Avaliação da expressão imuno-histoquímica do fator de crescimento endotelial vascular (VEGF) e da podoplanina em neoplasias intraepiteliais escamosas cervicais de graus 1, 2 e 3

• Ano de defesa: 2016
• Autor(a) principal: Mattos, Patricia Napoli Belfort [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• dARK ID: ark:/48912/001300001pn1r
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: VEGF; Podoplanin; Metastasis; Angiogenesis; Cervical neoplasia; Podoplanina; Metástase; Angiogênese; Imunoexpressão; Lesões intraepiteliais
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=3744151; http://repositorio.unifesp.br/handle/11600/48041

Background: VEGF and podoplanin in invasive lesions of the cervix might beessential to restrict tumor metastatic spread. The research of these markers by immunohistochemistry can assess the immunedetection profile for diagnostic. The aim of the study was to evaluate and correlate the immunoexpression of VEGF and podoplanin in diferents degrees of cervical intraepithelial neoplasia 1, 2 and 3. Patients and Methods: two hundred thirty-four patients with cervical intraepithelial neoplasia 1, 2, 3 and controls were selected and divided em four groups. Clinical data was collected from the patients? charts and the samples from paraffin-embedded to conventional histopathologic method, construction of tissue microarray and immunohistochemical analysis. Results: We evaluated 43 controls samples, 53 samples from CIN 1 group, 60 from CIN 2 and 78 from CIN3, which the ages between 17-62 years old. Comparisons between groups to VEGF immunoexpression we observed difference statistically significant. The occurency of intense VEGF was statistically greater in CIN 2 and CIN 3 groups when compared to CIN 1 and control groups (p<0,001). The intensety of podoplanin immunoexpression between groups showed statistical significance, the negative and focal expression was more present in CIN 3 when compared to CIN 1, CIN 2 and control (p=0.016). In the immunoexpression correlation of the two factors, the podoplanin was equally frequent in different types of VEGF in control, CIN 1 and CIN 2 groups. Patients with CIN 3 presented greater frequency of VEGF moderate to intense among the patients with negative and focal podoplanin expression when compared to diffuse expression. Conclusions: The groups CIN 2 and CIN 3 appears not to present similarity in the occurrence of VEGF intense when compared to CIN 1 and control. Negative and focal podoplanin expression seems to be more present in CIN 3 when compared to other groups. Patients with CIN 3 appears to showed great tendency to moderate and intense VEGF among the patients with negative and focal podoplanin expression when compared to diffuse. .