Efeitos do exercício físico sobre propriedades estruturais e mecânicas do fêmur de ratos com Diabetes mellitus experimental

• Ano de defesa: 2011
• Autor(a) principal: Silva, Karina Ana da
• Orientador(a): Natali, Antônio José
• Banca de defesa: Neves, Clóvis Andrade
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Educação Física
• Departamento: Aspectos sócio-culturais do movimento humano; Aspectos biodinâmicos do movimento humano
• País: BR
• Palavras-chave em Português: Atividade física; Tecido ósseo; Natação; Fratura óssea
• Palavras-chave em Inglês: Physical activity; Bone; Swimming; Bone breaking
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::EDUCACAO FISICA
• Link de acesso: http://locus.ufv.br/handle/123456789/3468

The aim of this study was to investigate the effects of a swimming training program on structural and mechanical properties of the femur from rats with experimental diabetes mellitus (DM). Thirty days old male Wistar rats (Rattus norvegicus) with mean body mass of 87.42 g were randomly divided into six experimental groups: baseline control (BC, n=15); baseline diabetic (BD, n=15); control sedentary (CS, n=10); control exercised (CE, n=10); diabetic sedentary (DS, n=10); and diabetic exercised (DE, n=10). Animals from BD, DE and DS groups received an intraperitoneal injection (60 mg/kg body mass) of streptozotocin (STZ) diluted in 1 mL of sodium citrate buffer (0.1 M, pH 4.5). Animals from BC, SC and EC groups received the same dose of sodium citrate buffer (0.1 M, pH 4.5) without STZ. Seven days after STZ injection those animals with fasting glucose above 300 mg\dL were considered diabetic. After 45 days of hyperglycemia (diabetes), animals from ED and EC groups were subjected to a swimming training program for 8 weeks. After euthanasia, the left femur was removed for assessment of bone mineral density (BMD), bone mineral content (BMC), bone breaking force, stiffness and toughness. The right femur was removed for determination of bone morphometry. The results show that experimental DM reduced the bone breaking force, stiffness and toughness, the BMC and BMD, the trabecular bone volume and cortical thickness in the rats’ femurs by the 45th day of DM. These DM effects remained until the end of the experiment (101 days). The swimming training program increased BMC, BMD, stiffness and the trabecular bone volume in the control animal femures. However, the swimming training program did not affect these bone parameters in diabetic animals. It was concluded that the swimming training program did not affect the structural and mechanical properties of the femur from rats with experimental DM, however positive effects of this exercise program on these bone properties were observed in non diabetic control rats.