Conversão fermentativa de microalga Chlorella sp. em goma gelana por Sphingomonas paucimobilis
Ano de defesa: | 2020 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Química
|
Departamento: |
Química
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/23084 |
Resumo: | Microalgae (MA) are potential feedstock for the production of fermentable monosaccharide, due to the appreciable carbohydrate fraction. The species used in this dissertation was Chlorella sp., one of the most resistant kind of MA. Thus, this research work aimed to convert MA biomass in gellan gum (GG) by fermentation with Sphingomonas paucimobilis. The physical-chemical treatment applied was pressurized acid hydrolysis, to weakening the biomass structure and making available fermentable sugars from the cellulosic fractions. The best results for microalgae (MA) in natura were 12.3 g of sugar L-1 for the pressurized sulfuric hydrolysis (150 °C, 2.5% v v-1, 36.6 min) and 12.5 g of sugars L-1 for the pressurized hydrochloric hydrolysis (155 °C, 1.5% v v-1, 40 min). Activated carbon (50 ºC, 10% m v-1, 60 min) was used to detoxify MA hydrolyzate, making possible the use of this hydrolyzate as fermentative medium for Sphingomonas paucimobilis. Using multivariate approach to optimize the culture conditions, temperature (20-30 ºC) and orbital agitation (120-240 rpm) of the fermentation stage were evaluated. Under optimized conditions (pH 7.0, 7.0 g yeast extract L-1, 30 ºC, 240 rpm) the production of GG, extracted with cooled ethyl alcohol, resulted in 53.3 g L-1 of hydrolyzate, after 60 h of cultivation. The analytical method make use of a High Performance Liquid Chromatograph coupled to Refractive Index Detector (HPLC-RID), applying figure-of-merit and method validation studies. The characterization of GG was done by Fourier Transform Infrared Spectrometry (FT-IR) and Gel Permeation Chromatography (GPC). The viability of using MA for sugar production was demonstrated on lab scale, and, the viability of bioconversion into GG (91.7% purity), throughout an innovative production route. Adittionally, a preliminary economic evaluation of the process was provided. |
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2021-12-01T12:04:21Z2021-12-01T12:04:21Z2020-01-30http://repositorio.ufsm.br/handle/1/23084Microalgae (MA) are potential feedstock for the production of fermentable monosaccharide, due to the appreciable carbohydrate fraction. The species used in this dissertation was Chlorella sp., one of the most resistant kind of MA. Thus, this research work aimed to convert MA biomass in gellan gum (GG) by fermentation with Sphingomonas paucimobilis. The physical-chemical treatment applied was pressurized acid hydrolysis, to weakening the biomass structure and making available fermentable sugars from the cellulosic fractions. The best results for microalgae (MA) in natura were 12.3 g of sugar L-1 for the pressurized sulfuric hydrolysis (150 °C, 2.5% v v-1, 36.6 min) and 12.5 g of sugars L-1 for the pressurized hydrochloric hydrolysis (155 °C, 1.5% v v-1, 40 min). Activated carbon (50 ºC, 10% m v-1, 60 min) was used to detoxify MA hydrolyzate, making possible the use of this hydrolyzate as fermentative medium for Sphingomonas paucimobilis. Using multivariate approach to optimize the culture conditions, temperature (20-30 ºC) and orbital agitation (120-240 rpm) of the fermentation stage were evaluated. Under optimized conditions (pH 7.0, 7.0 g yeast extract L-1, 30 ºC, 240 rpm) the production of GG, extracted with cooled ethyl alcohol, resulted in 53.3 g L-1 of hydrolyzate, after 60 h of cultivation. The analytical method make use of a High Performance Liquid Chromatograph coupled to Refractive Index Detector (HPLC-RID), applying figure-of-merit and method validation studies. The characterization of GG was done by Fourier Transform Infrared Spectrometry (FT-IR) and Gel Permeation Chromatography (GPC). The viability of using MA for sugar production was demonstrated on lab scale, and, the viability of bioconversion into GG (91.7% purity), throughout an innovative production route. Adittionally, a preliminary economic evaluation of the process was provided.As microalgas (MAs) são potenciais matérias-primas na produção de monossacarídeos fermentescíveis, devido ao apreciável teor de carboidratos. A espécie usada neste trabalho foi Chlorella sp., uma das variedades de MA mais resistentes ao cultivo. Assim sendo, este trabalho objetivou a conversão desta biomassa em goma gelana (GG) por fermentação com Sphingomonas paucimobilis. O tratamento físico-químico usado foi hidrólise ácida à pressão, cuja atuação enfraquece a estrutura da biomassa e libera açúcares fermentescíveis das frações celulósicas dessa matriz. Os melhores resultados para a microalga (MA) in natura foram 12,3 g açúcares L-1, usando-se H2SO4 (150 °C, 2,5% v v-1 e 36,6 min), e 12,5 g açúcares L-1, com o uso de HCl (155 °C, 1,5% v v-1 e 40 min). Carbono ativado comercial (50 ºC, 10% m v-1 e 60 min) foi empregado na etapa de destoxificação do hidrolisado de MA, tornando possível o uso deste hidrolisado enquanto meio fermentativo para Sphingomonas paucimobilis. Com abordagem multivariada às condições de cultivo, avaliou-se a temperatura (20–30 ºC) e a agitação orbital (120–240 rpm) da etapa fermentativa. Nas condições otimizadas (pH 7,0, 7,0 g de extrato de levedura L-1, 30 ºC e 240 rpm) a produção de GG (após extração com álcool etílico resfriado), foi de até 53,3 g L-1 de hidrolisado, em 60 h de cultivo. A técnica analítica utilizada foi Cromatografia Líquida de Alta Eficiência com Detecção por Índice de Refração (HPLC-RID), submetida a estudo de figuras-de-mérito e de validação de método. A caracterização da GG foi feita por técnicas de Espectrometria de Infravermelho com Transformada de Fourier (FT-IR) e Cromatografia de Permeação em Gel (GPC). A viabilidade do aproveitamento de MA para produção de açúcares foi demonstrada, em escala de laboratório, bem como a viabilidade da bioconversão em GG (91,7% de pureza), por meio de rota produtiva inovadora. Adicionalmente, fez-se uma análise econômica preliminar do processo.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em QuímicaUFSMBrasilQuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessMicroalgaHidrólise ácida à pressãoSphingomonas paucimobilisGoma gelanaOtimização multivariadaMicroalgaePressurized acid hydrolysisGellan gumMultivariate optimizationCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICAConversão fermentativa de microalga Chlorella sp. em goma gelana por Sphingomonas paucimobilisFermentative conversion of Chlorella sp. microalgae in gellan gum by Sphingomonas paucimobilisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMartins, Ayrton Figueiredohttp://lattes.cnpq.br/2113532494494821D'Oca, Marcelo Gonçalves MontesDenardi, Laura Bedinhttp://lattes.cnpq.br/7631468700181864Saldanha, Loisleini Fontoura100600000000600600600600600ade6000d-a348-4d49-a531-6500a65a153aa90864a9-f369-41ca-8189-5c57a5cf1fbce36017b4-6361-4ccc-ae10-c7da957c957a3546cbe2-d401-4799-9ddc-45b4813c83b5reponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGQUIMICA_2020_SALDANHA_LOISLEINI.pdfDIS_PPGQUIMICA_2020_SALDANHA_LOISLEINI.pdfDissertaçãoapplication/pdf1999585http://repositorio.ufsm.br/bitstream/1/23084/1/DIS_PPGQUIMICA_2020_SALDANHA_LOISLEINI.pdf7cdc18d50aea1a93365a2f5554d2d312MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Conversão fermentativa de microalga Chlorella sp. em goma gelana por Sphingomonas paucimobilis |
dc.title.alternative.eng.fl_str_mv |
Fermentative conversion of Chlorella sp. microalgae in gellan gum by Sphingomonas paucimobilis |
title |
Conversão fermentativa de microalga Chlorella sp. em goma gelana por Sphingomonas paucimobilis |
spellingShingle |
Conversão fermentativa de microalga Chlorella sp. em goma gelana por Sphingomonas paucimobilis Saldanha, Loisleini Fontoura Microalga Hidrólise ácida à pressão Sphingomonas paucimobilis Goma gelana Otimização multivariada Microalgae Pressurized acid hydrolysis Gellan gum Multivariate optimization CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Conversão fermentativa de microalga Chlorella sp. em goma gelana por Sphingomonas paucimobilis |
title_full |
Conversão fermentativa de microalga Chlorella sp. em goma gelana por Sphingomonas paucimobilis |
title_fullStr |
Conversão fermentativa de microalga Chlorella sp. em goma gelana por Sphingomonas paucimobilis |
title_full_unstemmed |
Conversão fermentativa de microalga Chlorella sp. em goma gelana por Sphingomonas paucimobilis |
title_sort |
Conversão fermentativa de microalga Chlorella sp. em goma gelana por Sphingomonas paucimobilis |
author |
Saldanha, Loisleini Fontoura |
author_facet |
Saldanha, Loisleini Fontoura |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Martins, Ayrton Figueiredo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2113532494494821 |
dc.contributor.referee1.fl_str_mv |
D'Oca, Marcelo Gonçalves Montes |
dc.contributor.referee2.fl_str_mv |
Denardi, Laura Bedin |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7631468700181864 |
dc.contributor.author.fl_str_mv |
Saldanha, Loisleini Fontoura |
contributor_str_mv |
Martins, Ayrton Figueiredo D'Oca, Marcelo Gonçalves Montes Denardi, Laura Bedin |
dc.subject.por.fl_str_mv |
Microalga Hidrólise ácida à pressão Sphingomonas paucimobilis Goma gelana Otimização multivariada |
topic |
Microalga Hidrólise ácida à pressão Sphingomonas paucimobilis Goma gelana Otimização multivariada Microalgae Pressurized acid hydrolysis Gellan gum Multivariate optimization CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Microalgae Pressurized acid hydrolysis Gellan gum Multivariate optimization |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
Microalgae (MA) are potential feedstock for the production of fermentable monosaccharide, due to the appreciable carbohydrate fraction. The species used in this dissertation was Chlorella sp., one of the most resistant kind of MA. Thus, this research work aimed to convert MA biomass in gellan gum (GG) by fermentation with Sphingomonas paucimobilis. The physical-chemical treatment applied was pressurized acid hydrolysis, to weakening the biomass structure and making available fermentable sugars from the cellulosic fractions. The best results for microalgae (MA) in natura were 12.3 g of sugar L-1 for the pressurized sulfuric hydrolysis (150 °C, 2.5% v v-1, 36.6 min) and 12.5 g of sugars L-1 for the pressurized hydrochloric hydrolysis (155 °C, 1.5% v v-1, 40 min). Activated carbon (50 ºC, 10% m v-1, 60 min) was used to detoxify MA hydrolyzate, making possible the use of this hydrolyzate as fermentative medium for Sphingomonas paucimobilis. Using multivariate approach to optimize the culture conditions, temperature (20-30 ºC) and orbital agitation (120-240 rpm) of the fermentation stage were evaluated. Under optimized conditions (pH 7.0, 7.0 g yeast extract L-1, 30 ºC, 240 rpm) the production of GG, extracted with cooled ethyl alcohol, resulted in 53.3 g L-1 of hydrolyzate, after 60 h of cultivation. The analytical method make use of a High Performance Liquid Chromatograph coupled to Refractive Index Detector (HPLC-RID), applying figure-of-merit and method validation studies. The characterization of GG was done by Fourier Transform Infrared Spectrometry (FT-IR) and Gel Permeation Chromatography (GPC). The viability of using MA for sugar production was demonstrated on lab scale, and, the viability of bioconversion into GG (91.7% purity), throughout an innovative production route. Adittionally, a preliminary economic evaluation of the process was provided. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-01-30 |
dc.date.accessioned.fl_str_mv |
2021-12-01T12:04:21Z |
dc.date.available.fl_str_mv |
2021-12-01T12:04:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/23084 |
url |
http://repositorio.ufsm.br/handle/1/23084 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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100600000000 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 |
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dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Química |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Química |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
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