Parâmetros toxicológicos em carpas (Cyprinus carpio) expostos a uma formulação comercial de clomazone (gamit®)
Ano de defesa: | 2011 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
|
Departamento: |
Bioquímica
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/4435 |
Resumo: | Commercial formulations of the herbicide clomazone have been widely used in agriculture and fish farming to control weeds. Fish can be affected when the water reaches the drainage waterways, causing an imbalance in the aquatic ecosystem. In order to evaluate a possible contamination, we determined LC50 (96h) using a commercial formulation containing clomazone (Gamit®). We verified the metabolic, enzymatic, and genotoxic parameters as well as the oxidative stress in juvenile carp (Cyprinus carpio). Firstly, in order to determine the LC50 for the acute toxicity test, , the animals were exposed to the concentrations of 10, 20, 30, 40 and 50 mg/L of clomazone for 96 h and the fish behavior was analyzed during this period. After exposure, we verified the activity of the enzyme acetylcholinesterase (AChE) in muscle and brain of the dead fish and the live carp. Secondly, fish were exposed to the herbicide for 7 days, both in field (rice crop) and laboratory conditions. The concentration used in the rice crop and the laboratory was 0.5 mg/L. After the experimental period of 7 days under laboratory conditions and 7, 30, and 90 days under rice crop conditions, brain, liver and muscle of fish were sampled to carry out the toxicological analysis. The parameters studied were enzymatic activity of AChE, catalase (CAT) and glutathione S-tranferase (GST) in different tissues of this species. Some markers of oxidative stress, such as protein carbonylation and thiobarbituric acid reactive substances (TBARS) levels in liver tissue were also analyzed. Some metabolic parameters such as glucose, glycogen, lactate, protein, and ammonia as well as amino acids in liver and muscle of carp were also measured. In plasma, we measured glucose, lactate, and protein. Thirdly, carp were exposed to approximately 15% of the LC50 (5.0 mg/L) for 7 days. We verified the formation of reactive oxygen species (ROS) and micronucleus, and the comet test was performed in order to investigate the presence of DNA damage. In the first study we observed that fish exposed to 20, 30, 40 and 50 mg/L showed behavioral changes and LC50 (96h) was 30.35 mg/L. In addition, it was found that the activity of the enzyme AChE showed no significant changes in the brain of the fish that died at the concentrations tested (30, 40 and 50 mg/L), and the muscle of dead fish had an increase in this enzyme when they were exposed to 50 mg/L of clomazone. The activity decreased significantly in the brain AChE of fish that remained alive after 96 h of exposure to 10, 20 and 30 mg/L, but increased in the muscle of the surviving fish exposed to all concentrations tested. In the second experiment results showed that fish exposed to 7 days had no changes in AChE under field conditions. However, a decrease in the activity of this enzyme in muscle was observed under laboratory conditions. During the same period of exposition, the parameters of oxidative stress conditions changed both under the field and in laboratory conditions. However, metabolic parameters were altered only under field conditions. After 30 and 90 days, AChE activity did not change under field conditions. Disturbances in oxidative stress parameters and metabolism in different tissues were evident in the tissues up to 90 days after treatment. The results showed that the activity of AChE changed only under laboratory conditions, and that oxidative stress associated with metabolic parameters can be good indicators of contamination for clomazone in C. Carpio in rice field conditions. In the third experiment, the results showed an increased formation of ROS and a significant micronucleus (MN) and DNA damage in erythrocytes after exposure to 5.0 mg/L of the herbicide clomazone. Given these results, we can conclude that the herbicide studied can be dangerous to Cyprinus carpio when exposed to it due to increased ROS, which in turn cause oxidative stress evidenced by changes in enzyme markers, metabolic, genotoxic and oxidative stress. However, further studies are needed in order to verify the safety of this herbicide is when associating rice and fish. |
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2017-04-262017-04-262011-10-07CATTANEO, Roberta. Toxicological parameters in carp (Cyprinus carpio) exposed to a commercial formulation of clomazone (gamit®). 2011. 146 f. Tese (Doutorado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/4435Commercial formulations of the herbicide clomazone have been widely used in agriculture and fish farming to control weeds. Fish can be affected when the water reaches the drainage waterways, causing an imbalance in the aquatic ecosystem. In order to evaluate a possible contamination, we determined LC50 (96h) using a commercial formulation containing clomazone (Gamit®). We verified the metabolic, enzymatic, and genotoxic parameters as well as the oxidative stress in juvenile carp (Cyprinus carpio). Firstly, in order to determine the LC50 for the acute toxicity test, , the animals were exposed to the concentrations of 10, 20, 30, 40 and 50 mg/L of clomazone for 96 h and the fish behavior was analyzed during this period. After exposure, we verified the activity of the enzyme acetylcholinesterase (AChE) in muscle and brain of the dead fish and the live carp. Secondly, fish were exposed to the herbicide for 7 days, both in field (rice crop) and laboratory conditions. The concentration used in the rice crop and the laboratory was 0.5 mg/L. After the experimental period of 7 days under laboratory conditions and 7, 30, and 90 days under rice crop conditions, brain, liver and muscle of fish were sampled to carry out the toxicological analysis. The parameters studied were enzymatic activity of AChE, catalase (CAT) and glutathione S-tranferase (GST) in different tissues of this species. Some markers of oxidative stress, such as protein carbonylation and thiobarbituric acid reactive substances (TBARS) levels in liver tissue were also analyzed. Some metabolic parameters such as glucose, glycogen, lactate, protein, and ammonia as well as amino acids in liver and muscle of carp were also measured. In plasma, we measured glucose, lactate, and protein. Thirdly, carp were exposed to approximately 15% of the LC50 (5.0 mg/L) for 7 days. We verified the formation of reactive oxygen species (ROS) and micronucleus, and the comet test was performed in order to investigate the presence of DNA damage. In the first study we observed that fish exposed to 20, 30, 40 and 50 mg/L showed behavioral changes and LC50 (96h) was 30.35 mg/L. In addition, it was found that the activity of the enzyme AChE showed no significant changes in the brain of the fish that died at the concentrations tested (30, 40 and 50 mg/L), and the muscle of dead fish had an increase in this enzyme when they were exposed to 50 mg/L of clomazone. The activity decreased significantly in the brain AChE of fish that remained alive after 96 h of exposure to 10, 20 and 30 mg/L, but increased in the muscle of the surviving fish exposed to all concentrations tested. In the second experiment results showed that fish exposed to 7 days had no changes in AChE under field conditions. However, a decrease in the activity of this enzyme in muscle was observed under laboratory conditions. During the same period of exposition, the parameters of oxidative stress conditions changed both under the field and in laboratory conditions. However, metabolic parameters were altered only under field conditions. After 30 and 90 days, AChE activity did not change under field conditions. Disturbances in oxidative stress parameters and metabolism in different tissues were evident in the tissues up to 90 days after treatment. The results showed that the activity of AChE changed only under laboratory conditions, and that oxidative stress associated with metabolic parameters can be good indicators of contamination for clomazone in C. Carpio in rice field conditions. In the third experiment, the results showed an increased formation of ROS and a significant micronucleus (MN) and DNA damage in erythrocytes after exposure to 5.0 mg/L of the herbicide clomazone. Given these results, we can conclude that the herbicide studied can be dangerous to Cyprinus carpio when exposed to it due to increased ROS, which in turn cause oxidative stress evidenced by changes in enzyme markers, metabolic, genotoxic and oxidative stress. However, further studies are needed in order to verify the safety of this herbicide is when associating rice and fish.As formulações comerciais do herbicida clomazone têm sido amplamente utilizadas na agricultura e na piscicultura para controle de plantas daninhas. Os peixes podem ser afetados quando as águas de drenagem atingem os cursos d água, acarretando um desequilíbrio no ecossistema aquático. Para avaliar uma possível contaminação foi determinada a CL50 (96h) utilizando-se uma formulação comercial contendo clomazone (Gamit®) verficando-se parâmetros metabólicos, enzimáticos, genotóxicos e de estresse oxidativo em juvenis de carpas (Cyprinus carpio). Para o teste de toxicidade aguda, a fim de determinar a CL50, os peixes foram expostos às concentrações 10, 20, 30, 40 e 50 mg/L de clomazone em água durante 96 horas e o comportamento dos peixes foi analisado nesse período. Após a exposição, foi verificada a atividade da enzima acetilcolinesterase (AChE) no cérebro e músculo dos peixes mortos e das carpas que sobreviveram. No segundo experimento, os peixes foram expostos ao herbicida durante sete dias, tanto em condição de campo (lavoura de arroz irrigado) como de laboratório. A concentração utilizada na lavoura de arroz e no laboratório foi de 0,5 mg/L. Decorridos os períodos experimentais de 7 dias em condições de laboratório, e 7, 30 e 90 dias em condições de lavoura de arroz, foram retirados o cérebro, o fígado e o músculo dos peixes para realização das análises toxicológicas. Os parâmetros enzimáticos analisados foram a atividade da AChE, catalase (CAT) e glutationa S-transferase (GST). Também foram analisados alguns marcadores de estresse oxidativo, como a carbonilação de proteínas e níveis das substâncias reativas ao ácido tiobarbitúrico (TBARS) no tecido hepático. Por fim, foram avaliados alguns parâmetros metabólicos como: glicose, glicogênio, lactato, proteína, amônia e os aminoácidos em fígado e em músculo de carpas. No plasma, foram feitas as dosagens de glicose, de lactato e de proteína. Em um terceiro experimento, as carpas foram expostas a aproximadamente 15% do valor obtido para a CL50 (5,0mg/L) por 7 dias. Posteriormente, foi verificada a formação das espécies reativas de oxigênio (EROs) e foram realizados os testes do micronúcleo e cometa (expresso por índice de dano do DNA). No primeiro experimento, foi observado que os peixes expostos a 20, 30, 40 e 50 mg/L, mostraram mudanças comportamentais e a CL50 (96h) foi 30,35 mg/L. Além disso, foi verificado que a atividade da enzima AChE não apresentou alterações significativas no cérebro dos peixes que morreram nas concentrações testadas (30, 40 e 50mg/L), e no músculo dos peixes mortos houve uma elevação na atividade desta enzima, quando eles foram expostos a 50 mg/L de clomazone. Já a atividade AChE diminuiu significativamente no cérebro dos peixes que sobreviveram após 96h de exposição a 10, 20 e 30mg/L, no entanto, aumentou no músculo dos peixes sobreviventes expostos a todas as concentrações testadas. No segundo experimento, os resultados mostraram que os peixes expostos a 7 dias não apresentaram alterações na AChE em condições de campo. No entanto, uma diminuição da atividade desta enzima no músculo foi observada em condições de laboratório. Durante o mesmo período de exposição, os parâmetros de estresse oxidativo mudaram tanto em condições de campo quanto em laboratório. Entretanto, os parâmetros metabólicos foram alterados apenas em condições de campo. Após 30 e 90 dias, a atividade da AChE não se alterou em condições de campo. Distúrbios nos parâmetros de estresse oxidativo e metabolismo foram evidentes nos tecidos até 90 dias após a aplicação. Os resultados mostraram que a atividade da AChE alterou apenas em condições de laboratório, e que marcadores de estresse oxidativo associados aos parâmetros metabólicos podem ser bons indicadores de contaminação para o clomazone em C. carpio em condições de campo. No terceiro experimento, os resultados mostraram um aumento da formação das EROs e significativo aumento dos MN e de danos no DNA dos eritrócitos após a exposição a 5,0 mg/L do herbicida clomazone. De acordo com os resultados obtidos, conclui-se que o herbicida estudado pode ser perigoso para as carpas quando expostas, devido ao aumento das EROs que por sua vez causam estresse oxidativo, evidenciado por alterações em marcadores enzimáticos, metabólicos, genotóxicos e de estresse oxidativo. Contudo, mais estudos serão necessários para verificar a segurança desse herbicida para os cultivos associados utilizando-se arroz e peixes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaClomazoneToxicidadeRizipisciculturaCarpasToxicityRice-fish culture carpCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAParâmetros toxicológicos em carpas (Cyprinus carpio) expostos a uma formulação comercial de clomazone (gamit®)Toxicological parameters in carp (Cyprinus carpio) exposed to a commercial formulation of clomazone (gamit®)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisLoro, Vania Luciahttp://lattes.cnpq.br/6392817606416780Moreira, Heden Luiz Marqueshttp://lattes.cnpq.br/1229541551615355Barcellos, Leonardo José Gilhttp://lattes.cnpq.br/4504482634911546Barbosa, Nilda Berenice de Vargashttp://lattes.cnpq.br/5901511067144019Rubin, Maribel Antonellohttp://lattes.cnpq.br/7237734243628134http://lattes.cnpq.br/4282856921907832Cattaneo, Roberta200800000002400300300300500500500b0918185-c6b7-49df-b8d6-d245d73535b85af577fa-f9c5-4b4d-8ce8-39997cfe632f857ed407-0858-41be-8120-d611d61e335fde1fbfb1-090e-4afb-99c2-1e8307678fbc0198eecb-263d-4159-82c5-3698b0253f4f4e00cef5-a143-41b6-823b-85c46190f429info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALCATTANEO, ROBERTA.pdfapplication/pdf1342973http://repositorio.ufsm.br/bitstream/1/4435/1/CATTANEO%2c%20ROBERTA.pdfd4439e1627fa5c8c19122119ad89bb54MD51TEXTCATTANEO, ROBERTA.pdf.txtCATTANEO, ROBERTA.pdf.txtExtracted texttext/plain245622http://repositorio.ufsm.br/bitstream/1/4435/2/CATTANEO%2c%20ROBERTA.pdf.txt8daaaf11c55151160074eef60b851c12MD52THUMBNAILCATTANEO, ROBERTA.pdf.jpgCATTANEO, ROBERTA.pdf.jpgIM Thumbnailimage/jpeg4999http://repositorio.ufsm.br/bitstream/1/4435/3/CATTANEO%2c%20ROBERTA.pdf.jpg9264190a737a676a2273638e045bd099MD531/44352022-09-09 08:56:05.911oai:repositorio.ufsm.br:1/4435Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-09-09T11:56:05Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Parâmetros toxicológicos em carpas (Cyprinus carpio) expostos a uma formulação comercial de clomazone (gamit®) |
dc.title.alternative.eng.fl_str_mv |
Toxicological parameters in carp (Cyprinus carpio) exposed to a commercial formulation of clomazone (gamit®) |
title |
Parâmetros toxicológicos em carpas (Cyprinus carpio) expostos a uma formulação comercial de clomazone (gamit®) |
spellingShingle |
Parâmetros toxicológicos em carpas (Cyprinus carpio) expostos a uma formulação comercial de clomazone (gamit®) Cattaneo, Roberta Clomazone Toxicidade Rizipiscicultura Carpas Toxicity Rice-fish culture carp CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Parâmetros toxicológicos em carpas (Cyprinus carpio) expostos a uma formulação comercial de clomazone (gamit®) |
title_full |
Parâmetros toxicológicos em carpas (Cyprinus carpio) expostos a uma formulação comercial de clomazone (gamit®) |
title_fullStr |
Parâmetros toxicológicos em carpas (Cyprinus carpio) expostos a uma formulação comercial de clomazone (gamit®) |
title_full_unstemmed |
Parâmetros toxicológicos em carpas (Cyprinus carpio) expostos a uma formulação comercial de clomazone (gamit®) |
title_sort |
Parâmetros toxicológicos em carpas (Cyprinus carpio) expostos a uma formulação comercial de clomazone (gamit®) |
author |
Cattaneo, Roberta |
author_facet |
Cattaneo, Roberta |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Loro, Vania Lucia |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6392817606416780 |
dc.contributor.referee1.fl_str_mv |
Moreira, Heden Luiz Marques |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1229541551615355 |
dc.contributor.referee2.fl_str_mv |
Barcellos, Leonardo José Gil |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/4504482634911546 |
dc.contributor.referee3.fl_str_mv |
Barbosa, Nilda Berenice de Vargas |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/5901511067144019 |
dc.contributor.referee4.fl_str_mv |
Rubin, Maribel Antonello |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/7237734243628134 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4282856921907832 |
dc.contributor.author.fl_str_mv |
Cattaneo, Roberta |
contributor_str_mv |
Loro, Vania Lucia Moreira, Heden Luiz Marques Barcellos, Leonardo José Gil Barbosa, Nilda Berenice de Vargas Rubin, Maribel Antonello |
dc.subject.por.fl_str_mv |
Clomazone Toxicidade Rizipiscicultura Carpas |
topic |
Clomazone Toxicidade Rizipiscicultura Carpas Toxicity Rice-fish culture carp CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Toxicity Rice-fish culture carp |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Commercial formulations of the herbicide clomazone have been widely used in agriculture and fish farming to control weeds. Fish can be affected when the water reaches the drainage waterways, causing an imbalance in the aquatic ecosystem. In order to evaluate a possible contamination, we determined LC50 (96h) using a commercial formulation containing clomazone (Gamit®). We verified the metabolic, enzymatic, and genotoxic parameters as well as the oxidative stress in juvenile carp (Cyprinus carpio). Firstly, in order to determine the LC50 for the acute toxicity test, , the animals were exposed to the concentrations of 10, 20, 30, 40 and 50 mg/L of clomazone for 96 h and the fish behavior was analyzed during this period. After exposure, we verified the activity of the enzyme acetylcholinesterase (AChE) in muscle and brain of the dead fish and the live carp. Secondly, fish were exposed to the herbicide for 7 days, both in field (rice crop) and laboratory conditions. The concentration used in the rice crop and the laboratory was 0.5 mg/L. After the experimental period of 7 days under laboratory conditions and 7, 30, and 90 days under rice crop conditions, brain, liver and muscle of fish were sampled to carry out the toxicological analysis. The parameters studied were enzymatic activity of AChE, catalase (CAT) and glutathione S-tranferase (GST) in different tissues of this species. Some markers of oxidative stress, such as protein carbonylation and thiobarbituric acid reactive substances (TBARS) levels in liver tissue were also analyzed. Some metabolic parameters such as glucose, glycogen, lactate, protein, and ammonia as well as amino acids in liver and muscle of carp were also measured. In plasma, we measured glucose, lactate, and protein. Thirdly, carp were exposed to approximately 15% of the LC50 (5.0 mg/L) for 7 days. We verified the formation of reactive oxygen species (ROS) and micronucleus, and the comet test was performed in order to investigate the presence of DNA damage. In the first study we observed that fish exposed to 20, 30, 40 and 50 mg/L showed behavioral changes and LC50 (96h) was 30.35 mg/L. In addition, it was found that the activity of the enzyme AChE showed no significant changes in the brain of the fish that died at the concentrations tested (30, 40 and 50 mg/L), and the muscle of dead fish had an increase in this enzyme when they were exposed to 50 mg/L of clomazone. The activity decreased significantly in the brain AChE of fish that remained alive after 96 h of exposure to 10, 20 and 30 mg/L, but increased in the muscle of the surviving fish exposed to all concentrations tested. In the second experiment results showed that fish exposed to 7 days had no changes in AChE under field conditions. However, a decrease in the activity of this enzyme in muscle was observed under laboratory conditions. During the same period of exposition, the parameters of oxidative stress conditions changed both under the field and in laboratory conditions. However, metabolic parameters were altered only under field conditions. After 30 and 90 days, AChE activity did not change under field conditions. Disturbances in oxidative stress parameters and metabolism in different tissues were evident in the tissues up to 90 days after treatment. The results showed that the activity of AChE changed only under laboratory conditions, and that oxidative stress associated with metabolic parameters can be good indicators of contamination for clomazone in C. Carpio in rice field conditions. In the third experiment, the results showed an increased formation of ROS and a significant micronucleus (MN) and DNA damage in erythrocytes after exposure to 5.0 mg/L of the herbicide clomazone. Given these results, we can conclude that the herbicide studied can be dangerous to Cyprinus carpio when exposed to it due to increased ROS, which in turn cause oxidative stress evidenced by changes in enzyme markers, metabolic, genotoxic and oxidative stress. However, further studies are needed in order to verify the safety of this herbicide is when associating rice and fish. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-10-07 |
dc.date.accessioned.fl_str_mv |
2017-04-26 |
dc.date.available.fl_str_mv |
2017-04-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CATTANEO, Roberta. Toxicological parameters in carp (Cyprinus carpio) exposed to a commercial formulation of clomazone (gamit®). 2011. 146 f. Tese (Doutorado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/4435 |
identifier_str_mv |
CATTANEO, Roberta. Toxicological parameters in carp (Cyprinus carpio) exposed to a commercial formulation of clomazone (gamit®). 2011. 146 f. Tese (Doutorado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
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http://repositorio.ufsm.br/handle/1/4435 |
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por |
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Universidade Federal de Santa Maria |
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Bioquímica |
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Universidade Federal de Santa Maria |
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