Abordagens integrativas na prevenção de recaída por morfina em ratos: organoterápico e isoterápico

Detalhes bibliográficos
Ano de defesa: 2024
Autor(a) principal: Fontoura, Murilo Barboza
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Santa Maria
Brasil
Farmacologia
UFSM
Programa de Pós-Graduação em Farmacologia
Centro de Ciências da Saúde
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://repositorio.ufsm.br/handle/1/31580
Resumo: The World Health Organization (WHO) determines drug addiction as a chronic and recurrent disease, constituting an alarming public health problem. Considering the recreational use of psychoactive drugs, such as morphine (MORPH), dependence (compulsion to use), tolerance, abstinence syndrome and relapse can be triggered after withdrawal. Reinstatement to opioid drug use is one of the main obstacles to the success of detoxification treatment, since most individuals fall back to drug use after the detoxification period. In general, the pharmacological treatments developed to date are only symptomatic, proving to be ineffective when the situation involves drug addiction. In this sense, natural therapies such as ultra-diluted medicines that aim to reestablish and integrate the organism into its homeostasis represent promising therapeutic potential in the treatment of this condition, since the dysregulation of the homeostatic balance is directly related to impulsive-compulsive, emotional, social and physical symptoms caused by the drug. This project aims to evaluate the therapeutic potential of both medicines on behavioral and molecular parameters related to MORPH reinstatement. Rats were exposed to MORPH in the conditioned place preference (CPP) paradigm to assess the drug's addictive and drug-seeking symptoms, and subsequently treated with both homeopathy medicines for 14 days. After MORPH-preference extinction, animals were challenged to MORPH reconditioning in the same CPP paradigm to evaluate MOPRH-reinstatement. Behaviors related to drug addiction, anxiety-like, memory and locomotion were assessed evaluated during drug withdrawal, followed by ex vivo assays. Our results showed MORPH-preference confirming the strong hedonic power of the drug. In the memory test and locomotor activity, no differences were observed between the groups, thus confirming the results of the CPP paradigm. Both ISO and ORG medicines prevented reinstatement-like behavior of MORPH. Molecular analysis are still being carried out. In conclusion, as far as we know, this study shows by the first time that both ultra-high dilution medicines may be an alternative which can contribute with opioid addiction reestablishing the body's physiological functions when exposed to toxic agents such as MORPH.
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spelling Abordagens integrativas na prevenção de recaída por morfina em ratos: organoterápico e isoterápicoIntegrative health practices on morphine-reinstatement in rats: organotherapic and isotherapicOpioidesDrogas psicoativasTerapias naturaisParadigma de preferência de lugar condicionado (PLC)Opioid addictionPsychoactive drugsNatural therapiesConditioned place preference (CPP)CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIAThe World Health Organization (WHO) determines drug addiction as a chronic and recurrent disease, constituting an alarming public health problem. Considering the recreational use of psychoactive drugs, such as morphine (MORPH), dependence (compulsion to use), tolerance, abstinence syndrome and relapse can be triggered after withdrawal. Reinstatement to opioid drug use is one of the main obstacles to the success of detoxification treatment, since most individuals fall back to drug use after the detoxification period. In general, the pharmacological treatments developed to date are only symptomatic, proving to be ineffective when the situation involves drug addiction. In this sense, natural therapies such as ultra-diluted medicines that aim to reestablish and integrate the organism into its homeostasis represent promising therapeutic potential in the treatment of this condition, since the dysregulation of the homeostatic balance is directly related to impulsive-compulsive, emotional, social and physical symptoms caused by the drug. This project aims to evaluate the therapeutic potential of both medicines on behavioral and molecular parameters related to MORPH reinstatement. Rats were exposed to MORPH in the conditioned place preference (CPP) paradigm to assess the drug's addictive and drug-seeking symptoms, and subsequently treated with both homeopathy medicines for 14 days. After MORPH-preference extinction, animals were challenged to MORPH reconditioning in the same CPP paradigm to evaluate MOPRH-reinstatement. Behaviors related to drug addiction, anxiety-like, memory and locomotion were assessed evaluated during drug withdrawal, followed by ex vivo assays. Our results showed MORPH-preference confirming the strong hedonic power of the drug. In the memory test and locomotor activity, no differences were observed between the groups, thus confirming the results of the CPP paradigm. Both ISO and ORG medicines prevented reinstatement-like behavior of MORPH. Molecular analysis are still being carried out. In conclusion, as far as we know, this study shows by the first time that both ultra-high dilution medicines may be an alternative which can contribute with opioid addiction reestablishing the body's physiological functions when exposed to toxic agents such as MORPH.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESDe acordo com a Organização Mundial de Saúde, o uso indiscriminado e recreacional de drogas psicoativas, especialmente opioides, têm causado um grave problema de saúde pública em nível mundial, e caracterizando a reconhecida crise dos opioides nos E.U.A. A drogadição caracteriza-se pela presença de fatores, dentre os quais, dependência (compulsão ao uso), tolerância, sintomas de abstinência na retirada e recaída que podem ser desencadeados após algumas exposições à droga. A recaída ao uso destas substâncias mostra-se como o principal obstáculo para o sucesso do tratamento de desintoxicação, uma vez que a maior parte dos indivíduos recorre ao uso da mesma após o período de detoxificação. De um modo geral, as abordagens farmacológicas utilizadas até o momento têm sido apenas sintomáticas, mostrando-se pouco eficazes quando a situação envolve drogadição. Nesse sentido, a busca por terapias integrativas, e entre estas, os tratamentos com medicamentos homeopáticos podem representar um avanço para o reestabelecimento da homeostase orgânica, uma vez que a desregulação desta homeostase se relaciona diretamente aos sintomas impulsivo-compulsivos, emocionais, sociais e físicos desenvolvidos pela drogadição. O presente estudo tem por objetivo avaliar o potencial terapêutico de duas preparações homeopáticas, ou seja, um isoterápico de morfina (ISO) e um organoterápico obtido a partir do cérebro total de carneiro saudável (ORG), sobre parâmetros comportamentais e moleculares consequentes à recaída por morfina em ratos. Para isso, os animais foram expostos à morfina no paradigma de preferência de lugar condicionado (PLC) para a indução inicial de comportamentos de preferência e busca pela droga, com subsequente tratamento com ISO ou ORG durante 14 dias. Após a observação da extinção da preferência pela morfina no paradigma de PLC, os animais foram desafiados a comportamento de recaída à preferência pela droga no mesmo aparato de PLC. Como esperado, os animais expostos à morfina apresentaram comportamento de busca pela droga, confirmando o forte poder hedônico da droga, o quais não mostraram prejuízos de memória, locomoção ou de ansiedade, confirmando que a busca pela droga foi por impulso hedônico. Ambos os medicamentos ISO e ORG foram capazes de prevenir a recaída pela morfina. Avaliações em nível molecular envolvendo o sistema dopaminérgico, glutamatérgico e opioide devem ser desenvolvidos na sequência desta dissertação, na busca de mecanismos relacionados aos benefícios comportamentais aqui observados. Em conclusão, até onde sabemos, este estudo mostra pela primeira vez que ambos medicamentos homeopáticos aqui estudados representam uma prática integrativa que pode ser útil no tratamento da drogadiçao por opioides, contribuindo para o reestabelecimento da homeostase orgânica comprometida pelo uso crônico da droga.Universidade Federal de Santa MariaBrasilFarmacologiaUFSMPrograma de Pós-Graduação em FarmacologiaCentro de Ciências da SaúdeBurger, Marilise Escobarhttp://lattes.cnpq.br/9128090974948413Segat, Hecson JesserRubin, Maribel AntonelloFontoura, Murilo Barboza2024-02-27T14:15:33Z2024-02-27T14:15:33Z2024-01-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/31580porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2024-02-27T14:15:33Zoai:repositorio.ufsm.br:1/31580Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/PUBhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.com||manancial@ufsm.bropendoar:2024-02-27T14:15:33Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Abordagens integrativas na prevenção de recaída por morfina em ratos: organoterápico e isoterápico
Integrative health practices on morphine-reinstatement in rats: organotherapic and isotherapic
title Abordagens integrativas na prevenção de recaída por morfina em ratos: organoterápico e isoterápico
spellingShingle Abordagens integrativas na prevenção de recaída por morfina em ratos: organoterápico e isoterápico
Fontoura, Murilo Barboza
Opioides
Drogas psicoativas
Terapias naturais
Paradigma de preferência de lugar condicionado (PLC)
Opioid addiction
Psychoactive drugs
Natural therapies
Conditioned place preference (CPP)
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
title_short Abordagens integrativas na prevenção de recaída por morfina em ratos: organoterápico e isoterápico
title_full Abordagens integrativas na prevenção de recaída por morfina em ratos: organoterápico e isoterápico
title_fullStr Abordagens integrativas na prevenção de recaída por morfina em ratos: organoterápico e isoterápico
title_full_unstemmed Abordagens integrativas na prevenção de recaída por morfina em ratos: organoterápico e isoterápico
title_sort Abordagens integrativas na prevenção de recaída por morfina em ratos: organoterápico e isoterápico
author Fontoura, Murilo Barboza
author_facet Fontoura, Murilo Barboza
author_role author
dc.contributor.none.fl_str_mv Burger, Marilise Escobar
http://lattes.cnpq.br/9128090974948413
Segat, Hecson Jesser
Rubin, Maribel Antonello
dc.contributor.author.fl_str_mv Fontoura, Murilo Barboza
dc.subject.por.fl_str_mv Opioides
Drogas psicoativas
Terapias naturais
Paradigma de preferência de lugar condicionado (PLC)
Opioid addiction
Psychoactive drugs
Natural therapies
Conditioned place preference (CPP)
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
topic Opioides
Drogas psicoativas
Terapias naturais
Paradigma de preferência de lugar condicionado (PLC)
Opioid addiction
Psychoactive drugs
Natural therapies
Conditioned place preference (CPP)
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
description The World Health Organization (WHO) determines drug addiction as a chronic and recurrent disease, constituting an alarming public health problem. Considering the recreational use of psychoactive drugs, such as morphine (MORPH), dependence (compulsion to use), tolerance, abstinence syndrome and relapse can be triggered after withdrawal. Reinstatement to opioid drug use is one of the main obstacles to the success of detoxification treatment, since most individuals fall back to drug use after the detoxification period. In general, the pharmacological treatments developed to date are only symptomatic, proving to be ineffective when the situation involves drug addiction. In this sense, natural therapies such as ultra-diluted medicines that aim to reestablish and integrate the organism into its homeostasis represent promising therapeutic potential in the treatment of this condition, since the dysregulation of the homeostatic balance is directly related to impulsive-compulsive, emotional, social and physical symptoms caused by the drug. This project aims to evaluate the therapeutic potential of both medicines on behavioral and molecular parameters related to MORPH reinstatement. Rats were exposed to MORPH in the conditioned place preference (CPP) paradigm to assess the drug's addictive and drug-seeking symptoms, and subsequently treated with both homeopathy medicines for 14 days. After MORPH-preference extinction, animals were challenged to MORPH reconditioning in the same CPP paradigm to evaluate MOPRH-reinstatement. Behaviors related to drug addiction, anxiety-like, memory and locomotion were assessed evaluated during drug withdrawal, followed by ex vivo assays. Our results showed MORPH-preference confirming the strong hedonic power of the drug. In the memory test and locomotor activity, no differences were observed between the groups, thus confirming the results of the CPP paradigm. Both ISO and ORG medicines prevented reinstatement-like behavior of MORPH. Molecular analysis are still being carried out. In conclusion, as far as we know, this study shows by the first time that both ultra-high dilution medicines may be an alternative which can contribute with opioid addiction reestablishing the body's physiological functions when exposed to toxic agents such as MORPH.
publishDate 2024
dc.date.none.fl_str_mv 2024-02-27T14:15:33Z
2024-02-27T14:15:33Z
2024-01-12
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/31580
url http://repositorio.ufsm.br/handle/1/31580
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Farmacologia
UFSM
Programa de Pós-Graduação em Farmacologia
Centro de Ciências da Saúde
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Farmacologia
UFSM
Programa de Pós-Graduação em Farmacologia
Centro de Ciências da Saúde
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com||manancial@ufsm.br
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