Acumulação e acessibilidade de minerais em cogumelos de Pleurotus ostreatus enriquecidos com ferro, zinco e lítio
Ano de defesa: | 2010 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Viçosa
|
Programa de Pós-Graduação: |
Mestrado em Microbiologia Agrícola
|
Departamento: |
Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://locus.ufv.br/handle/123456789/5324 |
Resumo: | Pleurotus ostreatus mushrooms are food with excellent nutritional value that may be enriched contributing to the mobility, bioavailability and transfer of various chemicals elements from the environment for other organisms. Mineral supplements and fortified foods have been used in order to ensure the necessary amount of essential microelements that may be lacking in the diet. Iron (Fe) and zinc (Zn) are important minerals for human health and although lithium (Li) has no known nutritional function, an appropriate supply of this metal would provide behavioral benefits. Thus the enrichment of mushrooms of P. ostreatus with these elements may be useful for obtaining a concentrated and bioavailable source of these microelements. Thus, the objectives were to enrich mushrooms of P. ostreatus grown on substrates based on coffee husk supplemented with Fe, Zn or Li, to evaluate the accumulation capacity of these elements in mushrooms and evaluate the accessibility of these elements in relation to the commercial mineral supplements. The mushrooms were produced on substrate based on coffee husk supplemented with different concentrations of ferrous sulfate (FeSO4) (0, 0.4, 0.6, 0.8, 1.0 or 2.0 mg kg-1), zinc carbonate (ZnCO3) (0, 0.53, 1.07, 2.13, 4.27 or 8.53 mg kg-1) and lithium chloride (LiCl) (0, 62.25; 125, 250, 500 or 1000 mg kg-1). The biological efficiency (BE), the proteins contents and concentration of Fe, Zn, Li, and also of Mn, K, P, Ca, Cu, Pb, Cd, S, Cr, Mg, Ni and Al, in the mushrooms in three consecutive harvests were determined. To evaluate the accessibility of Fe, Zn and Li, two procedures were performed: the sequential extraction and in vitro digestibility. Samples of the mushrooms enriched with FeSO4, ZnCO3 or LiCl were evaluated in comparison with to three commercial mineral supplements containing FeSO4, zinc oxide (ZnO) or lithium carbonate (Li2CO3). The biological efficiency (BE) was higher in the first harvest and reduced considerably in the third harvest, not being influenced by different concentrations of FeSO4, ZnCO3 or LiCl (P > 0.05). The accumulation of Fe was affected by both dose of FeSO4 added to the substrate and the order of haverts (P < 0.05). The mushroom of P. ostreatus accumulated Zn and Li, and concentrations were influenced by the increasing concentration of ZnCO3 and LiCl in the substrate (P < 0.05). The Al, Cu, Mn, Cr, Pb, Ni and Cd were not detected in mushrooms enriched with FeSO4, ZnCO3 or LiCl. The concentrations of S, K and P were affected by different doses of FeSO4, ZnCO3 or LiCl added to the substrate (P < 0.05), the Ca and Mg were affected by different concentrations of FeSO4 and ZnCO3 (P < 0.05). The proteins content was only affected by the dose of FeSO4 added to the husk of coffee (P < 0.05). The elements Fe, Zn and Li in both, enriched and unenriched mushrooms showed greater accessibility than those same elements present in their respective supplements. The mushroom of P. ostreatus can be an alternative source of Fe, Zn and Li with high accessibility. |
id |
UFV_dbbdc6322c266420825109a56a34b916 |
---|---|
oai_identifier_str |
oai:locus.ufv.br:123456789/5324 |
network_acronym_str |
UFV |
network_name_str |
LOCUS Repositório Institucional da UFV |
repository_id_str |
|
spelling |
Assunção, Laélia Soares dehttp://lattes.cnpq.br/4982550637380646Bazzolli, Denise Mara Soareshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4761710D6Vanetti, Maria Cristina Dantashttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783874H3Kasuya, Maria Catarina Megumihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721444T5Santos, Miriam Teresinha doshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786872Z0Fontes, Edimar Aparecida Filomenohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792339T8Costa, Neuza Maria Brunorohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781709D62015-03-26T13:51:50Z2011-09-022015-03-26T13:51:50Z2010-02-25ASSUNÇÃO, Laélia Soares de. Accumulation and accessibility of minerals in Pleurotus ostreatus mushrooms enriched with iron, zinc and lithium.. 2010. 73 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2010.http://locus.ufv.br/handle/123456789/5324Pleurotus ostreatus mushrooms are food with excellent nutritional value that may be enriched contributing to the mobility, bioavailability and transfer of various chemicals elements from the environment for other organisms. Mineral supplements and fortified foods have been used in order to ensure the necessary amount of essential microelements that may be lacking in the diet. Iron (Fe) and zinc (Zn) are important minerals for human health and although lithium (Li) has no known nutritional function, an appropriate supply of this metal would provide behavioral benefits. Thus the enrichment of mushrooms of P. ostreatus with these elements may be useful for obtaining a concentrated and bioavailable source of these microelements. Thus, the objectives were to enrich mushrooms of P. ostreatus grown on substrates based on coffee husk supplemented with Fe, Zn or Li, to evaluate the accumulation capacity of these elements in mushrooms and evaluate the accessibility of these elements in relation to the commercial mineral supplements. The mushrooms were produced on substrate based on coffee husk supplemented with different concentrations of ferrous sulfate (FeSO4) (0, 0.4, 0.6, 0.8, 1.0 or 2.0 mg kg-1), zinc carbonate (ZnCO3) (0, 0.53, 1.07, 2.13, 4.27 or 8.53 mg kg-1) and lithium chloride (LiCl) (0, 62.25; 125, 250, 500 or 1000 mg kg-1). The biological efficiency (BE), the proteins contents and concentration of Fe, Zn, Li, and also of Mn, K, P, Ca, Cu, Pb, Cd, S, Cr, Mg, Ni and Al, in the mushrooms in three consecutive harvests were determined. To evaluate the accessibility of Fe, Zn and Li, two procedures were performed: the sequential extraction and in vitro digestibility. Samples of the mushrooms enriched with FeSO4, ZnCO3 or LiCl were evaluated in comparison with to three commercial mineral supplements containing FeSO4, zinc oxide (ZnO) or lithium carbonate (Li2CO3). The biological efficiency (BE) was higher in the first harvest and reduced considerably in the third harvest, not being influenced by different concentrations of FeSO4, ZnCO3 or LiCl (P > 0.05). The accumulation of Fe was affected by both dose of FeSO4 added to the substrate and the order of haverts (P < 0.05). The mushroom of P. ostreatus accumulated Zn and Li, and concentrations were influenced by the increasing concentration of ZnCO3 and LiCl in the substrate (P < 0.05). The Al, Cu, Mn, Cr, Pb, Ni and Cd were not detected in mushrooms enriched with FeSO4, ZnCO3 or LiCl. The concentrations of S, K and P were affected by different doses of FeSO4, ZnCO3 or LiCl added to the substrate (P < 0.05), the Ca and Mg were affected by different concentrations of FeSO4 and ZnCO3 (P < 0.05). The proteins content was only affected by the dose of FeSO4 added to the husk of coffee (P < 0.05). The elements Fe, Zn and Li in both, enriched and unenriched mushrooms showed greater accessibility than those same elements present in their respective supplements. The mushroom of P. ostreatus can be an alternative source of Fe, Zn and Li with high accessibility.Cogumelos de Pleurotus ostreatus constituem um alimento com excelente valor nutritivo que podem ser enriquecidos, contribuindo para a mobilidade, biodisponibilidade e transferência de diversos elementos químicos do ambiente para outros organismos. Suplementos minerais e alimentos enriquecidos têm sido utilizados com o intuito de garantir a quantidade necessária de minerais essenciais que possam estar em deficiência na dieta. O ferro (Fe) e o zinco (Zn) são minerais importantes para a saúde humana e, embora o lítio (Li) não possua função nutricional reconhecida, um aporte adequado desse metal pode fornecer benefícios comportamentais. Dessa maneira, o enriquecimento de cogumelos de P. ostreatus com Fe, Zn ou Li pode ser útil na obtenção de uma fonte concentrada e biodisponível desses microelementos. Assim, os objetivos deste trabalho foram enriquecer cogumelos de P. ostreatus cultivados em substratos à base de casca de café suplementados com Fe, Zn ou Li, avaliar a capacidade de acumulação desses elementos nos cogumelos e verificar a acessibilidade desses elementos em relação a suplementos minerais comerciais. Os cogumelos foram produzidos em substrato à base de casca de café adicionado de diferentes concentrações de sulfato ferroso (FeSO4) (0; 0,4; 0,6; 0,8; 1,0 ou 2,0 mg kg-1); carbonato de zinco (ZnCO3) (0; 0,53; 1,07; 2,13; 4,27 ou 8,53 mg kg-1); ou cloreto de lítio (LiCl) (0; 62,25; 125; 250; 500 ou 1000 mg kg-1). Determinou-se a eficiência biológica (EB), o teor de proteínas, as concentrações de Fe, Zn, Li e também de Mn, K, P, Ca, Cu, Pb, Cd, S, Cr, Mg, Ni e Al, em três colheitas consecutivas. Para verificar a acessibilidade do Fe, Zn e Li, duas metodologias foram realizadas, a extração sequencial e a digestibilidade in vitro. Amostras dos cogumelos enriquecidos com FeSO4, ZnCO3 ou LiCl foram avaliadas em comparação a três suplementos minerais comerciais contendo FeSO4, óxido de zinco (ZnO) ou carbonato de lítio (Li2CO3). A eficiência biológica (EB) foi maior na primeira colheita e reduziu consideravelmente na terceira colheita, não sendo influenciada pelas diferentes concentrações de FeSO4, ZnCO3 ou LiCl (P > 0,05). A acumulação de Fe foi afetada tanto pela dose de FeSO4 adicionada ao substrato quanto pela ordem das colheitas (P < 0,05).O cogumelo de P. ostreatus acumulou Zn e Li, e a concentração foi influenciada pelo aumento da concentração de ZnCO3 e LiCl adicionada ao substrato (P < 0,05). Não foram observadas a presença de Al, Cu, Mn, Cr, Pb, Ni e Cd nos cogumelos enriquecidos com FeSO4, ZnCO3 ou LiCl. Os teores de S, K e P foram afetados pelas diferentes doses de FeSO4, ZnCO3 ou LiCl adicionadas ao substrato (P < 0,05), sendo os teores de Ca e Mg afetados pelas diferentes concentrações de FeSO4 e ZnCO3 (P < 0,05). O teor de proteínas foi somente afetado pela dose de FeSO4 adicionada ao substrato casca de café (P < 0,05). Os elementos Fe, Zn e Li presentes tanto nos cogumelos enriquecido quanto nos cogumelos não enriquecidos, apresentaram acessibilidade maior que esses mesmos microelementos presentes em seus respectivos suplementos. O cogumelo de P. ostreatus pode ser uma fonte alternativa de Fe, Zn e Li de alta acessibilidade.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaMestrado em Microbiologia AgrícolaUFVBRAssociações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interessePleurotus ostreatusAcumulaçãoEnriquecimentoFerroZincoLítioPleurotus ostreatusAccumulationEnrichmentIronZincLithiumCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO::MICROBIOLOGIA E BIOQUIMICA DO SOLOAcumulação e acessibilidade de minerais em cogumelos de Pleurotus ostreatus enriquecidos com ferro, zinco e lítioAccumulation and accessibility of minerals in Pleurotus ostreatus mushrooms enriched with iron, zinc and lithium.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf514409https://locus.ufv.br//bitstream/123456789/5324/1/texto%20completo.pdf79049d9859fbc9a8836af68b6536b119MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain117073https://locus.ufv.br//bitstream/123456789/5324/2/texto%20completo.pdf.txt925ee5aa6b3eccd820393bb070f82fa6MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3664https://locus.ufv.br//bitstream/123456789/5324/3/texto%20completo.pdf.jpgf7ba440073aab0ce9328d9a375f8a430MD53123456789/53242016-04-10 23:17:38.363oai:locus.ufv.br:123456789/5324Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:17:38LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Acumulação e acessibilidade de minerais em cogumelos de Pleurotus ostreatus enriquecidos com ferro, zinco e lítio |
dc.title.alternative.eng.fl_str_mv |
Accumulation and accessibility of minerals in Pleurotus ostreatus mushrooms enriched with iron, zinc and lithium. |
title |
Acumulação e acessibilidade de minerais em cogumelos de Pleurotus ostreatus enriquecidos com ferro, zinco e lítio |
spellingShingle |
Acumulação e acessibilidade de minerais em cogumelos de Pleurotus ostreatus enriquecidos com ferro, zinco e lítio Assunção, Laélia Soares de Pleurotus ostreatus Acumulação Enriquecimento Ferro Zinco Lítio Pleurotus ostreatus Accumulation Enrichment Iron Zinc Lithium CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO::MICROBIOLOGIA E BIOQUIMICA DO SOLO |
title_short |
Acumulação e acessibilidade de minerais em cogumelos de Pleurotus ostreatus enriquecidos com ferro, zinco e lítio |
title_full |
Acumulação e acessibilidade de minerais em cogumelos de Pleurotus ostreatus enriquecidos com ferro, zinco e lítio |
title_fullStr |
Acumulação e acessibilidade de minerais em cogumelos de Pleurotus ostreatus enriquecidos com ferro, zinco e lítio |
title_full_unstemmed |
Acumulação e acessibilidade de minerais em cogumelos de Pleurotus ostreatus enriquecidos com ferro, zinco e lítio |
title_sort |
Acumulação e acessibilidade de minerais em cogumelos de Pleurotus ostreatus enriquecidos com ferro, zinco e lítio |
author |
Assunção, Laélia Soares de |
author_facet |
Assunção, Laélia Soares de |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/4982550637380646 |
dc.contributor.author.fl_str_mv |
Assunção, Laélia Soares de |
dc.contributor.advisor-co1.fl_str_mv |
Bazzolli, Denise Mara Soares |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4761710D6 |
dc.contributor.advisor-co2.fl_str_mv |
Vanetti, Maria Cristina Dantas |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783874H3 |
dc.contributor.advisor1.fl_str_mv |
Kasuya, Maria Catarina Megumi |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721444T5 |
dc.contributor.referee1.fl_str_mv |
Santos, Miriam Teresinha dos |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786872Z0 |
dc.contributor.referee2.fl_str_mv |
Fontes, Edimar Aparecida Filomeno |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792339T8 |
dc.contributor.referee3.fl_str_mv |
Costa, Neuza Maria Brunoro |
dc.contributor.referee3Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781709D6 |
contributor_str_mv |
Bazzolli, Denise Mara Soares Vanetti, Maria Cristina Dantas Kasuya, Maria Catarina Megumi Santos, Miriam Teresinha dos Fontes, Edimar Aparecida Filomeno Costa, Neuza Maria Brunoro |
dc.subject.por.fl_str_mv |
Pleurotus ostreatus Acumulação Enriquecimento Ferro Zinco Lítio |
topic |
Pleurotus ostreatus Acumulação Enriquecimento Ferro Zinco Lítio Pleurotus ostreatus Accumulation Enrichment Iron Zinc Lithium CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO::MICROBIOLOGIA E BIOQUIMICA DO SOLO |
dc.subject.eng.fl_str_mv |
Pleurotus ostreatus Accumulation Enrichment Iron Zinc Lithium |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO::MICROBIOLOGIA E BIOQUIMICA DO SOLO |
description |
Pleurotus ostreatus mushrooms are food with excellent nutritional value that may be enriched contributing to the mobility, bioavailability and transfer of various chemicals elements from the environment for other organisms. Mineral supplements and fortified foods have been used in order to ensure the necessary amount of essential microelements that may be lacking in the diet. Iron (Fe) and zinc (Zn) are important minerals for human health and although lithium (Li) has no known nutritional function, an appropriate supply of this metal would provide behavioral benefits. Thus the enrichment of mushrooms of P. ostreatus with these elements may be useful for obtaining a concentrated and bioavailable source of these microelements. Thus, the objectives were to enrich mushrooms of P. ostreatus grown on substrates based on coffee husk supplemented with Fe, Zn or Li, to evaluate the accumulation capacity of these elements in mushrooms and evaluate the accessibility of these elements in relation to the commercial mineral supplements. The mushrooms were produced on substrate based on coffee husk supplemented with different concentrations of ferrous sulfate (FeSO4) (0, 0.4, 0.6, 0.8, 1.0 or 2.0 mg kg-1), zinc carbonate (ZnCO3) (0, 0.53, 1.07, 2.13, 4.27 or 8.53 mg kg-1) and lithium chloride (LiCl) (0, 62.25; 125, 250, 500 or 1000 mg kg-1). The biological efficiency (BE), the proteins contents and concentration of Fe, Zn, Li, and also of Mn, K, P, Ca, Cu, Pb, Cd, S, Cr, Mg, Ni and Al, in the mushrooms in three consecutive harvests were determined. To evaluate the accessibility of Fe, Zn and Li, two procedures were performed: the sequential extraction and in vitro digestibility. Samples of the mushrooms enriched with FeSO4, ZnCO3 or LiCl were evaluated in comparison with to three commercial mineral supplements containing FeSO4, zinc oxide (ZnO) or lithium carbonate (Li2CO3). The biological efficiency (BE) was higher in the first harvest and reduced considerably in the third harvest, not being influenced by different concentrations of FeSO4, ZnCO3 or LiCl (P > 0.05). The accumulation of Fe was affected by both dose of FeSO4 added to the substrate and the order of haverts (P < 0.05). The mushroom of P. ostreatus accumulated Zn and Li, and concentrations were influenced by the increasing concentration of ZnCO3 and LiCl in the substrate (P < 0.05). The Al, Cu, Mn, Cr, Pb, Ni and Cd were not detected in mushrooms enriched with FeSO4, ZnCO3 or LiCl. The concentrations of S, K and P were affected by different doses of FeSO4, ZnCO3 or LiCl added to the substrate (P < 0.05), the Ca and Mg were affected by different concentrations of FeSO4 and ZnCO3 (P < 0.05). The proteins content was only affected by the dose of FeSO4 added to the husk of coffee (P < 0.05). The elements Fe, Zn and Li in both, enriched and unenriched mushrooms showed greater accessibility than those same elements present in their respective supplements. The mushroom of P. ostreatus can be an alternative source of Fe, Zn and Li with high accessibility. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-02-25 |
dc.date.available.fl_str_mv |
2011-09-02 2015-03-26T13:51:50Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:51:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ASSUNÇÃO, Laélia Soares de. Accumulation and accessibility of minerals in Pleurotus ostreatus mushrooms enriched with iron, zinc and lithium.. 2010. 73 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2010. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/5324 |
identifier_str_mv |
ASSUNÇÃO, Laélia Soares de. Accumulation and accessibility of minerals in Pleurotus ostreatus mushrooms enriched with iron, zinc and lithium.. 2010. 73 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2010. |
url |
http://locus.ufv.br/handle/123456789/5324 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.publisher.program.fl_str_mv |
Mestrado em Microbiologia Agrícola |
dc.publisher.initials.fl_str_mv |
UFV |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse |
publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
bitstream.url.fl_str_mv |
https://locus.ufv.br//bitstream/123456789/5324/1/texto%20completo.pdf https://locus.ufv.br//bitstream/123456789/5324/2/texto%20completo.pdf.txt https://locus.ufv.br//bitstream/123456789/5324/3/texto%20completo.pdf.jpg |
bitstream.checksum.fl_str_mv |
79049d9859fbc9a8836af68b6536b119 925ee5aa6b3eccd820393bb070f82fa6 f7ba440073aab0ce9328d9a375f8a430 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1801213838010351616 |