Otimização na produção do concentrado proteico de farelo de arroz e utilização na alimentação do jundiá (Rhamdia quelen)

Detalhes bibliográficos
Ano de defesa: 2020
Autor(a) principal: Loureiro, Bruno Bianch
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
dARK ID: ark:/26339/0013000001jf3
Idioma: por
Instituição de defesa: Universidade Federal de Santa Maria
Brasil
Zootecnia
UFSM
Programa de Pós-Graduação em Zootecnia
Centro de Ciências Rurais
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://repositorio.ufsm.br/handle/1/21178
Resumo: The present study was carried out with the objective of evaluating the effects of the inclusion of de-oiled and de-phytinized rice bran protein concentrate (DRBPC) on the parameters of growth and performance, metabolic responses (serum and liver), nutrient deposition and digestive enzymes of silver catfish (Rhamdia quelen), fed with diets containing different levels of replacement of protein from fishmeal by DRBPC. For the development and choice of the best DRBPC, obtained from de-oiled and dephytinized rice bran (DDRB), the influence of the DDRB extrusion process on the improvement of the extraction and concentration of the fresh and extruded DDRB protein was tested, obtaining two protein concentrates: DRBPC and DRBPCext. The results obtained indicated that the extrusion process per se or combined with the concentration methodology was not efficient to increase the crude protein (CP) content of the DDRB, presenting lower values (P <0.05) compared to protein concentrate from DRBPC. After obtaining and choosing the concentrate with the highest CP content (42.39%), DRBPC protein was included in different levels (25, 35, 45 and 55%) of fishmeal replacement in silver catfish diets. A biological assay was conducted during 45 experimental days, where 300 silver catfish, with initial body weight 10.28 ± 0.19 g were randomly distributed and allocated in 15 polypropylene tanks (20 fish per tank) with a volume of 100 L connected to a thermo regulated water recirculation system. The fish were fed with experimental diets, three times a day (9 am, 1:30 pm and 5 pm), until apparent satiety. The experimental design was completely randomized with five treatments and three replications. The data were submitted to analysis of variance (ANOVA) and the means compared to the Tukey test (p <0.05). At the end of the experimental period, a decrease in performance was observed (P <0.05) on weight gain, specific growth rate and relative weight gain in fish fed diets containing 45 and 55% of DRBPC inclusion. For total protein deposited, a reduction (P <0.05) was verified in fish that received diets containing 35% or more of the inclusion of DRBPC. The highest activity of the digestive enzyme trypsin was observed in fish fed diet 35% of DRBPC. Based on the results obtained, it was found that the DDRB extrusion process was not efficient to facilitate the extraction of the bran protein by the protein concentration process. The protein concentration carried out from the DDRB in natura, allowed to obtain a concentrate with a higher crude protein content (42.39%). As for the results of the biological test, it was observed that the inclusion of 25% of the DRBPC in replacement of the protein coming from fishmeal did not negatively affect the parameters of growth and metabolic, deposition of nutrients and digestive enzymes of silver catfish (Rhamdia quelen).
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spelling Otimização na produção do concentrado proteico de farelo de arroz e utilização na alimentação do jundiá (Rhamdia quelen)Rice meal residue as a protein ingredient in the feeding of silver catfish (Rhamdia quelen)ExtrusãoProteínaFarelo de arroz desengordurado e desfitinizadoEnzimas digestivasExtrusionProteinDe-oiled and de-phytinized rice branDigestive enzymesCNPQ::CIENCIAS AGRARIAS::ZOOTECNIAThe present study was carried out with the objective of evaluating the effects of the inclusion of de-oiled and de-phytinized rice bran protein concentrate (DRBPC) on the parameters of growth and performance, metabolic responses (serum and liver), nutrient deposition and digestive enzymes of silver catfish (Rhamdia quelen), fed with diets containing different levels of replacement of protein from fishmeal by DRBPC. For the development and choice of the best DRBPC, obtained from de-oiled and dephytinized rice bran (DDRB), the influence of the DDRB extrusion process on the improvement of the extraction and concentration of the fresh and extruded DDRB protein was tested, obtaining two protein concentrates: DRBPC and DRBPCext. The results obtained indicated that the extrusion process per se or combined with the concentration methodology was not efficient to increase the crude protein (CP) content of the DDRB, presenting lower values (P <0.05) compared to protein concentrate from DRBPC. After obtaining and choosing the concentrate with the highest CP content (42.39%), DRBPC protein was included in different levels (25, 35, 45 and 55%) of fishmeal replacement in silver catfish diets. A biological assay was conducted during 45 experimental days, where 300 silver catfish, with initial body weight 10.28 ± 0.19 g were randomly distributed and allocated in 15 polypropylene tanks (20 fish per tank) with a volume of 100 L connected to a thermo regulated water recirculation system. The fish were fed with experimental diets, three times a day (9 am, 1:30 pm and 5 pm), until apparent satiety. The experimental design was completely randomized with five treatments and three replications. The data were submitted to analysis of variance (ANOVA) and the means compared to the Tukey test (p <0.05). At the end of the experimental period, a decrease in performance was observed (P <0.05) on weight gain, specific growth rate and relative weight gain in fish fed diets containing 45 and 55% of DRBPC inclusion. For total protein deposited, a reduction (P <0.05) was verified in fish that received diets containing 35% or more of the inclusion of DRBPC. The highest activity of the digestive enzyme trypsin was observed in fish fed diet 35% of DRBPC. Based on the results obtained, it was found that the DDRB extrusion process was not efficient to facilitate the extraction of the bran protein by the protein concentration process. The protein concentration carried out from the DDRB in natura, allowed to obtain a concentrate with a higher crude protein content (42.39%). As for the results of the biological test, it was observed that the inclusion of 25% of the DRBPC in replacement of the protein coming from fishmeal did not negatively affect the parameters of growth and metabolic, deposition of nutrients and digestive enzymes of silver catfish (Rhamdia quelen).O presente estudo foi realizado com o objetivo de avaliar os efeitos da inclusão do concentrado proteico de farelo de arroz (CPFA) sobre os parâmetros de crescimento, respostas metabólicas (plasmáticas e hepáticas), deposição de nutrientes e enzimas digestivas de jundiás (Rhamdia quelen): em substituição a farinha de peixe. Para o desenvolvimento e escolha do melhor processo para obtenção do CPFA a partir do farelo de arroz desengordurado e desfitinizado (FADD), foi testada a influência do processo de extrusão do FADD sobre a extração e concentração da proteína do FADD in natura e extrusado. Os resultados obtidos indicaram que o processo de extrusão per se ou combinado com a metodologia de concentração, não foi eficiente para elevar o teor de proteína bruta (PB) do farelo in natura, apresentando valores inferiores (P<0,05) em comparação ao concentrado proteico obtido a partir da FADD in natura. Após a obtenção e escolha do concentrado com maior teor de PB (42,39%), a proteína do CPFA foi inclusa em diferentes níveis (25, 35, 45 e 55%) em substiuição a proteína da farinha de peixe, nas dietas para jundiás. Um ensaio biológico foi conduzido durante 45 dias experimentais, onde 300 jundiás, com peso médio inicial 10,28 ± 0,19 g foram distribuídos aleatoriamente e alocados em 15 tanques de polipropileno (20 peixes por tanque) com volume de 100 L conectados a um sistema de recirculação de água termorregulado. Os peixes foram alimentados com as dietas experimentais, três vezes ao dia (9h, 13h30min e 17h), até a saciedade aparente. O delineamento experimental foi inteiramente casualizado com cinco tratamentos e três repetições. Os dados foram submetidos à análise de variância (ANOVA) e as médias comparadas ao teste de Tukey (P<0,05). Ao final do período experimental, foram observadas queda no desempenho (P<0,05) sobre os parâmetros de ganho de peso, taxa de crescimento específico e ganho de peso relativo nos peixes alimentados com as dietas contendo 45 e 55% de inclusão do CPFA. Para proteína total depositada, foi verificada a redução (P<0,05) nos peixes que receberam as dietas contendo 35% ou mais da inclusão do CPFA. A maior atividade da enzima digestiva tripsina foi observada nos peixes alimentados com as dietas 35% de CPFA. Não foram observadas diferenças (P<0,05) para as respostas metabólicas plasmáticas e hepáticas. A concentração proteica realizada a partir do FADD in natura, permitiu a obtenção de um concentrado com maior teor de proteína bruta (42,39%). Quanto aos resultados do ensaio biológico, foi observado que a inclusão de até 35% do CPFA em substituição a proteína advinda da farinha de peixe não afeta negativamente os parâmetros de crescimento, metabólicos, deposição de nutrientes e enzimas digestivas de jundiás (Rhamdia quelen)Universidade Federal de Santa MariaBrasilZootecniaUFSMPrograma de Pós-Graduação em ZootecniaCentro de Ciências RuraisSilva, Leila Picolli dahttp://lattes.cnpq.br/9378190351379861Pretto, AlexandraXXXXXXXXXXXXXXXFerrigolo, Fernanda Rodrigues GoulartXXXXXXXXXXXXXXXXXXLoro, Vânia LuciaXXXXXXXXXXXXXXXCosta, Silvio Teixeira daXXXXXXXXXXXXXXXXXXLoureiro, Bruno Bianch2021-06-22T17:56:02Z2021-06-22T17:56:02Z2020-03-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/21178ark:/26339/0013000001jf3porAttribution-NonCommercial-NoDerivatives 4.0 Internationalinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2021-06-23T06:03:42Zoai:repositorio.ufsm.br:1/21178Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/PUBhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.com||manancial@ufsm.bropendoar:2021-06-23T06:03:42Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Otimização na produção do concentrado proteico de farelo de arroz e utilização na alimentação do jundiá (Rhamdia quelen)
Rice meal residue as a protein ingredient in the feeding of silver catfish (Rhamdia quelen)
title Otimização na produção do concentrado proteico de farelo de arroz e utilização na alimentação do jundiá (Rhamdia quelen)
spellingShingle Otimização na produção do concentrado proteico de farelo de arroz e utilização na alimentação do jundiá (Rhamdia quelen)
Loureiro, Bruno Bianch
Extrusão
Proteína
Farelo de arroz desengordurado e desfitinizado
Enzimas digestivas
Extrusion
Protein
De-oiled and de-phytinized rice bran
Digestive enzymes
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA
title_short Otimização na produção do concentrado proteico de farelo de arroz e utilização na alimentação do jundiá (Rhamdia quelen)
title_full Otimização na produção do concentrado proteico de farelo de arroz e utilização na alimentação do jundiá (Rhamdia quelen)
title_fullStr Otimização na produção do concentrado proteico de farelo de arroz e utilização na alimentação do jundiá (Rhamdia quelen)
title_full_unstemmed Otimização na produção do concentrado proteico de farelo de arroz e utilização na alimentação do jundiá (Rhamdia quelen)
title_sort Otimização na produção do concentrado proteico de farelo de arroz e utilização na alimentação do jundiá (Rhamdia quelen)
author Loureiro, Bruno Bianch
author_facet Loureiro, Bruno Bianch
author_role author
dc.contributor.none.fl_str_mv Silva, Leila Picolli da
http://lattes.cnpq.br/9378190351379861
Pretto, Alexandra
XXXXXXXXXXXXXXX
Ferrigolo, Fernanda Rodrigues Goulart
XXXXXXXXXXXXXXXXXX
Loro, Vânia Lucia
XXXXXXXXXXXXXXX
Costa, Silvio Teixeira da
XXXXXXXXXXXXXXXXXX
dc.contributor.author.fl_str_mv Loureiro, Bruno Bianch
dc.subject.por.fl_str_mv Extrusão
Proteína
Farelo de arroz desengordurado e desfitinizado
Enzimas digestivas
Extrusion
Protein
De-oiled and de-phytinized rice bran
Digestive enzymes
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA
topic Extrusão
Proteína
Farelo de arroz desengordurado e desfitinizado
Enzimas digestivas
Extrusion
Protein
De-oiled and de-phytinized rice bran
Digestive enzymes
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA
description The present study was carried out with the objective of evaluating the effects of the inclusion of de-oiled and de-phytinized rice bran protein concentrate (DRBPC) on the parameters of growth and performance, metabolic responses (serum and liver), nutrient deposition and digestive enzymes of silver catfish (Rhamdia quelen), fed with diets containing different levels of replacement of protein from fishmeal by DRBPC. For the development and choice of the best DRBPC, obtained from de-oiled and dephytinized rice bran (DDRB), the influence of the DDRB extrusion process on the improvement of the extraction and concentration of the fresh and extruded DDRB protein was tested, obtaining two protein concentrates: DRBPC and DRBPCext. The results obtained indicated that the extrusion process per se or combined with the concentration methodology was not efficient to increase the crude protein (CP) content of the DDRB, presenting lower values (P <0.05) compared to protein concentrate from DRBPC. After obtaining and choosing the concentrate with the highest CP content (42.39%), DRBPC protein was included in different levels (25, 35, 45 and 55%) of fishmeal replacement in silver catfish diets. A biological assay was conducted during 45 experimental days, where 300 silver catfish, with initial body weight 10.28 ± 0.19 g were randomly distributed and allocated in 15 polypropylene tanks (20 fish per tank) with a volume of 100 L connected to a thermo regulated water recirculation system. The fish were fed with experimental diets, three times a day (9 am, 1:30 pm and 5 pm), until apparent satiety. The experimental design was completely randomized with five treatments and three replications. The data were submitted to analysis of variance (ANOVA) and the means compared to the Tukey test (p <0.05). At the end of the experimental period, a decrease in performance was observed (P <0.05) on weight gain, specific growth rate and relative weight gain in fish fed diets containing 45 and 55% of DRBPC inclusion. For total protein deposited, a reduction (P <0.05) was verified in fish that received diets containing 35% or more of the inclusion of DRBPC. The highest activity of the digestive enzyme trypsin was observed in fish fed diet 35% of DRBPC. Based on the results obtained, it was found that the DDRB extrusion process was not efficient to facilitate the extraction of the bran protein by the protein concentration process. The protein concentration carried out from the DDRB in natura, allowed to obtain a concentrate with a higher crude protein content (42.39%). As for the results of the biological test, it was observed that the inclusion of 25% of the DRBPC in replacement of the protein coming from fishmeal did not negatively affect the parameters of growth and metabolic, deposition of nutrients and digestive enzymes of silver catfish (Rhamdia quelen).
publishDate 2020
dc.date.none.fl_str_mv 2020-03-27
2021-06-22T17:56:02Z
2021-06-22T17:56:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/21178
dc.identifier.dark.fl_str_mv ark:/26339/0013000001jf3
url http://repositorio.ufsm.br/handle/1/21178
identifier_str_mv ark:/26339/0013000001jf3
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Zootecnia
UFSM
Programa de Pós-Graduação em Zootecnia
Centro de Ciências Rurais
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Zootecnia
UFSM
Programa de Pós-Graduação em Zootecnia
Centro de Ciências Rurais
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com||manancial@ufsm.br
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