Avaliação dos efeitos da inibição da histona desacetilase 6 em linhagens celulares de melanoma

• Ano de defesa: 2022
• Autor(a) principal: Silva, João Marcos Oliveira Da
• Orientador(a): Gamero, Angel Mauricio Castro
• Banca de defesa: Junior, Robson José De Oliveira, Dias, Marcos Vinicios Salles
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Alfenas
• Programa de Pós-Graduação: Programa de Pós-Graduação em Biociências Aplicada à Saúde
• Departamento: Instituto de Ciências Biomédicas
• País: Brasil
• Palavras-chave em Português: HDAC6; WT-161; Melanoma; Câncer; Quimiorresistência
• Área do conhecimento CNPq: CIENCIAS DA SAUDE
• Link de acesso: https://repositorio.unifal-mg.edu.br/handle/123456789/2138

Malignant melanoma, among the types of skin cancer, has the highest number of deaths and a propensity for rapid progression. Several studies have shown that HDAC6 overexpression contributes to the development of melanoma and a worse prognosis for the patient. For this reason, this work aims to investigate the impact of HDAC6 inhibition in melanoma cell lines. We used three melanoma cell lines (CHL-1, WM1366 and SK-MEL-147) to evaluate the effects of HDAC6 inhibition with the WT-161 inhibitor, using cell viability assays, clonogenic capacity of the cells, apoptotic cell death, cell cycle and cell motility analyzes. As a response to WT-161 treatment, we found a decrease in proliferative and clonogenic capacity, a decrease in chemoresistance, an increase in the apoptotic population, a decrease in migratory/invasive capacity with an increase in adhesion capacity. We believe that by inhibiting HDAC6 we were able to elucidate part of a complex network that promotes and supports tumor progression.