Bioprospecção de extrato e substâncias isoladas de Acacia polyphylla e avaliação do potencial anti-inflamatório

• Ano de defesa: 2022
• Autor(a) principal: Silva, Daniele De Oliveira
• Orientador(a): Soares, Marisi Gomes
• Banca de defesa: Souza, Thiago Belarmino De, Ambrozin, Alessandra Regina Pepe
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Alfenas
• Programa de Pós-Graduação: Programa de Pós-Graduação em Química
• Departamento: Instituto de Química
• País: Brasil
• Palavras-chave em Português: Fabaceae; Inibição de PGE2; Ensaio ex vivo; Flavonoide; COX-2
• Área do conhecimento CNPq: QUIMICA::QUIMICA ORGANICA
• Link de acesso: https://repositorio.unifal-mg.edu.br/handle/123456789/2237

Plants are a great source of study for obtaining new substances with biological activities. In this context, due harmful adverse effects of current anti-inflammatory drugs, there is a need to identify new alternative substances. In recent studies by our research group, 47 different species of the Fabaceae family were evaluated for their anti-inflammatory potential, among which Acacia polyphylla showed significant results of anti-inflammatory activity in the ex vivo assay and also in the inhibition of edema of the ear in vivo. As a result, this work was intended to carry out a phytochemical study of the species A. polyphylla, contributing to the identification of new compounds responsible for the anti-inflammatory activity. For this, using traditional chromatographic techniques, several fractions of the A. polyphylla were obtained, which were evaluated in an ex vivo anti-inflammatory assay, using human blood. Among the sample tested, it was observed that the BH fraction had the best percentage of PGE2 inhibition (74.8%) when compared to the reference drugs dexamethasone and indomethacin (90.5 and 98.4%, respectively), demonstrating an significant potential for anti-inflammatory activity. The prostaglandin E2 (PGE2) is one of the main mediators of inflammation produced by the COX-1 and COX-2 enzyme pathways, which increases under inflammatory stimuli, which include infectious processes by bacteria, viruses, fungi and parasites, tissue necrosis, foreign bodies, reactions immunology, among others. Furthermore, part of the crude extract of A. polyphylla was analyzed by HPLC, allowing the isolation of two compounds, identified as astragalin and an unpublished compound. Another three compounds were isolated on the bench and confirmed by HPLC. In addition, the anti-inflammatory activity of the isolated compounds was investigated, where astragalin showed a significant PGE2 inhibition activity (48.3%). Through this study, it was possible to contribute to the phytochemical of A. polyphylla, identifying two compounds, including an unpublished compound and astragalin, reported for the first time in this specie, in addition to being able to confirming its anti- inflammatory potential through the ex vivo assay.