Determinação da soroprevalência da infecção pelo vírus da hepatite e em pacientes submetidos à hemodiálise em comparação a transplantados renais e doadores de sangue

• Ano de defesa: 2018
• Autor(a) principal: Sena, Anne de [UNIFESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• dARK ID: ark:/48912/001300002s8cp
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Hepatitis E; Blood donors; Renal transplant; Hemodialysis; Hepatite E; Doadores de sangue; Transplantados renais; Hemodiálise
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=6394640; https://repositorio.unifesp.br/handle/11600/53162

Introduction: Hepatitis E virus (HEV) infection currently accounts for over 50% of acute hepatitis cases in endemic countries. There are cases of hepatic failure in patients with other underlying liver diseases or in women in the third trimester of pregnancy, as well as the chronic form of the disease, especially among solid organ transplant patients. Objective: To establish the seroprevalence of HEV infection in patients submitted to hemodialysis compared to renal transplants patients and healthy controls (blood donors) by detecting antiHEV IgG and IgM antibodies, and in the positive samples the presence of RNAHEV via realtime RTPCR. Methods: 500 blood donors of the Benefit Blood Collection Association, 257 patients undergoing hemodialysis, and 292 kidney transplant recipients from the Hospital of Rim and Hypertension were included in the study. Detection of antiHEV IgG and antiHEV IgM antibodies was performed using the Wantai immunoenzyme assay (Beijing Wantai Biological Pharmacy Enterprise, Beijing, China). Samples with positive antiHEV IgG were submitted to viral RNA extraction for subsequent HEVRNA screening by realtime RTPCR. Results: The seroprevalence of HEV infection in hemodialysis patients was 10.9% when compared to renal transplant recipients (15.8%) and blood donors (9.8%), thus considering age as a risk factor for patients renal transplant patients and patients submitted to hemodialysis presented an increase for the development of hepatitis E in relation to the control group. In none of the seropositive samples was HEV RNA identified. Conclusion: This is the first study of the literature evaluating the seroprevalence of HEV infection in patients submitted to hemodialysis in Brazil and the correlation of the three groups studied shows that age is an important risk factor for the development of the disease.