Avaliação histológica e imunofluorescente da lesão compressiva na medula espinhal de ratos wistar tratados com células-tronco mesenquimais

• Ano de defesa: 2013
• Autor(a) principal: Araújo, Marta Rocha
• Orientador(a): Neves, Clóvis Andrade
• Banca de defesa: Monteiro, Betânia Souza , Carlo, Ricardo Junqueira Del
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Biologia Celular e Estrutural
• Departamento: Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos
• País: BR
• Palavras-chave em Português: Lesão medular; Terapia celular; Célula-tronco mesenquimal
• Palavras-chave em Inglês: Spinal cord injury; Cell therapy; Mesenchymal stem cell
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: http://locus.ufv.br/handle/123456789/2358

The traumatic spinal cord injury often results in severe neurological deficits, and mostly irreversible. The cell therapy represents a new strategy for the treatment of these lesions, particularly with the use of stem cells (SC), with satisfactory results in several experimental models. This study compared the spinal cord injury in animals treated and not treated with mesenchymal stem cells (MSC) from bone marrow. MSCs were obtained from bone marrow of rats, cultured, characterized, labeled with Qtracker 655® and transplanted in the sixth passage in animals with spinal cord injury.Spinal cord injury (SCI) was performed with 2-Fr Fogarty catheter after T9-T10 laminectomy. The animals were randomly divided into six experimental groups of 5 animals each and submitted to different treatments: control group (CG), with only spinal cord injury and treated groups (GT) that suffered the SCI and received transplants of cells 3 hours after injury. The animals were euthanized 24 hours, 7 and 21 days postinjury. For light microscopy, was measured the percentage of lesion area in the fragment of macroscopic lesion. We also investigated, by fluorescence microscopy, the effect of MSC on inflammation, astrocytic reactivity and activation of endogenous stem cells. There was no statistical difference between GC and MSC as the size of the lesion area. Although MSC were not found at the site of the lesion, in the evaluation immunofluorescent was seen in the animals treated with MSC was significant attenuation of the inflammatory response with a reduction in the number of macrophages labeled with anti-CD68 antibody (ED1), decreased immunoreactivity of astrocytes (GFAP+) and greater activation of endogenous stem cells (nestin+). Our results indicate that cell transplantation has a beneficial effect on recovery from traumatic spinal cord injury. As MSC transplanted nanolabeling were not observed at the lesion site, we suggest that the therapeutic effect of these cells can occur by some telecrine mechanism.