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Ação antifúngica do óleo essencial de Rosa alba L.

Detalhes bibliográficos
Ano de defesa: 2011
Autor(a) principal: Reis, Oswaldo Henrique Barolli lattes
Orientador(a): Miranda, Adélia Pereira lattes
Banca de defesa: Fiorini, João Evangelista lattes, Delu Filho, Nelson lattes
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Jose do Rosario Vellano
Programa de Pós-Graduação: Programa de Mestrado em Ciência Animal
Departamento: Zootecnia e Recursos Pesqueiros
País: BR
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://tede2.unifenas.br:8080/jspui/handle/jspui/122
Resumo: In swine culture, the study of mycology contributes to food security in the understanding of various diseases. A knowledge of fungal agents on the skin of healthy animals and surface pathologies is important for effective control of the potential risks of infection, especially when associated with confined species, where the imbalance in the microbiota of the environment can be crucial in the interactions between environment and animals. The objective was to investigate the in vitro antimicrobial activity of essential oil of Rosa alba L. petals on microbial growth of yeasts Candida albicans (ATCC 10231) and filamentous fungi that causes opportunistic infectious processes in the skin of pigs, such as Microsporum nanum (ATCC 11832), Trichophyton mentagrophytes (ATCC 9533), Trichophyton rubrum (ATCC 10218) and Trichophyton tonsurans (ATCC 10870). The oils were extracted by the process of hydrodistillation in Clevenger apparatus, using fresh white rose petals. Microbiological tests were performed by the agar diffusion method (pour-plate) with Sabouraud-dextrose. Wells 5 mm in diameter, produced in the agar, were filled with 15 μL of essential oil and the plates containing filamentous fungi were incubated in an inverted position at 28 °C for 10 days and the plates containing yeast were incubated at 30 °C for 48 hours. After this time, the inhibition circles were measured. The data were subjected to analysis of variance and means were compared by Tukey`s test at 5% probability. It was found that the activity of essential oil under study is significant in relation to the growth of microbial species tested.
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spelling Miranda, Adélia PereiraCPF:03567810626http://lattes.cnpq.br/9885391923578742Fiorini, João EvangelistaCPF:05657512634http://lattes.cnpq.br/6320803631314170Delu Filho, NelsonCPF:06633253826http://lattes.cnpq.br/4783763747882936CPF:85971642634http://lattes.cnpq.br/3343193387948447Reis, Oswaldo Henrique Barolli2016-05-02T13:55:30Z2011-11-162011-02-23REIS, Oswaldo Henrique Barolli. Antifungal action of essential oil of Rosa alba L.. 2011. 71 f. Dissertação (Mestrado em Zootecnia e Recursos Pesqueiros) - Universidade Jose do Rosario Vellano, Alfenas, 2011.http://tede2.unifenas.br:8080/jspui/handle/jspui/122In swine culture, the study of mycology contributes to food security in the understanding of various diseases. A knowledge of fungal agents on the skin of healthy animals and surface pathologies is important for effective control of the potential risks of infection, especially when associated with confined species, where the imbalance in the microbiota of the environment can be crucial in the interactions between environment and animals. The objective was to investigate the in vitro antimicrobial activity of essential oil of Rosa alba L. petals on microbial growth of yeasts Candida albicans (ATCC 10231) and filamentous fungi that causes opportunistic infectious processes in the skin of pigs, such as Microsporum nanum (ATCC 11832), Trichophyton mentagrophytes (ATCC 9533), Trichophyton rubrum (ATCC 10218) and Trichophyton tonsurans (ATCC 10870). The oils were extracted by the process of hydrodistillation in Clevenger apparatus, using fresh white rose petals. Microbiological tests were performed by the agar diffusion method (pour-plate) with Sabouraud-dextrose. Wells 5 mm in diameter, produced in the agar, were filled with 15 μL of essential oil and the plates containing filamentous fungi were incubated in an inverted position at 28 °C for 10 days and the plates containing yeast were incubated at 30 °C for 48 hours. After this time, the inhibition circles were measured. The data were subjected to analysis of variance and means were compared by Tukey`s test at 5% probability. It was found that the activity of essential oil under study is significant in relation to the growth of microbial species tested.Na suinocultura, o estudo da micologia contribui tanto na segurança alimentar como no entendimento de diversas patologias. O conhecimento dos agentes fúngicos presentes na pele de animais saudáveis e com patologias superficiais é importante para um controle eficaz dos riscos potenciais de infecção, principalmente quando associados a espécies confinadas, onde o desequilíbrio da microbiota ambiental pode ser decisivo nas interações entre o ambiente e os animais. Objetivou-se investigar a atividade antimicrobiana in vitro do óleo essencial de pétalas de Rosa alba L. sobre o crescimento microbiano de leveduras Candida albicans (ATCC 10231) e fungos filamentosos causadores de processos infecciosos oportunistas na pele de suínos, tais como Microsporum nanum (ATCC 11832), Trichophyton mentagrophytes (ATCC 9533), Trichophyton rubrum (ATCC 10218) e Trichophyton tonsurans (ATCC 10870). Os óleos foram extraídos pelo processo de hidrodestilação em aparelho de Clevenger, utilizando partes frescas das pétalas de rosa branca. Os testes microbiológicos foram realizados pelo método de difusão em ágar ( pour-plate ) com meio de cultura ágar Sabouraud-dextrose. Poços de 5 mm de diâmetro, produzidos no ágar, foram preenchidos com 15 μL do óleo essencial e, em seguida, as placas contendo os fungos filamentosos foram incubadas de forma invertida, a 28 ºC por 10 dias; e as placas contendo a levedura foram incubadas a 30 ºC por 48 horas. Passado este tempo, os halos de inibição tiveram seus diâmetros medidos. Os dados obtidos foram submetidos à análise de variância e as médias comparadas através do teste de Tukey ao nível de 5% de probabilidade. Verificou-se que a atividade do óleo essencial em estudo é significativa em relação ao crescimento das espécies microbianas testadas.Made available in DSpace on 2016-05-02T13:55:30Z (GMT). No. of bitstreams: 1 OswaldoHenriqueBarolliReis-Dissertacao.pdf: 707277 bytes, checksum: 3b71c133dbad0b57929d4b255619719c (MD5) Previous issue date: 2011-02-23application/pdfporUniversidade Jose do Rosario VellanoPrograma de Mestrado em Ciência AnimalUNIFENASBRZootecnia e Recursos Pesqueiroscandida albicansmicrosporum nanuntrichophyton sp.suínosdermatófitoscandida albicansmicrosporum nanumtrichophyton sp.pigsdermatophytesCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOSAção antifúngica do óleo essencial de Rosa alba L.Antifungal action of essential oil of Rosa alba L.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UNIFENASinstname:Universidade José do Rosário Vellano (UNIFENAS)instacron:UNIFENASORIGINALOswaldoHenriqueBarolliReis-Dissertacao.pdfapplication/pdf707277http://tede2.unifenas.br:8080/tede/bitstream/jspui/122/1/OswaldoHenriqueBarolliReis-Dissertacao.pdf3b71c133dbad0b57929d4b255619719cMD51jspui/1222016-05-02 10:55:30.573oai:tede2.unifenas.br:jspui/122Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.unifenas.br:8080/jspui/http://tede2.unifenas.br:8080/oai/requestbiblioteca@unifenas.br||biblioteca@unifenas.bropendoar:2016-05-02T13:55:30Biblioteca Digital de Teses e Dissertações da UNIFENAS - Universidade José do Rosário Vellano (UNIFENAS)false
dc.title.por.fl_str_mv Ação antifúngica do óleo essencial de Rosa alba L.
dc.title.alternative.eng.fl_str_mv Antifungal action of essential oil of Rosa alba L.
title Ação antifúngica do óleo essencial de Rosa alba L.
spellingShingle Ação antifúngica do óleo essencial de Rosa alba L.
Reis, Oswaldo Henrique Barolli
candida albicans
microsporum nanun
trichophyton sp.
suínos
dermatófitos
candida albicans
microsporum nanum
trichophyton sp.
pigs
dermatophytes
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS
title_short Ação antifúngica do óleo essencial de Rosa alba L.
title_full Ação antifúngica do óleo essencial de Rosa alba L.
title_fullStr Ação antifúngica do óleo essencial de Rosa alba L.
title_full_unstemmed Ação antifúngica do óleo essencial de Rosa alba L.
title_sort Ação antifúngica do óleo essencial de Rosa alba L.
author Reis, Oswaldo Henrique Barolli
author_facet Reis, Oswaldo Henrique Barolli
author_role author
dc.contributor.advisor1.fl_str_mv Miranda, Adélia Pereira
dc.contributor.advisor1ID.fl_str_mv CPF:03567810626
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9885391923578742
dc.contributor.referee1.fl_str_mv Fiorini, João Evangelista
dc.contributor.referee1ID.fl_str_mv CPF:05657512634
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/6320803631314170
dc.contributor.referee2.fl_str_mv Delu Filho, Nelson
dc.contributor.referee2ID.fl_str_mv CPF:06633253826
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/4783763747882936
dc.contributor.authorID.fl_str_mv CPF:85971642634
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3343193387948447
dc.contributor.author.fl_str_mv Reis, Oswaldo Henrique Barolli
contributor_str_mv Miranda, Adélia Pereira
Fiorini, João Evangelista
Delu Filho, Nelson
dc.subject.por.fl_str_mv candida albicans
microsporum nanun
trichophyton sp.
suínos
dermatófitos
topic candida albicans
microsporum nanun
trichophyton sp.
suínos
dermatófitos
candida albicans
microsporum nanum
trichophyton sp.
pigs
dermatophytes
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS
dc.subject.eng.fl_str_mv candida albicans
microsporum nanum
trichophyton sp.
pigs
dermatophytes
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS
description In swine culture, the study of mycology contributes to food security in the understanding of various diseases. A knowledge of fungal agents on the skin of healthy animals and surface pathologies is important for effective control of the potential risks of infection, especially when associated with confined species, where the imbalance in the microbiota of the environment can be crucial in the interactions between environment and animals. The objective was to investigate the in vitro antimicrobial activity of essential oil of Rosa alba L. petals on microbial growth of yeasts Candida albicans (ATCC 10231) and filamentous fungi that causes opportunistic infectious processes in the skin of pigs, such as Microsporum nanum (ATCC 11832), Trichophyton mentagrophytes (ATCC 9533), Trichophyton rubrum (ATCC 10218) and Trichophyton tonsurans (ATCC 10870). The oils were extracted by the process of hydrodistillation in Clevenger apparatus, using fresh white rose petals. Microbiological tests were performed by the agar diffusion method (pour-plate) with Sabouraud-dextrose. Wells 5 mm in diameter, produced in the agar, were filled with 15 μL of essential oil and the plates containing filamentous fungi were incubated in an inverted position at 28 °C for 10 days and the plates containing yeast were incubated at 30 °C for 48 hours. After this time, the inhibition circles were measured. The data were subjected to analysis of variance and means were compared by Tukey`s test at 5% probability. It was found that the activity of essential oil under study is significant in relation to the growth of microbial species tested.
publishDate 2011
dc.date.available.fl_str_mv 2011-11-16
dc.date.issued.fl_str_mv 2011-02-23
dc.date.accessioned.fl_str_mv 2016-05-02T13:55:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
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dc.identifier.citation.fl_str_mv REIS, Oswaldo Henrique Barolli. Antifungal action of essential oil of Rosa alba L.. 2011. 71 f. Dissertação (Mestrado em Zootecnia e Recursos Pesqueiros) - Universidade Jose do Rosario Vellano, Alfenas, 2011.
dc.identifier.uri.fl_str_mv http://tede2.unifenas.br:8080/jspui/handle/jspui/122
identifier_str_mv REIS, Oswaldo Henrique Barolli. Antifungal action of essential oil of Rosa alba L.. 2011. 71 f. Dissertação (Mestrado em Zootecnia e Recursos Pesqueiros) - Universidade Jose do Rosario Vellano, Alfenas, 2011.
url http://tede2.unifenas.br:8080/jspui/handle/jspui/122
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dc.publisher.none.fl_str_mv Universidade Jose do Rosario Vellano
dc.publisher.program.fl_str_mv Programa de Mestrado em Ciência Animal
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dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Zootecnia e Recursos Pesqueiros
publisher.none.fl_str_mv Universidade Jose do Rosario Vellano
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