Estudo dos mecanismos envolvidos na adaptabilidade diferencial de dois begomovírus em tomateiro e Nicotiana benthamiana
Ano de defesa: | 2008 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Viçosa
|
Programa de Pós-Graduação: |
Doutorado em Fitopatologia
|
Departamento: |
Etiologia; Epidemiologia; Controle
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://locus.ufv.br/handle/123456789/1015 |
Resumo: | Begomoviruses belong to the family Geminiviridae, which includes viruses with a circular, single-stranded DNA genome encapsidated in twinned icosahedral particles. Begomoviruses are transmitted by the whitefly Bemisia tabaci, and cause serious diseases in several economically important crops, mostly in tropical and subtropical regions. In Brazil, a viral complex comprising at least eight species, including Tomato rugose mosaic virus (ToRMV) and Tomato yellow spot virus (ToYSV), is responsible for great losses in tomato production. ToYSV symptoms in tomato and Nicotiana benthamiana are more severe and appear earlier compared to ToRMV, suggesting differential adaptation of each virus to their hosts. We performed a series of experiments in order to analyze the role of a number of factors in this differential adaptation. The kinetics of viral infection was analyzed in plants which had the inoculated leaf removed at various periods of time after inoculation. Viral DNA accumulation was estimated in plants at 14 and 28 days post-inoculation (dpi). Viral replication was analyzed in protoplasts at 48 and 96 hours post- electroporation (hpe). Viral tissue tropism was analyzed by in situ hybridization at 14 dpi. Results indicate that ToYSV is more efficient in carrying out the early (pre-systemic) events of infection, reaching a higher concentration and establishing a systemic infection sooner and more efficiently than ToRMV in both tomato and N. benthamiana. In mixed infections, ToRMV negatively interferes on ToYSV during these initial stages of infection, but once a systemic infection is established this negative interference ceases. Both viruses are phloem-restricted in tomato. In N. benthamiana, ToYSV invades the mesophyll, while ToRMV is phloem-restricted. During mixed infection in this host, ToYSV releases ToRMV from the phloem. We conclude that ToYSV is better adapted than ToRMV to both hosts. In tomato, this is expressed by its reaching a higher concentration in a shorter period of time, which may lead to a higher accumulation of viral virulence factors. In N. benthamiana, it is further expressed by its ability to invade mesophyll cells. |
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Alves Júnior, Miguelhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4766837Y7Lamêgo, Márcia Rogéria de Almeidahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782197P4Carvalho, Murilo Geraldo dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787250U5Zerbini Júnior, Francisco Murilohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5Zerbini, Poliane Alfenashttp://lattes.cnpq.br/8632328159533071Andrade, Eduardo Chumbinho dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767872E8Otoni, Wagner Camposhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786133Y62015-03-26T12:41:47Z2008-05-262015-03-26T12:41:47Z2008-02-29ALVES JÚNIOR, Miguel. Study of the mechanisms involved in the diferential adaptability of two begomoviruses to tomato and Nicotiana benthamiana. 2008. 80 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2008.http://locus.ufv.br/handle/123456789/1015Begomoviruses belong to the family Geminiviridae, which includes viruses with a circular, single-stranded DNA genome encapsidated in twinned icosahedral particles. Begomoviruses are transmitted by the whitefly Bemisia tabaci, and cause serious diseases in several economically important crops, mostly in tropical and subtropical regions. In Brazil, a viral complex comprising at least eight species, including Tomato rugose mosaic virus (ToRMV) and Tomato yellow spot virus (ToYSV), is responsible for great losses in tomato production. ToYSV symptoms in tomato and Nicotiana benthamiana are more severe and appear earlier compared to ToRMV, suggesting differential adaptation of each virus to their hosts. We performed a series of experiments in order to analyze the role of a number of factors in this differential adaptation. The kinetics of viral infection was analyzed in plants which had the inoculated leaf removed at various periods of time after inoculation. Viral DNA accumulation was estimated in plants at 14 and 28 days post-inoculation (dpi). Viral replication was analyzed in protoplasts at 48 and 96 hours post- electroporation (hpe). Viral tissue tropism was analyzed by in situ hybridization at 14 dpi. Results indicate that ToYSV is more efficient in carrying out the early (pre-systemic) events of infection, reaching a higher concentration and establishing a systemic infection sooner and more efficiently than ToRMV in both tomato and N. benthamiana. In mixed infections, ToRMV negatively interferes on ToYSV during these initial stages of infection, but once a systemic infection is established this negative interference ceases. Both viruses are phloem-restricted in tomato. In N. benthamiana, ToYSV invades the mesophyll, while ToRMV is phloem-restricted. During mixed infection in this host, ToYSV releases ToRMV from the phloem. We conclude that ToYSV is better adapted than ToRMV to both hosts. In tomato, this is expressed by its reaching a higher concentration in a shorter period of time, which may lead to a higher accumulation of viral virulence factors. In N. benthamiana, it is further expressed by its ability to invade mesophyll cells.Os begomovírus pertencem à família Geminiviridae, que inclui vírus com genoma de DNA circular de fita simples, encapsidado em partículas icosaédricas geminadas. Os begomovírus são transmitidos pela mosca-branca Bemisia tabaci, e causam doenças de grande importância econômica em diversas culturas, principalmente em regiões tropicais e subtropicais. No Brasil, um complexo viral composto por pelo menos oito espécies, incluindo o Tomato rugose mosaic virus (ToRMV) e o Tomato yellow spot virus (ToYSV), é responsável por grandes perdas na cultura do tomateiro. Os sintomas induzidos pelo ToYSV em tomateiro e Nicotiana benthamiana são mais severos e surgem mais cedo em comparação aos induzidos pelo ToRMV, o que sugere uma adaptação diferencial de cada vírus aos seus hospedeiros. Neste trabalho, foram conduzidos experimentos com o objetivo de analisar o papel de diversos fatores nessa adaptação diferencial. A cinética da infecção viral foi analisada em plantas nas quais a folha inoculada foi removida após diversos períodos de tempo. O acúmulo de DNA viral foi estimado em plantas infectadas, aos 14 e 28 dias pósinoculação (dpi). A replicação viral foi analisada em protoplastos, a 48 e 96 horas póseletroporação (hpe). O tropismo de tecido viral foi analisado por meio de hibridização in situ aos 14 dpi. Os resultados indicam que o ToYSV é mais eficiente durante os eventos iniciais (pré-infecção sistêmica) do ciclo de infecção, atingindo uma concentração maior e estabelecendo a infecção sistêmica mais rapidamente em relação ao ToRMV, tanto em tomateiro quanto em N. benthamiana. Em infecções mistas, o ToRMV interfere negativamente com o ToYSV durante os estágios iniciais da infecção, mas essa interferência cessa após o estabelecimento da infecção sistêmica. Ambos os vírus são restritos ao floema em tomateiro. Em N. benthamiana, o ToYSV invade o mesofilo, enquanto o ToRMV é restrito ao floema. Na infecção mista nesse hospedeiro, o ToRMV passa a infectar células do mesofilo. Em conjunto, esses resultados confirmam a hipótese de que o ToYSV é melhor adaptado a ambos os hospedeiros em relação ao ToRMV. Em tomateiro, essa melhor adaptação é expressa na forma de maior eficiência nos processos iniciais de infecção, permitindo que o ToYSV atinja maior concentração em um período de tempo mais curto, o que talvez leve a um maior acúmulo de fatores de virulência virais. Em N. benthamiana, a melhor adaptação é expressa também pela capacidade de invadir os tecidos do mesofilo.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaDoutorado em FitopatologiaUFVBREtiologia; Epidemiologia; ControleGeminivirusInteração patógeno-hospedeiroGeminivirusHost-pathogen interactionCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIAEstudo dos mecanismos envolvidos na adaptabilidade diferencial de dois begomovírus em tomateiro e Nicotiana benthamianaStudy of the mechanisms involved in the diferential adaptability of two begomoviruses to tomato and Nicotiana benthamianainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1302800https://locus.ufv.br//bitstream/123456789/1015/1/texto%20completo.pdf068caeb9eaa8bb91cb97a256edbc25faMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain144342https://locus.ufv.br//bitstream/123456789/1015/2/texto%20completo.pdf.txt2c84e796602efb5608d457d24a1d8bfdMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3528https://locus.ufv.br//bitstream/123456789/1015/3/texto%20completo.pdf.jpg27d65203cbf6b3e92338487aa61645d3MD53123456789/10152016-04-06 23:16:57.707oai:locus.ufv.br:123456789/1015Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-07T02:16:57LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Estudo dos mecanismos envolvidos na adaptabilidade diferencial de dois begomovírus em tomateiro e Nicotiana benthamiana |
dc.title.alternative.eng.fl_str_mv |
Study of the mechanisms involved in the diferential adaptability of two begomoviruses to tomato and Nicotiana benthamiana |
title |
Estudo dos mecanismos envolvidos na adaptabilidade diferencial de dois begomovírus em tomateiro e Nicotiana benthamiana |
spellingShingle |
Estudo dos mecanismos envolvidos na adaptabilidade diferencial de dois begomovírus em tomateiro e Nicotiana benthamiana Alves Júnior, Miguel Geminivirus Interação patógeno-hospedeiro Geminivirus Host-pathogen interaction CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA |
title_short |
Estudo dos mecanismos envolvidos na adaptabilidade diferencial de dois begomovírus em tomateiro e Nicotiana benthamiana |
title_full |
Estudo dos mecanismos envolvidos na adaptabilidade diferencial de dois begomovírus em tomateiro e Nicotiana benthamiana |
title_fullStr |
Estudo dos mecanismos envolvidos na adaptabilidade diferencial de dois begomovírus em tomateiro e Nicotiana benthamiana |
title_full_unstemmed |
Estudo dos mecanismos envolvidos na adaptabilidade diferencial de dois begomovírus em tomateiro e Nicotiana benthamiana |
title_sort |
Estudo dos mecanismos envolvidos na adaptabilidade diferencial de dois begomovírus em tomateiro e Nicotiana benthamiana |
author |
Alves Júnior, Miguel |
author_facet |
Alves Júnior, Miguel |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4766837Y7 |
dc.contributor.author.fl_str_mv |
Alves Júnior, Miguel |
dc.contributor.advisor-co1.fl_str_mv |
Lamêgo, Márcia Rogéria de Almeida |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782197P4 |
dc.contributor.advisor-co2.fl_str_mv |
Carvalho, Murilo Geraldo de |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787250U5 |
dc.contributor.advisor1.fl_str_mv |
Zerbini Júnior, Francisco Murilo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5 |
dc.contributor.referee1.fl_str_mv |
Zerbini, Poliane Alfenas |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/8632328159533071 |
dc.contributor.referee2.fl_str_mv |
Andrade, Eduardo Chumbinho de |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767872E8 |
dc.contributor.referee3.fl_str_mv |
Otoni, Wagner Campos |
dc.contributor.referee3Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786133Y6 |
contributor_str_mv |
Lamêgo, Márcia Rogéria de Almeida Carvalho, Murilo Geraldo de Zerbini Júnior, Francisco Murilo Zerbini, Poliane Alfenas Andrade, Eduardo Chumbinho de Otoni, Wagner Campos |
dc.subject.por.fl_str_mv |
Geminivirus Interação patógeno-hospedeiro |
topic |
Geminivirus Interação patógeno-hospedeiro Geminivirus Host-pathogen interaction CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA |
dc.subject.eng.fl_str_mv |
Geminivirus Host-pathogen interaction |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA |
description |
Begomoviruses belong to the family Geminiviridae, which includes viruses with a circular, single-stranded DNA genome encapsidated in twinned icosahedral particles. Begomoviruses are transmitted by the whitefly Bemisia tabaci, and cause serious diseases in several economically important crops, mostly in tropical and subtropical regions. In Brazil, a viral complex comprising at least eight species, including Tomato rugose mosaic virus (ToRMV) and Tomato yellow spot virus (ToYSV), is responsible for great losses in tomato production. ToYSV symptoms in tomato and Nicotiana benthamiana are more severe and appear earlier compared to ToRMV, suggesting differential adaptation of each virus to their hosts. We performed a series of experiments in order to analyze the role of a number of factors in this differential adaptation. The kinetics of viral infection was analyzed in plants which had the inoculated leaf removed at various periods of time after inoculation. Viral DNA accumulation was estimated in plants at 14 and 28 days post-inoculation (dpi). Viral replication was analyzed in protoplasts at 48 and 96 hours post- electroporation (hpe). Viral tissue tropism was analyzed by in situ hybridization at 14 dpi. Results indicate that ToYSV is more efficient in carrying out the early (pre-systemic) events of infection, reaching a higher concentration and establishing a systemic infection sooner and more efficiently than ToRMV in both tomato and N. benthamiana. In mixed infections, ToRMV negatively interferes on ToYSV during these initial stages of infection, but once a systemic infection is established this negative interference ceases. Both viruses are phloem-restricted in tomato. In N. benthamiana, ToYSV invades the mesophyll, while ToRMV is phloem-restricted. During mixed infection in this host, ToYSV releases ToRMV from the phloem. We conclude that ToYSV is better adapted than ToRMV to both hosts. In tomato, this is expressed by its reaching a higher concentration in a shorter period of time, which may lead to a higher accumulation of viral virulence factors. In N. benthamiana, it is further expressed by its ability to invade mesophyll cells. |
publishDate |
2008 |
dc.date.available.fl_str_mv |
2008-05-26 2015-03-26T12:41:47Z |
dc.date.issued.fl_str_mv |
2008-02-29 |
dc.date.accessioned.fl_str_mv |
2015-03-26T12:41:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ALVES JÚNIOR, Miguel. Study of the mechanisms involved in the diferential adaptability of two begomoviruses to tomato and Nicotiana benthamiana. 2008. 80 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2008. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/1015 |
identifier_str_mv |
ALVES JÚNIOR, Miguel. Study of the mechanisms involved in the diferential adaptability of two begomoviruses to tomato and Nicotiana benthamiana. 2008. 80 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2008. |
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http://locus.ufv.br/handle/123456789/1015 |
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por |
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Universidade Federal de Viçosa |
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Doutorado em Fitopatologia |
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UFV |
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BR |
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Etiologia; Epidemiologia; Controle |
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Universidade Federal de Viçosa |
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