Atividade das ecto-enzimas em plaquetas de ratos com hipercolesterolemia induzida tratados com curcumina e submetidos ao exercício físico
Ano de defesa: | 2012 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
|
Departamento: |
Bioquímica
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/11200 |
Resumo: | Hypercholesterolemia is a risk factor for the development of atherosclerosis, which is a chronic inflammatory disease characterized by accumulation of platelets on the walls of arteries and also by an inflammatory process. Platelets accumulated within atherosclerotic lesions, may recruit additional platelets to form a thrombus. Extracellular nucleotides and nucleosides such as ATP, ADP, AMP and adenosine have important functions, including the regulation of the thrombus, causing a variety of effects on platelets. The family of ecto-enzymes (E-NTPDase, E-5'-nucleotidase, E-NPP, E-ADA) that regulates the concentration of these nucleotides and nucleosides. Studies show that physical exercise may cause changes in lipid profile. Among the exercises, swimming in rats has been used to evaluate cardiovascular adaptations to exercise, may be an adjunct therapy to prevent progression of atherosclerosis. In the exercise, natural products have been used for the control of cardiovascular diseases, such as curcumin, a natural phenolic compound, which has antioxidant and hypocholesterolemic. Therefore, the objective of this study was to evaluate the activity of ecto-enzymes E-NTPDase, E-5'-nucleotidase, E-NPP and E- ADA in platelets of rats with induced hypercholesterolemia treated with curcumin and submitted to physical exercise. The rats used in the study were divided into eight groups: standard diet (C), standard diet along with curcumin (CC), standard diet along with physical exercise (PE), standard diet along with curcumin and physical exercise (CCPE), hypercholesterolemic diet (H), hypercholesterolemic diet along with curcumin (HCC), hypercholesterolemic diet along with physical exercise (HPE), and hypercholesterolemic diet along with curcumin and physical exercise (HCCPE). The activity of E-NTPDase, E-NPP, E-5'-NT and E-ADA were measured in isolated platelets. Curcumin was administered by gavage at a dose of 25 mg / kg / day, the rats were submitted to forced swimming. There was an increase in the hydrolysis of ATP and ADP in rats with hypercholesterolaemia induced. When these mice were treated with curcumin and physical exercise, physical exercise per se was able to further enhance the hydrolysis of ATP. In the hydrolysis of ADP, it was observed a decrease in activity of the E- NTPDase when hypercholesterolemic rats were treated with curcumin and physical exercise per se, well as when it was associated with curcumin and physical exercise. The activities of E-5'nucleotidase, E-NPP and E-ADA and platelet aggregation, did not show any significant. These results suggest that the hypercholesterolemic diet alters the activity of ecto-enzyme in platelets, and that treatment with curcumin and physical exercise may be able to modulate hydrolysis of nucleotides and nucleosides of adenine this experimental condition. |
id |
UFSM_a144cc6250015e90989630a51affed03 |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/11200 |
network_acronym_str |
UFSM |
network_name_str |
Biblioteca Digital de Teses e Dissertações do UFSM |
repository_id_str |
|
spelling |
2013-05-272013-05-272012-07-31SCHLEMMER, Josiane Bizzi. ACTIVITIES OF ECTOENZYMES IN PLATELETS IN RATS WITH INDUCED HYPERCHOLESTEROLEMIA TRARTED WITH CURCUMIN AND SUBMITTED TO PHYSICAL EXERCISE. 2012. 100 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/11200Hypercholesterolemia is a risk factor for the development of atherosclerosis, which is a chronic inflammatory disease characterized by accumulation of platelets on the walls of arteries and also by an inflammatory process. Platelets accumulated within atherosclerotic lesions, may recruit additional platelets to form a thrombus. Extracellular nucleotides and nucleosides such as ATP, ADP, AMP and adenosine have important functions, including the regulation of the thrombus, causing a variety of effects on platelets. The family of ecto-enzymes (E-NTPDase, E-5'-nucleotidase, E-NPP, E-ADA) that regulates the concentration of these nucleotides and nucleosides. Studies show that physical exercise may cause changes in lipid profile. Among the exercises, swimming in rats has been used to evaluate cardiovascular adaptations to exercise, may be an adjunct therapy to prevent progression of atherosclerosis. In the exercise, natural products have been used for the control of cardiovascular diseases, such as curcumin, a natural phenolic compound, which has antioxidant and hypocholesterolemic. Therefore, the objective of this study was to evaluate the activity of ecto-enzymes E-NTPDase, E-5'-nucleotidase, E-NPP and E- ADA in platelets of rats with induced hypercholesterolemia treated with curcumin and submitted to physical exercise. The rats used in the study were divided into eight groups: standard diet (C), standard diet along with curcumin (CC), standard diet along with physical exercise (PE), standard diet along with curcumin and physical exercise (CCPE), hypercholesterolemic diet (H), hypercholesterolemic diet along with curcumin (HCC), hypercholesterolemic diet along with physical exercise (HPE), and hypercholesterolemic diet along with curcumin and physical exercise (HCCPE). The activity of E-NTPDase, E-NPP, E-5'-NT and E-ADA were measured in isolated platelets. Curcumin was administered by gavage at a dose of 25 mg / kg / day, the rats were submitted to forced swimming. There was an increase in the hydrolysis of ATP and ADP in rats with hypercholesterolaemia induced. When these mice were treated with curcumin and physical exercise, physical exercise per se was able to further enhance the hydrolysis of ATP. In the hydrolysis of ADP, it was observed a decrease in activity of the E- NTPDase when hypercholesterolemic rats were treated with curcumin and physical exercise per se, well as when it was associated with curcumin and physical exercise. The activities of E-5'nucleotidase, E-NPP and E-ADA and platelet aggregation, did not show any significant. These results suggest that the hypercholesterolemic diet alters the activity of ecto-enzyme in platelets, and that treatment with curcumin and physical exercise may be able to modulate hydrolysis of nucleotides and nucleosides of adenine this experimental condition.A hipercolesterolemia é um fator de risco para o surgimento da aterosclerose, sendo esta uma doença inflamatória crônica caracterizada por acúmulo de plaquetas nas paredes das artérias e também por um processo inflamatório. As plaquetas acumuladas dentro das lesões ateroscleróticas, podem recrutar plaquetas adicionais para formar um trombo. Os nucleotídeos e nucleosídeos extracelulares como ATP, ADP, AMP e adenosina têm funções importantes, incluindo a regulação do trombo, exercendo uma variedade de efeitos sobre as plaquetas. A família de ectoenzimas (E-NTPDase, E-5'-nucleotidase, E-NPP, E-ADA) é que regula a concentração destes nucleotídeos e nucleosídeos. Estudos demonstram que o exercício físico, pode provocar alterações no perfil lipídico. Dentre os exercícios físicos, o nado tem sido utilizado em ratos para avaliação de adaptações cardiovasculares ao exercício, podendo ser uma terapia adjuvante para a prevenção da progressão da aterosclerose. Além do exercício físico, produtos naturais tem sido utilizado para o controle de doenças cardiovasculares, como é o caso da curcumina, um composto fenólico natural, que tem propriedades antioxidantes e hipocolesterolêmicas. Diante disso, o objetivo deste trabalho foi avaliar a atividade das ectoenzimas E-NTPDase, E-5 -nucleotidase, E-NPP e E-ADA em plaquetas de ratos com hipercolesterolemia induzida tratados com curcumina e submetidos ao exercício físico. Os ratos utilizados no estudo foram divididos em 8 grupos: dieta padrão (C), dieta padrão mais curcumina (CC), dieta padrão mais exercício físico (PE), dieta padrão mais exercício físico e curcumina (CCPE), dieta hipercolesterolêmica (H), dieta hipercolesterolêmica mais curcumina (HCC), dieta hipercolesterolêmica mais exercício físico (HPE), e dieta hipercolesterolêmica mais exercício físico e curcumina (HCCPE). A atividade da E-NTPDase, E-NPP, E-5'-NT e E-ADA foram medidos em plaquetas isoladas. A curcumina foi administrada por gavagem na dose de 25 mg/kg/dia e os ratos foram submetidos ao nado forçado. Foi observado um aumento na hidrólise do ATP e ADP nos ratos com hipercolesterolemia induzida. Quando esses ratos receberam tratamento com curcumina e exercício físico, o exercício físico por si só foi capaz de aumentar a hidrólise de ATP. Na hidrólise de ADP, observou-se uma diminuição na atividade da E-NTPDase quando os ratos hipercolesterolêmicos foram tratados com curcumina e exercício físico por si só, bem como quando foram tratados com curcumina e exercício físico concomitante. A atividade da E-5'nucleotidase, E-NPP, E-ADA e agregação plaquetária não mostraram diferenças significativas. Estes resultados sugerem que a dieta hipercolesterolêmica altera a atividade das ecto-enzimas em plaquetas, e que o tratamento com a curcumina e exercício físico pode ser capaz de modular a hidrólise de nucleotídeos e nucleosídeos de adenina nesta condição experimental.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaHipercolesterolemiaCurcuminaExercício físicoSistema purinérgicoHypercholesterolemiaCurcuminPhysical exercisePurinergic systemCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAAtividade das ecto-enzimas em plaquetas de ratos com hipercolesterolemia induzida tratados com curcumina e submetidos ao exercício físicoActivities of ectoenzymes in platelets in rats with induced hypercholesterolemia trarted with curcumin and submitted to physical exerciseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisLeal, Daniela Bitencourt Rosahttp://lattes.cnpq.br/3639683273462361Oliveira, Edilamar Menezes dehttp://lattes.cnpq.br/0764729178520349Cruz, Ivana Beatrice Mânica dahttp://lattes.cnpq.br/3426369324110716http://lattes.cnpq.br/0217671314699365Schlemmer, Josiane Bizzi200800000002400500300300500ebd6d0bc-4e14-43fd-8d4e-ad9ebe640af2dcb0918c-6790-4edf-87da-2ada6e780b77db4e9863-c772-46d9-8246-261f779a19ad3e70f8d1-28e2-435e-ae3a-51a0a48a38cdinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALSCHLEMMER, JOSIANE BIZZI.pdfapplication/pdf1620936http://repositorio.ufsm.br/bitstream/1/11200/1/SCHLEMMER%2c%20JOSIANE%20BIZZI.pdfc1c4d8d45c80effd10675fbe3b59a92eMD51TEXTSCHLEMMER, JOSIANE BIZZI.pdf.txtSCHLEMMER, JOSIANE BIZZI.pdf.txtExtracted texttext/plain185965http://repositorio.ufsm.br/bitstream/1/11200/2/SCHLEMMER%2c%20JOSIANE%20BIZZI.pdf.txtb27ca9d63e53ce372cae53c3d9dee1d8MD52THUMBNAILSCHLEMMER, JOSIANE BIZZI.pdf.jpgSCHLEMMER, JOSIANE BIZZI.pdf.jpgIM Thumbnailimage/jpeg5098http://repositorio.ufsm.br/bitstream/1/11200/3/SCHLEMMER%2c%20JOSIANE%20BIZZI.pdf.jpg9ac6c77cf668653305eadc097c5adf9eMD531/112002022-07-22 12:12:38.348oai:repositorio.ufsm.br:1/11200Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-07-22T15:12:38Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Atividade das ecto-enzimas em plaquetas de ratos com hipercolesterolemia induzida tratados com curcumina e submetidos ao exercício físico |
dc.title.alternative.eng.fl_str_mv |
Activities of ectoenzymes in platelets in rats with induced hypercholesterolemia trarted with curcumin and submitted to physical exercise |
title |
Atividade das ecto-enzimas em plaquetas de ratos com hipercolesterolemia induzida tratados com curcumina e submetidos ao exercício físico |
spellingShingle |
Atividade das ecto-enzimas em plaquetas de ratos com hipercolesterolemia induzida tratados com curcumina e submetidos ao exercício físico Schlemmer, Josiane Bizzi Hipercolesterolemia Curcumina Exercício físico Sistema purinérgico Hypercholesterolemia Curcumin Physical exercise Purinergic system CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Atividade das ecto-enzimas em plaquetas de ratos com hipercolesterolemia induzida tratados com curcumina e submetidos ao exercício físico |
title_full |
Atividade das ecto-enzimas em plaquetas de ratos com hipercolesterolemia induzida tratados com curcumina e submetidos ao exercício físico |
title_fullStr |
Atividade das ecto-enzimas em plaquetas de ratos com hipercolesterolemia induzida tratados com curcumina e submetidos ao exercício físico |
title_full_unstemmed |
Atividade das ecto-enzimas em plaquetas de ratos com hipercolesterolemia induzida tratados com curcumina e submetidos ao exercício físico |
title_sort |
Atividade das ecto-enzimas em plaquetas de ratos com hipercolesterolemia induzida tratados com curcumina e submetidos ao exercício físico |
author |
Schlemmer, Josiane Bizzi |
author_facet |
Schlemmer, Josiane Bizzi |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Leal, Daniela Bitencourt Rosa |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3639683273462361 |
dc.contributor.referee1.fl_str_mv |
Oliveira, Edilamar Menezes de |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/0764729178520349 |
dc.contributor.referee2.fl_str_mv |
Cruz, Ivana Beatrice Mânica da |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/3426369324110716 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0217671314699365 |
dc.contributor.author.fl_str_mv |
Schlemmer, Josiane Bizzi |
contributor_str_mv |
Leal, Daniela Bitencourt Rosa Oliveira, Edilamar Menezes de Cruz, Ivana Beatrice Mânica da |
dc.subject.por.fl_str_mv |
Hipercolesterolemia Curcumina Exercício físico Sistema purinérgico |
topic |
Hipercolesterolemia Curcumina Exercício físico Sistema purinérgico Hypercholesterolemia Curcumin Physical exercise Purinergic system CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Hypercholesterolemia Curcumin Physical exercise Purinergic system |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Hypercholesterolemia is a risk factor for the development of atherosclerosis, which is a chronic inflammatory disease characterized by accumulation of platelets on the walls of arteries and also by an inflammatory process. Platelets accumulated within atherosclerotic lesions, may recruit additional platelets to form a thrombus. Extracellular nucleotides and nucleosides such as ATP, ADP, AMP and adenosine have important functions, including the regulation of the thrombus, causing a variety of effects on platelets. The family of ecto-enzymes (E-NTPDase, E-5'-nucleotidase, E-NPP, E-ADA) that regulates the concentration of these nucleotides and nucleosides. Studies show that physical exercise may cause changes in lipid profile. Among the exercises, swimming in rats has been used to evaluate cardiovascular adaptations to exercise, may be an adjunct therapy to prevent progression of atherosclerosis. In the exercise, natural products have been used for the control of cardiovascular diseases, such as curcumin, a natural phenolic compound, which has antioxidant and hypocholesterolemic. Therefore, the objective of this study was to evaluate the activity of ecto-enzymes E-NTPDase, E-5'-nucleotidase, E-NPP and E- ADA in platelets of rats with induced hypercholesterolemia treated with curcumin and submitted to physical exercise. The rats used in the study were divided into eight groups: standard diet (C), standard diet along with curcumin (CC), standard diet along with physical exercise (PE), standard diet along with curcumin and physical exercise (CCPE), hypercholesterolemic diet (H), hypercholesterolemic diet along with curcumin (HCC), hypercholesterolemic diet along with physical exercise (HPE), and hypercholesterolemic diet along with curcumin and physical exercise (HCCPE). The activity of E-NTPDase, E-NPP, E-5'-NT and E-ADA were measured in isolated platelets. Curcumin was administered by gavage at a dose of 25 mg / kg / day, the rats were submitted to forced swimming. There was an increase in the hydrolysis of ATP and ADP in rats with hypercholesterolaemia induced. When these mice were treated with curcumin and physical exercise, physical exercise per se was able to further enhance the hydrolysis of ATP. In the hydrolysis of ADP, it was observed a decrease in activity of the E- NTPDase when hypercholesterolemic rats were treated with curcumin and physical exercise per se, well as when it was associated with curcumin and physical exercise. The activities of E-5'nucleotidase, E-NPP and E-ADA and platelet aggregation, did not show any significant. These results suggest that the hypercholesterolemic diet alters the activity of ecto-enzyme in platelets, and that treatment with curcumin and physical exercise may be able to modulate hydrolysis of nucleotides and nucleosides of adenine this experimental condition. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-07-31 |
dc.date.accessioned.fl_str_mv |
2013-05-27 |
dc.date.available.fl_str_mv |
2013-05-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SCHLEMMER, Josiane Bizzi. ACTIVITIES OF ECTOENZYMES IN PLATELETS IN RATS WITH INDUCED HYPERCHOLESTEROLEMIA TRARTED WITH CURCUMIN AND SUBMITTED TO PHYSICAL EXERCISE. 2012. 100 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/11200 |
identifier_str_mv |
SCHLEMMER, Josiane Bizzi. ACTIVITIES OF ECTOENZYMES IN PLATELETS IN RATS WITH INDUCED HYPERCHOLESTEROLEMIA TRARTED WITH CURCUMIN AND SUBMITTED TO PHYSICAL EXERCISE. 2012. 100 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
url |
http://repositorio.ufsm.br/handle/1/11200 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
200800000002 |
dc.relation.confidence.fl_str_mv |
400 500 300 300 500 |
dc.relation.authority.fl_str_mv |
ebd6d0bc-4e14-43fd-8d4e-ad9ebe640af2 dcb0918c-6790-4edf-87da-2ada6e780b77 db4e9863-c772-46d9-8246-261f779a19ad 3e70f8d1-28e2-435e-ae3a-51a0a48a38cd |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Bioquímica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações do UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Biblioteca Digital de Teses e Dissertações do UFSM |
collection |
Biblioteca Digital de Teses e Dissertações do UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/11200/1/SCHLEMMER%2c%20JOSIANE%20BIZZI.pdf http://repositorio.ufsm.br/bitstream/1/11200/2/SCHLEMMER%2c%20JOSIANE%20BIZZI.pdf.txt http://repositorio.ufsm.br/bitstream/1/11200/3/SCHLEMMER%2c%20JOSIANE%20BIZZI.pdf.jpg |
bitstream.checksum.fl_str_mv |
c1c4d8d45c80effd10675fbe3b59a92e b27ca9d63e53ce372cae53c3d9dee1d8 9ac6c77cf668653305eadc097c5adf9e |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1801485429383364608 |