HA e HB, com RQmix na ordem de 30x106 para HA e 20x106 para HB. Os RQmix das amostras do HC ficaram na ordem de 8x104 e 2x105 para inverno e verão, respectivamente. Observa-se que os efluentes dos três hospitais avaliados apresentam risco ambiental para espécies aquáticas e pequenos roedores, baseado nos resultados de RQ e RQmix, e contribuem para o destino e persistência de fármacos no meio ambiente.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em QuímicaUFSMBrasilQuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessEfluente hospitalarFármacosAnálise qualitativaAnálise quantitativaAnálise de risco ambientalHospital wastewaterPharmaceuticalsQuantitative analysisQualitative analysisEnvironmental risk assessmentCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICADeterminação de farmacos em efluentes hospitalares utilizando UHPLC-QTOF MS, UHPLC-MS/MS e avaliação dos quocientes de risco ambientalDetermination of pharmaceuticals in hospital wastewaters using UHPLC-QTOF MS, UHPLC-MS/MS and evaluation of environmentl risk quotientinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisPrestes, Osmar Damianhttp://lattes.cnpq.br/9632234955509028Sirtori, CarlaAmaral, Camila Costa de AmorimSouza, Darliana MelloGonçalves, Fábio FerreiraBecker, Raquel Wielenshttp://lattes.cnpq.br/0529456264431690Mühlen, Lisandro von100600000000600600600600600600600600f79cc0c9-c923-4244-9c1f-c80d74674410a3b59dbe-5d47-489c-85e1-3bc2c52604d8fe6d02e7-7dd9-4498-8565-b6bb95ce46b86a27ed78-920e-4cfc-8760-b99c8c79c285d33ad996-109a-4356-a60d-468ae8c0b65be18ecce1-8501-4394-8179-133603c19355c3a4ec38-8209-4528-bd91-b4f93e2519bbreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALTES_PPGQUIMICA_2023_MUHLEN_LISANDRO.pdfTES_PPGQUIMICA_2023_MUHLEN_LISANDRO.pdfTese de doutoradoapplication/pdf2587995http://repositorio.ufsm.br/bitstream/1/30518/1/TES_PPGQUIMICA_2023_MUHLEN_LISANDRO.pdf6d88ce395a1adcf36f2cf33213e2fc32MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Determinação de farmacos em efluentes hospitalares utilizando UHPLC-QTOF MS, UHPLC-MS/MS e avaliação dos quocientes de risco ambiental |
dc.title.alternative.eng.fl_str_mv |
Determination of pharmaceuticals in hospital wastewaters using UHPLC-QTOF MS, UHPLC-MS/MS and evaluation of environmentl risk quotient |
title |
Determinação de farmacos em efluentes hospitalares utilizando UHPLC-QTOF MS, UHPLC-MS/MS e avaliação dos quocientes de risco ambiental |
spellingShingle |
Determinação de farmacos em efluentes hospitalares utilizando UHPLC-QTOF MS, UHPLC-MS/MS e avaliação dos quocientes de risco ambiental Mühlen, Lisandro von Efluente hospitalar Fármacos Análise qualitativa Análise quantitativa Análise de risco ambiental Hospital wastewater Pharmaceuticals Quantitative analysis Qualitative analysis Environmental risk assessment CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Determinação de farmacos em efluentes hospitalares utilizando UHPLC-QTOF MS, UHPLC-MS/MS e avaliação dos quocientes de risco ambiental |
title_full |
Determinação de farmacos em efluentes hospitalares utilizando UHPLC-QTOF MS, UHPLC-MS/MS e avaliação dos quocientes de risco ambiental |
title_fullStr |
Determinação de farmacos em efluentes hospitalares utilizando UHPLC-QTOF MS, UHPLC-MS/MS e avaliação dos quocientes de risco ambiental |
title_full_unstemmed |
Determinação de farmacos em efluentes hospitalares utilizando UHPLC-QTOF MS, UHPLC-MS/MS e avaliação dos quocientes de risco ambiental |
title_sort |
Determinação de farmacos em efluentes hospitalares utilizando UHPLC-QTOF MS, UHPLC-MS/MS e avaliação dos quocientes de risco ambiental |
author |
Mühlen, Lisandro von |
author_facet |
Mühlen, Lisandro von |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Prestes, Osmar Damian |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9632234955509028 |
dc.contributor.advisor-co1.fl_str_mv |
Sirtori, Carla |
dc.contributor.referee1.fl_str_mv |
Amaral, Camila Costa de Amorim |
dc.contributor.referee2.fl_str_mv |
Souza, Darliana Mello |
dc.contributor.referee3.fl_str_mv |
Gonçalves, Fábio Ferreira |
dc.contributor.referee4.fl_str_mv |
Becker, Raquel Wielens |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0529456264431690 |
dc.contributor.author.fl_str_mv |
Mühlen, Lisandro von |
contributor_str_mv |
Prestes, Osmar Damian Sirtori, Carla Amaral, Camila Costa de Amorim Souza, Darliana Mello Gonçalves, Fábio Ferreira Becker, Raquel Wielens |
dc.subject.por.fl_str_mv |
Efluente hospitalar Fármacos Análise qualitativa Análise quantitativa Análise de risco ambiental |
topic |
Efluente hospitalar Fármacos Análise qualitativa Análise quantitativa Análise de risco ambiental Hospital wastewater Pharmaceuticals Quantitative analysis Qualitative analysis Environmental risk assessment CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Hospital wastewater Pharmaceuticals Quantitative analysis Qualitative analysis Environmental risk assessment |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
One of the main sources of pollution of aquatic systems by drugs is the incorrect disposal of hospital effluents (HWW). The HWW matrix is quite complex and therefore its analysis is a challenge, in this context, sample preparation plays an important role in the determination of these substances. The combination of DSPE and SPE techniques was evaluated in the preparation stage of HWW samples, from two hospitals (HA and HB) in Porto Alegre (RS, Brazil), and the use of a method of direct injection of samples from a hospital (HC) in Santa Maria (RS, Brazil), for the determination of pharmaceuticals and metabolites and subsequent study of environmental risk. The combined method presented in this study had was successfully employed for the screening and quantification of pharmacological compounds and metabolites present in hospital effluents, with heterogeneous complexity characteristics. The screening method enabled the identification of five metabolites and 45 active principles, from which 24 were classified as suspect and 22 confirmed with standards. The quantification method had a linear response for 35 compounds and it was possible to quantify 22 analytes present in the samples, in a range from 1.17 µg L-1 to 239.64 µg L-1 . Furthermore, the direct injection analysis method presented in this study was successfully employed for the quantification of 35 compounds, of which up to 7 presented values above the LOQ of the method in hospital effluent. The HC samples showed concentrations in a range of 3.02 µg L-1 to 235.18 µg L-1 . There are indications of climatic conditions influence regarding to the concentration of drugs present in the effluent. The environmental risk assessment provided an RQ value > 1 for several compounds quantified in the HA and HB samples, with RQmix in the order of 30x106 for HA and 20x106 for HB. The RQmix for the samples from HC were in the order of 8x104 and 2x105 , respectively on winter and summer. The effluents from the three evaluated hospitals present an environmental risk for aquatic species and small rodents, based on the results of RQ and RQmix, and contribute to the fate and persistence of drugs in the environment. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-11-16T19:03:57Z |
dc.date.available.fl_str_mv |
2023-11-16T19:03:57Z |
dc.date.issued.fl_str_mv |
2023-10-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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http://repositorio.ufsm.br/handle/1/30518 |
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http://repositorio.ufsm.br/handle/1/30518 |
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por |
language |
por |
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100600000000 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Química |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Química |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
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Biblioteca Digital de Teses e Dissertações do UFSM |
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