Conversão biotecnológica de casca de arroz em celulose bacteriana
Ano de defesa: | 2019 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Química
|
Departamento: |
Química
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/29838 |
Resumo: | The state of Rio Grande do Sul is the major national rice producer and generates each year large volumes of rice husk (RH) ̶ a left-over biomass with no controlled destination, composed essentially by lignin, cellulose and hemicellulose, and making up to 20% of the initial volume of the whole product. As a lignocellulosic biomass, RH can actually be converted in to biotechnological products with high added value, among them, bacterial cellulose (BC), a technological polymer with a plethora of applications (medical, food, material sciences). Aiming to yield BC, the pretreated RH (0,297 mm, washed) was submitted to alkaline hydrolysis using 6.5% NaOH solution w v-1, 35 min reaction and 80 °C. After this, a CCRD was set to verify which experiment would led to the highest concentration of fermentable sugars in hydrolysate. The experiments were carried out with dilute H2SO4 and HCl solutions, bring about as best condition 3.7% H2SO4 solution, 40 min reaction and 160 °C, ensuing a sugar production of 19,64 g glucose L-1. After that, the hydrolysate was treated by passing through activated carbon to eliminate interferers formed during the hydrolysis. The treated hydrolysate was then analyzed by HPLC-RID and concentrations up to 24.0 g glucose L-1 were obtained. This treated hydrolysate was used as fermentative medium for the production of CB by Gluconacetobacter hansenii (ATCC 23769). Preliminary tests using ozone as hydrolysate detoxificant indicate absence of sugar degradation during the ozonation, as well as no influence over the sugar consumption during the fermentation stage, which allows anticipating a good chance using it. Pronal®, yeast extract, ammonium sulfate and urea were tested as nitrogen sources (from 2 to 6 g L-1) and the best results obtained after a multivariate fermentation trial indicate the yeast extract and urea as the ultimate sources for bacterial cultivation. In the end stage of characterization of the extracted product, techniques such as FTIR, XRD and AFM were employed. Finally, a brief economic assessment was proposed; together with the good results obtained, it can summarized that RH can be looked as a viable biomass to be converted in CB ̶ fitting well within the contemporary biorefinery concept. |
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2023-08-03T15:42:16Z2023-08-03T15:42:16Z2019-01-25http://repositorio.ufsm.br/handle/1/29838The state of Rio Grande do Sul is the major national rice producer and generates each year large volumes of rice husk (RH) ̶ a left-over biomass with no controlled destination, composed essentially by lignin, cellulose and hemicellulose, and making up to 20% of the initial volume of the whole product. As a lignocellulosic biomass, RH can actually be converted in to biotechnological products with high added value, among them, bacterial cellulose (BC), a technological polymer with a plethora of applications (medical, food, material sciences). Aiming to yield BC, the pretreated RH (0,297 mm, washed) was submitted to alkaline hydrolysis using 6.5% NaOH solution w v-1, 35 min reaction and 80 °C. After this, a CCRD was set to verify which experiment would led to the highest concentration of fermentable sugars in hydrolysate. The experiments were carried out with dilute H2SO4 and HCl solutions, bring about as best condition 3.7% H2SO4 solution, 40 min reaction and 160 °C, ensuing a sugar production of 19,64 g glucose L-1. After that, the hydrolysate was treated by passing through activated carbon to eliminate interferers formed during the hydrolysis. The treated hydrolysate was then analyzed by HPLC-RID and concentrations up to 24.0 g glucose L-1 were obtained. This treated hydrolysate was used as fermentative medium for the production of CB by Gluconacetobacter hansenii (ATCC 23769). Preliminary tests using ozone as hydrolysate detoxificant indicate absence of sugar degradation during the ozonation, as well as no influence over the sugar consumption during the fermentation stage, which allows anticipating a good chance using it. Pronal®, yeast extract, ammonium sulfate and urea were tested as nitrogen sources (from 2 to 6 g L-1) and the best results obtained after a multivariate fermentation trial indicate the yeast extract and urea as the ultimate sources for bacterial cultivation. In the end stage of characterization of the extracted product, techniques such as FTIR, XRD and AFM were employed. Finally, a brief economic assessment was proposed; together with the good results obtained, it can summarized that RH can be looked as a viable biomass to be converted in CB ̶ fitting well within the contemporary biorefinery concept.O estado do Rio Grande do Sul é o maior produtor nacional de arroz, gerando grandes volumes de casca de arroz (CA), uma biomassa composta por lignina, celulose e hemicelulose, que não tem destino adequado, corresponde a 20% do volume inicial. Como biomassa lignocelulósica, a CA tem potencial para ser convertida em produtos biotecnológicos com valor econômico agregado, entre eles, celulose bacteriana (CB), polímero que conta com inúmeras aplicações (medicinal, alimentos, novos materiais). Com este objetivo, a CA pré-tratada (cominuída à 0,297 mm, lavada), foi submetida à hidrólise alcalina com solução de NaOH 6,5 % m v-1 por 35 min, a 80 °C. Após isto, foi aplicado DCCR para verificar a melhor condição que resultaria na maior concentração de açúcares fermentescíveis no hidrolisado. Os experimentos foram feitos com solução diluída de H2SO4 e HCl, obtendo-se como condição ótima 40 min de processo, 160° C e solução de H2SO4 3,7%, resultando produção de açúcares de 19,64 g glicose L-1. Após isto, o hidrolisado foi tratado com carbono ativado para eliminação de interferentes formados no processo. O hidrolisado final foi analisado por HPLC-RID, obtendo-se concentração máxima de 24,0 g glicose L-1. Este hidrolisado foi usado como meio fermentativo para a produção CB com Gluconacetobacter hansenii (ATCC 23769). Ensaios preliminares usando ozonização como técnica de destoxificação do hidrolisado indicaram ausência de degradação dos açúcares durante o processo, bem como consumo dos açúcares durante a etapa fermentativa, o que atesta a possibilidade de uso do ozônio como destoxificante. Foram testados Pronal®, extrato de levedura, sulfato de amônio e uréia como fontes de nitrogênio (2 a 6 g L-1), e os melhores resultados obtidos por testes multivariados de fermentação indicam que o extrato de levedura e a uréia são as melhores fontes para o cultivo bacteriano. Determinou-se multivariadamente que 5% de extrato de levedura é a melhor suplementação para o meio de cultivo. A caracterização final do produto extraído foi feita com o emprego de técnicas como FTIR, XRD e AFM. Ao final, breve balanço econômico foi formulado, que, juntamente com os bons resultados, comprovam a viabilidade do emprego da biomassa CA na bioconversão à CB ̶ o que se insere bem no contexto contemporâneo de biorefinaria.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em QuímicaUFSMBrasilQuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessCasca de arrozHidróliseFermentaçãoGluconacetobacter hanseniiRice huskHydrolysisFermentationCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICAConversão biotecnológica de casca de arroz em celulose bacterianaBiotechnological conversion of rice husk in bacterial celluloseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMartins, Ayrton Figueiredohttp://lattes.cnpq.br/2113532494494821Bizzi, Cezar AugustoBevilaqua, Daiane Balconihttp://lattes.cnpq.br/5869599826067118Araujo, Raiara Boessio100600000000600600600600600ade6000d-a348-4d49-a531-6500a65a153ae7ef21ac-bb99-4474-8bae-558304cb04e7be6876cc-2db6-4d50-8c79-b4b0a2425b0cd81874e3-24bb-48ea-87cb-358fb7494522reponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGQUÍMICA_2019_ARAUJO_RAIARA.pdfDIS_PPGQUÍMICA_2019_ARAUJO_RAIARA.pdfDissertação de mestradoapplication/pdf3594871http://repositorio.ufsm.br/bitstream/1/29838/1/DIS_PPGQU%c3%8dMICA_2019_ARAUJO_RAIARA.pdffec1ca3457dbbd672c56426087d05cb2MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/29838/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/29838/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD531/298382023-08-03 12:42:16.949oai:repositorio.ufsm.br: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 Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-08-03T15:42:16Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Conversão biotecnológica de casca de arroz em celulose bacteriana |
dc.title.alternative.eng.fl_str_mv |
Biotechnological conversion of rice husk in bacterial cellulose |
title |
Conversão biotecnológica de casca de arroz em celulose bacteriana |
spellingShingle |
Conversão biotecnológica de casca de arroz em celulose bacteriana Araujo, Raiara Boessio Casca de arroz Hidrólise Fermentação Gluconacetobacter hansenii Rice husk Hydrolysis Fermentation CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Conversão biotecnológica de casca de arroz em celulose bacteriana |
title_full |
Conversão biotecnológica de casca de arroz em celulose bacteriana |
title_fullStr |
Conversão biotecnológica de casca de arroz em celulose bacteriana |
title_full_unstemmed |
Conversão biotecnológica de casca de arroz em celulose bacteriana |
title_sort |
Conversão biotecnológica de casca de arroz em celulose bacteriana |
author |
Araujo, Raiara Boessio |
author_facet |
Araujo, Raiara Boessio |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Martins, Ayrton Figueiredo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2113532494494821 |
dc.contributor.referee1.fl_str_mv |
Bizzi, Cezar Augusto |
dc.contributor.referee2.fl_str_mv |
Bevilaqua, Daiane Balconi |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5869599826067118 |
dc.contributor.author.fl_str_mv |
Araujo, Raiara Boessio |
contributor_str_mv |
Martins, Ayrton Figueiredo Bizzi, Cezar Augusto Bevilaqua, Daiane Balconi |
dc.subject.por.fl_str_mv |
Casca de arroz Hidrólise Fermentação Gluconacetobacter hansenii |
topic |
Casca de arroz Hidrólise Fermentação Gluconacetobacter hansenii Rice husk Hydrolysis Fermentation CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Rice husk Hydrolysis Fermentation |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
The state of Rio Grande do Sul is the major national rice producer and generates each year large volumes of rice husk (RH) ̶ a left-over biomass with no controlled destination, composed essentially by lignin, cellulose and hemicellulose, and making up to 20% of the initial volume of the whole product. As a lignocellulosic biomass, RH can actually be converted in to biotechnological products with high added value, among them, bacterial cellulose (BC), a technological polymer with a plethora of applications (medical, food, material sciences). Aiming to yield BC, the pretreated RH (0,297 mm, washed) was submitted to alkaline hydrolysis using 6.5% NaOH solution w v-1, 35 min reaction and 80 °C. After this, a CCRD was set to verify which experiment would led to the highest concentration of fermentable sugars in hydrolysate. The experiments were carried out with dilute H2SO4 and HCl solutions, bring about as best condition 3.7% H2SO4 solution, 40 min reaction and 160 °C, ensuing a sugar production of 19,64 g glucose L-1. After that, the hydrolysate was treated by passing through activated carbon to eliminate interferers formed during the hydrolysis. The treated hydrolysate was then analyzed by HPLC-RID and concentrations up to 24.0 g glucose L-1 were obtained. This treated hydrolysate was used as fermentative medium for the production of CB by Gluconacetobacter hansenii (ATCC 23769). Preliminary tests using ozone as hydrolysate detoxificant indicate absence of sugar degradation during the ozonation, as well as no influence over the sugar consumption during the fermentation stage, which allows anticipating a good chance using it. Pronal®, yeast extract, ammonium sulfate and urea were tested as nitrogen sources (from 2 to 6 g L-1) and the best results obtained after a multivariate fermentation trial indicate the yeast extract and urea as the ultimate sources for bacterial cultivation. In the end stage of characterization of the extracted product, techniques such as FTIR, XRD and AFM were employed. Finally, a brief economic assessment was proposed; together with the good results obtained, it can summarized that RH can be looked as a viable biomass to be converted in CB ̶ fitting well within the contemporary biorefinery concept. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019-01-25 |
dc.date.accessioned.fl_str_mv |
2023-08-03T15:42:16Z |
dc.date.available.fl_str_mv |
2023-08-03T15:42:16Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://repositorio.ufsm.br/handle/1/29838 |
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http://repositorio.ufsm.br/handle/1/29838 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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100600000000 |
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600 600 600 600 600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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Programa de Pós-Graduação em Química |
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UFSM |
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Brasil |
dc.publisher.department.fl_str_mv |
Química |
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Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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