Anatomia e micromorfologia de Salvinia auriculata Aubl. (Salviniaceae) submetida ao arsênio
Ano de defesa: | 2012 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Viçosa
|
Programa de Pós-Graduação: |
Mestrado em Biologia Celular e Estrutural
|
Departamento: |
Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://locus.ufv.br/handle/123456789/2345 |
Resumo: | Environmental contamination is a serious problem worldwide. Arsenic (As) is a metalloid that has received great attention from researchers due to its high toxicity to live beings. In this context, the use of plants has proven to be an appropriate methodology and cost-effective for the detection of pollutants. However, the use of plant species as bioindicators requires prior knowledge of their characteristics. This study aims to evaluate the responses of Salvinia auriculata Aubl. in the presence of arsenic, determining the content of this element accumulated by species and the possible effects on development, biomass accumulation, morphological alterations and anatomical structure of its leaves. The experimental design was completely randomized with control and four concentrations of As, in the form of sodium arsenate, being 0.5, 1.0, 1.5 and 2.0 mg L-1 with five repetitions, cultivated in Clark nutrient solution during 15 days in the greenhouse. Samples were analyzed by atomic absorption spectrophotometry with hydride generation, to determine the levels of As. Samples of submerged and floating leaves of the first and fourth nodes were collected and processed for microscopic analysis. Aerial parts of the plant was visibly affected, with necrotic areas on the leaf surface, with rounded shape and dark brown tint, whose intensity was directly proportional to the concentration of As. Modified leaf presented gelatinous and browning appearance. Clonal growth rates decreased strongly with increasing concentration of arsenic in the nutrient solution, with a 56.4% reduction in the concentration 0.5 mg L-1, reaching 86.7% at 2.0 mg L- 1. In relation to the accumulation, the absorption of arsenic by plants increased proportionally with the concentration of metal in solution. The microscopic analysis was able to see more dramatic damage in the first node, compared to the fourth node. In the first node may be observed accumulation of cells with content intensely colored by toluidine blue, deformation in the architecture of the epidermal cells, partial damage to trichomes, distortion of the boundary wall of xylem elements. In the fourth node occurred accumulation of contents , changes in the chloroplasts, compression of the gaps, changes in the pattern of development of trichomes with little development and reduction in thickness of the mesophyll. The anatomical changes in leaves and immersed in modified leaves of S. auriculata were evident and can be used for diagnosing injuries and for understanding the mechanisms of phytotoxicity by As. |
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Oliveira, Maria Angélica Guerra dehttp://lattes.cnpq.br/9525029123773599Azevedo, Aristéa Alveshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787822Y7Oliveira, Juraci Alves dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782512D8Araújo, João Marcos dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794558T1Silva, Luzimar Campos dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799707J8Ribeiro, Clebersonhttp://lattes.cnpq.br/50233877640690512015-03-26T13:04:15Z2013-12-122015-03-26T13:04:15Z2012-03-12OLIVEIRA, Maria Angélica Guerra de. Anatomy and morphology of Salvinia auriculata Aubl. (Salviniaceae) submitted to the arsenic. 2012. 47 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2012.http://locus.ufv.br/handle/123456789/2345Environmental contamination is a serious problem worldwide. Arsenic (As) is a metalloid that has received great attention from researchers due to its high toxicity to live beings. In this context, the use of plants has proven to be an appropriate methodology and cost-effective for the detection of pollutants. However, the use of plant species as bioindicators requires prior knowledge of their characteristics. This study aims to evaluate the responses of Salvinia auriculata Aubl. in the presence of arsenic, determining the content of this element accumulated by species and the possible effects on development, biomass accumulation, morphological alterations and anatomical structure of its leaves. The experimental design was completely randomized with control and four concentrations of As, in the form of sodium arsenate, being 0.5, 1.0, 1.5 and 2.0 mg L-1 with five repetitions, cultivated in Clark nutrient solution during 15 days in the greenhouse. Samples were analyzed by atomic absorption spectrophotometry with hydride generation, to determine the levels of As. Samples of submerged and floating leaves of the first and fourth nodes were collected and processed for microscopic analysis. Aerial parts of the plant was visibly affected, with necrotic areas on the leaf surface, with rounded shape and dark brown tint, whose intensity was directly proportional to the concentration of As. Modified leaf presented gelatinous and browning appearance. Clonal growth rates decreased strongly with increasing concentration of arsenic in the nutrient solution, with a 56.4% reduction in the concentration 0.5 mg L-1, reaching 86.7% at 2.0 mg L- 1. In relation to the accumulation, the absorption of arsenic by plants increased proportionally with the concentration of metal in solution. The microscopic analysis was able to see more dramatic damage in the first node, compared to the fourth node. In the first node may be observed accumulation of cells with content intensely colored by toluidine blue, deformation in the architecture of the epidermal cells, partial damage to trichomes, distortion of the boundary wall of xylem elements. In the fourth node occurred accumulation of contents , changes in the chloroplasts, compression of the gaps, changes in the pattern of development of trichomes with little development and reduction in thickness of the mesophyll. The anatomical changes in leaves and immersed in modified leaves of S. auriculata were evident and can be used for diagnosing injuries and for understanding the mechanisms of phytotoxicity by As.A contaminação ambiental constitui um sério problema no mundo inteiro. O arsênio (As) é um metalóide que tem recebido grande atenção de pesquisadores devido a sua elevada toxicidade para os seres vivos. Nesse contexto, o uso de plantas tem se revelado uma metodologia adequada e de baixo custo para a detecção dos poluentes. Entretanto, o uso de espécies vegetais como bioindicadoras requer o conhecimento prévio das suas características. Este trabalho tem a finalidade de avaliar as respostas de Salvinia auriculata Aubl. na presença de arsênio, na forma de arsenato de sódio, determinando o teor deste elemento acumulado pela espécie, bem como os possíveis efeitos sobre o desenvolvimento, o acúmulo de biomassa, as alterações morfológicas e a estrutura anatômica das suas folhas. O delineamento experimental utilizado foi o inteiramente casualizado, com controle e quatro concentrações de As, sendo 0,5; 1,0; 1,5 e 2,0 mg L 1 com cinco repetições, cultivadas em solução nutritiva de Clark, durante 15 dias, em casa de vegetação. Para determinação dos teores de As, amostras foram analisadas por espectrofotometria de absorção atômica com geração de hidreto. Amostras das folhas emersas e submersas do primeiro e do quarto nós foram coletadas e processadas para análise microscópica. A parte aérea foi visivelmente afetada, com áreas necróticas na superfície foliar, com formato arredondado e com tonalidade marrom escura, cuja intensidade foi diretamente proporcional à concentração de As. As folhas modificadas apresentaram aspecto gelatinoso e escurecimento. A absorção de arsênio pelas plantas aumentou proporcionalmente com a concentração do metaloide na solução. Pelas análises microscópicas pôde-se constatar danos mais drásticos no primeiro nó, se comparado ao quarto nó. No primeiro nó, as células tiveram acúmulo de conteúdos intensamente corados pelo azul de toluidina, deformação na arquitetura das células epidérmicas, danos parciais nos tricomas e distorção da parede do contorno dos elementos do xilema. No quarto nó ocorreu acúmulo de conteúdos, alterações nos cloroplastos, compactação das lacunas, alterações no padrão de desenvolvimento dos tricomas com pouco desenvolvimento e redução na espessura do mesofilo. As alterações anatômicas nas folhas emersas e nas folhas modificadas de S. auriculata foram evidentes, podendo ser utilizados no diagnóstico das injúrias e para o entendimento dos mecanismos de fitotoxicidade do As.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Biologia Celular e EstruturalUFVBRAnálises quantitativas e moleculares do Genoma; Biologia das células e dos tecidosSalviniaArsênioMorfologiaSalviniaArsenicMorphologyCNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERALAnatomia e micromorfologia de Salvinia auriculata Aubl. (Salviniaceae) submetida ao arsênioAnatomy and morphology of Salvinia auriculata Aubl. (Salviniaceae) submitted to the arsenicinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1744673https://locus.ufv.br//bitstream/123456789/2345/1/texto%20completo.pdff0c17c926ab7d15f1b82fd4f360a3543MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain69555https://locus.ufv.br//bitstream/123456789/2345/2/texto%20completo.pdf.txt0cb5dba9fa84f16b1c5fa98d961d6439MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3581https://locus.ufv.br//bitstream/123456789/2345/3/texto%20completo.pdf.jpg74638b3a38e1f901d667e6636639e136MD53123456789/23452016-04-08 23:05:51.489oai:locus.ufv.br:123456789/2345Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-09T02:05:51LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Anatomia e micromorfologia de Salvinia auriculata Aubl. (Salviniaceae) submetida ao arsênio |
dc.title.alternative.eng.fl_str_mv |
Anatomy and morphology of Salvinia auriculata Aubl. (Salviniaceae) submitted to the arsenic |
title |
Anatomia e micromorfologia de Salvinia auriculata Aubl. (Salviniaceae) submetida ao arsênio |
spellingShingle |
Anatomia e micromorfologia de Salvinia auriculata Aubl. (Salviniaceae) submetida ao arsênio Oliveira, Maria Angélica Guerra de Salvinia Arsênio Morfologia Salvinia Arsenic Morphology CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Anatomia e micromorfologia de Salvinia auriculata Aubl. (Salviniaceae) submetida ao arsênio |
title_full |
Anatomia e micromorfologia de Salvinia auriculata Aubl. (Salviniaceae) submetida ao arsênio |
title_fullStr |
Anatomia e micromorfologia de Salvinia auriculata Aubl. (Salviniaceae) submetida ao arsênio |
title_full_unstemmed |
Anatomia e micromorfologia de Salvinia auriculata Aubl. (Salviniaceae) submetida ao arsênio |
title_sort |
Anatomia e micromorfologia de Salvinia auriculata Aubl. (Salviniaceae) submetida ao arsênio |
author |
Oliveira, Maria Angélica Guerra de |
author_facet |
Oliveira, Maria Angélica Guerra de |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/9525029123773599 |
dc.contributor.author.fl_str_mv |
Oliveira, Maria Angélica Guerra de |
dc.contributor.advisor-co1.fl_str_mv |
Azevedo, Aristéa Alves |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787822Y7 |
dc.contributor.advisor-co2.fl_str_mv |
Oliveira, Juraci Alves de |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782512D8 |
dc.contributor.advisor1.fl_str_mv |
Araújo, João Marcos de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794558T1 |
dc.contributor.referee1.fl_str_mv |
Silva, Luzimar Campos da |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799707J8 |
dc.contributor.referee2.fl_str_mv |
Ribeiro, Cleberson |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/5023387764069051 |
contributor_str_mv |
Azevedo, Aristéa Alves Oliveira, Juraci Alves de Araújo, João Marcos de Silva, Luzimar Campos da Ribeiro, Cleberson |
dc.subject.por.fl_str_mv |
Salvinia Arsênio Morfologia |
topic |
Salvinia Arsênio Morfologia Salvinia Arsenic Morphology CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Salvinia Arsenic Morphology |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
Environmental contamination is a serious problem worldwide. Arsenic (As) is a metalloid that has received great attention from researchers due to its high toxicity to live beings. In this context, the use of plants has proven to be an appropriate methodology and cost-effective for the detection of pollutants. However, the use of plant species as bioindicators requires prior knowledge of their characteristics. This study aims to evaluate the responses of Salvinia auriculata Aubl. in the presence of arsenic, determining the content of this element accumulated by species and the possible effects on development, biomass accumulation, morphological alterations and anatomical structure of its leaves. The experimental design was completely randomized with control and four concentrations of As, in the form of sodium arsenate, being 0.5, 1.0, 1.5 and 2.0 mg L-1 with five repetitions, cultivated in Clark nutrient solution during 15 days in the greenhouse. Samples were analyzed by atomic absorption spectrophotometry with hydride generation, to determine the levels of As. Samples of submerged and floating leaves of the first and fourth nodes were collected and processed for microscopic analysis. Aerial parts of the plant was visibly affected, with necrotic areas on the leaf surface, with rounded shape and dark brown tint, whose intensity was directly proportional to the concentration of As. Modified leaf presented gelatinous and browning appearance. Clonal growth rates decreased strongly with increasing concentration of arsenic in the nutrient solution, with a 56.4% reduction in the concentration 0.5 mg L-1, reaching 86.7% at 2.0 mg L- 1. In relation to the accumulation, the absorption of arsenic by plants increased proportionally with the concentration of metal in solution. The microscopic analysis was able to see more dramatic damage in the first node, compared to the fourth node. In the first node may be observed accumulation of cells with content intensely colored by toluidine blue, deformation in the architecture of the epidermal cells, partial damage to trichomes, distortion of the boundary wall of xylem elements. In the fourth node occurred accumulation of contents , changes in the chloroplasts, compression of the gaps, changes in the pattern of development of trichomes with little development and reduction in thickness of the mesophyll. The anatomical changes in leaves and immersed in modified leaves of S. auriculata were evident and can be used for diagnosing injuries and for understanding the mechanisms of phytotoxicity by As. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-03-12 |
dc.date.available.fl_str_mv |
2013-12-12 2015-03-26T13:04:15Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:04:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Maria Angélica Guerra de. Anatomy and morphology of Salvinia auriculata Aubl. (Salviniaceae) submitted to the arsenic. 2012. 47 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2012. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/2345 |
identifier_str_mv |
OLIVEIRA, Maria Angélica Guerra de. Anatomy and morphology of Salvinia auriculata Aubl. (Salviniaceae) submitted to the arsenic. 2012. 47 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2012. |
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http://locus.ufv.br/handle/123456789/2345 |
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Universidade Federal de Viçosa |
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Mestrado em Biologia Celular e Estrutural |
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UFV |
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BR |
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Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos |
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Universidade Federal de Viçosa |
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