Estudo da citotoxicidade e biointerface de nanopartículas de dióxido de titânio utilizadas nas indústrias de cosméticos
Ano de defesa: | 2017 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade do Grande Rio
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Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ensino das Ciências
|
Departamento: |
Unigranrio::Ensino das Ciências
|
País: |
Brasil
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://localhost:8080/tede/handle/tede/369 |
Resumo: | The development of the nanotechnology area, in particular in whatregards the production of nanomaterials, is growing exponentially. Human exposure to nanoparticles is high and originated from a variety of sources, such as chemical processes, medical devices (implants, prostheses, controlled drug delivery systems), food, pharmaceutical and cosmetic industries. However, the study of the cytotoxicity of these nanomaterials is contradictory. Therefore, it is extremely important to develop research that analyzes the impact of nanoparticles on human health, as well as generating regulations that up to now is insufficient. Currently, the sunscreens have in their composition nanoparticles such as those of titanium dioxide (TiO2).The use of TiO2 in sunscreen formulation is due to its ability to reflect and spread UVB (290-320 nm) and UVA (320-400 nm) rays ensuring good protection against solar radiation. Therefore, this work aimed to evaluate the potential cytotoxicity of TiO2 nanoparticles in the rutile crystalline structure in human skin cells. Due to the agglomeration of nanoparticles, a nanoparticle dispersion protocol was optimized, first in water and then in biological medium using an indirect sonicator. The dispersion was then characterized by dynamic light scattering (DLS) and electronic transmission microscopy (MET). A study of ions and proteins adsorption was carried out by energy dispersivex-ray spectroscopy (EDS) and mass spectrometry, respectively. We performed an evaluation of the nanoparticles cytotoxic profile in primary cultures of fibroblasts and keratinocytes by the neutral red assay and by flow cytometry. MET allowed the study of the nanoparticles internalization inside cells. The results demonstrate the formation of a bio-complex around nanoparticles surfaces due to the selective adsorption of ions and proteins from the culture medium, such as calcium and phosphorus ions and proteins as albumin, fibronectin and thrombospondin. We observed that human keratinocytes and fibroblasts internalized the nanoparticles, which located in microvesicles that migrate preferentially to the perinuclear region. However, after nanoparticle internalization, there was a significant increase in cell death due to apoptosis and a significant reduction in cell viability in the fibroblasts, as well as increased apoptosis death and necrosis in keratinocytes. We suggest that the bio-complex formation can mask the nanoparticles and modulate their penetration into human skin cells. |
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Ribeiro, Ana Rosa Lopes PereiraBenchimol, MarleneTakamori, Esther RiekoSant’Anna Filho, Celso Barbosa deGranjeiro, José MauroSanches, Priscila Laviola2020-01-07T18:10:33Z2017-05-24SANCHES, Priscila Laviola. Estudo da citotoxicidade e biointerface de nanopartículas de dióxido de titânio utilizadas nas indústrias de cosméticos. 2017. 98 f. Dissertação (Mestrado em Biomedicina Translacional) - Universidade do Grande Rio, Duque de Caxias, 2017.http://localhost:8080/tede/handle/tede/369The development of the nanotechnology area, in particular in whatregards the production of nanomaterials, is growing exponentially. Human exposure to nanoparticles is high and originated from a variety of sources, such as chemical processes, medical devices (implants, prostheses, controlled drug delivery systems), food, pharmaceutical and cosmetic industries. However, the study of the cytotoxicity of these nanomaterials is contradictory. Therefore, it is extremely important to develop research that analyzes the impact of nanoparticles on human health, as well as generating regulations that up to now is insufficient. Currently, the sunscreens have in their composition nanoparticles such as those of titanium dioxide (TiO2).The use of TiO2 in sunscreen formulation is due to its ability to reflect and spread UVB (290-320 nm) and UVA (320-400 nm) rays ensuring good protection against solar radiation. Therefore, this work aimed to evaluate the potential cytotoxicity of TiO2 nanoparticles in the rutile crystalline structure in human skin cells. Due to the agglomeration of nanoparticles, a nanoparticle dispersion protocol was optimized, first in water and then in biological medium using an indirect sonicator. The dispersion was then characterized by dynamic light scattering (DLS) and electronic transmission microscopy (MET). A study of ions and proteins adsorption was carried out by energy dispersivex-ray spectroscopy (EDS) and mass spectrometry, respectively. We performed an evaluation of the nanoparticles cytotoxic profile in primary cultures of fibroblasts and keratinocytes by the neutral red assay and by flow cytometry. MET allowed the study of the nanoparticles internalization inside cells. The results demonstrate the formation of a bio-complex around nanoparticles surfaces due to the selective adsorption of ions and proteins from the culture medium, such as calcium and phosphorus ions and proteins as albumin, fibronectin and thrombospondin. We observed that human keratinocytes and fibroblasts internalized the nanoparticles, which located in microvesicles that migrate preferentially to the perinuclear region. However, after nanoparticle internalization, there was a significant increase in cell death due to apoptosis and a significant reduction in cell viability in the fibroblasts, as well as increased apoptosis death and necrosis in keratinocytes. We suggest that the bio-complex formation can mask the nanoparticles and modulate their penetration into human skin cells.O desenvolvimento da área de nanotecnologia, em particular no que diz respeito à produção de nanomateriais, encontra-se em crescimento exponencial. A exposição humana a nanopartículas é elevada e originada de diversas fontes, tais como processos químicos, dispositivos médicos (implantes, próteses, sistemas de liberação controlada de fármacos), indústria alimentar, farmacêutica e de cosméticos. Entretanto, os resultados dos estudos da citotoxicidade destes nanomateriais é muitas das vezes contraditório. Desta forma, é de extrema importância o desenvolvimento de pesquisas que analisem o impacto de nanopartículas na saúde humana, assim como gerar regulamentação que até ao momento é insuficiente. Atualmente, os protetores solares apresentam na sua composição nanopartículas como as de dióxido de titânio (TiO2). A utilização do TiO2 em fórmulas de filtros solares deve-se à sua capacidade de refletir e espalhar raios UVB (290–320 nm) e UVA (320–400 nm) garantindo uma boa proteção contra a radiação solar. Portanto, este trabalho teve como objetivo avaliar a potencial citotoxicidade de nanopartículas de TiO2 na estrutura cristalina de rutilo em células de pele humana. Devido à aglomeração das nanopartículas, foi otimizado um protocolo de dispersão das nanopartículas, primeiro em água e depois em meio biológico, utilizando um sonicador no modo indireto. A dispersão foi, em seguida, caracterizada por espalhamento de luz dinâmico (DLS) e microscopia eletrônica de transmissão (MET). Um estudo de adsorção de íons e proteínas foi realizado pela técnica de espetroscopia por energia dispersiva (EDS) e por espectrometria de massas, respectivamente. A avaliação do perfil citotóxico das nanopartículas em culturas primárias de fibroblastos e queratinócitos foi realizada pelo ensaio de vermelho neutro e por citometria de fluxo. Os estudos da internalização das nanopartículas no interior de células foram realizados por MET. Os resultados demonstram que houve formação de um bio-complexo nas superfícies das nanopartículas devido à adsorção seletiva de íons e proteínas do meio de cultivo, como por exemplo, os íons de cálcio e fósforo e proteínas como a albumina, fibronectina e trombospondina. Foi observado também que as nanopartículas foram internalizadas por queratinócitos e fibroblastos primários da pele humana e migram preferencialmente para a região perinuclear. Após a internalização das nanopartículas, houve um aumento significativo da morte celular por apoptose nos fibroblastos e redução significativa da viabilidade celular, assim como aumento de morte por apoptose e necrose nos queratinócitos, para as concentrações mais altas de nanopartículas. Supõe-se que a formação do bio-complexo pode mascarar as nanopartículas e modular a penetração das mesmas nas células da pele humana.Submitted by Janser dos Santos Nascimento (janser.nascimento@unigranrio.com.br) on 2020-01-07T18:10:33Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Priscila Laviola Sanches.pdf: 2756259 bytes, checksum: 161c38ecb5ded6dd78b024bc8a61037f (MD5)Made available in DSpace on 2020-01-07T18:10:33Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Priscila Laviola Sanches.pdf: 2756259 bytes, checksum: 161c38ecb5ded6dd78b024bc8a61037f (MD5) Previous issue date: 2017-05-24CNPqapplication/pdfporUniversidade do Grande RioPrograma de Pós-Graduação em Ensino das CiênciasUNIGRANRIOBrasilUnigranrio::Ensino das Ciênciashttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessBiomedicinaNanopartículasDióxido de titânioCIÊNCIAS DA SAÚDEEstudo da citotoxicidade e biointerface de nanopartículas de dióxido de titânio utilizadas nas indústrias de cosméticosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisreponame:Biblioteca Digital de Teses e Dissertações da UNIGRANRIOinstname:Universidade do Grande Rio (UNIGRANRIO)instacron:UNIGRANRIOLICENSElicense.txtlicense.txttext/plain; charset=utf-81982http://localhost:8080/tede/bitstream/tede/369/1/license.txt4a50535e8405f611398e6e2d408dbd1bMD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://localhost:8080/tede/bitstream/tede/369/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://localhost:8080/tede/bitstream/tede/369/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://localhost:8080/tede/bitstream/tede/369/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54ORIGINALPriscila Laviola Sanches.pdfPriscila Laviola Sanches.pdfapplication/pdf2756259http://localhost:8080/tede/bitstream/tede/369/5/Priscila+Laviola+Sanches.pdf161c38ecb5ded6dd78b024bc8a61037fMD55tede/3692020-01-07 16:10:33.078oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unigranrio.edu.br/PUBhttp://tede.unigranrio.edu.br/oai/requestrepositorio@instituicao.br||repositorio@instituicao.bropendoar:2020-01-07T18:10:33Biblioteca Digital de Teses e Dissertações da UNIGRANRIO - Universidade do Grande Rio (UNIGRANRIO)false |
dc.title.por.fl_str_mv |
Estudo da citotoxicidade e biointerface de nanopartículas de dióxido de titânio utilizadas nas indústrias de cosméticos |
title |
Estudo da citotoxicidade e biointerface de nanopartículas de dióxido de titânio utilizadas nas indústrias de cosméticos |
spellingShingle |
Estudo da citotoxicidade e biointerface de nanopartículas de dióxido de titânio utilizadas nas indústrias de cosméticos Sanches, Priscila Laviola Biomedicina Nanopartículas Dióxido de titânio CIÊNCIAS DA SAÚDE |
title_short |
Estudo da citotoxicidade e biointerface de nanopartículas de dióxido de titânio utilizadas nas indústrias de cosméticos |
title_full |
Estudo da citotoxicidade e biointerface de nanopartículas de dióxido de titânio utilizadas nas indústrias de cosméticos |
title_fullStr |
Estudo da citotoxicidade e biointerface de nanopartículas de dióxido de titânio utilizadas nas indústrias de cosméticos |
title_full_unstemmed |
Estudo da citotoxicidade e biointerface de nanopartículas de dióxido de titânio utilizadas nas indústrias de cosméticos |
title_sort |
Estudo da citotoxicidade e biointerface de nanopartículas de dióxido de titânio utilizadas nas indústrias de cosméticos |
author |
Sanches, Priscila Laviola |
author_facet |
Sanches, Priscila Laviola |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ribeiro, Ana Rosa Lopes Pereira |
dc.contributor.advisor-co1.fl_str_mv |
Benchimol, Marlene |
dc.contributor.referee1.fl_str_mv |
Takamori, Esther Rieko |
dc.contributor.referee2.fl_str_mv |
Sant’Anna Filho, Celso Barbosa de |
dc.contributor.referee3.fl_str_mv |
Granjeiro, José Mauro |
dc.contributor.author.fl_str_mv |
Sanches, Priscila Laviola |
contributor_str_mv |
Ribeiro, Ana Rosa Lopes Pereira Benchimol, Marlene Takamori, Esther Rieko Sant’Anna Filho, Celso Barbosa de Granjeiro, José Mauro |
dc.subject.por.fl_str_mv |
Biomedicina Nanopartículas Dióxido de titânio |
topic |
Biomedicina Nanopartículas Dióxido de titânio CIÊNCIAS DA SAÚDE |
dc.subject.cnpq.fl_str_mv |
CIÊNCIAS DA SAÚDE |
description |
The development of the nanotechnology area, in particular in whatregards the production of nanomaterials, is growing exponentially. Human exposure to nanoparticles is high and originated from a variety of sources, such as chemical processes, medical devices (implants, prostheses, controlled drug delivery systems), food, pharmaceutical and cosmetic industries. However, the study of the cytotoxicity of these nanomaterials is contradictory. Therefore, it is extremely important to develop research that analyzes the impact of nanoparticles on human health, as well as generating regulations that up to now is insufficient. Currently, the sunscreens have in their composition nanoparticles such as those of titanium dioxide (TiO2).The use of TiO2 in sunscreen formulation is due to its ability to reflect and spread UVB (290-320 nm) and UVA (320-400 nm) rays ensuring good protection against solar radiation. Therefore, this work aimed to evaluate the potential cytotoxicity of TiO2 nanoparticles in the rutile crystalline structure in human skin cells. Due to the agglomeration of nanoparticles, a nanoparticle dispersion protocol was optimized, first in water and then in biological medium using an indirect sonicator. The dispersion was then characterized by dynamic light scattering (DLS) and electronic transmission microscopy (MET). A study of ions and proteins adsorption was carried out by energy dispersivex-ray spectroscopy (EDS) and mass spectrometry, respectively. We performed an evaluation of the nanoparticles cytotoxic profile in primary cultures of fibroblasts and keratinocytes by the neutral red assay and by flow cytometry. MET allowed the study of the nanoparticles internalization inside cells. The results demonstrate the formation of a bio-complex around nanoparticles surfaces due to the selective adsorption of ions and proteins from the culture medium, such as calcium and phosphorus ions and proteins as albumin, fibronectin and thrombospondin. We observed that human keratinocytes and fibroblasts internalized the nanoparticles, which located in microvesicles that migrate preferentially to the perinuclear region. However, after nanoparticle internalization, there was a significant increase in cell death due to apoptosis and a significant reduction in cell viability in the fibroblasts, as well as increased apoptosis death and necrosis in keratinocytes. We suggest that the bio-complex formation can mask the nanoparticles and modulate their penetration into human skin cells. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-05-24 |
dc.date.accessioned.fl_str_mv |
2020-01-07T18:10:33Z |
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info:eu-repo/semantics/publishedVersion |
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dc.identifier.citation.fl_str_mv |
SANCHES, Priscila Laviola. Estudo da citotoxicidade e biointerface de nanopartículas de dióxido de titânio utilizadas nas indústrias de cosméticos. 2017. 98 f. Dissertação (Mestrado em Biomedicina Translacional) - Universidade do Grande Rio, Duque de Caxias, 2017. |
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http://localhost:8080/tede/handle/tede/369 |
identifier_str_mv |
SANCHES, Priscila Laviola. Estudo da citotoxicidade e biointerface de nanopartículas de dióxido de titânio utilizadas nas indústrias de cosméticos. 2017. 98 f. Dissertação (Mestrado em Biomedicina Translacional) - Universidade do Grande Rio, Duque de Caxias, 2017. |
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