Efeito do estresse hídrico na expressão de proteínas em duas variedades de soja contrastantes para o conteúdo de óleo e proteína

• Ano de defesa: 2013
• Autor(a) principal: Soares, Carla Quinhones Godoy
• Orientador(a): Moreira, Maurílio Alves
• Banca de defesa: Ribeiro, Cleberson , Fietto, Luciano Gomes , Ramos, Humberto Josué de Oliveira , Araujo, Wagner Luiz , Teixeira, Arlindo Inês
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Bioquímica Agrícola
• Departamento: Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal
• País: BR
• Palavras-chave em Português: Estresse hidrico; Soja; Proteinas
• Palavras-chave em Inglês: Water stress; Soybeans; Proteins
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL
• Link de acesso: http://locus.ufv.br/handle/123456789/2386

Elucidate the biochemical and physiological processes that occur in response to water stress during plant reproductive development is important for predicting climate impacts on seed quality. In this study, Suprema and UFVTN105AP soybean varieties, which seeds with high oil content and low in protein, and low oil content and high protein content, respectively, were subjected to water deficit in the R5 stage. At this point, physiological parameters were analyzed, leaves and root were collected for two-dimensional gels associated to sequencing by mass spectrometry (MALDI-TOF- TOF), and identification by Mascot ® 4. Real Time Quantitative PCR analyzes were performed, and the relative quantification of gene expression made by 2 - Δct method using the ABI PRISM 7500 SDS thermocycler. Plants were re- watered after this point, and R8 seeds were collected in order to analyze protein and oil content by Near Infrared Spectroscopy (FT- NIR) . Plants subjected to drought have seeds with reduction in oil content in both varieties , and an increase in protein content in the Suprema. Both varieties showed a reduction in the expression of enzymes involved in carbon metabolism , such as phosphoglycerate kinase , glyceraldehyde 3 phosphate dehydrogenase, ferrodixin NADPH reductase, ATP synthase, and quinone oxidoreductase , which are related with the reduction of oil content in seeds. The increased expression of enzymes related to nitrogen metabolism, such as glutamine synthetase, protein 14-3-3, Vspβ, isoflavone reductase and chalcone isomerase, were observed in the Supreme variety, both in leaves and in roots, and were related to increase in the protein content of the seed. Suprema in water stress conditions, showed a high expression of glutamine synthetase in leaves, which was not observed in UFVTN105AP. This fact may be related to the different behavior of the two varieties in relation to the accumulation of protein in the seed. The results show that changes in the seed oil and protein content are related to intensity and duration of the drought stress, and depends on the variety of study.