Classificação filogenética e caracterização patotípica de isolados de Escherichia coli patogênicos e comensais de suínos da região Sul do Brasil
Ano de defesa: | 2009 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Medicina Veterinária
|
Departamento: |
Medicina Veterinária
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/10038 |
Resumo: | The aim of the study was to evaluate the presence of different virulence factors in intestinal and extra-intestinal Escherichia coli swine isolates from Southern Brazil by multiplex PCR (mPCR) and classify them in A, B1, B2 and D phylogenetic groups based on three pathogenicity markers: chuA, yjaA and a DNA anonymous fragment designated TSPE4.C2. Were analyzed 152 samples from different origins: urine (70), feces (35), small intestine (35) and tissues (12). The mPCR was carried out to pathotypification of the isolates and a PCR with the pathogenicity markers, to classify in phylogenetic groups. Seventy seven (51%) isolates when tested by mPCR were positive to at least one of the virulence factors, while the phylogenetic characterization of the isolates showed 21 (14%) isolates in A, 65 (42%) in B1, 19 (13%) in B2 and 47 (31%) in D group. Fourteen (20%) urine isolates were characterized as UPEC; nine (12.8%) presented UPEC and ETEC factors simultaneously and four (5.8%) were classified as ETEC. In the phylogenetic classification, the groups of major occurrence were D (45.8%) and B1 (32.8%). Of the analyzed feces samples, 25 (71.4%) demonstrated virulence factors characteristic of pathotype ETEC. Phylogeneticaly, the group of higher incidence was B1 with 21 (60%) samples, followed by B2 with six (17.2%), group A with five (14.2%) and group D with three (8.6%) isolates. For the small intestine samples, 20 (57.2%) were characterized as ETEC. By the phylogeny, 23 (65.6%) isolates were classified in groups A or B1, among them 51.4% belonged to B1 and six (17.2%) were located in equal percentage in groups B2 and D. Six tissue isolates (50%) were qualified as ETEC. In regards to the phylogenetic classification, six (50%) samples were located in group D, followed by A and B1, with three (50%) samples in each group. For the tested isolates, the phylogenetic analysis demonstrated to be efficient, and it may be included in laboratorial routine for epidemiologic studies of the intestinal and extra intestinal swine samples, allowing the differentiation of pathogenic and non-pathogenic isolates, as well as helping in the strains selection for the vaccine production. |
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2017-06-132017-06-132009-02-18KOLLING, Lilian. Phylogenetic classification and pathotype characterization of pathogenic and commensal Escherichia coli isolated of swine from Southern Brazil. 2009. 55 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2009.http://repositorio.ufsm.br/handle/1/10038The aim of the study was to evaluate the presence of different virulence factors in intestinal and extra-intestinal Escherichia coli swine isolates from Southern Brazil by multiplex PCR (mPCR) and classify them in A, B1, B2 and D phylogenetic groups based on three pathogenicity markers: chuA, yjaA and a DNA anonymous fragment designated TSPE4.C2. Were analyzed 152 samples from different origins: urine (70), feces (35), small intestine (35) and tissues (12). The mPCR was carried out to pathotypification of the isolates and a PCR with the pathogenicity markers, to classify in phylogenetic groups. Seventy seven (51%) isolates when tested by mPCR were positive to at least one of the virulence factors, while the phylogenetic characterization of the isolates showed 21 (14%) isolates in A, 65 (42%) in B1, 19 (13%) in B2 and 47 (31%) in D group. Fourteen (20%) urine isolates were characterized as UPEC; nine (12.8%) presented UPEC and ETEC factors simultaneously and four (5.8%) were classified as ETEC. In the phylogenetic classification, the groups of major occurrence were D (45.8%) and B1 (32.8%). Of the analyzed feces samples, 25 (71.4%) demonstrated virulence factors characteristic of pathotype ETEC. Phylogeneticaly, the group of higher incidence was B1 with 21 (60%) samples, followed by B2 with six (17.2%), group A with five (14.2%) and group D with three (8.6%) isolates. For the small intestine samples, 20 (57.2%) were characterized as ETEC. By the phylogeny, 23 (65.6%) isolates were classified in groups A or B1, among them 51.4% belonged to B1 and six (17.2%) were located in equal percentage in groups B2 and D. Six tissue isolates (50%) were qualified as ETEC. In regards to the phylogenetic classification, six (50%) samples were located in group D, followed by A and B1, with three (50%) samples in each group. For the tested isolates, the phylogenetic analysis demonstrated to be efficient, and it may be included in laboratorial routine for epidemiologic studies of the intestinal and extra intestinal swine samples, allowing the differentiation of pathogenic and non-pathogenic isolates, as well as helping in the strains selection for the vaccine production.O presente estudo tem por objetivo avaliar a presença de diferentes fatores de virulência em isolados de Escherichia coli intestinais e extra-intestinais provenientes de suínos da região sul do Brasil pela PCR multiplex (mPCR) e classificá-los nos grupos filogenéticos A, B1, B2 e D pela detecção de três marcadores de patogenicidade: chuA, yjaA e um fragmento anônimo de DNA designado TSPE4.C2. Foram analisadas 152 amostras de diferentes origens: urina (70), fezes (35), intestino delgado (35) e tecidos (12). A mPCR foi realizada para a patotipificação dos isolados e uma PCR com os marcadores acima citados, para a classificação dos grupos filogenéticos. Setenta e sete (51%) isolados testados pela mPCR apresentaram-se positivos para ao menos algum fator de virulência, enquanto a caracterização filogenética dos isolados revelou 21 (14%) isolados no grupo A, 65 (42%) em B1, 19 (13%) em B2 e 47 (31%) no grupo D. Quatorze (20%) isolados de urina foram caracterizados como UPEC; nove (12,8%) apresentaram fatores de UPEC e ETEC simultaneamente e quatro (5,8%) foram classificados como ETEC. Na classificação filogenética, os grupos de maior ocorrência foram D (45,8%) e B1 (32,8%). Das amostras de fezes analisadas, 25 (71,4%) demonstraram fatores de virulência característicos do patotipo ETEC. Filogeneticamente, o grupo de maior ocorrência foi o B1 com 21 (60%) amostras, seguido de B2 com seis (17,2%), grupo A com cinco (14,2%) e grupo D com três (8,6%) isolados. Para as amostras de intestino delgado, 20 (57,2%) foram caracterizadas como ETEC. Pela filogenia, 23 (65,6%) isolados classificaram-se nos grupos A ou B1, sendo 51,4% deles neste último e seis (17,2%) foram alocados em iguais proporções nos grupos B2 ou D. Seis isolados de tecidos (50%) foram qualificados como ETEC. Em relação à classificação filogenética, seis (50%) amostras alocaram-se no grupo D, seguidas dos grupos A e B1, com três (25%) amostras em cada grupo. Para os isolados testados, a técnica de análise de filogenia mostrou-se eficiente, podendo ser incluída na rotina laboratorial para levantamento epidemiológico de amostras intestinais e extra-intestinais suínas, permitindo diferenciar assim, isolados patogênicos e não patogênicos, bem como auxiliar na seleção de cepas para confecção de vacinas.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Medicina VeterináriaUFSMBRMedicina VeterináriaFilogeniaPatotipificaçãoE. coliPhylogenyPathotypificationCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAClassificação filogenética e caracterização patotípica de isolados de Escherichia coli patogênicos e comensais de suínos da região Sul do BrasilPhylogenetic classification and pathotype characterization of pathogenic and commensal Escherichia coli isolated of swine from Southern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisVargas, Agueda Palmira Castagna dehttp://lattes.cnpq.br/1383126157031968Cardoso, Marisa Ribeiro de Itapemahttp://lattes.cnpq.br/6781728084964277Botton, Sonia de Avilahttp://lattes.cnpq.br/0814772095155945http://lattes.cnpq.br/3640696048241388Kolling, Lilian5005000000074005003003005007561081c-bfab-4ea7-ace8-2bf6488cb0b505c68291-683d-4f84-a17c-e5eab5fee7768d56be80-3fb1-4900-b5e5-c063175c74ba24806e8f-fdc9-4fdd-9936-d012e1f365bainfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALKOLLING, LILIAN.pdfapplication/pdf341955http://repositorio.ufsm.br/bitstream/1/10038/1/KOLLING%2c%20LILIAN.pdf8455883730efaa91b922bf92447b08e1MD51TEXTKOLLING, LILIAN.pdf.txtKOLLING, LILIAN.pdf.txtExtracted texttext/plain96469http://repositorio.ufsm.br/bitstream/1/10038/2/KOLLING%2c%20LILIAN.pdf.txtb236ebc45afe84652455edac55fdff9aMD52THUMBNAILKOLLING, LILIAN.pdf.jpgKOLLING, LILIAN.pdf.jpgIM Thumbnailimage/jpeg5378http://repositorio.ufsm.br/bitstream/1/10038/3/KOLLING%2c%20LILIAN.pdf.jpga8231107cbec69af624bc47cc0e70001MD531/100382022-06-28 11:48:42.565oai:repositorio.ufsm.br:1/10038Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-06-28T14:48:42Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Classificação filogenética e caracterização patotípica de isolados de Escherichia coli patogênicos e comensais de suínos da região Sul do Brasil |
dc.title.alternative.eng.fl_str_mv |
Phylogenetic classification and pathotype characterization of pathogenic and commensal Escherichia coli isolated of swine from Southern Brazil |
title |
Classificação filogenética e caracterização patotípica de isolados de Escherichia coli patogênicos e comensais de suínos da região Sul do Brasil |
spellingShingle |
Classificação filogenética e caracterização patotípica de isolados de Escherichia coli patogênicos e comensais de suínos da região Sul do Brasil Kolling, Lilian Filogenia Patotipificação E. coli Phylogeny Pathotypification CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Classificação filogenética e caracterização patotípica de isolados de Escherichia coli patogênicos e comensais de suínos da região Sul do Brasil |
title_full |
Classificação filogenética e caracterização patotípica de isolados de Escherichia coli patogênicos e comensais de suínos da região Sul do Brasil |
title_fullStr |
Classificação filogenética e caracterização patotípica de isolados de Escherichia coli patogênicos e comensais de suínos da região Sul do Brasil |
title_full_unstemmed |
Classificação filogenética e caracterização patotípica de isolados de Escherichia coli patogênicos e comensais de suínos da região Sul do Brasil |
title_sort |
Classificação filogenética e caracterização patotípica de isolados de Escherichia coli patogênicos e comensais de suínos da região Sul do Brasil |
author |
Kolling, Lilian |
author_facet |
Kolling, Lilian |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Vargas, Agueda Palmira Castagna de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1383126157031968 |
dc.contributor.referee1.fl_str_mv |
Cardoso, Marisa Ribeiro de Itapema |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6781728084964277 |
dc.contributor.referee2.fl_str_mv |
Botton, Sonia de Avila |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/0814772095155945 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3640696048241388 |
dc.contributor.author.fl_str_mv |
Kolling, Lilian |
contributor_str_mv |
Vargas, Agueda Palmira Castagna de Cardoso, Marisa Ribeiro de Itapema Botton, Sonia de Avila |
dc.subject.por.fl_str_mv |
Filogenia Patotipificação E. coli |
topic |
Filogenia Patotipificação E. coli Phylogeny Pathotypification CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Phylogeny Pathotypification |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The aim of the study was to evaluate the presence of different virulence factors in intestinal and extra-intestinal Escherichia coli swine isolates from Southern Brazil by multiplex PCR (mPCR) and classify them in A, B1, B2 and D phylogenetic groups based on three pathogenicity markers: chuA, yjaA and a DNA anonymous fragment designated TSPE4.C2. Were analyzed 152 samples from different origins: urine (70), feces (35), small intestine (35) and tissues (12). The mPCR was carried out to pathotypification of the isolates and a PCR with the pathogenicity markers, to classify in phylogenetic groups. Seventy seven (51%) isolates when tested by mPCR were positive to at least one of the virulence factors, while the phylogenetic characterization of the isolates showed 21 (14%) isolates in A, 65 (42%) in B1, 19 (13%) in B2 and 47 (31%) in D group. Fourteen (20%) urine isolates were characterized as UPEC; nine (12.8%) presented UPEC and ETEC factors simultaneously and four (5.8%) were classified as ETEC. In the phylogenetic classification, the groups of major occurrence were D (45.8%) and B1 (32.8%). Of the analyzed feces samples, 25 (71.4%) demonstrated virulence factors characteristic of pathotype ETEC. Phylogeneticaly, the group of higher incidence was B1 with 21 (60%) samples, followed by B2 with six (17.2%), group A with five (14.2%) and group D with three (8.6%) isolates. For the small intestine samples, 20 (57.2%) were characterized as ETEC. By the phylogeny, 23 (65.6%) isolates were classified in groups A or B1, among them 51.4% belonged to B1 and six (17.2%) were located in equal percentage in groups B2 and D. Six tissue isolates (50%) were qualified as ETEC. In regards to the phylogenetic classification, six (50%) samples were located in group D, followed by A and B1, with three (50%) samples in each group. For the tested isolates, the phylogenetic analysis demonstrated to be efficient, and it may be included in laboratorial routine for epidemiologic studies of the intestinal and extra intestinal swine samples, allowing the differentiation of pathogenic and non-pathogenic isolates, as well as helping in the strains selection for the vaccine production. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-02-18 |
dc.date.accessioned.fl_str_mv |
2017-06-13 |
dc.date.available.fl_str_mv |
2017-06-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
KOLLING, Lilian. Phylogenetic classification and pathotype characterization of pathogenic and commensal Escherichia coli isolated of swine from Southern Brazil. 2009. 55 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2009. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/10038 |
identifier_str_mv |
KOLLING, Lilian. Phylogenetic classification and pathotype characterization of pathogenic and commensal Escherichia coli isolated of swine from Southern Brazil. 2009. 55 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2009. |
url |
http://repositorio.ufsm.br/handle/1/10038 |
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por |
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por |
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500500000007 |
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Universidade Federal de Santa Maria |
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Programa de Pós-Graduação em Medicina Veterinária |
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UFSM |
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BR |
dc.publisher.department.fl_str_mv |
Medicina Veterinária |
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Universidade Federal de Santa Maria |
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