Detection and evaluation of genetic diversity of Porcine circovirus 3 in Brazilian swine farms, expression and purification of the capsid protein

Detalhes bibliográficos
Ano de defesa: 2021
Autor(a) principal: Assao, Viviane Sisdelli
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: eng
Instituição de defesa: Universidade Federal de Viçosa
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Suíno - Doenças - Diagnóstico
Proteínas recombinantes
Porcine circovirus
Medicina Veterinária Preventiva
Link de acesso: https://locus.ufv.br//handle/123456789/28161
Resumo: Porcine circovirus 3 (PCV3) is a recently discovered virus. PCV3 has been associated with several signs, but its pathogenicity is still being debated. PCV3 has been detected and circulating in the swine population worldwide. Due to the fact that there are a few studies of PCV3 in Brazilian swine herds, this work aimed to investigat PCV3 in Brazil. We evaluated two molecular diagnostic tests (real- time PCR and Nested PCR) for PCV3 detection, and the real-time PCR assay had better results than the Nested PCR assay used in this study. We investigated the genetic diversity of PCV3 in swine farms from Minas Gerais, the fourth largest pork producing state in Brazil. Our results revealed the presence of at least two PCV3 main lineages circulating in Brazil. Furthermore, for the first time, we reported the detection of two different PCV3 strains in the same pig. We also surveyed PCV3 in swine farms affected by several health problems in Paraná, the second-largest pork producing state in Brazil. Our results showed that 36.88% of the swine were PCV3 positive. PCV3 DNA was detected in swine with different health problems, including animals with vesicular lesions that had not been previously documented. Finally, we expressed the truncated PCV3 capsid protein using a prokaryotic expression system and partially purified it using a two-steps purification with the goal for future development of vaccines and diagnostic tests. Keywords: Porcine circovirus 3. Genetic diversity. Real-time PCR. Nested PCR. Capsid protein.
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spelling Detection and evaluation of genetic diversity of Porcine circovirus 3 in Brazilian swine farms, expression and purification of the capsid proteinDetecção e avaliação da diversidade genética de Porcine circovírus 3 em fazendas de suínos brasileiras, expressão e purificação da proteína do capsídeoSuíno - Doenças - DiagnósticoProteínas recombinantesPorcine circovirusMedicina Veterinária PreventivaPorcine circovirus 3 (PCV3) is a recently discovered virus. PCV3 has been associated with several signs, but its pathogenicity is still being debated. PCV3 has been detected and circulating in the swine population worldwide. Due to the fact that there are a few studies of PCV3 in Brazilian swine herds, this work aimed to investigat PCV3 in Brazil. We evaluated two molecular diagnostic tests (real- time PCR and Nested PCR) for PCV3 detection, and the real-time PCR assay had better results than the Nested PCR assay used in this study. We investigated the genetic diversity of PCV3 in swine farms from Minas Gerais, the fourth largest pork producing state in Brazil. Our results revealed the presence of at least two PCV3 main lineages circulating in Brazil. Furthermore, for the first time, we reported the detection of two different PCV3 strains in the same pig. We also surveyed PCV3 in swine farms affected by several health problems in Paraná, the second-largest pork producing state in Brazil. Our results showed that 36.88% of the swine were PCV3 positive. PCV3 DNA was detected in swine with different health problems, including animals with vesicular lesions that had not been previously documented. Finally, we expressed the truncated PCV3 capsid protein using a prokaryotic expression system and partially purified it using a two-steps purification with the goal for future development of vaccines and diagnostic tests. Keywords: Porcine circovirus 3. Genetic diversity. Real-time PCR. Nested PCR. Capsid protein.Porcine circovirus 3 (PCV3) é um vírus que foi descoberto recentemente. PCV3 tem sido associado a diversos sintomas, mas a sua patogenicidade ainda está sendo discutida. PCV3 tem sido detectado circulando na população suína em todo o mundo. Devido ao fato de que existem poucos estudos sobre PCV3 no rebanho suíno brasileiro, esse trabalho teve como objetivo estudar a presença de PCV3 no Brasil. Neste trabalho nós investigamos a diversidade genética de PCV3 em fazendas de suínos localizadas em Minas Gerais. Os resultados mostraram que pelo menos duas linhagens de cepas de PCV3 estão circulando no Brasil. Pela primeira vez, um estudo relata a detecção de duas cepas diferentes de PCV3 no mesmo suíno. Nós avaliamos duas técnicas de diagnóstico molecular (real-time PCR e Nested PCR) para a detecção de PCV3, e a técnica de real-time PCR obteve resultados superiores em comparação com a técnica de Nested PCR usada neste estudo. Nós investigamos a presença de PCV3 em fazendas de suínos que relatavam problemas sanitários no Paraná, o segundo maior estado produtor de carne suína. Nossos resultados mostraram que 36,88% dos suínos eram positivos para PCV3. O DNA de PCV3 foi detectado em suínos com diversos problemas de saúde, incluindo suínos com lesões vesiculares o que não foi descrito em trabalhos anteriores. Além disso, nós expressamos a proteína truncada do capsídeo viral de PCV3 usando um sistema de expressão procarioto e purificamos parcialmente a proteína usando um protocolo de purificação em duas etapas para ser utilizada no futuro para o desenvolvimento de vacinas e testes de diagnóstico. Palavras-chave: Porcine circovirus 3. Diversidade genética. Real-time PCR. Nested PCR. Proteína do capsídeo.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorUniversidade Federal de ViçosaSilva Júnior, Abelardohttp://lattes.cnpq.br/9301935595881071Yamatogi, Ricardo SeitiMoreira, Maria Aparecida ScatamburloAssao, Viviane Sisdelli2021-08-31T19:50:33Z2021-08-31T19:50:33Z2021-02-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfASSAO, Viviane Sisdelli. Detection and evaluation of genetic diversity of Porcine circovirus 3 in Brazilian swine farms, expression and purification of the capsid protein. 2021. 113 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Viçosa, Viçosa. 2021.https://locus.ufv.br//handle/123456789/28161enginfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:43:20Zoai:locus.ufv.br:123456789/28161Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:43:20LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Detection and evaluation of genetic diversity of Porcine circovirus 3 in Brazilian swine farms, expression and purification of the capsid protein
Detecção e avaliação da diversidade genética de Porcine circovírus 3 em fazendas de suínos brasileiras, expressão e purificação da proteína do capsídeo
title Detection and evaluation of genetic diversity of Porcine circovirus 3 in Brazilian swine farms, expression and purification of the capsid protein
spellingShingle Detection and evaluation of genetic diversity of Porcine circovirus 3 in Brazilian swine farms, expression and purification of the capsid protein
Assao, Viviane Sisdelli
Suíno - Doenças - Diagnóstico
Proteínas recombinantes
Porcine circovirus
Medicina Veterinária Preventiva
title_short Detection and evaluation of genetic diversity of Porcine circovirus 3 in Brazilian swine farms, expression and purification of the capsid protein
title_full Detection and evaluation of genetic diversity of Porcine circovirus 3 in Brazilian swine farms, expression and purification of the capsid protein
title_fullStr Detection and evaluation of genetic diversity of Porcine circovirus 3 in Brazilian swine farms, expression and purification of the capsid protein
title_full_unstemmed Detection and evaluation of genetic diversity of Porcine circovirus 3 in Brazilian swine farms, expression and purification of the capsid protein
title_sort Detection and evaluation of genetic diversity of Porcine circovirus 3 in Brazilian swine farms, expression and purification of the capsid protein
author Assao, Viviane Sisdelli
author_facet Assao, Viviane Sisdelli
author_role author
dc.contributor.none.fl_str_mv Silva Júnior, Abelardo
http://lattes.cnpq.br/9301935595881071
Yamatogi, Ricardo Seiti
Moreira, Maria Aparecida Scatamburlo
dc.contributor.author.fl_str_mv Assao, Viviane Sisdelli
dc.subject.por.fl_str_mv Suíno - Doenças - Diagnóstico
Proteínas recombinantes
Porcine circovirus
Medicina Veterinária Preventiva
topic Suíno - Doenças - Diagnóstico
Proteínas recombinantes
Porcine circovirus
Medicina Veterinária Preventiva
description Porcine circovirus 3 (PCV3) is a recently discovered virus. PCV3 has been associated with several signs, but its pathogenicity is still being debated. PCV3 has been detected and circulating in the swine population worldwide. Due to the fact that there are a few studies of PCV3 in Brazilian swine herds, this work aimed to investigat PCV3 in Brazil. We evaluated two molecular diagnostic tests (real- time PCR and Nested PCR) for PCV3 detection, and the real-time PCR assay had better results than the Nested PCR assay used in this study. We investigated the genetic diversity of PCV3 in swine farms from Minas Gerais, the fourth largest pork producing state in Brazil. Our results revealed the presence of at least two PCV3 main lineages circulating in Brazil. Furthermore, for the first time, we reported the detection of two different PCV3 strains in the same pig. We also surveyed PCV3 in swine farms affected by several health problems in Paraná, the second-largest pork producing state in Brazil. Our results showed that 36.88% of the swine were PCV3 positive. PCV3 DNA was detected in swine with different health problems, including animals with vesicular lesions that had not been previously documented. Finally, we expressed the truncated PCV3 capsid protein using a prokaryotic expression system and partially purified it using a two-steps purification with the goal for future development of vaccines and diagnostic tests. Keywords: Porcine circovirus 3. Genetic diversity. Real-time PCR. Nested PCR. Capsid protein.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-31T19:50:33Z
2021-08-31T19:50:33Z
2021-02-25
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv ASSAO, Viviane Sisdelli. Detection and evaluation of genetic diversity of Porcine circovirus 3 in Brazilian swine farms, expression and purification of the capsid protein. 2021. 113 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Viçosa, Viçosa. 2021.
https://locus.ufv.br//handle/123456789/28161
identifier_str_mv ASSAO, Viviane Sisdelli. Detection and evaluation of genetic diversity of Porcine circovirus 3 in Brazilian swine farms, expression and purification of the capsid protein. 2021. 113 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Viçosa, Viçosa. 2021.
url https://locus.ufv.br//handle/123456789/28161
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa
publisher.none.fl_str_mv Universidade Federal de Viçosa
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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