Morfometria do órgão vomeronasal e do testículo, comportamento sexual de coelhos e perfis de testosterona e androstenediona após cauterização do ducto incisivo

• Ano de defesa: 2012
• Autor(a) principal: Grondona, Káterin Elena Bohorquez
• Orientador(a): Benjamin, Laércio dos Anjos
• Banca de defesa: Costa, Eduardo Paulino da , Espeschit, Claudio José Borela , Avelar, Gleide Fernandes de
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Medicina Veterinária
• Departamento: Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de
• País: BR
• Palavras-chave em Português: Orgão vomeronasal; Testículo; Morfometria
• Palavras-chave em Inglês: Vomeronasal organ; Testis; Morphometrics
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
• Link de acesso: http://locus.ufv.br/handle/123456789/1456

In the domestic rabbit (Oryctolagus cuniculus), the vomeronasal organ ends in the rostral part of the palate through the incisive duct. This duct allows pheromones to enrich and stimulate this organ. The obstruction (cauterization) of the incisive duct causes morphological alteration in the vomeronasal organ and in the testis. In order to develop the present study, incisive ducts of 16 New Zealand male rabbits (60 days of age) were cauterized under anesthesia. Sixteen rabbits from the same breed and age were used as a control group. Hence, 8 animals of each group (control and cauterized) were sacrificed at two different time points: 120 and 180 days of age (d). Before sacrifice, the animals were anesthetized and perfused through the aortic artery with bicarbonate Ringer s solution, followed by a 2.5% glutaraldehyde solution and testes, vomeronasal organ, skeletal muscle and bone were collected. These tissues were submitted to routine procedures for histological studies, transmission electron microscopy, also stereological and morphometrical techniques. Based on the serum testosterone dosages, it was found that the 120d group presented no difference in androgen levels; however, the 180d group showed a variation on levels of serum testosterone. Hence, in order to better understand the results, the 180d group was subdivided in low (180d-LT) and high (180d-HT) testosterone concentration. Apparently, the cauterization procedure did not affect the volume density of the components as well as the epithelium height of vomeronasal organ. However there are some evidence of neuronal loss, based on spaces in the apical portion the sensorial epithelium and elongated, dense, and irregular borders of nuclei of the supporting cells. The tubular diameter was lower in 120d and 180d-HT when compared with the control group. The In comparison to the control, Leydig cell number was higher in the 180d-LT and lower in the 120d group. Also, the volume densities of the testicular parameters were lower in the 120d when compared with control. Regarding to Sertoli cell, it was observed a decrease in the percentage occupied in the testis parenchyma in 120d and 180d-LT groups. . Considering the muscle skeletal system, there are no differences in the bone density of the femoral epiphyses and also in the mass and composition of the femoral muscle. These results demonstrate that much about the VNO remains to be elucidated in the different species of mammals, in which the VNO can act in a different form, as in the pig, which pheromones are essential for sexual stimulation of both males and females.