Identificação de proteínas que interagem com o fator de transcrição GmbZIP105 de soja (Glycine max)

• Ano de defesa: 2014
• Autor(a) principal: Souza, Gilza Barcelos de
• Orientador(a): Fietto, Luciano Gomes
• Banca de defesa: Carvalho, Claudine Márcia , Ribeiro, Cleberson
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Bioquímica Agrícola
• Departamento: Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal
• País: BR
• Palavras-chave em Português: Soja - Resistência; Proteínas; Patógenos; Estresse biótico; Transfatores
• Palavras-chave em Inglês: Soybean - Strength; Proteins; Pathogens; Biotic stress; Transfactors
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
• Link de acesso: http://locus.ufv.br/handle/123456789/2469

Plants are sessile organisms capable of recognizing and attack various pathogens. Foreign organisms recognition or effectors released by them results in Pathogen-Associated Molecular Patterns -triggered immunity or effector-triggered immunity. In response to recognition of the host plant pathogens activate complex signaling pathways of defense, which are often mediated by plant hormones such as auxin, salicylic acid (SA), jasmonic acid (JA), abscisic acid (ABA) and ethylene giberilinas (ET). Other important components of defense pathways are transfatores. The bZIP family members have reported some for being responsive to biotic and abiotic stresses, however, few interactions between proteins that modulate these transfatores are known. Some genes bZIPs groups of C Arabidopsis thaliana and their orthologs are reported in response to biotic stress. Considering similarity of structure with these genes, and are responsive to hormones and biotrophic fungus soybean believe that the bZIP 105 gene has an important role in signaling pathways in response to biotic stress. The objective of this study was to identify proteins that interact with GmbZIP105 two-hybrid assays in yeast using transfator as bait to obtain cDNAs targets in soybean subjected to different stresses library. Three proteins that interact with GmbZIP105 for screening soybean library were identified: uncharacterized protein soybean, asparagine synthetase and auxin repressed protein (ARP). The uncharacterized protein there are no reports about their conserved domains and their biochemical function. Asparagine synthetase protein is described to be responsive to infection by pathogens and treatment with hormones. The third protein was identified a ARP, is a protein repressed by auxin during fruit development, bud dormancy, and activated during the attack nematode. These interactions obtained by in vivo assays indicate that these proteins are likely components of signaling pathways in response to biotic stress, can act positively or negatively modulating the activity GmbZIP105.