Efeito da funcionalização de lipossomas com polímero na eficiência de encapsulamento de clodronato e na estabilidade da vesícula

• Ano de defesa: 2017
• Autor(a) principal: Ventura, Poliana de Oliveira [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• dARK ID: ark:/48912/001300001xhsx
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Liposomes; Peg; Drug delivery; Incapsulation; Fluorescence; Lipossomos; Encapsulamento; Fluorescência
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=4997549; http://repositorio.unifesp.br/handle/11600/50509

Encapsulating a medicament can direct it to the targeted tissue, which enables the administration of smaller doses of a certain drug. The liposomes are safe and efficient carriers for the delivery of drugs. Although, due to their high biocompatibility, they are rapidly captured decreasing it’s delivery potential in the targeted tissue. An alternative aiming the avoidance of this effect is the functionalization of the liposomes with molecules that make the accession of immune system components and/or the nonspecific interaction with other tissues more difficult. Utilizing polymers in the surface of these carriers has resulted in an increase in their circulation time. Therefore, the manipulation of the physical-chemistry characteristics, like the kind of polymer, the size, the composition, the pH or temperature variances, of this system is important in the understanding and in the design of new drug-delivery carriers. The aim of the work was to study de physical-chemistry aspects involved in the functionalization of liposomes composed of stearoyl oleoyl phosphatidylcholine (SOPC) and polyethylene glycol 400 monolaurate (PEG400ML), which were obtained by extrusion. The vesicles stability was observed for three consecutive days through different techniques, such as dynamic light scattering measurements (DLS), monitoring their size, and zeta potential measurements, SOPC LUVs carboxyfluorescein leakage assays with different PEG400ML concentrations and comparing SOPC LUVs with cholesterol, already known and described in literature. The results obtained indicate that particles propensity increase and aggregate over the course of days. Although, the kinetic of leakage of carboxyfluorescein show that the vesicles of SOPC with 2,5 mol% of PEG400ML exhibit relatively stable during the tree days of experimente. Corroborate that the studys the measures of DSC showed that the best formulation to work and more stable for application as vehicle of drug delivery carrier has the formulation of SOPC with 2,5 mol% of PEG400ML.