Estudo da expressão das aquaporinas 1 e 2 em nervo facial de ratos após paralisia facial
| Ano de defesa: | 2018 |
|---|---|
| Autor(a) principal: | |
| Orientador(a): | |
| Banca de defesa: | |
| Tipo de documento: | Tese |
| Tipo de acesso: | Acesso aberto |
| dARK ID: | ark:/48912/001300001pgpm |
| Idioma: | por |
| Instituição de defesa: |
Universidade Federal de São Paulo (UNIFESP)
|
| Programa de Pós-Graduação: |
Não Informado pela instituição
|
| Departamento: |
Não Informado pela instituição
|
| País: |
Não Informado pela instituição
|
| Palavras-chave em Português: | |
| Link de acesso: | https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=6370236 https://repositorio.unifesp.br/handle/11600/52841 |
Resumo: | OBJECTIVE: The aim of the study was to describe the expression of aquaporins (AQPs) 1 and 2 in the extra temporal facial nerve(NF) of rats within 7 days after mechanical compression. METHODS: The extra temporal trunk of the right NF of 20 rats was mechanically crushed and after 1, 3 and 7 days, AQP2 expression was analyzed by western blot (WB) (n = 15) and AQP1 expression was analyzed by immunohistochemistry (IHC) (n = 5) in the injured (right) and non injured (left) NF. During this period, the behavioral analysis of the facial paralysis installation in the animals was also performed. RESULTS: WB demonstrated the presence and elevation of AQP2 in injured NF in relation to its control on the first day after neural compression in concomitance with the installation of facial paralysis. Using IHC, the antiAQP1 antibody’s marking occurred mainly in Schwann cells (CS) and endothelial cells. The count of CS marked by the antiAQP1 antibody was higher in the injured nerves compared to the noninjured. CONCLUSION: The concomitance of early elevation of AQP 1 and 2 expressions during the installation of facial`s nerve deficit suggests the possibility that this protein is involved in the physiological events of edema after neural crush. A better understanding of the role of AQPs in NF may lead to the development of new therapies. |
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Estudo da expressão das aquaporinas 1 e 2 em nervo facial de ratos após paralisia facialStudy of expression of aquaporins 1 and 2 following facial nerve crush in rats.Nervo facialParalisia facialAquaporina 1Aquaporina 2Western blotImunohistoquímicaOBJECTIVE: The aim of the study was to describe the expression of aquaporins (AQPs) 1 and 2 in the extra temporal facial nerve(NF) of rats within 7 days after mechanical compression. METHODS: The extra temporal trunk of the right NF of 20 rats was mechanically crushed and after 1, 3 and 7 days, AQP2 expression was analyzed by western blot (WB) (n = 15) and AQP1 expression was analyzed by immunohistochemistry (IHC) (n = 5) in the injured (right) and non injured (left) NF. During this period, the behavioral analysis of the facial paralysis installation in the animals was also performed. RESULTS: WB demonstrated the presence and elevation of AQP2 in injured NF in relation to its control on the first day after neural compression in concomitance with the installation of facial paralysis. Using IHC, the antiAQP1 antibody’s marking occurred mainly in Schwann cells (CS) and endothelial cells. The count of CS marked by the antiAQP1 antibody was higher in the injured nerves compared to the noninjured. CONCLUSION: The concomitance of early elevation of AQP 1 and 2 expressions during the installation of facial`s nerve deficit suggests the possibility that this protein is involved in the physiological events of edema after neural crush. A better understanding of the role of AQPs in NF may lead to the development of new therapies.OBJETIVO: Avaliar a expressão das aquaporinas (AQPs) 1 e 2 no nervo facial (NF) extratemporal de ratos, em até 7 dias após lesão traumática por compressão mecânica. MÉTODO: O tronco extratemporal do NF direito de 20 ratos foi esmagado mecanicamente e, após 1, 3 e 7 dias, foi analisada a expressão da AQP2 por meio da técnica de westernblot (WB) (n=15) e a expressão da AQP1 através de imunoistoquímica (IHQ) (n=5) no NF lesado (direito) e não lesado (esquerdo). Nesse período foi realizada também a análise comportamental da instalação de paralisia facial nos animais. RESULTADOS: Foi verificado por meio de WB a presença e elevação da AQP2 no NF lesado em relação ao seu controle, no primeiro dia após a compressão neural em concomitância à instalação da paralisia facial. Na IHQ, a marcação do anticorpo antiAQP1 ocorreu principalmente em células de Schwann (CS) e células endoteliais. A contagem das CS marcadas pelo anticorpo antiAQP1 foi maior nos nervos lesados em relação aos não lesados. CONCLUSÃO: A concomitância da elevação precoce das expressões de AQPs 1 e 2, durante a instalação de deficit no nervo facial de ratos, sugere a possibilidade de que essa proteína esteja envolvida nos eventos fisiológicos de edema após lesão neural por esmagamento. Uma melhor compreensão do papel das AQPs no NF pode levar ao desenvolvimento de novas terapias.Dados abertos - Sucupira - Teses e dissertações (2018)Universidade Federal de São Paulo (UNIFESP)Testa, Jose Ricardo Gurgel [UNIFESP]http://lattes.cnpq.br/1154965263654209http://lattes.cnpq.br/4384725487909697Universidade Federal de São Paulo (UNIFESP)Neiva, Felipe Costa [UNIFESP]2020-03-25T12:10:35Z2020-03-25T12:10:35Z2018-06-28info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersion56 f.application/pdfhttps://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=63702362018-0785.pdfhttps://repositorio.unifesp.br/handle/11600/52841ark:/48912/001300001pgpmporSão Pauloinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-10T14:38:29Zoai:repositorio.unifesp.br:11600/52841Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-10T14:38:29Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Estudo da expressão das aquaporinas 1 e 2 em nervo facial de ratos após paralisia facial Study of expression of aquaporins 1 and 2 following facial nerve crush in rats. |
| title |
Estudo da expressão das aquaporinas 1 e 2 em nervo facial de ratos após paralisia facial |
| spellingShingle |
Estudo da expressão das aquaporinas 1 e 2 em nervo facial de ratos após paralisia facial Neiva, Felipe Costa [UNIFESP] Nervo facial Paralisia facial Aquaporina 1 Aquaporina 2 Western blot Imunohistoquímica |
| title_short |
Estudo da expressão das aquaporinas 1 e 2 em nervo facial de ratos após paralisia facial |
| title_full |
Estudo da expressão das aquaporinas 1 e 2 em nervo facial de ratos após paralisia facial |
| title_fullStr |
Estudo da expressão das aquaporinas 1 e 2 em nervo facial de ratos após paralisia facial |
| title_full_unstemmed |
Estudo da expressão das aquaporinas 1 e 2 em nervo facial de ratos após paralisia facial |
| title_sort |
Estudo da expressão das aquaporinas 1 e 2 em nervo facial de ratos após paralisia facial |
| author |
Neiva, Felipe Costa [UNIFESP] |
| author_facet |
Neiva, Felipe Costa [UNIFESP] |
| author_role |
author |
| dc.contributor.none.fl_str_mv |
Testa, Jose Ricardo Gurgel [UNIFESP] http://lattes.cnpq.br/1154965263654209 http://lattes.cnpq.br/4384725487909697 Universidade Federal de São Paulo (UNIFESP) |
| dc.contributor.author.fl_str_mv |
Neiva, Felipe Costa [UNIFESP] |
| dc.subject.por.fl_str_mv |
Nervo facial Paralisia facial Aquaporina 1 Aquaporina 2 Western blot Imunohistoquímica |
| topic |
Nervo facial Paralisia facial Aquaporina 1 Aquaporina 2 Western blot Imunohistoquímica |
| description |
OBJECTIVE: The aim of the study was to describe the expression of aquaporins (AQPs) 1 and 2 in the extra temporal facial nerve(NF) of rats within 7 days after mechanical compression. METHODS: The extra temporal trunk of the right NF of 20 rats was mechanically crushed and after 1, 3 and 7 days, AQP2 expression was analyzed by western blot (WB) (n = 15) and AQP1 expression was analyzed by immunohistochemistry (IHC) (n = 5) in the injured (right) and non injured (left) NF. During this period, the behavioral analysis of the facial paralysis installation in the animals was also performed. RESULTS: WB demonstrated the presence and elevation of AQP2 in injured NF in relation to its control on the first day after neural compression in concomitance with the installation of facial paralysis. Using IHC, the antiAQP1 antibody’s marking occurred mainly in Schwann cells (CS) and endothelial cells. The count of CS marked by the antiAQP1 antibody was higher in the injured nerves compared to the noninjured. CONCLUSION: The concomitance of early elevation of AQP 1 and 2 expressions during the installation of facial`s nerve deficit suggests the possibility that this protein is involved in the physiological events of edema after neural crush. A better understanding of the role of AQPs in NF may lead to the development of new therapies. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-06-28 2020-03-25T12:10:35Z 2020-03-25T12:10:35Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
doctoralThesis |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=6370236 2018-0785.pdf https://repositorio.unifesp.br/handle/11600/52841 |
| dc.identifier.dark.fl_str_mv |
ark:/48912/001300001pgpm |
| url |
https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=6370236 https://repositorio.unifesp.br/handle/11600/52841 |
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2018-0785.pdf ark:/48912/001300001pgpm |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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56 f. application/pdf |
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São Paulo |
| dc.publisher.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
| publisher.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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1848497915974123520 |